ES2165402T3 - Procedimiento para la deteccion sensible de acidos nucleicos. - Google Patents

Procedimiento para la deteccion sensible de acidos nucleicos.

Info

Publication number
ES2165402T3
ES2165402T3 ES95110768T ES95110768T ES2165402T3 ES 2165402 T3 ES2165402 T3 ES 2165402T3 ES 95110768 T ES95110768 T ES 95110768T ES 95110768 T ES95110768 T ES 95110768T ES 2165402 T3 ES2165402 T3 ES 2165402T3
Authority
ES
Spain
Prior art keywords
nucleic acid
sensitive detection
procedure
nucleic acids
hybridize
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95110768T
Other languages
English (en)
Inventor
Viola Rosemeyer
Rudolf Dr Seibl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4431269A external-priority patent/DE4431269A1/de
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of ES2165402T3 publication Critical patent/ES2165402T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

LA INVENCION SE REFIERE A UN PROCESO PARA LA COMPROBACION SENSIBLE DE UN ACIDO NUCLEICO OBJETIVO MEDIANTE HIBRIDIZACION CON UN ACIDO NUCLEICO DE SONDA, QUE CONTIENE UNA PARTE CON CAPACIDAD DE HIBRIDIZACION CON EL ACIDO NUCLEICO OBJETIVO Y UNA PARTE ESPECIFICA DE ACIDO NUCLEICO SIN CAPACIDAD DE HIBRIDIZACION CON EL ACIDO NUCLEICO OBJETIVO, CON DESDOBLAMIENTO DEL ACIDO NUCLEICO DE SONDA, HIBRIDIZACION DE UN PRODUCTO DE ESPACIO DEL ACIDO NUCLEICO DE SONDA, QUE NO CONTIENE PARTE HIBRIDIZADORA DE ACIDO NUCLEICO OBJETIVO, CON UN ACIDO NUCLEICO DE MATRIZ, QUE CONTIENE UNA PARTE HIBRIDIZABLE CON EL PRODUCTO DE ESPACIO Y UN PARTE NO HIBRIDIZABLE CON EL ACIDO NUCLEICO DE SONDA Y DETERMINACION DEL HIBRIDO A PARTIR DEL PRODUCTO DE ESPACIO Y EL ACIDO NUCLEICO DE MATRIZ ASI COMO UN REACTIVO APROPIADO PARA ELLO.
ES95110768T 1994-07-16 1995-07-11 Procedimiento para la deteccion sensible de acidos nucleicos. Expired - Lifetime ES2165402T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4425264 1994-07-16
DE4431269A DE4431269A1 (de) 1994-07-16 1994-09-02 Verfahren zum sensitiven Nachweis von Nukleinsäuren

Publications (1)

Publication Number Publication Date
ES2165402T3 true ES2165402T3 (es) 2002-03-16

Family

ID=25938437

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95110768T Expired - Lifetime ES2165402T3 (es) 1994-07-16 1995-07-11 Procedimiento para la deteccion sensible de acidos nucleicos.

Country Status (10)

Country Link
US (1) US5830664A (es)
EP (1) EP0705905B1 (es)
JP (1) JP3360977B2 (es)
KR (1) KR0161327B1 (es)
CN (1) CN1073160C (es)
AT (1) ATE206764T1 (es)
CA (1) CA2153944C (es)
ES (1) ES2165402T3 (es)
FI (1) FI114716B (es)
IL (1) IL114564A (es)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6153425A (en) * 1995-07-13 2000-11-28 Xtrana, Inc. Self-contained device integrating nucleic acid extraction, amplification and detection
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US20080160524A1 (en) * 1996-01-24 2008-07-03 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
EP2374890B1 (en) 1996-11-29 2012-11-14 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
WO1999022029A1 (en) * 1997-10-28 1999-05-06 The Regents Of The University Of California Dna base mismatch detection using flow cytometry
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
WO2002070755A2 (en) * 2000-11-15 2002-09-12 Third Wave Technologies, Inc. Fen endonucleases
WO2002064833A1 (fr) * 2001-02-15 2002-08-22 Takara Bio Inc. Procede de detection de polymorphisme de nucleotide
US6596489B2 (en) * 2001-03-30 2003-07-22 Applied Gene Technologies Methods and compositions for analyzing nucleotide sequence mismatches using RNase H
CA2466821A1 (en) * 2001-11-21 2003-06-05 Applera Corporation Digital assay
ATE485396T1 (de) * 2003-08-11 2010-11-15 Univ Georgia State Res Found Rna-nachweis und quantifizierung
KR100652903B1 (ko) * 2005-12-21 2006-12-04 한국과학기술연구원 초흡수성 고분자를 함유한 제습제의 제조 방법 및 그 제조장치
BRPI0908748A2 (pt) * 2008-03-15 2015-07-28 Hologic Inc Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação
US20090325169A1 (en) * 2008-04-30 2009-12-31 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
US10227641B2 (en) 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
CA2775143A1 (en) * 2009-09-25 2011-03-31 Alere San Diego, Inc. Detection of nucleic acids in crude matrices
US20110117559A1 (en) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Small rna detection assays
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
AU2013381709A1 (en) * 2013-03-15 2015-10-01 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US20200263233A1 (en) * 2017-06-23 2020-08-20 Eiken Kagaku Kabushiki Kaisha Method For Nucleic Acid Detection, Primer For Nucleic Acid Detection, And Kit For Nucleic Acid Detection
JP7333171B2 (ja) * 2018-12-18 2023-08-24 栄研化学株式会社 Ｒｎａ検出方法、ｒｎａ検出用核酸及びｒｎａ検出用キット
KR102261979B1 (ko) * 2019-10-28 2021-06-07 재단법인 대구경북첨단의료산업진흥재단 핵산분해효소 연쇄 반응

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
JP2856804B2 (ja) * 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5102784A (en) * 1990-05-04 1992-04-07 Oncor, Inc. Restriction amplification assay
AU2663292A (en) * 1991-09-10 1993-04-05 Jack D. Love Dna/rna target and signal amplification
ES2101116T3 (es) * 1991-09-13 1997-07-01 Cytocell Ltd Metodo de deteccion de una secuencia de acidos nucleicos.
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
US5518901A (en) * 1993-04-19 1996-05-21 Murtagh; James J. Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren

Also Published As

Publication number Publication date
JP3360977B2 (ja) 2003-01-07
ATE206764T1 (de) 2001-10-15
CA2153944C (en) 2002-12-24
KR0161327B1 (ko) 1998-11-16
IL114564A0 (en) 1995-11-27
FI953445A0 (fi) 1995-07-14
JPH0856700A (ja) 1996-03-05
FI953445A (fi) 1996-01-17
CN1073160C (zh) 2001-10-17
CA2153944A1 (en) 1996-01-17
EP0705905A3 (de) 1999-03-03
FI114716B (fi) 2004-12-15
CN1124294A (zh) 1996-06-12
US5830664A (en) 1998-11-03
EP0705905B1 (de) 2001-10-10
IL114564A (en) 1999-11-30
EP0705905A2 (de) 1996-04-10

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