DE60219579D1 - Reversibel modifizierte thermostabile Enzyme für die in vitro-Synthese und -Amplifizierung von DNA - Google Patents
Reversibel modifizierte thermostabile Enzyme für die in vitro-Synthese und -Amplifizierung von DNAInfo
- Publication number
- DE60219579D1 DE60219579D1 DE60219579T DE60219579T DE60219579D1 DE 60219579 D1 DE60219579 D1 DE 60219579D1 DE 60219579 T DE60219579 T DE 60219579T DE 60219579 T DE60219579 T DE 60219579T DE 60219579 D1 DE60219579 D1 DE 60219579D1
- Authority
- DE
- Germany
- Prior art keywords
- modified thermostable
- activity
- enzyme
- thermostable enzyme
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01128725 | 2001-12-03 | ||
EP01128725 | 2001-12-03 | ||
US10/317,715 US7378262B2 (en) | 2001-12-03 | 2002-12-11 | Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60219579D1 true DE60219579D1 (de) | 2007-05-31 |
DE60219579T2 DE60219579T2 (de) | 2008-01-03 |
Family
ID=32963733
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60219579T Expired - Lifetime DE60219579T2 (de) | 2001-12-03 | 2002-11-30 | Reversibel modifizierte thermostabile Enzyme für die in vitro-Synthese und -Amplifizierung von DNA |
Country Status (7)
Country | Link |
---|---|
US (1) | US7378262B2 (de) |
EP (1) | EP1319719B8 (de) |
JP (1) | JP3969581B2 (de) |
AT (1) | ATE360096T1 (de) |
CA (1) | CA2409775C (de) |
DE (1) | DE60219579T2 (de) |
ES (1) | ES2284769T3 (de) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
GB0502010D0 (en) * | 2005-02-01 | 2005-03-09 | Enigma Diagnostics Ltd | Biochemical reagents and their uses |
EP1920064B1 (de) * | 2005-07-07 | 2014-12-24 | Quanta Biosciences, Inc. | Zusammensetzungen und verfahren zur erhöhung der amplifikationseffizienz |
US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
CN101050453B (zh) * | 2007-04-04 | 2010-06-30 | 无锡中德美联生物技术有限公司 | 一种热启动耐高温dna聚合酶及其制备方法 |
JP5539325B2 (ja) | 2008-04-30 | 2014-07-02 | インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド | 修飾されたrna単量体を用いるrnアーゼhを基礎とするアッセイ |
WO2011157435A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
CN103025868B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
ES2536978T3 (es) | 2010-06-18 | 2015-06-01 | F. Hoffmann-La Roche Ag | ADN polimerasas con discriminación incrementada de no correspondencias 3' |
CA2801997C (en) | 2010-06-18 | 2016-05-31 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
WO2011157430A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
US8735121B2 (en) | 2010-06-18 | 2014-05-27 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-match discrimination |
US8722380B2 (en) | 2010-06-18 | 2014-05-13 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
US8759062B2 (en) | 2010-06-18 | 2014-06-24 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′- mismatch discrimination |
US8822663B2 (en) | 2010-08-06 | 2014-09-02 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
HUE058896T2 (hu) | 2010-10-01 | 2022-09-28 | Modernatx Inc | N1-metil-pszeudo-uracilt tartalmazó ribonukleinsavak és azok felhasználásai |
EP2675897B1 (de) | 2011-02-15 | 2016-05-11 | Roche Diagnostics GmbH | Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz |
US8710200B2 (en) | 2011-03-31 | 2014-04-29 | Moderna Therapeutics, Inc. | Engineered nucleic acids encoding a modified erythropoietin and their expression |
ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
ES2553400T3 (es) | 2011-07-28 | 2015-12-09 | F. Hoffmann-La Roche Ag | ADN polimerasas con actividad mejorada |
US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
RS62993B1 (sr) | 2011-10-03 | 2022-03-31 | Modernatx Inc | Modifikovani nukleozidi, nukleotidi, i nukleinske kiseline, i njihove upotrebe |
JP6140182B2 (ja) | 2011-12-08 | 2017-05-31 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 改善された活性を有するdnaポリメラーゼ |
WO2013083262A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
CN104114572A (zh) | 2011-12-16 | 2014-10-22 | 现代治疗公司 | 经修饰的核苷、核苷酸和核酸组合物 |
US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
US9303079B2 (en) | 2012-04-02 | 2016-04-05 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
DE18200782T1 (de) | 2012-04-02 | 2021-10-21 | Modernatx, Inc. | Modifizierte polynukleotide zur herstellung von proteinen im zusammenhang mit erkrankungen beim menschen |
US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
EP4074834A1 (de) | 2012-11-26 | 2022-10-19 | ModernaTX, Inc. | Am kettenende modifizierte rna |
US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
AU2014315287A1 (en) | 2013-09-03 | 2015-03-12 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
MX2016004249A (es) | 2013-10-03 | 2016-11-08 | Moderna Therapeutics Inc | Polinulcleotidos que codifican el receptor de lipoproteina de baja densidad. |
WO2016011226A1 (en) | 2014-07-16 | 2016-01-21 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
PT3394093T (pt) | 2015-12-23 | 2022-05-30 | Modernatx Inc | Métodos de utilização de polinucleotídeos que codificam ligandos ox40 |
EP3400023A1 (de) | 2016-01-10 | 2018-11-14 | ModernaTX, Inc. | Therapeutische mrnas zur codierung von anti-ctla-4-antikörpern |
MX2018013509A (es) | 2016-05-18 | 2019-03-28 | Modernatx Inc | Polinucleotidos que codifican interleucina-12 (il12) y sus usos de los mismos. |
KR102482867B1 (ko) | 2016-05-18 | 2023-01-02 | 모더나티엑스, 인크. | 면역 조정 폴리펩타이드를 암호화하는 mRNA의 조합물 및 이의 용도 |
US20200131498A1 (en) | 2017-06-14 | 2020-04-30 | Modernatx, Inc. | Polynucleotides encoding methylmalonyl-coa mutase |
CN107312762B (zh) * | 2017-06-23 | 2020-08-11 | 苏州点晶生物科技有限公司 | 一种化学修饰ung酶及其修饰方法和应用 |
US11739306B2 (en) | 2018-09-13 | 2023-08-29 | Roche Molecular Systems, Inc. | Mutant DNA polymerase(s) with improved strand displacement ability |
EP3914734B1 (de) | 2019-01-25 | 2024-03-13 | President and Fellows of Harvard College | Zusammensetzungen und verfahren zur sequenzierung von nukleinsäuren |
AU2020400056A1 (en) | 2019-12-12 | 2022-07-07 | President And Fellows Of Harvard College | Compositions and methods for light-directed biomolecular barcoding |
CA3176545A1 (en) | 2020-04-22 | 2021-10-28 | President And Fellows Of Harvard College | Isothermal methods, compositions, kits, and systems for detecting nucleic acids |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
US5512462A (en) * | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
DE69615165T2 (de) * | 1995-03-07 | 2002-05-16 | B B I Bioseq Inc | Verfahren zur regelung von enzymatischen reaktionen |
CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
GB9920194D0 (en) * | 1999-08-27 | 1999-10-27 | Advanced Biotech Ltd | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
ATE286981T1 (de) * | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
US20030049614A1 (en) * | 1999-10-06 | 2003-03-13 | Holly Hurlbut Hogrefe | Compositions for dna amplification, synthesis, and mutagenesis |
-
2002
- 2002-11-28 CA CA2409775A patent/CA2409775C/en not_active Expired - Lifetime
- 2002-11-30 AT AT02026712T patent/ATE360096T1/de active
- 2002-11-30 DE DE60219579T patent/DE60219579T2/de not_active Expired - Lifetime
- 2002-11-30 ES ES02026712T patent/ES2284769T3/es not_active Expired - Lifetime
- 2002-11-30 EP EP02026712A patent/EP1319719B8/de not_active Expired - Lifetime
- 2002-12-03 JP JP2002351736A patent/JP3969581B2/ja not_active Expired - Lifetime
- 2002-12-11 US US10/317,715 patent/US7378262B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP1319719B8 (de) | 2008-08-27 |
ES2284769T3 (es) | 2007-11-16 |
CA2409775C (en) | 2010-07-13 |
JP2003199592A (ja) | 2003-07-15 |
JP3969581B2 (ja) | 2007-09-05 |
EP1319719B1 (de) | 2007-04-18 |
EP1319719A1 (de) | 2003-06-18 |
DE60219579T2 (de) | 2008-01-03 |
CA2409775A1 (en) | 2003-06-03 |
US7378262B2 (en) | 2008-05-27 |
US20040115639A1 (en) | 2004-06-17 |
ATE360096T1 (de) | 2007-05-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |