MX9701479A - Metodo de marcado con lucifrasa. - Google Patents
Metodo de marcado con lucifrasa.Info
- Publication number
- MX9701479A MX9701479A MX9701479A MX9701479A MX9701479A MX 9701479 A MX9701479 A MX 9701479A MX 9701479 A MX9701479 A MX 9701479A MX 9701479 A MX9701479 A MX 9701479A MX 9701479 A MX9701479 A MX 9701479A
- Authority
- MX
- Mexico
- Prior art keywords
- luciferase
- antibody
- adenosine triphosphate
- chemical entity
- luciferin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
Abstract
Se proporciona un método para conjugar luciferasa con una entidad química, especialmente con un agente de enlace específico como, por ejemplo, un anticuerpo, antígeno o bien ácido nucleico, y más especificamente un anticuerpo, que comprende (a) la mezcla de la luciferasa con uno varios de los siguientes: D-luciferina, iones magnesio y adenosintrifosfato y (b) realizar una reaccion de acoplamiento covalente entre la luciferasa y el reactivo de enlace usando un reactivo de acoplamiento donde la cantidad de D-luciferina, iones magnesio y/o adenosinatrifosfato es suficiente para proteger la actividad de la luciferasa contra la inhibicion por el reactivo de acoplamiento covalente. De preferencia el paso (a) se lleva a cabo mediante la mezcla de la luciferasa con sus sustratos en la solucion y de preferencia tanto el magnesio como el adenosintrifosfato se encuentran presentes como adenosintrifosfato de magnesio (Mg++ATP), opcionalmente junto con D-luciferina. En un segundo aspecto de la presente invencion, se proporciona una entidad química marcada que comprende una entidad química conjugada con luciferasa activa de conformidad con lo proporcionado por el método de la presente invencion. De preferencia, la entidad química es un agente de enlace especifico para su uso en un ensayo de enlace especifico, de preferencia un anticuerpo, antígeno o bien ácido nucleico. Cuando el agente de enlace es un ácido nucleico, es de preferencia un oligonucleotido, pero puede ser un polinucleotido o bien un nucleosido, y puede emplearse como sonda de hidratacion o bien como iniciador de extension de cadena, por ejemplo, un iniciador de reaccion en cadena de polimerasa. De manera más provechosa, la entidad es un anticuerpo puesto que intentos previos en inactividad. Se proporcionan adicionalmente conjuntos de implementos de prueba.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9417593A GB9417593D0 (en) | 1994-09-01 | 1994-09-01 | Luciferase labelling method |
| PCT/GB1995/002038 WO1996007100A2 (en) | 1994-09-01 | 1995-08-30 | Luciferase labelling method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX9701479A true MX9701479A (es) | 1998-02-28 |
Family
ID=10760673
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX9701479A MX9701479A (es) | 1994-09-01 | 1995-08-30 | Metodo de marcado con lucifrasa. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5837465A (es) |
| EP (1) | EP0778945B1 (es) |
| JP (1) | JP3594609B2 (es) |
| CN (1) | CN1100266C (es) |
| AU (1) | AU697586B2 (es) |
| BR (1) | BR9508652A (es) |
| DE (1) | DE69523060T2 (es) |
| GB (1) | GB9417593D0 (es) |
| IN (1) | IN183949B (es) |
| MX (1) | MX9701479A (es) |
| RU (1) | RU2199125C2 (es) |
| WO (1) | WO1996007100A2 (es) |
| ZA (1) | ZA957373B (es) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6171802B1 (en) * | 1998-06-10 | 2001-01-09 | Kent State University | Detection and amplification of ligands |
| WO2001046694A2 (en) * | 1999-12-22 | 2001-06-28 | Biosignal Packard Inc. | A bioluminescence resonance energy transfer (bret) fusion molecule and method of use |
| KR100414637B1 (ko) * | 2000-02-08 | 2004-01-13 | (주)에스제이바이오메드 | 사람 미토콘드리아 아데닐레이트 키나제 이소자임들에대한 항체와 면역학적 제제 및 심장질환 진단키트 |
| DE10137342A1 (de) * | 2001-07-31 | 2003-03-06 | Infineon Technologies Ag | Biosensor und Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren |
| JP2005509426A (ja) * | 2001-11-14 | 2005-04-14 | イギリス国 | 細胞内ａｔｐおよび／または遺伝子発現の測定方法 |
| FR2840687B1 (fr) * | 2002-06-05 | 2008-01-25 | Bio Merieux | Procede de detection simultanee de reactions d'hybridation et immunologiques et ses utilisations en diagnostic |
| DE60327775D1 (de) * | 2002-06-24 | 2009-07-09 | Exiqon As | Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen |
| US7996825B2 (en) * | 2003-10-31 | 2011-08-09 | Hewlett-Packard Development Company, L.P. | Cross-file inlining by using summaries and global worklist |
| ES2371664T3 (es) * | 2005-06-03 | 2012-01-09 | Beacon Biotechnology Llc | Ensayo de ácidos nucleicos de proximidad de quimioluminiscencia. |
| US20070254311A1 (en) * | 2006-04-26 | 2007-11-01 | Cardiogenics Inc. | Covalent modification and conjugation of luciferase |
| JP4604187B2 (ja) * | 2007-08-14 | 2010-12-22 | 独立行政法人産業技術総合研究所 | 糖鎖を介したルシフェラーゼと有機蛍光色素間のエネルギー移動を利用した可視−近赤外光プローブ |
| CN102159948B (zh) | 2008-07-22 | 2014-06-25 | 普罗美加公司 | 基于adp检测的发光磷酸转移酶或atp水解酶测定 |
| PL2635583T3 (pl) | 2010-11-02 | 2015-11-30 | Promega Corp | Pochodne koelenterazyny i metody ich wykorzystania |
| CA2817102C (en) | 2010-11-02 | 2020-07-28 | Promega Corporation | Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use |
| US10519483B2 (en) | 2012-02-21 | 2019-12-31 | Laboratory Corporation Of America Holdings | Methods and systems for rapid detection of microorganisms using infectious agents |
| CA2865308C (en) | 2012-02-21 | 2022-11-22 | Laboratory Corporation Of America Holdings | Methods and systems for detection of microorganisms |
| WO2014130760A1 (en) | 2013-02-22 | 2014-08-28 | Promega Corporation | Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates |
| JP6588917B2 (ja) | 2014-01-29 | 2019-10-09 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 生細胞への適用のためのプロ基質 |
| US9790537B2 (en) | 2014-01-29 | 2017-10-17 | Promega Corporation | Quinone-masked probes as labeling reagents for cell uptake measurements |
| EP3204509B1 (en) | 2014-10-08 | 2019-07-10 | Promega Corporation | Bioluminescent succinate detection assay |
| CN109957607A (zh) * | 2017-12-14 | 2019-07-02 | 深圳先进技术研究院 | 一种检测探针及其应用 |
| CA3140294A1 (en) | 2019-06-21 | 2020-12-24 | Jose S. GIL | Methods for producing mutant bacteriophages for the detection of listeria |
| RU2744869C2 (ru) * | 2019-08-08 | 2021-03-16 | Общество с ограниченной ответственностью "ПЛАНТА" | Метод и реактивы для детекции активности люциферазы |
| CN114729332A (zh) | 2019-08-26 | 2022-07-08 | 美国控股实验室公司 | 用于使用重组繁殖缺陷性指示噬菌体检测微生物的装置和方法 |
| JP2022547218A (ja) | 2019-09-11 | 2022-11-10 | ラボラトリー コーポレイション オブ アメリカ ホールディングス | 指標サブユニットを発現するように組換え感染病原体を使用して微生物を迅速に検出するための方法およびシステム |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5583024A (en) * | 1985-12-02 | 1996-12-10 | The Regents Of The University Of California | Recombinant expression of Coleoptera luciferase |
-
1994
- 1994-09-01 GB GB9417593A patent/GB9417593D0/en active Pending
-
1995
- 1995-08-30 BR BR9508652A patent/BR9508652A/pt not_active IP Right Cessation
- 1995-08-30 CN CN95195776A patent/CN1100266C/zh not_active Expired - Fee Related
- 1995-08-30 RU RU97105069/13A patent/RU2199125C2/ru not_active IP Right Cessation
- 1995-08-30 EP EP95929977A patent/EP0778945B1/en not_active Expired - Lifetime
- 1995-08-30 DE DE69523060T patent/DE69523060T2/de not_active Expired - Lifetime
- 1995-08-30 MX MX9701479A patent/MX9701479A/es unknown
- 1995-08-30 JP JP50856196A patent/JP3594609B2/ja not_active Expired - Lifetime
- 1995-08-30 AU AU33525/95A patent/AU697586B2/en not_active Ceased
- 1995-08-30 US US08/793,504 patent/US5837465A/en not_active Expired - Lifetime
- 1995-08-30 WO PCT/GB1995/002038 patent/WO1996007100A2/en active IP Right Grant
- 1995-08-31 IN IN1624DE1995 patent/IN183949B/en unknown
- 1995-09-01 ZA ZA957373A patent/ZA957373B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN1161746A (zh) | 1997-10-08 |
| BR9508652A (pt) | 1997-11-25 |
| DE69523060D1 (de) | 2001-11-08 |
| JP3594609B2 (ja) | 2004-12-02 |
| JPH10504903A (ja) | 1998-05-12 |
| ZA957373B (en) | 1996-05-20 |
| RU2199125C2 (ru) | 2003-02-20 |
| EP0778945A2 (en) | 1997-06-18 |
| DE69523060T2 (de) | 2002-06-20 |
| WO1996007100A3 (en) | 1996-05-02 |
| AU697586B2 (en) | 1998-10-08 |
| EP0778945B1 (en) | 2001-10-04 |
| IN183949B (es) | 2000-05-20 |
| CN1100266C (zh) | 2003-01-29 |
| US5837465A (en) | 1998-11-17 |
| WO1996007100A2 (en) | 1996-03-07 |
| GB9417593D0 (en) | 1994-10-19 |
| AU3352595A (en) | 1996-03-22 |
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