ATE188509T1 - Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion - Google Patents

Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion

Info

Publication number
ATE188509T1
ATE188509T1 AT91904707T AT91904707T ATE188509T1 AT E188509 T1 ATE188509 T1 AT E188509T1 AT 91904707 T AT91904707 T AT 91904707T AT 91904707 T AT91904707 T AT 91904707T AT E188509 T1 ATE188509 T1 AT E188509T1
Authority
AT
Austria
Prior art keywords
pcr
polymerase chain
chain reaction
enzymatic
pcr reagents
Prior art date
Application number
AT91904707T
Other languages
English (en)
Inventor
Will Bloch
Jonathan Raymond
Alan R Read
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE188509T1 publication Critical patent/ATE188509T1/de

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/10—Surface shaping of articles, e.g. embossing; Apparatus therefor by electric discharge treatment
    • B29C59/103—Surface shaping of articles, e.g. embossing; Apparatus therefor by electric discharge treatment of profiled articles, e.g. hollow or tubular articles
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/0427—Coating with only one layer of a composition containing a polymer binder
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/046—Forming abrasion-resistant coatings; Forming surface-hardening coatings
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2471/00—Characterised by the use of polyethers obtained by reactions forming an ether link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G01N2035/00247—Microvalves
    • G01N2035/00267—Meltable plugs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT91904707T 1990-02-16 1991-02-15 Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion ATE188509T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48150190A 1990-02-16 1990-02-16
PCT/US1991/001039 WO1991012342A1 (en) 1990-02-16 1991-02-15 Improvements in the specificity and convenience of the polymerase chain reaction

Publications (1)

Publication Number Publication Date
ATE188509T1 true ATE188509T1 (de) 2000-01-15

Family

ID=23912174

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91904707T ATE188509T1 (de) 1990-02-16 1991-02-15 Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion

Country Status (10)

Country Link
US (1) US5411876A (de)
EP (1) EP0515506B1 (de)
JP (2) JP3087907B2 (de)
AT (1) ATE188509T1 (de)
AU (1) AU653712B2 (de)
CA (1) CA2075050C (de)
DE (1) DE69131891T2 (de)
DK (1) DK0515506T3 (de)
ES (1) ES2141088T3 (de)
WO (1) WO1991012342A1 (de)

Families Citing this family (171)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
WO1993015222A1 (fr) * 1992-01-29 1993-08-05 Institut National De La Sante Et De La Recherche Medicale (Inserm) Procede pour eviter les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
EP0572057A1 (de) * 1992-05-11 1993-12-01 Johnson & Johnson Clinical Diagnostics, Inc. PCR-Reagenzien- und Testsatz und Verfahren für die Amplifikation und Detektion mit verringerter, nicht-spezifischer Amplifikation von Nukleinsäuren
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5599660A (en) * 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
AU6029694A (en) * 1993-01-19 1994-08-15 Pharmacia P-L Biochemicals, Inc. Storage and delivery of purified protein reagents with carrier wax
WO1995011083A2 (en) * 1993-10-22 1995-04-27 Abbott Laboratories Reaction tube and method of use to minimize contamination
WO1995011315A1 (en) * 1993-10-22 1995-04-27 Invitrogen Corporation Packaging of reaction components in wax for release under specified temperature conditions
WO1996000301A1 (en) * 1994-06-24 1996-01-04 The University Of Houston Encapsulated pcr reagents
ZA956776B (en) * 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5576197A (en) * 1995-04-07 1996-11-19 Molecular Bio-Products Polymerase chain reaction container and methods of using the same
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US5721123A (en) * 1996-01-05 1998-02-24 Microfab Technology, Inc. Methods and apparatus for direct heating of biological material
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
JP2000516468A (ja) 1996-08-14 2000-12-12 ライフ テクノロジーズ，インコーポレイテッド 核酸増幅および配列決定のための安定な組成物
JP3182527B2 (ja) * 1996-09-03 2001-07-03 株式会社ヤトロン 化学発光の測定方法及びキット
US6110710A (en) * 1996-10-15 2000-08-29 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition
EP1801214B1 (de) * 1997-07-07 2010-11-10 Medical Research Council In-vitro-Sortierverfahren
US6893877B2 (en) * 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
ATE369915T1 (de) * 1998-05-01 2007-09-15 Gen Probe Inc Inkubator für automatische analysevorrichtung
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US20020055118A1 (en) * 1998-06-24 2002-05-09 Yong-Bin Eym Method of preparing objects containing DNA
ES2153745B1 (es) * 1998-07-31 2001-11-16 Ivia Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado.
US6413780B1 (en) * 1998-10-14 2002-07-02 Abbott Laboratories Structure and method for performing a determination of an item of interest in a sample
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
EP1165235B1 (de) * 1999-03-19 2011-09-28 Life Technologies Corporation Methode zum sichten von mutierten zellen
ES2228520T3 (es) 1999-05-04 2005-04-16 Ortho-Clinical Diagnostics, Inc. Capatura rapida y eficaz de adn de una muestra sin usar reactivo de lisis celular.
US6643787B1 (en) 1999-10-19 2003-11-04 Rambus Inc. Bus system optimization
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
WO2002012263A1 (en) * 2000-08-03 2002-02-14 Roche Diagnostics Gmbh Nucleic acid binding compounds containing pyrazolo[3,4-d]pyrimidine analogues of purin-2,6-diamine and their uses
US20070037144A1 (en) * 2000-10-20 2007-02-15 Jay Wohlgemuth Leukocyte expression profiling
WO2002034905A1 (fr) * 2000-10-27 2002-05-02 Supercritical Co., Ltd. Procédé d'amplification de gènes
FR2817961B1 (fr) * 2000-12-08 2003-08-01 Diagast Barriere de separation temporaire, recipient la comprenant et procede de mise en oeuvre d'un test dans ce recipient
US6428962B1 (en) 2001-02-12 2002-08-06 Dna Analysis, Inc. Nucleic acid collection barrier method and apparatus
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
AU2002311183B2 (en) * 2001-06-12 2007-03-01 Takara Bio Inc. Method of stabilizing reagent for amplifying or detecting nucleic acid and storage method
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
WO2004011666A2 (en) * 2002-07-26 2004-02-05 Applera Corporation Mg-mediated hot start biochemical reactions
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP1403379A1 (de) * 2002-09-24 2004-03-31 QIAGEN GmbH Verbesserte Koamplifikation von Nukleinsäuren
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
CA2513889A1 (en) * 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2004101733A1 (fr) * 2003-05-01 2004-11-25 Southeast University Dispositif d'amplification pcr pour reaction a etapes multiples et tube d'amplification pcr sans lavage pour detection de genes
PT1526187E (pt) 2003-05-01 2008-10-16 Veridex Llc Ensaios de gânglios linfáticos intra-operatórios
US20060003343A1 (en) * 2004-01-23 2006-01-05 Biomerieux, Inc. Primer and probe design for efficient amplification and detection of HCV 3' non-translating region
EP1559478B1 (de) * 2004-01-23 2010-06-16 Qiagen GmbH Kontaminationsbarriere
EP1735097B1 (de) 2004-03-12 2016-11-30 Life Technologies Corporation Ladung eines nanoliter-arrays
DE102004021822B3 (de) * 2004-04-30 2005-11-17 Siemens Ag Verfahren und Anordnung zur DNA-Amplifikation mittels PCR unter Einsatz von Trockenreagenzien
MXPA06014175A (es) * 2004-06-04 2007-05-09 Johnson & Johnson Diagnostico o pronostico del curso del cancer de mama.
US20060019366A1 (en) * 2004-06-09 2006-01-26 Wanli Bi Reversibly modified thermostable enzyme compositions and methods of making and using the same
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
US20060029801A1 (en) * 2004-08-03 2006-02-09 O'brien Robert Method for making a coated powder for reducing evaporative water loss
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
JP5165383B2 (ja) * 2004-12-23 2013-03-21 アイ−スタツト・コーポレイシヨン 分子診断システム及び方法
WO2006106867A1 (ja) * 2005-03-30 2006-10-12 Shimadzu Corporation 遺伝子多型診断用装置
EP1885889A4 (de) * 2005-05-11 2010-01-20 Expression Diagnostics Inc Verfahren zur überwachung des betriebsstatus von transplantaten über genlisten
US20070003443A1 (en) * 2005-06-23 2007-01-04 Applera Corporation Thermal-cycling pipette tip
EP2308990B1 (de) 2005-07-15 2012-09-26 Life Technologies Corporation Analyse von Boten-RNA und Mikro-RNA im gleichen Reaktionsansatz
WO2007011901A2 (en) 2005-07-15 2007-01-25 Applera Corporation Hot start reverse transcription by primer design
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
GB0600228D0 (en) 2006-01-06 2006-02-15 Fermentas Uab Inactivation method
ES2364983T3 (es) * 2006-02-27 2011-09-20 F. Hoffmann-La Roche Ag Reacción en cadena de polimerasa con arranque en caliente por secuestro de magnesio.
EP2019687B1 (de) 2006-03-29 2014-03-19 Merial Limited Impfstoff gegen streptokokken
WO2008051290A2 (en) * 2006-04-07 2008-05-02 Xdx, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
BRPI0715427B1 (pt) * 2006-07-31 2016-04-12 Wanli Bi método para amplificação de sequência ou sinal de ácidos nucleicos alvo, oligonucleotideo modificado reversível, mistura e conjunto de reação de amplificação de ácido nucleico
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) * 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
WO2008065996A1 (fr) * 2006-11-28 2008-06-05 Shimadzu Corporation Plaque de réaction
JP4307478B2 (ja) * 2006-12-05 2009-08-05 キヤノン株式会社 生化学反応用カートリッジ
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
WO2008122843A2 (en) * 2007-04-10 2008-10-16 Pontes Luis Andre Use of liquid barriers to perform hot start pcr
WO2008134470A2 (en) * 2007-04-25 2008-11-06 3M Innovative Properties Company Methods for nucleic acid amplification
WO2008146649A1 (ja) * 2007-05-25 2008-12-04 Olympus Corporation 酵素含有ゲル及び核酸の増幅キット
JPWO2008146686A1 (ja) * 2007-05-25 2010-08-19 ベックマン・コールター・インコーポレーテッド 酵素含有カプセル及び核酸の増幅キット
WO2009029648A2 (en) 2007-08-27 2009-03-05 Applera Corporation Methods and compositions for pcr
WO2009054473A1 (ja) * 2007-10-26 2009-04-30 Toppan Printing Co., Ltd. 反応チップ及び反応方法、遺伝子処理装置用温度調節機構及び遺伝子処理装置
CA2658520C (en) * 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
EP2279263B1 (de) 2008-04-30 2013-09-04 Integrated Dna Technologies, Inc. Tests auf rnase-h-basis mit modifizierten rna-monomeren
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2990487A1 (de) 2008-05-08 2016-03-02 Asuragen, INC. Zusammensetzungen und verfahren in zusammenhang mit der mirna-modulation von neovaskularisation oder angiogenese
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
IT1397110B1 (it) * 2008-12-29 2012-12-28 St Microelectronics Rousset Microreattore autosigillante e metodo per eseguire una reazione
JP5608997B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
JP5608998B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
WO2010117456A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc Column enrichment of pcr beads comprising tethered amplicons
US8597892B2 (en) 2009-05-22 2013-12-03 Asuragen, Inc. MiRNA biomarkers of prostate disease
IL206810A (en) 2009-07-08 2015-07-30 Janssen Diagnostics Llc Methods for identifying melanoma and kits for using these methods
JP5229160B2 (ja) * 2009-08-26 2013-07-03 株式会社島津製作所 反応容器
CN102725632A (zh) 2009-08-28 2012-10-10 奥斯瑞根公司 肺病的miRNA生物标志物
WO2011060014A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
CA2977845C (en) 2010-02-23 2020-08-04 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
SI23321A (sl) 2010-03-03 2011-09-30 Inštitut za fizikalno biologijo d.o.o. Izboljĺ ano zbiranje fluorescentne svetlobe iz vzorca med pomnoĺ˝evanjem v rt-pcr
DK201000356A (en) * 2010-04-22 2011-10-23 Statens Seruminstitut New nucleotide sequence amplification Method
JP5454338B2 (ja) * 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
EP2586861B1 (de) * 2010-06-22 2017-10-25 Universal Bio Research Co., Ltd. Zusammensetzung zur verhinderung der verdampfung einer reaktionslösung während einer nukleinsäure-amplifikationsreaktion
CN103675303B (zh) 2010-07-23 2016-02-03 贝克曼考尔特公司 传感器系统
WO2012024103A2 (en) * 2010-08-17 2012-02-23 Rheonix, Inc. Thermocycler seal composition, method, and application
WO2012068400A2 (en) 2010-11-17 2012-05-24 Asuragen, Inc. Mirnas as biomarkers for distinguishing benign from malignant thyroid neoplasms
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US9469871B2 (en) 2011-04-14 2016-10-18 Corporos Inc. Methods and apparatus for point-of-care nucleic acid amplification and detection
US8911941B2 (en) 2011-04-14 2014-12-16 Kenneth J. Michlitsch Methods and apparatus for point-of-care nucleic acid amplification and detection
EP2705130B1 (de) 2011-05-04 2016-07-06 Luminex Corporation Vorrichtung und verfahren für integrierte probenzubereitung, -reaktion und -erkennung
WO2012155097A2 (en) * 2011-05-11 2012-11-15 Luminex Corporation Systems and methods for producing an evaporation barrier in a reaction chamber
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US8475739B2 (en) * 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
JP2014534949A (ja) 2011-09-19 2014-12-25 ジェネンテック， インコーポレイテッド Ｃ−ｍｅｔアンタゴニストおよびｂ−ｒａｆアンタゴニストを含む組合せ処置
EP2758546B1 (de) 2011-09-23 2017-11-22 Roche Diagnostics GmbH Verwendung von g-clamp für verbesserte allelspezifische pcr
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
GB2501238B (en) 2012-03-16 2018-10-24 Cambridge Entpr Ltd Methods for obtaining liquid from a solid phase
US9354159B2 (en) 2012-05-02 2016-05-31 Nanoscopia (Cayman), Inc. Opto-fluidic system with coated fluid channels
US9085761B1 (en) 2012-06-14 2015-07-21 Affymetrix, Inc. Methods and compositions for amplification of nucleic acids
DE102012222351A1 (de) 2012-12-05 2014-06-05 Gna Biosolutions Gmbh Reaktionsgefäß mit magnetischem Verschluss
WO2014145612A1 (en) 2013-03-15 2014-09-18 Ajay Goel Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
CA2905949A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
US11545241B1 (en) 2013-09-07 2023-01-03 Labrador Diagnostics Llc Systems and methods for analyte testing and data management
US20150191794A1 (en) 2014-01-05 2015-07-09 Biomirna Holdings Ltd. Lung Cancer Determinations Using MIRNA
US10619210B2 (en) 2014-02-07 2020-04-14 The Johns Hopkins University Predicting response to epigenetic drug therapy
US9937494B2 (en) 2014-06-16 2018-04-10 Life Technologies Corporation Reagent mixer and fluid control devices
KR102448151B1 (ko) 2014-06-17 2022-09-30 라이프 테크놀로지스 코포레이션 서열분석 디바이스
CN107148479A (zh) 2014-09-04 2017-09-08 赛拉诺斯股份有限公司 病原体和抗微生物剂抗性检测
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
EP3286338B1 (de) 2015-04-24 2023-11-01 Atila Biosystems Incorporated Amplifikation mit primern einer begrenzten nukleotidzusammensetzung
EP3292214A1 (de) 2015-05-04 2018-03-14 Academisch Medisch Centrum Verfahren zur quantifizierung von mirnas mittels normalisierung
EP3303548A4 (de) 2015-06-05 2019-01-02 Miroculus Inc. Verdampfungsverwaltung in digitalen mikrofluidischen vorrichtungen
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
EP3345005B1 (de) * 2015-08-31 2023-11-15 Siemens Healthcare Diagnostics Inc. Verfahren und vorrichtung zur verhinderung des spritzens einer unverschlossenen flüssigkeitsprobe während des transports auf einer labordiagnoseausrüstung
EP4286529A3 (de) * 2016-02-05 2024-03-27 Gen-Probe Incorporated Getrocknete verstärkungszusammensetzungen
WO2017223026A1 (en) * 2016-06-20 2017-12-28 Miroculus Inc. Detection of rna using ligation actuated loop mediated amplification methods and digital microfluidics
US10596572B2 (en) 2016-08-22 2020-03-24 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
CA3049416A1 (en) 2016-12-28 2018-07-05 Miroculus Inc. Digital microfluidic devices and methods
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
US20180320218A1 (en) * 2017-05-08 2018-11-08 Smobio Technology, Inc. Kit for polymerase chain reaction
CN110892258A (zh) 2017-07-24 2020-03-17 米罗库鲁斯公司 具有集成的血浆收集设备的数字微流控系统和方法
EP3676009A4 (de) 2017-09-01 2021-06-16 Miroculus Inc. Digitale mikrofluidikvorrichtungen und verfahren zu ihrer verwendung
EP3719144A1 (de) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung
US11738345B2 (en) 2019-04-08 2023-08-29 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
EP3999664A1 (de) 2019-07-19 2022-05-25 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Verfahren zur bestimmung des ansprechens auf die behandlung eines patienten mit nicht-kleinzelligem lungenkarzinom (nsclc)
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CN113373202A (zh) * 2020-03-10 2021-09-10 苏州先达基因科技有限公司 防污染单管核酸恒温扩增检测系统
CN114657236A (zh) * 2020-12-23 2022-06-24 广州达安基因股份有限公司 一种分隔核酸扩增体系的方法和分隔剂
CN113680406B (zh) * 2021-08-26 2022-04-15 清华大学 一种微流控芯片多指标检测方法
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE100617C (de) *
US3919085A (en) * 1974-02-27 1975-11-11 Becton Dickinson Co Plasma separator assembly
US4032492A (en) * 1976-02-25 1977-06-28 H. B. Fuller Company Hot melt container closure sealant
AU520409B2 (en) * 1977-05-25 1982-01-28 Commonwealth Scientific And Industrial Research Organisation Controlled release composition
US4522786A (en) * 1983-08-10 1985-06-11 E. I. Du Pont De Nemours And Company Multilayered test device for detecting analytes in liquid test samples
DE3416094A1 (de) * 1984-04-30 1985-10-31 Hilti Ag, Schaan Zerstoerbare patrone fuer mehrkomponenten-massen
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1988006634A1 (en) * 1987-02-26 1988-09-07 The University Of Sydney A method of detection of carcinogenic human papillomavirus
CA1338505C (en) * 1989-02-03 1996-08-06 John Bruce Findlay Containment cuvette for pcr and method of use

Also Published As

Publication number Publication date
AU653712B2 (en) 1994-10-13
JP3532826B2 (ja) 2004-05-31
WO1991012342A1 (en) 1991-08-22
JP2000316561A (ja) 2000-11-21
ES2141088T3 (es) 2000-03-16
AU7307391A (en) 1991-09-03
JPH05506143A (ja) 1993-09-16
EP0515506A1 (de) 1992-12-02
US5411876A (en) 1995-05-02
EP0515506B1 (de) 2000-01-05
DE69131891T2 (de) 2000-06-15
JP3087907B2 (ja) 2000-09-18
DK0515506T3 (da) 2000-05-08
CA2075050A1 (en) 1991-08-17
DE69131891D1 (de) 2000-02-10
CA2075050C (en) 1998-10-06

Similar Documents

Publication Publication Date Title
ATE188509T1 (de) Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
Fakruddin et al. Nucleic acid amplification: Alternative methods of polymerase chain reaction
Thompson et al. Developmental validation of the PowerPlex® Y23 System: a single multiplex Y-STR analysis system for casework and database samples
Lowery et al. Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesis termination factor p. II. Influence of synthetic RNA homopolymers and random copolymers on the reaction.
Kox et al. A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
ATE105870T1 (de) Replikative rns-rapporteur-systeme.
KR930700681A (ko) 생체외 증폭을 이용한 폴리뉴클레오티드 검체분석
DK0726324T3 (da) Anvendelse af exonuklease og/eller glycosylase som supplementer til antipolymeraseantistof til forøgelse af specification i polymerasekædereaktion
KR900016473A (ko) 진단 키트, 프라이머 조성물 및 이것을 이용해 핵산을 복제하거나 검사하는 방법
MX9701479A (es) Metodo de marcado con lucifrasa.
Liu et al. Characterization of RNA primer synthesis in the T4 bacteriophage in vitro DNA replication system.
EP0875582A3 (de) Verfahren zur Typisierung von HLA-Klass-1-DNS mittels PCR-Amplifizierung
Sun et al. Helicase promotes replication re-initiation from an RNA transcript
KR20130081187A (ko) 표적 핵산의 향상된 증폭
MXPA97005364A (es) Amplificacion por desplazamiento de hebra usando nucleotidos boronados
EP0826779A1 (de) Strangverdrägungsamplifikation unter Verwendung von Bor enthaltenden Nukleotiden
Tews et al. Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants
Bader et al. Analysis of chimerism after stem cell transplantation
JP2013538587A (ja) Ｐｃｒ反応緩衝液中での細胞溶解のための方法
Hernandez et al. Kinetics of lagging-strand DNA synthesis in vitro by the bacteriophage T7 replication proteins
Gussoni et al. Evaluating human T cell receptor gene expression by PCR
Sandalli et al. Comparison of DIG-11-dUTP utilization by Geobacillus caldoxylosilyticus TK4, Mycobacterium tuberculosis and Escherichia coli DNA polymerases
Johnson et al. A fluorescent based PCR assay for the detection and quantitation of Giardia duodenalis genotypes in mixed populations
Sachadyn et al. Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers.
Nelson et al. Multiplexed, lateral flow, polymerase chain reaction (PCR) techniques for biological identification

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time