DE69936201D1 - Enzymstabilisierung durch kationische, oberflächenaktive substanzen - Google Patents

Enzymstabilisierung durch kationische, oberflächenaktive substanzen

Info

Publication number
DE69936201D1
DE69936201D1 DE69936201T DE69936201T DE69936201D1 DE 69936201 D1 DE69936201 D1 DE 69936201D1 DE 69936201 T DE69936201 T DE 69936201T DE 69936201 T DE69936201 T DE 69936201T DE 69936201 D1 DE69936201 D1 DE 69936201D1
Authority
DE
Germany
Prior art keywords
active substances
surface active
cationic surface
enzyme stabilization
stabilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69936201T
Other languages
English (en)
Other versions
DE69936201T2 (de
Inventor
John W Shultz
Fen Huang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of DE69936201D1 publication Critical patent/DE69936201D1/de
Publication of DE69936201T2 publication Critical patent/DE69936201T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DE69936201T 1998-06-24 1999-06-23 Enzymstabilisierung durch kationische, oberflächenaktive substanzen Expired - Lifetime DE69936201T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US9053998P 1998-06-24 1998-06-24
US90539P 1998-06-24
US338174 1999-06-22
US09/338,174 US6242235B1 (en) 1998-06-24 1999-06-22 Polymerase stabilization by polyethoxylated amine surfactants
PCT/US1999/014217 WO1999067371A1 (en) 1998-06-24 1999-06-23 Enzyme stabilization by cationic surfactants

Publications (2)

Publication Number Publication Date
DE69936201D1 true DE69936201D1 (de) 2007-07-12
DE69936201T2 DE69936201T2 (de) 2008-02-07

Family

ID=26782391

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69936201T Expired - Lifetime DE69936201T2 (de) 1998-06-24 1999-06-23 Enzymstabilisierung durch kationische, oberflächenaktive substanzen

Country Status (12)

Country Link
US (1) US6242235B1 (de)
EP (2) EP1918368A3 (de)
JP (1) JP3673175B2 (de)
CN (1) CN1188518C (de)
AT (1) ATE363530T1 (de)
AU (1) AU761757B2 (de)
BR (1) BR9911396A (de)
CA (1) CA2335153C (de)
DE (1) DE69936201T2 (de)
ES (1) ES2288317T3 (de)
HK (1) HK1040262A1 (de)
WO (1) WO1999067371A1 (de)

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DE19743518A1 (de) 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US20030134292A1 (en) * 2001-10-30 2003-07-17 Farchaus Joseph W. Thermostable DNA polymerases and methods of making same
US6814845B2 (en) * 2001-11-21 2004-11-09 University Of Kansas Method for depositing an enzyme on an electrically conductive substrate
EP2345738A1 (de) * 2002-08-05 2011-07-20 Quanta Biosciences, Inc. Verbesserte Zusammensetzung zur In-vitro-Amplifikation von Nukleinsäuren
WO2004042003A2 (en) * 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit
ATE550441T1 (de) * 2005-09-06 2012-04-15 Gen Probe Inc Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
JP2009538146A (ja) * 2006-05-23 2009-11-05 モレキュラー ディテクション，インコーポレイテッド 室温で安定な分子診断用キット
JP5479895B2 (ja) * 2006-07-25 2014-04-23 アジレント・テクノロジーズ・インク Ｄｎａポリメラーゼの保存及び使用のための両性イオン洗剤
US7846703B2 (en) * 2006-10-02 2010-12-07 Takara Bio Inc. Method for enhancing polymerase activity
CA2713101C (en) * 2006-10-06 2016-09-13 Vandalia Research, Inc. Method for a continuous rapid thermal cycle system
US7972828B2 (en) * 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
EP1970440A1 (de) * 2007-03-06 2008-09-17 Qiagen GmbH Polymerasestabilisierung durch ionische Reinigungsmittel
EP2152864A1 (de) * 2007-06-13 2010-02-17 Amersham Biosciences Corp. Polymerasestabilisierung
US20110014676A1 (en) * 2007-06-29 2011-01-20 Battelle Memorial Institute Protein stabilization
EP2075331A1 (de) * 2007-12-28 2009-07-01 Qiagen GmbH Modifizierte Enzyme und deren Verwendung
US20090203022A1 (en) * 2008-02-07 2009-08-13 Arizona Board Of Regents For And On Behalf Of Arizona State University Analysis
US20120111727A1 (en) * 2009-01-26 2012-05-10 Vladislav Dolnik Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins
EP2393941A2 (de) * 2009-02-09 2011-12-14 Frederic Zenhausern Verbesserungen bei und in verbindung mit mikrofluidikvorrichtungen zur verarbeitung einer probe
GB0915796D0 (en) 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
CN102051352B (zh) * 2009-11-02 2012-07-04 刘萍 一种酶结合物稳定液
WO2012018639A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
EP2598660B1 (de) 2010-07-26 2017-03-15 Biomatrica, INC. Zusammensetzungen zur stabilisierung von dna, rna und proteinen in blut- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen
US8715987B2 (en) 2011-05-02 2014-05-06 New England Biolabs, Inc. Solubilized phospholipids for stabilizing nucleic acid polymerases
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
WO2012170908A1 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
US9464331B2 (en) * 2011-07-06 2016-10-11 Quest Diagnostics Investments Incorporated Direct amplification and detection of viral and bacterial pathogens
CN102559647A (zh) * 2011-12-31 2012-07-11 湖南利尔康生物有限公司 提高液体酶应用效果的方法
KR102078892B1 (ko) 2012-02-09 2020-02-19 라이프 테크놀로지스 코포레이션 접합된 중합체성 입자 및 그의 제조 방법
US8956816B2 (en) 2012-06-05 2015-02-17 Pacific Biosciences Of California, Inc. Methods and compositions for performing analytical operations
US9085761B1 (en) 2012-06-14 2015-07-21 Affymetrix, Inc. Methods and compositions for amplification of nucleic acids
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
WO2015061714A1 (en) 2013-10-25 2015-04-30 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
EP3154338B1 (de) 2014-06-10 2020-01-29 Biomatrica, INC. Stabilisierung von thrombozyten bei umgebungstemperatur
EP3172332B1 (de) * 2014-07-22 2020-10-14 Bio-rad Laboratories, Inc. Puffer zur verwendung mit polymerasen
WO2017004559A1 (en) 2015-07-02 2017-01-05 Life Technologies Corporation Conjugation of carboxyl functional hydrophilic beads
CN108350490B (zh) 2015-07-06 2022-06-21 生命技术公司 用于测序的底物和方法
JP6827048B2 (ja) 2015-12-08 2021-02-10 バイオマトリカ，インク． 赤血球沈降速度の低下
US10626383B2 (en) 2016-01-15 2020-04-21 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
US11268084B2 (en) 2017-08-08 2022-03-08 Thermo Fisher Scientific Baltics, UAB Glycerol-free formulations for reverse transcriptases
US11708598B2 (en) 2017-11-21 2023-07-25 Nanohelix Co., Ltd. Composition for polymerase reaction
JP2021504541A (ja) * 2017-11-29 2021-02-15 ビーエイエスエフ・ソシエタス・エウロパエアＢａｓｆ Ｓｅ 保存安定性酵素調製物、それらの製造およびそれらを使用する方法

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5352600A (en) 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
EP0295859B1 (de) * 1987-06-15 1994-11-17 Southern Cross Biotech Pty.Ltd. Herstellung von Proteinen in aktiver Form
USRE34157E (en) * 1988-06-23 1993-01-05 S. C. Johnson & Son, Inc. Hairspray with improved adhesion/removability upon washing
US5210036A (en) 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5356800A (en) * 1992-11-30 1994-10-18 Buckman Laboratories International, Inc. Stabilized liquid enzymatic compositions

Also Published As

Publication number Publication date
DE69936201T2 (de) 2008-02-07
EP1918368A2 (de) 2008-05-07
CA2335153A1 (en) 1999-12-29
JP3673175B2 (ja) 2005-07-20
CA2335153C (en) 2007-04-10
AU4829399A (en) 2000-01-10
JP2004500005A (ja) 2004-01-08
CN1188518C (zh) 2005-02-09
ATE363530T1 (de) 2007-06-15
EP1088060A1 (de) 2001-04-04
WO1999067371A1 (en) 1999-12-29
CN1315996A (zh) 2001-10-03
HK1040262A1 (en) 2002-05-31
US6242235B1 (en) 2001-06-05
EP1918368A3 (de) 2008-06-18
EP1088060B1 (de) 2007-05-30
EP1088060A4 (de) 2004-12-01
BR9911396A (pt) 2002-01-29
ES2288317T3 (es) 2008-01-01
AU761757B2 (en) 2003-06-12

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