ATE360096T1 - Reversibel modifizierte thermostabile enzyme für die in vitro-synthese und -amplifizierung von dna - Google Patents
Reversibel modifizierte thermostabile enzyme für die in vitro-synthese und -amplifizierung von dnaInfo
- Publication number
- ATE360096T1 ATE360096T1 AT02026712T AT02026712T ATE360096T1 AT E360096 T1 ATE360096 T1 AT E360096T1 AT 02026712 T AT02026712 T AT 02026712T AT 02026712 T AT02026712 T AT 02026712T AT E360096 T1 ATE360096 T1 AT E360096T1
- Authority
- AT
- Austria
- Prior art keywords
- modified thermostable
- activity
- enzyme
- thermostable enzyme
- amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01128725 | 2001-12-03 | ||
| US10/317,715 US7378262B2 (en) | 2001-12-03 | 2002-12-11 | Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360096T1 true ATE360096T1 (de) | 2007-05-15 |
Family
ID=32963733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02026712T ATE360096T1 (de) | 2001-12-03 | 2002-11-30 | Reversibel modifizierte thermostabile enzyme für die in vitro-synthese und -amplifizierung von dna |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7378262B2 (de) |
| EP (1) | EP1319719B8 (de) |
| JP (1) | JP3969581B2 (de) |
| AT (1) | ATE360096T1 (de) |
| CA (1) | CA2409775C (de) |
| DE (1) | DE60219579T2 (de) |
| ES (1) | ES2284769T3 (de) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| GB0502010D0 (en) * | 2005-02-01 | 2005-03-09 | Enigma Diagnostics Ltd | Biochemical reagents and their uses |
| US20070117114A1 (en) * | 2005-07-07 | 2007-05-24 | Quanta Biosciences, Inc. | Compositions and methods for increasing amplification efficiency |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| CN101050453B (zh) * | 2007-04-04 | 2010-06-30 | 无锡中德美联生物技术有限公司 | 一种热启动耐高温dna聚合酶及其制备方法 |
| EP2279263B1 (de) | 2008-04-30 | 2013-09-04 | Integrated Dna Technologies, Inc. | Tests auf rnase-h-basis mit modifizierten rna-monomeren |
| CA2802304C (en) | 2010-06-18 | 2016-01-12 | F.Hoffman-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
| CA2802239C (en) | 2010-06-18 | 2016-08-23 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
| JP5876479B2 (ja) | 2010-06-18 | 2016-03-02 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別を有するｄｎａポリメラーゼ |
| US8722378B2 (en) | 2010-06-18 | 2014-05-13 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| AU2011267421B2 (en) | 2010-06-18 | 2014-04-24 | F. Hoffmann-La Roche Ag | DNA polymerases with increased 3'-mismatch discrimination |
| EP2582808B1 (de) | 2010-06-18 | 2015-04-22 | Roche Diagniostics GmbH | Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen |
| EP2582806B1 (de) | 2010-06-18 | 2015-12-16 | Roche Diagnostics GmbH | Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz |
| ES2536252T3 (es) | 2010-06-18 | 2015-05-21 | F. Hoffmann-La Roche Ag | ADN polimerasas con diferenciación de desapareamientos 3' aumentada |
| EP3578205A1 (de) | 2010-08-06 | 2019-12-11 | ModernaTX, Inc. | Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung |
| CA3162352A1 (en) | 2010-10-01 | 2012-04-05 | Modernatx, Inc. | Modified nucleosides, nucleotides, and nucleic acids, and uses thereof |
| US8765435B2 (en) | 2011-02-15 | 2014-07-01 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| DE12722942T1 (de) | 2011-03-31 | 2021-09-30 | Modernatx, Inc. | Freisetzung und formulierung von manipulierten nukleinsäuren |
| WO2012139748A1 (en) | 2011-04-11 | 2012-10-18 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| WO2013013822A1 (en) | 2011-07-28 | 2013-01-31 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| PT3682905T (pt) | 2011-10-03 | 2022-04-07 | Modernatx Inc | Nucleósidos, nucleótidos e ácidos nucleicos modificados e respetivas utilizações |
| EP2788481B1 (de) | 2011-12-08 | 2018-03-07 | Roche Diagnostics GmbH | Dna-polymerasen mit verbesserter aktivität |
| ES2716277T3 (es) | 2011-12-08 | 2019-06-11 | Hoffmann La Roche | ADN polimerasas con actividad mejorada |
| ES2569723T3 (es) | 2011-12-08 | 2016-05-12 | F. Hoffmann-La Roche Ag | Polimerasas de DNA con actividad mejorada |
| LT2791160T (lt) | 2011-12-16 | 2022-06-10 | Modernatx, Inc. | Modifikuotos mrnr sudėtys |
| US9303079B2 (en) | 2012-04-02 | 2016-04-05 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| CA2868996A1 (en) | 2012-04-02 | 2013-10-10 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| PL2922554T3 (pl) | 2012-11-26 | 2022-06-20 | Modernatx, Inc. | Na zmodyfikowany na końcach |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| CA2923029A1 (en) | 2013-09-03 | 2015-03-12 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
| JP2016538829A (ja) | 2013-10-03 | 2016-12-15 | モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． | 低密度リポタンパク質受容体をコードするポリヌクレオチド |
| AU2015289583A1 (en) | 2014-07-16 | 2017-02-02 | Modernatx, Inc. | Chimeric polynucleotides |
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| LT3394093T (lt) | 2015-12-23 | 2022-04-25 | Modernatx, Inc. | Ox40 ligandus koduojančių polinukleotidų naudojimo būdai |
| WO2017120612A1 (en) | 2016-01-10 | 2017-07-13 | Modernatx, Inc. | Therapeutic mrnas encoding anti ctla-4 antibodies |
| JP7246930B2 (ja) | 2016-05-18 | 2023-03-28 | モデルナティエックス インコーポレイテッド | インターロイキン－１２（ｉｌ１２）をコードするポリヌクレオチドおよびその使用 |
| CN109476718B (zh) | 2016-05-18 | 2023-07-04 | 莫得纳特斯公司 | 编码免疫调节多肽的mrna的组合及其用途 |
| WO2018231990A2 (en) | 2017-06-14 | 2018-12-20 | Modernatx, Inc. | Polynucleotides encoding methylmalonyl-coa mutase |
| CN107312762B (zh) * | 2017-06-23 | 2020-08-11 | 苏州点晶生物科技有限公司 | 一种化学修饰ung酶及其修饰方法和应用 |
| JP2022500046A (ja) | 2018-09-13 | 2022-01-04 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ |
| US20220056498A1 (en) | 2019-01-25 | 2022-02-24 | President And Fellows Of Harvard College | Compositions and method for synthesizing nucleic acids |
| CA3161183A1 (en) | 2019-12-12 | 2021-06-17 | President And Fellows Of Harvard College | Compositions and methods for light-directed biomolecular barcoding |
| EP4139456A1 (de) | 2020-04-22 | 2023-03-01 | President And Fellows Of Harvard College | Isothermische verfahren, zusammensetzungen, kits und systeme zum nachweis von nukleinsäuren |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| US5512462A (en) * | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| AU5305296A (en) * | 1995-03-07 | 1996-09-23 | Biomolecular Assays, Inc. | Pressure cycling reactor |
| CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| GB9920194D0 (en) * | 1999-08-27 | 1999-10-27 | Advanced Biotech Ltd | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
| ATE286981T1 (de) * | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| US20030049614A1 (en) * | 1999-10-06 | 2003-03-13 | Holly Hurlbut Hogrefe | Compositions for dna amplification, synthesis, and mutagenesis |
-
2002
- 2002-11-28 CA CA2409775A patent/CA2409775C/en not_active Expired - Lifetime
- 2002-11-30 ES ES02026712T patent/ES2284769T3/es not_active Expired - Lifetime
- 2002-11-30 AT AT02026712T patent/ATE360096T1/de active
- 2002-11-30 EP EP02026712A patent/EP1319719B8/de not_active Expired - Lifetime
- 2002-11-30 DE DE60219579T patent/DE60219579T2/de not_active Expired - Lifetime
- 2002-12-03 JP JP2002351736A patent/JP3969581B2/ja not_active Expired - Lifetime
- 2002-12-11 US US10/317,715 patent/US7378262B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP3969581B2 (ja) | 2007-09-05 |
| CA2409775A1 (en) | 2003-06-03 |
| US20040115639A1 (en) | 2004-06-17 |
| US7378262B2 (en) | 2008-05-27 |
| ES2284769T3 (es) | 2007-11-16 |
| EP1319719A1 (de) | 2003-06-18 |
| DE60219579T2 (de) | 2008-01-03 |
| EP1319719B8 (de) | 2008-08-27 |
| EP1319719B1 (de) | 2007-04-18 |
| JP2003199592A (ja) | 2003-07-15 |
| DE60219579D1 (de) | 2007-05-31 |
| CA2409775C (en) | 2010-07-13 |
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| UEP | Publication of translation of european patent specification |
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