ATE286981T1 - Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation - Google Patents
Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikationInfo
- Publication number
- ATE286981T1 ATE286981T1 AT99119268T AT99119268T ATE286981T1 AT E286981 T1 ATE286981 T1 AT E286981T1 AT 99119268 T AT99119268 T AT 99119268T AT 99119268 T AT99119268 T AT 99119268T AT E286981 T1 ATE286981 T1 AT E286981T1
- Authority
- AT
- Austria
- Prior art keywords
- thermostable
- nucleic acid
- enzyme
- accuracy
- increases
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 title abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title 1
- 238000001668 nucleic acid synthesis Methods 0.000 title 1
- 108060002716 Exonuclease Proteins 0.000 abstract 3
- 102000013165 exonuclease Human genes 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 3
- 241000205042 Archaeoglobus fulgidus Species 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP99119268A EP1088891B1 (de) | 1999-09-28 | 1999-09-28 | Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE286981T1 true ATE286981T1 (de) | 2005-01-15 |
Family
ID=8239082
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99119268T ATE286981T1 (de) | 1999-09-28 | 1999-09-28 | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| AT00969312T ATE335825T1 (de) | 1999-09-28 | 2000-09-27 | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00969312T ATE335825T1 (de) | 1999-09-28 | 2000-09-27 | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der in vitro nucleinsäuresynthese und amplifikation |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7030220B1 (de) |
| EP (2) | EP1088891B1 (de) |
| JP (1) | JP3655240B2 (de) |
| CN (1) | CN1185347C (de) |
| AT (2) | ATE286981T1 (de) |
| AU (1) | AU777229B2 (de) |
| CA (1) | CA2351634C (de) |
| DE (2) | DE69923180T2 (de) |
| ES (1) | ES2269189T3 (de) |
| IL (1) | IL143141A0 (de) |
| NO (1) | NO20012561L (de) |
| NZ (1) | NZ511759A (de) |
| RU (1) | RU2260055C2 (de) |
| WO (1) | WO2001023583A2 (de) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE286981T1 (de) | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| EP1277841B1 (de) * | 2001-07-11 | 2009-08-26 | Roche Diagnostics GmbH | Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung |
| EP1275735A1 (de) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
| CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| US20030180741A1 (en) * | 2001-12-21 | 2003-09-25 | Holly Hogrefe | High fidelity DNA polymerase compositions and uses therefor |
| US9181534B1 (en) | 2001-12-21 | 2015-11-10 | Agilent Technologies Inc. | High fidelity DNA polymerase compositions and uses thereof |
| US7932070B2 (en) | 2001-12-21 | 2011-04-26 | Agilent Technologies, Inc. | High fidelity DNA polymerase compositions and uses therefor |
| GB0208768D0 (en) | 2002-04-17 | 2002-05-29 | Univ Newcastle | DNA polymerases |
| US8283148B2 (en) | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
| EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
| US20080213762A1 (en) * | 2004-12-08 | 2008-09-04 | Takeshi Yamamoto | Method of Gene Sequence Examination |
| US7922177B2 (en) * | 2005-05-18 | 2011-04-12 | Diamond Game Enterprises, Inc. | Ticket strips that encourage multiple ticket purchasing |
| WO2007057167A2 (en) * | 2005-11-18 | 2007-05-24 | Bioline Limited | A method for enhancing enzymatic dna polymerase reactions |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8409805B2 (en) | 2009-02-13 | 2013-04-02 | Asuragen, Inc. | Method of amplification of GC-rich DNA templates |
| KR20110138387A (ko) | 2009-03-24 | 2011-12-27 | 아수라겐, 인크. | Ｆｍｒ１ 및 ｆｍｒ２ 유전자의 ５´ 비번역 영역을 규명하기 위한 ｐｃｒ 방법 |
| WO2010119407A1 (en) * | 2009-04-16 | 2010-10-21 | Koninklijke Philips Electronics N.V. | Methods for nucleic acid quantification |
| GB0915796D0 (en) * | 2009-09-09 | 2009-10-07 | Fermentas Uab | Polymerase compositions and uses |
| US9238832B2 (en) * | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
| WO2011157436A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| CA2802239C (en) | 2010-06-18 | 2016-08-23 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
| EP2582807B1 (de) | 2010-06-18 | 2015-03-18 | Roche Diagnostics GmbH | Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen |
| EP2582808B1 (de) | 2010-06-18 | 2015-04-22 | Roche Diagniostics GmbH | Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen |
| KR101622442B1 (ko) | 2010-06-18 | 2016-05-19 | 에프. 호프만-라 로슈 아게 | ３’―미스매치 구별이 증가된 ｄｎａ 중합효소 |
| WO2011157438A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increasesed 3'-mismatch discrimination |
| CN103025870B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| CN103003418B (zh) | 2010-06-18 | 2015-08-26 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| US8765435B2 (en) | 2011-02-15 | 2014-07-01 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| CA2831180C (en) | 2011-04-11 | 2017-02-14 | Keith Bauer | Dna polymerases with improved activity |
| CA2839964C (en) | 2011-07-28 | 2019-03-12 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| EP2559774A1 (de) * | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes |
| CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| EP2788479B1 (de) | 2011-12-08 | 2016-02-10 | Roche Diagnostics GmbH | Dna-polymerasen mit verbesserter aktivität |
| JP6224613B2 (ja) | 2011-12-08 | 2017-11-01 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| EP2888688B1 (de) | 2012-07-20 | 2019-09-04 | Asuragen, INC. | Umfassende fmr1-genotypisierung |
| GB201611469D0 (en) * | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| WO2020053327A1 (en) | 2018-09-13 | 2020-03-19 | F. Hoffmann-La Roche Ag | Mutant dna polymerase(s) with improved strand displacement ability |
| WO2022071888A1 (en) * | 2020-10-02 | 2022-04-07 | National University Of Singapore | A dna assembly mix and method of uses thereof |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5358990A (en) * | 1989-03-21 | 1990-10-22 | Collaborative Research Inc. | Multiplex dna diagnostic test |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
| AU6418294A (en) * | 1993-03-30 | 1994-10-24 | United States Biochemical Corporation | Use of exonuclease in dna sequencing |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| AU717156B2 (en) * | 1995-12-27 | 2000-03-16 | Takara Bio Inc. | Novel DNA polymerase |
| WO1998045452A2 (en) * | 1997-04-08 | 1998-10-15 | The Rockfeller University | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
| US6238905B1 (en) * | 1997-09-12 | 2001-05-29 | University Technology Corporation | Thermophilic polymerase III holoenzyme |
| DE19810879A1 (de) * | 1998-03-13 | 1999-09-16 | Roche Diagnostics Gmbh | Polymerasenchimären |
| DE19813317A1 (de) * | 1998-03-26 | 1999-09-30 | Roche Diagnostics Gmbh | Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR |
| JP2002543795A (ja) | 1999-05-12 | 2002-12-24 | インビトロゲン・コーポレーション | 核酸合成の感度および特異性の増大のための組成物および方法 |
| ATE286981T1 (de) | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| EP1275735A1 (de) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
-
1999
- 1999-09-28 AT AT99119268T patent/ATE286981T1/de not_active IP Right Cessation
- 1999-09-28 DE DE69923180T patent/DE69923180T2/de not_active Expired - Fee Related
- 1999-09-28 EP EP99119268A patent/EP1088891B1/de not_active Expired - Lifetime
-
2000
- 2000-09-27 CN CNB008030588A patent/CN1185347C/zh not_active Expired - Lifetime
- 2000-09-27 EP EP00969312A patent/EP1144653B1/de not_active Expired - Lifetime
- 2000-09-27 CA CA002351634A patent/CA2351634C/en not_active Expired - Lifetime
- 2000-09-27 IL IL14314100A patent/IL143141A0/xx unknown
- 2000-09-27 NZ NZ511759A patent/NZ511759A/en not_active IP Right Cessation
- 2000-09-27 US US09/856,850 patent/US7030220B1/en not_active Expired - Lifetime
- 2000-09-27 AT AT00969312T patent/ATE335825T1/de not_active IP Right Cessation
- 2000-09-27 DE DE60029927T patent/DE60029927T2/de not_active Expired - Lifetime
- 2000-09-27 AU AU79077/00A patent/AU777229B2/en not_active Ceased
- 2000-09-27 WO PCT/EP2000/009423 patent/WO2001023583A2/en not_active Ceased
- 2000-09-27 RU RU2001117496/13A patent/RU2260055C2/ru not_active IP Right Cessation
- 2000-09-27 ES ES00969312T patent/ES2269189T3/es not_active Expired - Lifetime
- 2000-09-27 JP JP2001526965A patent/JP3655240B2/ja not_active Expired - Lifetime
-
2001
- 2001-05-25 NO NO20012561A patent/NO20012561L/no not_active Application Discontinuation
-
2005
- 2005-09-29 US US11/241,116 patent/US7410782B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP3655240B2 (ja) | 2005-06-02 |
| DE60029927D1 (de) | 2006-09-21 |
| CN1185347C (zh) | 2005-01-19 |
| US7410782B2 (en) | 2008-08-12 |
| JP2003510084A (ja) | 2003-03-18 |
| NZ511759A (en) | 2003-12-19 |
| DE69923180T2 (de) | 2006-01-12 |
| EP1144653B1 (de) | 2006-08-09 |
| WO2001023583A2 (en) | 2001-04-05 |
| WO2001023583A3 (en) | 2001-10-25 |
| AU777229B2 (en) | 2004-10-07 |
| NO20012561L (no) | 2001-07-18 |
| ATE335825T1 (de) | 2006-09-15 |
| CA2351634C (en) | 2007-02-20 |
| ES2269189T3 (es) | 2007-04-01 |
| RU2260055C2 (ru) | 2005-09-10 |
| AU7907700A (en) | 2001-04-30 |
| US20060078928A1 (en) | 2006-04-13 |
| DE69923180D1 (de) | 2005-02-17 |
| EP1088891B1 (de) | 2005-01-12 |
| IL143141A0 (en) | 2002-04-21 |
| EP1088891A1 (de) | 2001-04-04 |
| EP1144653A2 (de) | 2001-10-17 |
| US7030220B1 (en) | 2006-04-18 |
| CA2351634A1 (en) | 2001-04-05 |
| CN1343254A (zh) | 2002-04-03 |
| NO20012561D0 (no) | 2001-05-25 |
| DE60029927T2 (de) | 2007-03-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |