EA200400021A1 - Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения - Google Patents

Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения

Info

Publication number
EA200400021A1
EA200400021A1 EA200400021A EA200400021A EA200400021A1 EA 200400021 A1 EA200400021 A1 EA 200400021A1 EA 200400021 A EA200400021 A EA 200400021A EA 200400021 A EA200400021 A EA 200400021A EA 200400021 A1 EA200400021 A1 EA 200400021A1
Authority
EA
Eurasian Patent Office
Prior art keywords
reagent
nucleic acid
detection
amplification
stabilization
Prior art date
Application number
EA200400021A
Other languages
English (en)
Other versions
EA006679B1 (ru
Inventor
Хироаки Сагава
Такаси Уемори
Хироюки Мукаи
Дзунко Ямамото
Дзун Томоно
Ейдзи Кобаяси
Тацудзи Еноки
Киезо Асада
Икуносин Като
Original Assignee
Такара Био Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Такара Био Инк. filed Critical Такара Био Инк.
Publication of EA200400021A1 publication Critical patent/EA200400021A1/ru
Publication of EA006679B1 publication Critical patent/EA006679B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Описываются способ стабилизации реагента для реакции для высокочувствительной и специфической амплификации нуклеиновой кислоты-мишени в образце с использованием химерного олигонуклеотидного праймера и способ его хранения в течение длительного времени; и способ высокочувствительной детекции патогенного микроорганизма и вируса.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200400021A 2001-06-12 2002-06-12 Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения EA006679B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2001177737 2001-06-12
JP2001249689 2001-08-20
PCT/JP2002/005832 WO2002101042A1 (fr) 2001-06-12 2002-06-12 Procede de stabilisation d'un reactif pour amplifier ou detecter l'acide nucleique et procede de memorisation

Publications (2)

Publication Number Publication Date
EA200400021A1 true EA200400021A1 (ru) 2004-04-29
EA006679B1 EA006679B1 (ru) 2006-02-24

Family

ID=26616789

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200400021A EA006679B1 (ru) 2001-06-12 2002-06-12 Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения

Country Status (9)

Country Link
US (1) US20050059000A1 (ru)
EP (1) EP1396538A4 (ru)
JP (1) JPWO2002101042A1 (ru)
KR (1) KR20040007620A (ru)
CN (1) CN100379861C (ru)
AU (1) AU2002311183B2 (ru)
CA (1) CA2450397A1 (ru)
EA (1) EA006679B1 (ru)
WO (1) WO2002101042A1 (ru)

Families Citing this family (18)

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US20120045747A1 (en) * 2010-08-23 2012-02-23 Samsung Techwin Co., Ltd. Kit for detecting hepatitis b virus and method for detecting hepatitis b virus using the same
KR20100060018A (ko) * 2005-03-09 2010-06-04 재단법인 목암생명공학연구소 작은 간섭 ｒｎａ 및 이를 포함하는 ｂ형 간염 바이러스 치료용 약학 조성물
KR20080066993A (ko) * 2005-11-07 2008-07-17 지멘스 헬쓰케어 다이아그노스틱스 인크. 클라미디아 트라코마티스 특이적 올리고뉴클레오티드 서열
DE102006028101B4 (de) * 2006-06-19 2014-02-13 Siemens Aktiengesellschaft Verfahren zur Analyse von amplifizierten Nukleinsäuren
CA2653988C (en) 2007-04-13 2017-05-30 Abbott Laboratories Primer and probe sequences for detecting chlamydia trachomatis
US20140038174A1 (en) * 2011-04-26 2014-02-06 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples
US8900807B2 (en) 2008-02-25 2014-12-02 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Composition and methods for rapid detection of HIV by loop-mediated isothermal amplification (LAMP)
EP2333109B1 (en) * 2008-09-03 2013-08-07 Takara Bio, Inc. Composition for detection of rna
JP5608997B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
CN102656277A (zh) * 2009-07-03 2012-09-05 新加坡科技研究局 用于检测结核分枝杆菌的方法和/或引物
WO2016039377A1 (ja) 2014-09-11 2016-03-17 タカラバイオ株式会社 耐熱性のミスマッチエンドヌクレアーゼの利用方法
US9617587B1 (en) * 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
CN110527713B (zh) * 2019-07-02 2024-01-30 黄天谊 一种折叠引物的pcr扩增方法
CN111424100A (zh) * 2020-02-25 2020-07-17 宁波明舟生物科技有限公司 一种肺炎军团菌、脑膜炎奈瑟菌、肺炎支原体的检测方法
CN112501324A (zh) * 2020-11-26 2021-03-16 广州迪澳生物科技有限公司 一种基于环介导等温扩增检测结核分枝杆菌复合群及非结核分枝杆菌复合群的引物、试剂盒
CN112941208B (zh) * 2021-01-28 2023-07-14 四川大学 一种用于食品中沙门氏菌的现场快速检测方法
CN112941148A (zh) * 2021-03-03 2021-06-11 通用生物系统（安徽）有限公司 一种核酸引物保存液的制备方法

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
ES2141088T3 (es) * 1990-02-16 2000-03-16 Hoffmann La Roche Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
DK0590327T3 (da) * 1992-09-11 2003-07-28 Hoffmann La Roche Påvisning af nukleinsyrer i blod
WO1995025180A1 (en) * 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
GB9717061D0 (en) * 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
ATE374833T1 (de) * 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
NO308925B1 (no) * 1999-03-15 2000-11-13 Dimension Technologies As FremgangsmÕte og apparat for stereoprojeksjon av bilder
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NZ517121A (en) * 1999-09-13 2004-05-28 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
AU7878301A (en) * 2000-08-23 2002-03-04 Takara Shuzo Co Method of amplifying nucleic acid

Also Published As

Publication number Publication date
WO2002101042A1 (fr) 2002-12-19
CN100379861C (zh) 2008-04-09
JPWO2002101042A1 (ja) 2005-04-07
CN1541267A (zh) 2004-10-27
EP1396538A4 (en) 2005-10-26
EP1396538A1 (en) 2004-03-10
AU2002311183B2 (en) 2007-03-01
US20050059000A1 (en) 2005-03-17
KR20040007620A (ko) 2004-01-24
EA006679B1 (ru) 2006-02-24
CA2450397A1 (en) 2002-12-19

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU