NO20012561D0 - Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro - Google Patents

Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro

Info

Publication number
NO20012561D0
NO20012561D0 NO20012561A NO20012561A NO20012561D0 NO 20012561 D0 NO20012561 D0 NO 20012561D0 NO 20012561 A NO20012561 A NO 20012561A NO 20012561 A NO20012561 A NO 20012561A NO 20012561 D0 NO20012561 D0 NO 20012561D0
Authority
NO
Norway
Prior art keywords
thermostable
nucleic acid
amplification
fidelity
vitro
Prior art date
Application number
NO20012561A
Other languages
English (en)
Other versions
NO20012561L (no
Inventor
Waltraud Ankenbauer
Frank Laue
Harald Sobek
Michael Greif
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of NO20012561D0 publication Critical patent/NO20012561D0/no
Publication of NO20012561L publication Critical patent/NO20012561L/no

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
NO20012561A 1999-09-28 2001-05-25 Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro NO20012561L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99119268A EP1088891B1 (en) 1999-09-28 1999-09-28 Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro
PCT/EP2000/009423 WO2001023583A2 (en) 1999-09-28 2000-09-27 Thermostable enzyme promoting the fidelity of thermostable dna polymerases- for improvement of nucleic acid synthesis and amplification in vitro

Publications (2)

Publication Number Publication Date
NO20012561D0 true NO20012561D0 (no) 2001-05-25
NO20012561L NO20012561L (no) 2001-07-18

Family

ID=8239082

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20012561A NO20012561L (no) 1999-09-28 2001-05-25 Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro

Country Status (14)

Country Link
US (2) US7030220B1 (no)
EP (2) EP1088891B1 (no)
JP (1) JP3655240B2 (no)
CN (1) CN1185347C (no)
AT (2) ATE286981T1 (no)
AU (1) AU777229B2 (no)
CA (1) CA2351634C (no)
DE (2) DE69923180T2 (no)
ES (1) ES2269189T3 (no)
IL (1) IL143141A0 (no)
NO (1) NO20012561L (no)
NZ (1) NZ511759A (no)
RU (1) RU2260055C2 (no)
WO (1) WO2001023583A2 (no)

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EP1088891B1 (en) * 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro
EP1277841B1 (en) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH New composition and method for hot start nucleic acid amplification
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
US7932070B2 (en) 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
US20030180741A1 (en) * 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
US9181534B1 (en) 2001-12-21 2015-11-10 Agilent Technologies Inc. High fidelity DNA polymerase compositions and uses thereof
GB0208768D0 (en) 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
US8283148B2 (en) 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
EP1502958A1 (en) * 2003-08-01 2005-02-02 Roche Diagnostics GmbH New detection format for hot start real time polymerase chain reaction
US20080213762A1 (en) * 2004-12-08 2008-09-04 Takeshi Yamamoto Method of Gene Sequence Examination
US7922177B2 (en) * 2005-05-18 2011-04-12 Diamond Game Enterprises, Inc. Ticket strips that encourage multiple ticket purchasing
AU2006314757A1 (en) * 2005-11-18 2007-05-24 Bioline Limited A method for enhancing enzymatic DNA polymerase reactions
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8409805B2 (en) * 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
EP2411532B1 (en) * 2009-03-24 2017-04-05 Asuragen, Inc. Pcr methods for characterizing the 5' untranslated region of the fmr1 and fmr2 genes
CN102395977B (zh) * 2009-04-16 2014-10-22 皇家飞利浦电子股份有限公司 核酸定量的方法
GB0915796D0 (en) 2009-09-09 2009-10-07 Fermentas Uab Polymerase compositions and uses
US9238832B2 (en) * 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8722379B2 (en) 2010-06-18 2014-05-13 Roche Medical Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
KR101622442B1 (ko) 2010-06-18 2016-05-19 에프. 호프만-라 로슈 아게 ３’―미스매치 구별이 증가된 ｄｎａ 중합효소
CA2802302C (en) 2010-06-18 2016-04-26 F. Hoffman-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CA2802000C (en) 2010-06-18 2016-08-16 F. Hoffmann-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
JP5876477B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
US8722378B2 (en) 2010-06-18 2014-05-13 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
JP5847201B2 (ja) 2011-02-15 2016-01-20 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
CN103492559B (zh) 2011-04-11 2016-07-06 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
EP2559774A1 (en) * 2011-08-17 2013-02-20 Roche Diagniostics GmbH Improved method for amplification of target nucleic acids using a multi-primer approach
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
CN103987844B (zh) 2011-12-08 2016-01-20 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
JP6224613B2 (ja) 2011-12-08 2017-11-01 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
EP2888688B1 (en) 2012-07-20 2019-09-04 Asuragen, INC. Comprehensive fmr1 genotyping
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CN112703248A (zh) 2018-09-13 2021-04-23 豪夫迈·罗氏有限公司 具有改良链置换能力的突变型dna聚合酶
CN116391042A (zh) * 2020-10-02 2023-07-04 新加坡国立大学 Dna组装混合物和其使用方法

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AU5640090A (en) * 1989-03-21 1990-11-05 Collaborative Research Inc. A dna diagnostic test using an exonuclease activity
US6410277B1 (en) * 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
WO1994023066A1 (en) 1993-03-30 1994-10-13 United States Biochemical Corporation Use of exonuclease in dna sequencing
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
EP0870832A4 (en) * 1995-12-27 2001-08-22 Takara Shuzo Co NEW DNA POLYMERASE
AU7683998A (en) * 1997-04-08 1998-10-30 Rockfeller University, The Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US6238905B1 (en) * 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
DE19813317A1 (de) * 1998-03-26 1999-09-30 Roche Diagnostics Gmbh Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR
JP2002543795A (ja) 1999-05-12 2002-12-24 インビトロゲン・コーポレーション 核酸合成の感度および特異性の増大のための組成物および方法
EP1088891B1 (en) 1999-09-28 2005-01-12 Roche Diagnostics GmbH Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification

Also Published As

Publication number Publication date
DE69923180T2 (de) 2006-01-12
NZ511759A (en) 2003-12-19
NO20012561L (no) 2001-07-18
WO2001023583A3 (en) 2001-10-25
CN1343254A (zh) 2002-04-03
CA2351634C (en) 2007-02-20
IL143141A0 (en) 2002-04-21
RU2260055C2 (ru) 2005-09-10
JP2003510084A (ja) 2003-03-18
US20060078928A1 (en) 2006-04-13
EP1144653B1 (en) 2006-08-09
ATE335825T1 (de) 2006-09-15
CA2351634A1 (en) 2001-04-05
ES2269189T3 (es) 2007-04-01
DE60029927D1 (de) 2006-09-21
CN1185347C (zh) 2005-01-19
US7030220B1 (en) 2006-04-18
WO2001023583A2 (en) 2001-04-05
US7410782B2 (en) 2008-08-12
DE60029927T2 (de) 2007-03-08
EP1144653A2 (en) 2001-10-17
DE69923180D1 (de) 2005-02-17
JP3655240B2 (ja) 2005-06-02
EP1088891A1 (en) 2001-04-04
ATE286981T1 (de) 2005-01-15
EP1088891B1 (en) 2005-01-12
AU7907700A (en) 2001-04-30
AU777229B2 (en) 2004-10-07

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