ES2165402T3 - Procedimiento para la deteccion sensible de acidos nucleicos. - Google Patents
Procedimiento para la deteccion sensible de acidos nucleicos.Info
- Publication number
- ES2165402T3 ES2165402T3 ES95110768T ES95110768T ES2165402T3 ES 2165402 T3 ES2165402 T3 ES 2165402T3 ES 95110768 T ES95110768 T ES 95110768T ES 95110768 T ES95110768 T ES 95110768T ES 2165402 T3 ES2165402 T3 ES 2165402T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- sensitive detection
- procedure
- nucleic acids
- hybridize
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
LA INVENCION SE REFIERE A UN PROCESO PARA LA COMPROBACION SENSIBLE DE UN ACIDO NUCLEICO OBJETIVO MEDIANTE HIBRIDIZACION CON UN ACIDO NUCLEICO DE SONDA, QUE CONTIENE UNA PARTE CON CAPACIDAD DE HIBRIDIZACION CON EL ACIDO NUCLEICO OBJETIVO Y UNA PARTE ESPECIFICA DE ACIDO NUCLEICO SIN CAPACIDAD DE HIBRIDIZACION CON EL ACIDO NUCLEICO OBJETIVO, CON DESDOBLAMIENTO DEL ACIDO NUCLEICO DE SONDA, HIBRIDIZACION DE UN PRODUCTO DE ESPACIO DEL ACIDO NUCLEICO DE SONDA, QUE NO CONTIENE PARTE HIBRIDIZADORA DE ACIDO NUCLEICO OBJETIVO, CON UN ACIDO NUCLEICO DE MATRIZ, QUE CONTIENE UNA PARTE HIBRIDIZABLE CON EL PRODUCTO DE ESPACIO Y UN PARTE NO HIBRIDIZABLE CON EL ACIDO NUCLEICO DE SONDA Y DETERMINACION DEL HIBRIDO A PARTIR DEL PRODUCTO DE ESPACIO Y EL ACIDO NUCLEICO DE MATRIZ ASI COMO UN REACTIVO APROPIADO PARA ELLO.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4425264 | 1994-07-16 | ||
DE4431269A DE4431269A1 (de) | 1994-07-16 | 1994-09-02 | Verfahren zum sensitiven Nachweis von Nukleinsäuren |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2165402T3 true ES2165402T3 (es) | 2002-03-16 |
Family
ID=25938437
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES95110768T Expired - Lifetime ES2165402T3 (es) | 1994-07-16 | 1995-07-11 | Procedimiento para la deteccion sensible de acidos nucleicos. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5830664A (es) |
EP (1) | EP0705905B1 (es) |
JP (1) | JP3360977B2 (es) |
KR (1) | KR0161327B1 (es) |
CN (1) | CN1073160C (es) |
AT (1) | ATE206764T1 (es) |
CA (1) | CA2153944C (es) |
ES (1) | ES2165402T3 (es) |
FI (1) | FI114716B (es) |
IL (1) | IL114564A (es) |
Families Citing this family (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6872816B1 (en) * | 1996-01-24 | 2005-03-29 | Third Wave Technologies, Inc. | Nucleic acid detection kits |
US5994069A (en) | 1996-01-24 | 1999-11-30 | Third Wave Technologies, Inc. | Detection of nucleic acids by multiple sequential invasive cleavages |
US5846717A (en) * | 1996-01-24 | 1998-12-08 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
US6153425A (en) * | 1995-07-13 | 2000-11-28 | Xtrana, Inc. | Self-contained device integrating nucleic acid extraction, amplification and detection |
US7432048B2 (en) * | 1996-11-29 | 2008-10-07 | Third Wave Technologies, Inc. | Reactions on a solid surface |
US6706471B1 (en) * | 1996-01-24 | 2004-03-16 | Third Wave Technologies, Inc. | Detection of nucleic acid sequences by invader-directed cleavage |
US7122364B1 (en) | 1998-03-24 | 2006-10-17 | Third Wave Technologies, Inc. | FEN endonucleases |
US6875572B2 (en) | 1996-01-24 | 2005-04-05 | Third Wave Technologies, Inc. | Nucleic acid detection assays |
US7527928B2 (en) * | 1996-11-29 | 2009-05-05 | Third Wave Technologies, Inc. | Reactions on a solid surface |
US6913881B1 (en) | 1996-01-24 | 2005-07-05 | Third Wave Technologies, Inc. | Methods and compositions for detecting target sequences |
US5985557A (en) * | 1996-01-24 | 1999-11-16 | Third Wave Technologies, Inc. | Invasive cleavage of nucleic acids |
US20080160524A1 (en) * | 1996-01-24 | 2008-07-03 | Third Wave Technologies, Inc. | Methods and Compositions for Detecting Target Sequences |
US7195871B2 (en) * | 1996-01-24 | 2007-03-27 | Third Wave Technologies, Inc | Methods and compositions for detecting target sequences |
EP2374890B1 (en) | 1996-11-29 | 2012-11-14 | Third Wave Technologies, Inc. | Fen-1 endonucleases, mixtures and cleavage methods |
IL135852A0 (en) * | 1997-10-28 | 2001-05-20 | Univ California | A method for detecting dna base mismatch |
US8182991B1 (en) | 1997-11-26 | 2012-05-22 | Third Wave Technologies, Inc. | FEN-1 endonucleases, mixtures and cleavage methods |
US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
EP1548130B1 (en) * | 2000-11-15 | 2012-01-18 | Third Wave Technologies, Inc. | Fen endonucleases |
EP1367136A4 (en) * | 2001-02-15 | 2005-01-12 | Takara Bio Inc | METHOD FOR DETECTING NUCLEOTIDE POLYMORPHISM |
US6596489B2 (en) * | 2001-03-30 | 2003-07-22 | Applied Gene Technologies | Methods and compositions for analyzing nucleotide sequence mismatches using RNase H |
EP1448800A4 (en) * | 2001-11-21 | 2007-05-16 | Applera Corp | DIGITAL ASSAY |
US7354716B2 (en) * | 2003-08-11 | 2008-04-08 | Georgia State University Research Foundation, Inc. | RNA detection and quantitation |
KR100652903B1 (ko) * | 2005-12-21 | 2006-12-04 | 한국과학기술연구원 | 초흡수성 고분자를 함유한 제습제의 제조 방법 및 그 제조장치 |
CA2718011C (en) * | 2008-03-15 | 2015-05-19 | Hologic, Inc. | Compositions and methods for analysis of nucleic acid molecules during amplification reaction using footprint probes |
US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
EP2644710B1 (en) * | 2008-04-30 | 2016-12-21 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
CN105734169A (zh) * | 2009-09-25 | 2016-07-06 | 艾利尔圣迭戈公司 | 粗基质中的核酸的检测 |
WO2011060014A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
AU2013381709A1 (en) * | 2013-03-15 | 2015-10-01 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
WO2018235886A1 (ja) * | 2017-06-23 | 2018-12-27 | 栄研化学株式会社 | 核酸検出方法、核酸検出用プライマー及び核酸検出用キット |
JP7333171B2 (ja) * | 2018-12-18 | 2023-08-24 | 栄研化学株式会社 | Rna検出方法、rna検出用核酸及びrna検出用キット |
KR102261979B1 (ko) * | 2019-10-28 | 2021-06-07 | 재단법인 대구경북첨단의료산업진흥재단 | 핵산분해효소 연쇄 반응 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4994368A (en) * | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
DE68911648T2 (de) * | 1988-03-24 | 1994-06-23 | Univ Iowa Res Found | Katalytische hybridisierungs-systeme zum nachweis von nukleinsäuresequenzen, die auf deren aktivität als kofaktoren in katalytischen reaktionen basieren in denen eine komplementäre, markierte nukleinsäureprobe gespalten wird. |
US5232829A (en) * | 1989-09-29 | 1993-08-03 | Hoffmann-La Roche Inc. | Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes |
US5200314A (en) * | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
US5102784A (en) * | 1990-05-04 | 1992-04-07 | Oncor, Inc. | Restriction amplification assay |
AU2663292A (en) * | 1991-09-10 | 1993-04-05 | Jack D. Love | Dna/rna target and signal amplification |
DK0666927T3 (da) * | 1991-09-13 | 1997-09-15 | Cytocell Ltd | Fremgangsmåde til påvisning af en nukleinsyresekvens. |
US5422253A (en) * | 1992-12-07 | 1995-06-06 | Wisconsin Alumni Research Foundation | Method of site specific nucleic acid cleavage |
US5518901A (en) * | 1993-04-19 | 1996-05-21 | Murtagh; James J. | Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease |
DE4441626A1 (de) * | 1994-11-23 | 1996-05-30 | Boehringer Mannheim Gmbh | Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren |
-
1995
- 1995-07-11 EP EP95110768A patent/EP0705905B1/de not_active Expired - Lifetime
- 1995-07-11 AT AT95110768T patent/ATE206764T1/de active
- 1995-07-11 ES ES95110768T patent/ES2165402T3/es not_active Expired - Lifetime
- 1995-07-12 IL IL11456495A patent/IL114564A/xx not_active IP Right Cessation
- 1995-07-14 FI FI953445A patent/FI114716B/fi not_active IP Right Cessation
- 1995-07-14 JP JP17855095A patent/JP3360977B2/ja not_active Expired - Fee Related
- 1995-07-14 KR KR1019950020697A patent/KR0161327B1/ko not_active IP Right Cessation
- 1995-07-14 CN CN95115026A patent/CN1073160C/zh not_active Expired - Fee Related
- 1995-07-14 CA CA002153944A patent/CA2153944C/en not_active Expired - Fee Related
- 1995-07-17 US US08/503,058 patent/US5830664A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP3360977B2 (ja) | 2003-01-07 |
ATE206764T1 (de) | 2001-10-15 |
CN1124294A (zh) | 1996-06-12 |
KR0161327B1 (ko) | 1998-11-16 |
IL114564A0 (en) | 1995-11-27 |
US5830664A (en) | 1998-11-03 |
IL114564A (en) | 1999-11-30 |
EP0705905A2 (de) | 1996-04-10 |
CA2153944A1 (en) | 1996-01-17 |
CN1073160C (zh) | 2001-10-17 |
JPH0856700A (ja) | 1996-03-05 |
FI953445A0 (fi) | 1995-07-14 |
FI953445A (fi) | 1996-01-17 |
EP0705905B1 (de) | 2001-10-10 |
FI114716B (fi) | 2004-12-15 |
EP0705905A3 (de) | 1999-03-03 |
CA2153944C (en) | 2002-12-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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