EA200300284A1 - Способ амплификации нуклеиновых кислот - Google Patents

Способ амплификации нуклеиновых кислот

Info

Publication number
EA200300284A1
EA200300284A1 EA200300284A EA200300284A EA200300284A1 EA 200300284 A1 EA200300284 A1 EA 200300284A1 EA 200300284 A EA200300284 A EA 200300284A EA 200300284 A EA200300284 A EA 200300284A EA 200300284 A1 EA200300284 A1 EA 200300284A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleic acid
amplification
acid amplification
target nucleic
chimeric
Prior art date
Application number
EA200300284A
Other languages
English (en)
Other versions
EA005739B1 (ru
Inventor
Хироаки Сагава
Такаси Уемори
Хироюки Мукаи
Дзунко Ямамото
Дзун Томоно
Еидзи Кобаяси
Тацудзи Еноки
Киезо Асада
Икуносин Като
Original Assignee
Такара Био Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Такара Био Инк. filed Critical Такара Био Инк.
Publication of EA200300284A1 publication Critical patent/EA200300284A1/ru
Publication of EA005739B1 publication Critical patent/EA005739B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Abstract

Описываются способ высокочувствительной и специфической амплификации нуклеиновой кислоты-мишени в образце с использованием химерного олигонуклеотидного праймера, содержащего рибонуклеотид, расположенный на 3'-конце или в 3'-концевой области, эндорибонуклеазы и ДНК-полимеразы, обладающей активностью замещения цепи, т.е. способ изотермической инициируемой химерным праймером амплификации нуклеиновой кислоты (ICAN); способ детекции амплифицированного фрагмента, полученного с использованием вышеуказанного способа; способ получения нуклеиновой кислоты-мишени с использованием вышеуказанного способа амплификации и химерные олигонуклеотидные праймеры для использования в указанных способах.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200300284A 2000-08-23 2001-08-21 Способ амплификации нуклеиновых кислот EA005739B1 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2000251981 2000-08-23
JP2000284419 2000-09-19
JP2000288750 2000-09-22
JP2001104191 2001-04-03
PCT/JP2001/007139 WO2002016639A1 (fr) 2000-08-23 2001-08-21 Procede d'amplification d'acide nucleique

Publications (2)

Publication Number Publication Date
EA200300284A1 true EA200300284A1 (ru) 2003-08-28
EA005739B1 EA005739B1 (ru) 2005-06-30

Family

ID=27481553

Family Applications (2)

Application Number Title Priority Date Filing Date
EA200300284A EA005739B1 (ru) 2000-08-23 2001-08-21 Способ амплификации нуклеиновых кислот
EA200401354A EA007414B1 (ru) 2000-08-23 2001-08-21 Химерный олигонуклеотидный праймер для способа амплификации нуклеиновой кислоты; продукт для амплификации нуклеиновой кислоты; зонд для способа детекции нуклеиновой кислоты-мишени; материал с иммобилизованной амплифицированной нуклеиновой кислотой и способ его получения; способ определения нуклеотидной последовательности

Family Applications After (1)

Application Number Title Priority Date Filing Date
EA200401354A EA007414B1 (ru) 2000-08-23 2001-08-21 Химерный олигонуклеотидный праймер для способа амплификации нуклеиновой кислоты; продукт для амплификации нуклеиновой кислоты; зонд для способа детекции нуклеиновой кислоты-мишени; материал с иммобилизованной амплифицированной нуклеиновой кислотой и способ его получения; способ определения нуклеотидной последовательности

Country Status (9)

Country Link
EP (1) EP1312682A4 (ru)
JP (1) JP4128074B2 (ru)
KR (1) KR100735131B1 (ru)
CN (2) CN1254548C (ru)
AU (2) AU7878301A (ru)
CA (1) CA2417798A1 (ru)
EA (2) EA005739B1 (ru)
TW (1) TWI249577B (ru)
WO (1) WO2002016639A1 (ru)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US20040236094A1 (en) * 2000-09-19 2004-11-25 Hiroaki Sagawa Method of forming complex
DE60142709D1 (de) 2000-12-13 2010-09-09 Nugen Technologies Inc Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
WO2002062993A1 (fr) * 2001-02-06 2002-08-15 Takara Bio Inc. Acides nucleiques amplifies et produits immobilises de ces derniers
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US7838270B2 (en) 2001-05-22 2010-11-23 The University Of Chicago Target-dependent transcription using deletion mutants of N4 RNA polymerase
EA006679B1 (ru) * 2001-06-12 2006-02-24 Такара Био Инк. Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения
US7056671B2 (en) 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
AU2003211421A1 (en) * 2002-03-07 2003-09-16 Takara Bio Inc. Method of detecting base substitution
EP1553172B1 (en) 2002-08-30 2009-06-03 Takara Bio Inc. Thermostable ribonuclease h
EP1564301A4 (en) * 2002-10-29 2007-07-11 Riken METHOD FOR AMPLIFYING NUCLEIC ACID
AU2003294447A1 (en) 2002-11-21 2004-06-18 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US7402386B2 (en) 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
KR100841128B1 (ko) * 2003-05-16 2008-06-24 하우스 쇼쿠힝 가부시키가이샤 식품 또는 식품 원재료 중의 특정 식물속의 정량적 ｐｃｒ검출법
ATE496141T1 (de) * 2003-12-06 2011-02-15 Abbott Lab Verfahren und system zum analysieren von reaktionen unter verwendung eines informationssystems
JP4249186B2 (ja) * 2003-12-10 2009-04-02 タカラバイオ株式会社 核酸の増幅方法
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
DE102005019112A1 (de) 2005-04-25 2006-10-26 Siemens Ag Kombinationsantrieb mit Hybridreluktanzmotor
EP1883707B1 (en) 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
CA2622649C (en) 2007-03-09 2018-04-24 Riken Nucleic acid amplification method using primer exhibiting exciton effect
WO2009042291A2 (en) 2007-08-03 2009-04-02 Igor Kutyavin Accelerated cascade amplification (aca) of nucleic acids comprising strand and sequence specific dna nicking
US11041215B2 (en) * 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US9090948B2 (en) 2008-09-30 2015-07-28 Abbott Molecular Inc. Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples
JP5652843B2 (ja) * 2008-10-17 2015-01-14 独立行政法人農業・食品産業技術総合研究機構 Ｄｎａ増幅法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
DK2376517T3 (da) 2008-10-24 2013-02-11 Epict Technologies Corp Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP5616584B2 (ja) * 2009-01-09 2014-10-29 富士フイルム株式会社 核酸配列の複製方法
US20120100545A1 (en) * 2009-07-03 2012-04-26 Tan Tock Seng Hospital Method and/or primers for the detection of mycobacterium tuberculosis
WO2011037802A2 (en) 2009-09-28 2011-03-31 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
US8618253B2 (en) * 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
CN101921846B (zh) * 2010-07-20 2012-11-14 重庆大学 一种多重基因表达的分析方法
WO2012013734A1 (en) * 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Control nucleic acids for multiple parameters
JP5711234B2 (ja) 2010-07-29 2015-04-30 タカラバイオ株式会社 標的塩基検出用ｒｎａ含有プローブの製造方法
EP2418286A1 (en) * 2010-08-10 2012-02-15 QIAGEN GmbH Improved method for isothermal amplification of nucleic acids
JP6126381B2 (ja) 2010-12-10 2017-05-10 国立大学法人東京工業大学 標的核酸の検出方法及びキット
CN102181547A (zh) * 2011-04-13 2011-09-14 北京三元食品股份有限公司 金黄色葡萄球菌肠毒素a、b基因pcr同步检测试剂盒
GB201112140D0 (en) * 2011-07-14 2011-08-31 Dna Electronics Nucleic acid amplification
JP5299981B1 (ja) 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
CA2936157C (en) 2014-01-15 2021-08-24 Tokyo Institute Of Technology Covered sequence conversion dna and detection methods
EA037029B1 (ru) 2014-03-31 2021-01-28 КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ Флуоресцентно меченная одноцепочечная нуклеиновая кислота и ее применение
AU2015277059B2 (en) 2014-06-18 2021-06-17 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
WO2016011280A1 (en) 2014-07-16 2016-01-21 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
EP3183356A4 (en) * 2014-08-18 2018-04-11 Molecular Assemblies Inc. Methods and apparatus for synthesizing nucleic acids
EP3207159B1 (en) * 2014-10-14 2019-11-20 Abbott Laboratories Sequence conversion and signal amplifier dna having poly dna spacer sequences and detection methods using same
ES2706531T3 (es) 2014-11-11 2019-03-29 Illumina Inc Amplificación de polinucleótidos empleando sistemas CRISPR-Cas
US10604790B2 (en) 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
CN104531696A (zh) * 2014-12-31 2015-04-22 深圳华大基因研究院 引物组合物及其用途
EP3337909B1 (en) * 2015-09-24 2020-03-18 Sigma Aldrich Co. LLC Methods and reagents for molecular proximity detection using rna-guided nucleic acid binding proteins
US11046939B2 (en) 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
WO2017106790A1 (en) 2015-12-18 2017-06-22 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
KR101804655B1 (ko) 2016-01-21 2018-01-10 연세대학교 산학협력단 생물학적 서열의 유사매듭 구조 판단 장치 및 방법
EP3455372B1 (en) 2016-05-11 2019-10-30 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
GB201612011D0 (en) * 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
CN113151423A (zh) * 2016-08-05 2021-07-23 生物辐射实验室股份有限公司 第二链引导
WO2018129368A2 (en) 2017-01-06 2018-07-12 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
RU2699522C2 (ru) * 2017-11-30 2019-09-05 Общество с ограниченной ответственностью "ИБМХ-ЭкоБиоФарм" (ООО "ИБМХ-ЭкоБиоФарм") Способ ферментативного получения модифицированных ДНК для создания реагентов, специфично связывающихся с гидрофобными участками высокомолекулярных органических соединений
CN111989408A (zh) 2018-04-20 2020-11-24 株式会社艾匹基乃龙 检测靶核酸区域内的与参考序列的差异的方法
CN108588050B (zh) * 2018-05-14 2021-06-25 北京艾克伦医疗科技有限公司 Dna聚合酶以及核酸检测方法和试剂盒
CN108642144B (zh) * 2018-05-18 2020-06-09 贠红岩 一种恒温链置换扩增技术及试剂盒
CN108841919B (zh) * 2018-06-12 2021-07-06 艾吉泰康生物科技(北京)有限公司 一种嵌合式sda法制备探针
CN109022550A (zh) * 2018-07-09 2018-12-18 中国疾病预防控制中心传染病预防控制所 检测a型肉毒梭菌的实时荧光定量pcr核酸序列以及试剂盒
CN108913736B (zh) * 2018-07-10 2021-08-24 中国海洋大学 单链寡核苷酸的制备方法
CN109402268A (zh) * 2018-10-29 2019-03-01 湖南健基生物技术有限公司 一种检测人cyp3a4基因多态性的组合物、试剂盒、样品处理方法和应用
SG11202107921SA (en) * 2019-02-12 2021-08-30 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CN113528624A (zh) * 2020-04-17 2021-10-22 青岛大学 扩增和检测核酸的方法及试剂盒
AU2022234741A1 (en) 2021-03-09 2023-09-14 Illumina Cambridge Limited Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins
AU2022232600A1 (en) 2021-03-09 2023-09-14 Illumina, Inc. Analyzing expression of protein-coding variants in cells
AU2022328378A1 (en) 2021-08-11 2024-01-18 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
JPH11513881A (ja) * 1995-06-07 1999-11-30 ジェン−プロープ・インコーポレーテッド テンプレートおよびプライマーに基づく酵素的に切断可能なオリゴヌクレオチドの合成
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
WO2000056877A1 (fr) * 1999-03-19 2000-09-28 Takara Shuzo Co., Ltd. Procede d'amplification d'une sequence d'acide nucleique
WO2002062993A1 (fr) * 2001-02-06 2002-08-15 Takara Bio Inc. Acides nucleiques amplifies et produits immobilises de ces derniers

Also Published As

Publication number Publication date
TWI249577B (en) 2006-02-21
KR20030036707A (ko) 2003-05-09
EP1312682A4 (en) 2005-03-09
CN1854309A (zh) 2006-11-01
EA200401354A1 (ru) 2005-02-24
KR100735131B1 (ko) 2007-07-03
CN1254548C (zh) 2006-05-03
AU2001278783B2 (en) 2006-04-27
CA2417798A1 (en) 2003-01-30
EP1312682A1 (en) 2003-05-21
EA007414B1 (ru) 2006-10-27
JP4128074B2 (ja) 2008-07-30
WO2002016639A1 (fr) 2002-02-28
EA005739B1 (ru) 2005-06-30
AU7878301A (en) 2002-03-04
CN1471588A (zh) 2004-01-28

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