WO2004022574A3 - Verbesserte verfahren zur synthese von nukleinsäuren - Google Patents

Verbesserte verfahren zur synthese von nukleinsäuren Download PDF

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Publication number
WO2004022574A3
WO2004022574A3 PCT/EP2003/009756 EP0309756W WO2004022574A3 WO 2004022574 A3 WO2004022574 A3 WO 2004022574A3 EP 0309756 W EP0309756 W EP 0309756W WO 2004022574 A3 WO2004022574 A3 WO 2004022574A3
Authority
WO
WIPO (PCT)
Prior art keywords
synthesis
nucleic acids
improved methods
polymerase
nucleic acid
Prior art date
Application number
PCT/EP2003/009756
Other languages
English (en)
French (fr)
Other versions
WO2004022574A2 (de
Inventor
Guido Krupp
Original Assignee
Artus Ges Fuer Molekularbiolog
Guido Krupp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Artus Ges Fuer Molekularbiolog, Guido Krupp filed Critical Artus Ges Fuer Molekularbiolog
Priority to US10/526,487 priority Critical patent/US20060172299A1/en
Priority to EP03750483A priority patent/EP1537236A2/de
Priority to AU2003270143A priority patent/AU2003270143A1/en
Publication of WO2004022574A2 publication Critical patent/WO2004022574A2/de
Publication of WO2004022574A3 publication Critical patent/WO2004022574A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

Die Erfindung betrifft ein Verfahren zur Synthese von Nukleinsäuren, bei dem man eine Polymerase, eine Nukleinsäure, die als Matrize für die Polymerase dienen kann, NTPs und Mn2+ unter Bedingungen inkubiert, welche die Synthese eines Nukleinsäurestranges ermöglichen, welches dadurch gekennzeichnet ist, dass die Bedingungen ein Molverhältnis von Mn2+/NTP von nicht mehr als 0,7 umfassen.
PCT/EP2003/009756 2002-09-04 2003-09-02 Verbesserte verfahren zur synthese von nukleinsäuren WO2004022574A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/526,487 US20060172299A1 (en) 2002-09-04 2003-09-02 Methods for the synthesis of mucleic acids
EP03750483A EP1537236A2 (de) 2002-09-04 2003-09-02 Verbesserte verfahren zur synthese von nukleinsäuren
AU2003270143A AU2003270143A1 (en) 2002-09-04 2003-09-02 Improved methods for the synthesis of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10240868.8 2002-09-04
DE10240868A DE10240868A1 (de) 2002-09-04 2002-09-04 Verbesserte Verfahren zur Synthese von Nukleinsäuren

Publications (2)

Publication Number Publication Date
WO2004022574A2 WO2004022574A2 (de) 2004-03-18
WO2004022574A3 true WO2004022574A3 (de) 2004-04-22

Family

ID=31724332

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/009756 WO2004022574A2 (de) 2002-09-04 2003-09-02 Verbesserte verfahren zur synthese von nukleinsäuren

Country Status (5)

Country Link
US (1) US20060172299A1 (de)
EP (1) EP1537236A2 (de)
AU (1) AU2003270143A1 (de)
DE (1) DE10240868A1 (de)
WO (1) WO2004022574A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10837039B2 (en) * 2014-06-10 2020-11-17 Curevac Real Estate Gmbh Methods and means for enhancing RNA production
WO2016180430A1 (en) 2015-05-08 2016-11-17 Curevac Ag Method for producing rna
WO2016193206A1 (en) 2015-05-29 2016-12-08 Curevac Ag A method for producing and purifying rna, comprising at least one step of tangential flow filtration

Citations (4)

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WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
US6090589A (en) * 1990-12-31 2000-07-18 Promega Corporation Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US6027913A (en) * 1988-01-28 2000-02-22 Sommer; Steven S. Nucleic acid amplification with direct sequencing
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5256555A (en) * 1991-12-20 1993-10-26 Ambion, Inc. Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
WO1993018176A1 (en) * 1992-03-11 1993-09-16 Dana-Farber Cancer Institute METHODS TO CLONE mRNA
US5262311A (en) * 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA
US5514545A (en) * 1992-06-11 1996-05-07 Trustees Of The University Of Pennsylvania Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
US5780273A (en) * 1993-04-09 1998-07-14 Amoco Corporation Insertion elements and amplifiable nucleic acids
DK0632134T3 (da) * 1993-07-01 2001-12-03 Hoffmann La Roche Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
EP1251182A3 (de) * 1993-12-01 2002-11-27 Toyo Boseki Kabushiki Kaisha Verfahren zur Amplifikation und Erkennung von Nukleinsäure-Zielsequenzen unter Verwendung von thermostabilen Enzymen
JPH1028585A (ja) * 1996-07-16 1998-02-03 Toyobo Co Ltd 耐熱性リボヌクレアーゼｈを用いる核酸増幅法
US5871929A (en) * 1996-07-23 1999-02-16 Barnes; Wayne M. Suppression of pyrophosphorolysis in DNA sequencing and in other applications involving DNA replication
US5958688A (en) * 1997-04-28 1999-09-28 The Trustees Of The University Of Pennsylvania Characterization of mRNA patterns in neurites and single cells for medical diagnosis and therapeutics
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
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AU2001250858A1 (en) * 2000-03-17 2001-10-03 Gene Logic, Inc. Methods of preparing amplified nucleic acid molecules
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Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
WO1992008800A1 (en) * 1990-11-13 1992-05-29 Siska Diagnostics, Inc. Nucleic acid amplification by two-enzyme, self-sustained sequence replication
US6090589A (en) * 1990-12-31 2000-07-18 Promega Corporation Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
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PEZO V ET AL: "Hypermutagenic in vitro transcription employing biased NTP pools and manganese cations", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 186, no. 1, 20 February 1997 (1997-02-20), pages 67 - 72, XP004054881, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
DE10240868A1 (de) 2004-03-18
EP1537236A2 (de) 2005-06-08
AU2003270143A8 (en) 2004-03-29
AU2003270143A1 (en) 2004-03-29
US20060172299A1 (en) 2006-08-03
WO2004022574A2 (de) 2004-03-18

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