IL143141A0 - Thermostable enzyme promoting the fidelity of thermostable dna polymerases-for improvement of nucleic acid synthesis and amplification in vitro - Google Patents
Thermostable enzyme promoting the fidelity of thermostable dna polymerases-for improvement of nucleic acid synthesis and amplification in vitroInfo
- Publication number
- IL143141A0 IL143141A0 IL14314100A IL14314100A IL143141A0 IL 143141 A0 IL143141 A0 IL 143141A0 IL 14314100 A IL14314100 A IL 14314100A IL 14314100 A IL14314100 A IL 14314100A IL 143141 A0 IL143141 A0 IL 143141A0
- Authority
- IL
- Israel
- Prior art keywords
- thermostable
- nucleic acid
- amplification
- fidelity
- vitro
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP99119268A EP1088891B1 (de) | 1999-09-28 | 1999-09-28 | Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation |
PCT/EP2000/009423 WO2001023583A2 (en) | 1999-09-28 | 2000-09-27 | Thermostable enzyme promoting the fidelity of thermostable dna polymerases- for improvement of nucleic acid synthesis and amplification in vitro |
Publications (1)
Publication Number | Publication Date |
---|---|
IL143141A0 true IL143141A0 (en) | 2002-04-21 |
Family
ID=8239082
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL14314100A IL143141A0 (en) | 1999-09-28 | 2000-09-27 | Thermostable enzyme promoting the fidelity of thermostable dna polymerases-for improvement of nucleic acid synthesis and amplification in vitro |
Country Status (14)
Country | Link |
---|---|
US (2) | US7030220B1 (de) |
EP (2) | EP1088891B1 (de) |
JP (1) | JP3655240B2 (de) |
CN (1) | CN1185347C (de) |
AT (2) | ATE286981T1 (de) |
AU (1) | AU777229B2 (de) |
CA (1) | CA2351634C (de) |
DE (2) | DE69923180T2 (de) |
ES (1) | ES2269189T3 (de) |
IL (1) | IL143141A0 (de) |
NO (1) | NO20012561L (de) |
NZ (1) | NZ511759A (de) |
RU (1) | RU2260055C2 (de) |
WO (1) | WO2001023583A2 (de) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1088891B1 (de) | 1999-09-28 | 2005-01-12 | Roche Diagnostics GmbH | Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation |
EP1275735A1 (de) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
EP1277841B1 (de) * | 2001-07-11 | 2009-08-26 | Roche Diagnostics GmbH | Neue Zusammensetzung und Methode zur 'hot start' Nukleinsäureamplifizierung |
CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
US20030180741A1 (en) * | 2001-12-21 | 2003-09-25 | Holly Hogrefe | High fidelity DNA polymerase compositions and uses therefor |
US9181534B1 (en) | 2001-12-21 | 2015-11-10 | Agilent Technologies Inc. | High fidelity DNA polymerase compositions and uses thereof |
US7932070B2 (en) | 2001-12-21 | 2011-04-26 | Agilent Technologies, Inc. | High fidelity DNA polymerase compositions and uses therefor |
GB0208768D0 (en) | 2002-04-17 | 2002-05-29 | Univ Newcastle | DNA polymerases |
US8283148B2 (en) | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
WO2006061994A1 (ja) * | 2004-12-08 | 2006-06-15 | Takeshi Yamamoto | 遺伝子配列検査法 |
US7922177B2 (en) * | 2005-05-18 | 2011-04-12 | Diamond Game Enterprises, Inc. | Ticket strips that encourage multiple ticket purchasing |
JP2009515539A (ja) * | 2005-11-18 | 2009-04-16 | バイオライン・リミテッド | 酵素的dnaポリメラーゼ反応を増強するための方法 |
US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
US8409805B2 (en) | 2009-02-13 | 2013-04-02 | Asuragen, Inc. | Method of amplification of GC-rich DNA templates |
AU2010229334B2 (en) | 2009-03-24 | 2015-08-20 | Asuragen, Inc. | PCR methods for characterizing the 5' untranslated region of the FMR1 and FMR2 genes |
EP2419846B1 (de) * | 2009-04-16 | 2013-03-06 | Koninklijke Philips Electronics N.V. | Verfahren zur quantifizierung von nukleinsäure |
GB0915796D0 (en) | 2009-09-09 | 2009-10-07 | Fermentas Uab | Polymerase compositions and uses |
US9238832B2 (en) * | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
CA2801997C (en) | 2010-06-18 | 2016-05-31 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
CN103003419B (zh) | 2010-06-18 | 2015-07-22 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
CA2802000C (en) | 2010-06-18 | 2016-08-16 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
CN103025867B (zh) | 2010-06-18 | 2015-05-20 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
ES2536978T3 (es) | 2010-06-18 | 2015-06-01 | F. Hoffmann-La Roche Ag | ADN polimerasas con discriminación incrementada de no correspondencias 3' |
EP2582806B1 (de) | 2010-06-18 | 2015-12-16 | Roche Diagnostics GmbH | Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz |
EP2582804B1 (de) | 2010-06-18 | 2015-02-25 | Roche Diagnostics GmbH | Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen |
CA2801998C (en) | 2010-06-18 | 2016-11-08 | F. Hoffmann La-Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
WO2012110061A1 (en) | 2011-02-15 | 2012-08-23 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
CN103492559B (zh) | 2011-04-11 | 2016-07-06 | 霍夫曼-拉罗奇有限公司 | 具有改进活性的dna聚合酶 |
CA2839964C (en) | 2011-07-28 | 2019-03-12 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
EP2559774A1 (de) * | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes |
CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
EP2788479B1 (de) | 2011-12-08 | 2016-02-10 | Roche Diagnostics GmbH | Dna-polymerasen mit verbesserter aktivität |
ES2716277T3 (es) | 2011-12-08 | 2019-06-11 | Hoffmann La Roche | ADN polimerasas con actividad mejorada |
US9371560B2 (en) | 2012-07-20 | 2016-06-21 | Asuragen, Inc. | Comprehensive FMR1 genotyping |
GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
EP3850086A1 (de) | 2018-09-13 | 2021-07-21 | F. Hoffmann-La Roche AG | Mutante dna-polymerase(n) mit verbesserter strangverschiebbarkeit |
WO2022071888A1 (en) * | 2020-10-02 | 2022-04-07 | National University Of Singapore | A dna assembly mix and method of uses thereof |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990012115A1 (en) * | 1989-03-21 | 1990-10-18 | Collaborative Research, Inc. | A dna diagnostic test using an exonuclease activity |
US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
WO1994023066A1 (en) | 1993-03-30 | 1994-10-13 | United States Biochemical Corporation | Use of exonuclease in dna sequencing |
US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
CN1137266C (zh) * | 1995-12-27 | 2004-02-04 | 宝酒造株式会社 | 新型dna聚合酶 |
WO1998045452A2 (en) * | 1997-04-08 | 1998-10-15 | The Rockfeller University | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
US6238905B1 (en) * | 1997-09-12 | 2001-05-29 | University Technology Corporation | Thermophilic polymerase III holoenzyme |
DE19810879A1 (de) | 1998-03-13 | 1999-09-16 | Roche Diagnostics Gmbh | Polymerasenchimären |
DE19813317A1 (de) * | 1998-03-26 | 1999-09-30 | Roche Diagnostics Gmbh | Verbessertes Verfahren zur Primer Extension Präamplifikations-PCR |
AU5008900A (en) * | 1999-05-12 | 2000-11-21 | Invitrogen Corporation | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
EP1088891B1 (de) * | 1999-09-28 | 2005-01-12 | Roche Diagnostics GmbH | Thermostabiles Enzym welches die Genauigkeit thermostabiler DNA Polymerasen erhöht - zur Verbesserung der Nucleinsäuresynthese und in vitro Amplifikation |
EP1275735A1 (de) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
-
1999
- 1999-09-28 EP EP99119268A patent/EP1088891B1/de not_active Expired - Lifetime
- 1999-09-28 DE DE69923180T patent/DE69923180T2/de not_active Expired - Fee Related
- 1999-09-28 AT AT99119268T patent/ATE286981T1/de not_active IP Right Cessation
-
2000
- 2000-09-27 IL IL14314100A patent/IL143141A0/xx unknown
- 2000-09-27 AU AU79077/00A patent/AU777229B2/en not_active Ceased
- 2000-09-27 EP EP00969312A patent/EP1144653B1/de not_active Expired - Lifetime
- 2000-09-27 DE DE60029927T patent/DE60029927T2/de not_active Expired - Lifetime
- 2000-09-27 US US09/856,850 patent/US7030220B1/en not_active Expired - Lifetime
- 2000-09-27 AT AT00969312T patent/ATE335825T1/de not_active IP Right Cessation
- 2000-09-27 RU RU2001117496/13A patent/RU2260055C2/ru not_active IP Right Cessation
- 2000-09-27 NZ NZ511759A patent/NZ511759A/en not_active IP Right Cessation
- 2000-09-27 ES ES00969312T patent/ES2269189T3/es not_active Expired - Lifetime
- 2000-09-27 JP JP2001526965A patent/JP3655240B2/ja not_active Expired - Lifetime
- 2000-09-27 CN CNB008030588A patent/CN1185347C/zh not_active Expired - Lifetime
- 2000-09-27 WO PCT/EP2000/009423 patent/WO2001023583A2/en active IP Right Grant
- 2000-09-27 CA CA002351634A patent/CA2351634C/en not_active Expired - Lifetime
-
2001
- 2001-05-25 NO NO20012561A patent/NO20012561L/no not_active Application Discontinuation
-
2005
- 2005-09-29 US US11/241,116 patent/US7410782B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US7030220B1 (en) | 2006-04-18 |
EP1088891B1 (de) | 2005-01-12 |
DE60029927T2 (de) | 2007-03-08 |
CN1343254A (zh) | 2002-04-03 |
DE69923180T2 (de) | 2006-01-12 |
JP2003510084A (ja) | 2003-03-18 |
DE69923180D1 (de) | 2005-02-17 |
CA2351634A1 (en) | 2001-04-05 |
JP3655240B2 (ja) | 2005-06-02 |
DE60029927D1 (de) | 2006-09-21 |
EP1088891A1 (de) | 2001-04-04 |
CN1185347C (zh) | 2005-01-19 |
CA2351634C (en) | 2007-02-20 |
EP1144653A2 (de) | 2001-10-17 |
WO2001023583A2 (en) | 2001-04-05 |
US7410782B2 (en) | 2008-08-12 |
WO2001023583A3 (en) | 2001-10-25 |
ATE286981T1 (de) | 2005-01-15 |
AU777229B2 (en) | 2004-10-07 |
NO20012561D0 (no) | 2001-05-25 |
ES2269189T3 (es) | 2007-04-01 |
EP1144653B1 (de) | 2006-08-09 |
NZ511759A (en) | 2003-12-19 |
US20060078928A1 (en) | 2006-04-13 |
ATE335825T1 (de) | 2006-09-15 |
NO20012561L (no) | 2001-07-18 |
RU2260055C2 (ru) | 2005-09-10 |
AU7907700A (en) | 2001-04-30 |
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