ATE309336T1 - Umkehrbare modifikation von thermostabilen enzymen - Google Patents
Umkehrbare modifikation von thermostabilen enzymenInfo
- Publication number
- ATE309336T1 ATE309336T1 AT99110426T AT99110426T ATE309336T1 AT E309336 T1 ATE309336 T1 AT E309336T1 AT 99110426 T AT99110426 T AT 99110426T AT 99110426 T AT99110426 T AT 99110426T AT E309336 T1 ATE309336 T1 AT E309336T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- thermostable enzymes
- enzyme
- reversible modification
- amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8684698A | 1998-05-29 | 1998-05-29 | |
US09/183,950 US6183998B1 (en) | 1998-05-29 | 1998-10-31 | Method for reversible modification of thermostable enzymes |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE309336T1 true ATE309336T1 (de) | 2005-11-15 |
Family
ID=26775213
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT99110426T ATE309336T1 (de) | 1998-05-29 | 1999-05-28 | Umkehrbare modifikation von thermostabilen enzymen |
Country Status (6)
Country | Link |
---|---|
US (1) | US6183998B1 (de) |
EP (1) | EP0962526B1 (de) |
JP (1) | JP4398004B2 (de) |
AT (1) | ATE309336T1 (de) |
DE (1) | DE69928169T2 (de) |
ES (1) | ES2253848T3 (de) |
Families Citing this family (68)
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US6828094B2 (en) * | 1996-12-20 | 2004-12-07 | Roche Diagnostics Gmbh | Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application |
JP2002543795A (ja) * | 1999-05-12 | 2002-12-24 | インビトロゲン・コーポレーション | 核酸合成の感度および特異性の増大のための組成物および方法 |
GB9920194D0 (en) * | 1999-08-27 | 1999-10-27 | Advanced Biotech Ltd | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
US9771565B2 (en) | 2000-05-26 | 2017-09-26 | Life Technologies Corporation | Thermostable reverse transcriptases and uses thereof |
US7078208B2 (en) * | 2000-05-26 | 2006-07-18 | Invitrogen Corporation | Thermostable reverse transcriptases and uses thereof |
JP2001352982A (ja) * | 2000-06-12 | 2001-12-25 | Shimadzu Corp | 核酸合成法 |
GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
US6667165B2 (en) | 2001-11-13 | 2003-12-23 | Eppendorf Ag | Method and compositions for reversible inhibition of thermostable polymerases |
US7772383B2 (en) * | 2002-01-25 | 2010-08-10 | The Trustees Of Princeton University | Chemical PCR: Compositions for enhancing polynucleotide amplification reactions |
US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
EP2325303B1 (de) | 2002-09-13 | 2013-11-06 | Life Technologies Corporation | Wärmestabile reverse Transkriptasen und Verwendungen davon |
EP1411133B1 (de) * | 2002-09-24 | 2009-01-07 | Qiagen GmbH | Verbesserte Koamplifikation von Nukleinsäuren |
EP1403379A1 (de) * | 2002-09-24 | 2004-03-31 | QIAGEN GmbH | Verbesserte Koamplifikation von Nukleinsäuren |
CA2519309A1 (en) | 2003-03-25 | 2004-10-14 | Stratagene California | Dna polymerase fusions and uses thereof |
DE10315640A1 (de) * | 2003-04-04 | 2004-10-14 | Ignatov, Konstantin | Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung |
ATE418621T1 (de) * | 2003-04-24 | 2009-01-15 | Afshin Ahmadian | Verfahren zur detektion allelspezifischer mutationen |
AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
US20060166363A1 (en) * | 2004-01-27 | 2006-07-27 | Sergei Zolotukhin | Modified baculovirus expression system for production of pseudotyped rAAV vector |
EP2292767B1 (de) * | 2004-06-04 | 2013-11-20 | Takara Bio, Inc. | Polypeptide mit DNA-Polymeraseaktivität |
US20060019366A1 (en) * | 2004-06-09 | 2006-01-26 | Wanli Bi | Reversibly modified thermostable enzyme compositions and methods of making and using the same |
US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
EP2208796B1 (de) | 2005-01-06 | 2014-06-18 | Applied Biosystems, LLC | Polypeptide mit Nukleinsäurebindungsaktivität und Zusammensetzungen und Verfahren zur Nukleinsäurenamplifikation |
WO2006076650A2 (en) * | 2005-01-12 | 2006-07-20 | Applera Corporation | Compositions, methods, and kits for selective amplification of nucleic acids |
JP2008541761A (ja) * | 2005-05-31 | 2008-11-27 | インヴィトロジェン コーポレーション | パラフィン含有試料からの核酸の分離及び精製 |
AU2006287548A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
US20070059713A1 (en) | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
DE102005047617A1 (de) | 2005-10-05 | 2007-04-19 | Qiagen Gmbh | Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft |
GB0600228D0 (en) | 2006-01-06 | 2006-02-15 | Fermentas Uab | Inactivation method |
DE102006015960A1 (de) * | 2006-04-04 | 2007-10-11 | Qiagen Gmbh | Gemisch reversibel inhibierter Enzyme |
US8435774B2 (en) * | 2006-06-28 | 2013-05-07 | Qiagen Gmbh | Enhancing reactivation of thermostable reversibly inactivated enzymes |
US9045522B2 (en) | 2006-07-31 | 2015-06-02 | Wanli Bi | Nucleic acid amplification using a reversibly modified oligonucleotide |
ATE538216T1 (de) * | 2006-07-31 | 2012-01-15 | Wanli Bi | Nukleinsäureverstärkung mithilfe eines reversibel modifizierten oligonukleotids |
EP2140001A2 (de) * | 2007-04-25 | 2010-01-06 | 3M Innovative Properties Company | Verfahren zur amplifikation von nukleinsäuren |
EP2183380B1 (de) * | 2007-08-27 | 2014-11-26 | Life Technologies Corporation | Verfahren und zusammensetzungen für pcr |
DE102008047790A1 (de) | 2008-09-17 | 2010-04-15 | Qiagen Gmbh | Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe |
EP2411539B1 (de) | 2009-03-25 | 2018-10-03 | Life Technologies Corporation | Diskriminatorische dna-positiv- bzw. -extraktionskontrolle |
WO2011044444A2 (en) * | 2009-10-09 | 2011-04-14 | Life Technologies Corporation | cDNA SYNTHESIS USING A REVERSIBLY INACTIVATED REVERSE TRANSCRIPTASE |
IN2012DN05145A (de) | 2009-11-19 | 2015-10-23 | Solis Biodyne | |
US8618253B2 (en) * | 2010-05-25 | 2013-12-31 | Samsung Techwin Co., Ltd. | Modified RNAse H and detection of nucleic acid amplification |
CN105378109B (zh) | 2013-03-14 | 2019-06-14 | 雅培分子公司 | 使用尿嘧啶-dna-n-糖基化酶使错误最小化 |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
KR101488110B1 (ko) | 2013-11-29 | 2015-01-29 | 성균관대학교산학협력단 | 나노아케움 이퀴탄스 유래의 Neq HS DNA 중합효소의 돌연변이체 제조 및 이를 이용한 hot-start PCR 응용 |
EP3097190A2 (de) | 2014-01-22 | 2016-11-30 | Life Technologies Corporation | Neuartige reverse transkriptasen zur verwendung bei hochtemperatur-nukleinsäuresynthese |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
ES2759477T3 (es) | 2015-05-29 | 2020-05-11 | Hoffmann La Roche | Un procedimiento de inactivación de microbios por anhídrido citracónico |
EP3365357B1 (de) | 2015-10-23 | 2024-02-14 | President and Fellows of Harvard College | Entwickelte cas9-proteine für geneditierung |
CN116144626A (zh) | 2016-01-13 | 2023-05-23 | 新英格兰生物实验室公司 | T7 rna聚合酶的热稳定变体 |
EP3464596A1 (de) | 2016-06-07 | 2019-04-10 | Cepheid | Thermostabile polymeraseinhibitorzusammensetzungen und verfahren |
AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
IL306092A (en) | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
CN111229023B (zh) * | 2018-11-29 | 2022-01-28 | 内蒙古东盛硅藻土科技创新产业园有限公司 | 一种化学降解添加剂 |
JP2022526908A (ja) | 2019-03-19 | 2022-05-27 | ザ ブロード インスティテュート,インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
JP2023543803A (ja) | 2020-09-24 | 2023-10-18 | ザ ブロード インスティテュート,インコーポレーテッド | プライム編集ガイドrna、その組成物、及びその使用方法 |
JP2024503437A (ja) | 2021-01-11 | 2024-01-25 | ザ ブロード インスティテュート,インコーポレーテッド | プライム編集効率及び精度を向上させるためのプライム編集因子バリアント、構築物、及び方法 |
WO2023076898A1 (en) | 2021-10-25 | 2023-05-04 | The Broad Institute, Inc. | Methods and compositions for editing a genome with prime editing and a recombinase |
CN116064468B (zh) * | 2022-11-04 | 2023-11-21 | 南京诺唯赞生物科技股份有限公司 | 一种化学修饰Taq酶储存液 |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
KR100204837B1 (ko) * | 1989-04-28 | 1999-06-15 | 기스트 브로카데스 나암로제 베노오트하프 | 카르복실 에스테라제의 안정화 |
ATE188509T1 (de) | 1990-02-16 | 2000-01-15 | Hoffmann La Roche | Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion |
US5338671A (en) | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
KR960703174A (ko) * | 1993-06-09 | 1996-06-19 | 미안 알렉 | 자기장 순환식 반응방법(magnetic cycle reaction) |
AU694479B2 (en) * | 1994-01-31 | 1998-07-23 | Regents Of The University Of California, The | Methods for the elimination of DNA sequencing artifacts |
US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
-
1998
- 1998-10-31 US US09/183,950 patent/US6183998B1/en not_active Expired - Lifetime
-
1999
- 1999-05-27 JP JP14761899A patent/JP4398004B2/ja not_active Expired - Lifetime
- 1999-05-28 AT AT99110426T patent/ATE309336T1/de active
- 1999-05-28 ES ES99110426T patent/ES2253848T3/es not_active Expired - Lifetime
- 1999-05-28 DE DE69928169T patent/DE69928169T2/de not_active Expired - Lifetime
- 1999-05-28 EP EP99110426A patent/EP0962526B1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69928169T2 (de) | 2006-08-03 |
US6183998B1 (en) | 2001-02-06 |
ES2253848T3 (es) | 2006-06-01 |
EP0962526B1 (de) | 2005-11-09 |
DE69928169D1 (de) | 2005-12-15 |
JP2000004878A (ja) | 2000-01-11 |
JP4398004B2 (ja) | 2010-01-13 |
EP0962526A3 (de) | 2002-01-16 |
EP0962526A2 (de) | 1999-12-08 |
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