ATE428002T1 - Nachweis von immobilisierter nukleinsaure - Google Patents
Nachweis von immobilisierter nukleinsaureInfo
- Publication number
- ATE428002T1 ATE428002T1 AT04789513T AT04789513T ATE428002T1 AT E428002 T1 ATE428002 T1 AT E428002T1 AT 04789513 T AT04789513 T AT 04789513T AT 04789513 T AT04789513 T AT 04789513T AT E428002 T1 ATE428002 T1 AT E428002T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- immobilized nucleic
- methods
- dye compound
- solid
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 7
- 102000039446 nucleic acids Human genes 0.000 title abstract 7
- 150000007523 nucleic acids Chemical class 0.000 title abstract 7
- 238000001514 detection method Methods 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 6
- 238000000034 method Methods 0.000 abstract 6
- 239000000975 dye Substances 0.000 abstract 5
- 239000007787 solid Substances 0.000 abstract 4
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 abstract 2
- 229960005542 ethidium bromide Drugs 0.000 abstract 2
- 239000011521 glass Substances 0.000 abstract 2
- 231100000804 nongenotoxic Toxicity 0.000 abstract 2
- 239000012192 staining solution Substances 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 229920000936 Agarose Polymers 0.000 abstract 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 abstract 1
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 1
- 239000007983 Tris buffer Substances 0.000 abstract 1
- 239000011324 bead Substances 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 239000011859 microparticle Substances 0.000 abstract 1
- 239000003471 mutagenic agent Substances 0.000 abstract 1
- 231100000707 mutagenic chemical Toxicity 0.000 abstract 1
- 230000003505 mutagenic effect Effects 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- ACOJCCLIDPZYJC-UHFFFAOYSA-M thiazole orange Chemical class CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2C(C=C3N(C4=CC=CC=C4S3)C)=CC=[N+](C)C2=C1 ACOJCCLIDPZYJC-UHFFFAOYSA-M 0.000 abstract 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
- C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
- C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
- C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Plural Heterocyclic Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50763003P | 2003-09-30 | 2003-09-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE428002T1 true ATE428002T1 (de) | 2009-04-15 |
Family
ID=34421643
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04789513T ATE428002T1 (de) | 2003-09-30 | 2004-09-30 | Nachweis von immobilisierter nukleinsaure |
Country Status (7)
| Country | Link |
|---|---|
| US (7) | US7727716B2 (de) |
| EP (2) | EP1668162B1 (de) |
| JP (2) | JP4651621B2 (de) |
| AT (1) | ATE428002T1 (de) |
| CA (1) | CA2540508A1 (de) |
| DE (1) | DE602004020484D1 (de) |
| WO (1) | WO2005033342A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005033342A1 (en) * | 2003-09-30 | 2005-04-14 | Molecular Probes, Inc. | Detection of immobilized nucleic acid |
| US7601498B2 (en) * | 2005-03-17 | 2009-10-13 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection and associated technology |
| US7776567B2 (en) * | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| EP2062036A4 (de) * | 2006-08-31 | 2016-09-21 | Life Technologies Israel Ltd | Verfahren, kassetten, gele und vorrichtungen zur isolation und sammlung von biomolekülen aus elektrophorese-gelen |
| ATE476478T1 (de) * | 2006-11-02 | 2010-08-15 | Hoffmann La Roche | Neue dsdna-bindende fluoreszenzfarbstoffe |
| EP2201009B1 (de) | 2007-10-02 | 2016-10-26 | Thermo Fisher Scientific Inc. | Monomethin-farbstoffe |
| US8877437B1 (en) | 2009-12-23 | 2014-11-04 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection |
| EP2697730A4 (de) | 2011-04-15 | 2015-04-15 | Advanced Diamond Technologies Inc | Elektrochemisches system und verfahren zur vor-ort-erzeugung von oxidationsmitteln bei hoher stromdichte |
| JP2013040136A (ja) * | 2011-08-17 | 2013-02-28 | Tosoh Corp | ４−アルコキシ−２−（４−アミノスチリル）ベンゾチアゾリウム塩、その製造方法およびそれを用いる核酸の検出方法 |
| EP2928965B1 (de) * | 2012-12-07 | 2018-07-04 | Life Technologies Corporation | Nachweis von rna-molekülen mittels substituierter unsymmetrischer cyaninfarbstoffe |
| US10011570B2 (en) | 2013-10-03 | 2018-07-03 | Biotium, Inc. | Nucleic acid binding dyes with improved safety |
| CN104833668B (zh) * | 2014-06-11 | 2017-05-24 | 南方医科大学 | 苯并吲哚半菁染料检测双氧水的用途 |
| EP3235909A1 (de) * | 2016-04-20 | 2017-10-25 | SFC Co., Ltd. | Auf merocyanin basierende verbindungen und farbstoffe, kits und kontrastmittelzusammensetzungen zur markierung von biomolekülen damit |
| ES2954485T3 (es) * | 2017-09-30 | 2023-11-22 | Bioatlas Oue | Comprimido multicomponente para la preparación de gel de agarosa y métodos del mismo |
| WO2023235392A1 (en) * | 2022-06-02 | 2023-12-07 | Perkinelmer Health Sciences, Inc. | Electrophoresis-mediated characterization of dna content of adeno-associated virus capsids |
| WO2024081869A1 (en) | 2022-10-14 | 2024-04-18 | 10X Genomics, Inc. | Methods for analysis of biological samples |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883867A (en) | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| US4957870A (en) | 1985-11-01 | 1990-09-18 | Becton, Dickinson And Company | Detection of Reticulocytes, RNA and DNA |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| US5436134A (en) | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5534416A (en) | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| US5656449A (en) * | 1995-03-06 | 1997-08-12 | Molecular Probes, Inc. | Neutral unsymmetrical cyanine dyes |
| US7824532B2 (en) | 1995-04-26 | 2010-11-02 | Life Technologies Corporation | Apparatus and method for electrophoresis |
| US5582702A (en) * | 1995-04-26 | 1996-12-10 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US6379516B1 (en) * | 1995-04-26 | 2002-04-30 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| US5929227A (en) * | 1995-07-12 | 1999-07-27 | The Regents Of The University Of California | Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores |
| CA2283267A1 (en) | 1997-03-07 | 1998-09-11 | Clare Chemical Research Llc | Fluorometric detection using visible light |
| US5865874A (en) * | 1997-06-27 | 1999-02-02 | Duracell Inc. | Hydrogen storage alloy |
| EP1057001A2 (de) | 1998-02-19 | 2000-12-06 | Edvotek | Hergestellte vorrichtungen und methoden zum färben von biomolekülen |
| US6562213B1 (en) * | 2000-08-30 | 2003-05-13 | Ethrog Biotechnology Ltd. | Electrophoresis apparatus for simultaneous loading of multiple samples |
| CA2440129A1 (en) | 2001-03-08 | 2002-09-12 | Ethrog Biotechnology Ltd. | Apparatus and method for electrophoresis |
| WO2005033342A1 (en) * | 2003-09-30 | 2005-04-14 | Molecular Probes, Inc. | Detection of immobilized nucleic acid |
-
2004
- 2004-09-30 WO PCT/US2004/032693 patent/WO2005033342A1/en active Application Filing
- 2004-09-30 JP JP2006534241A patent/JP4651621B2/ja active Active
- 2004-09-30 DE DE602004020484T patent/DE602004020484D1/de active Active
- 2004-09-30 US US10/956,868 patent/US7727716B2/en active Active
- 2004-09-30 EP EP04789513A patent/EP1668162B1/de active Active
- 2004-09-30 CA CA002540508A patent/CA2540508A1/en not_active Abandoned
- 2004-09-30 EP EP08158255A patent/EP1988177A1/de not_active Withdrawn
- 2004-09-30 AT AT04789513T patent/ATE428002T1/de not_active IP Right Cessation
-
2010
- 2010-04-30 US US12/771,897 patent/US7977057B2/en active Active
- 2010-05-12 US US12/778,774 patent/US20100227331A1/en not_active Abandoned
- 2010-11-16 JP JP2010255930A patent/JP2011099862A/ja active Pending
-
2011
- 2011-08-30 US US13/221,208 patent/US20120058566A1/en not_active Abandoned
-
2012
- 2012-06-18 US US13/525,690 patent/US20130011927A1/en not_active Abandoned
-
2013
- 2013-05-15 US US13/895,207 patent/US8969004B2/en active Active
-
2015
- 2015-03-02 US US14/636,063 patent/US20150247799A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1988177A1 (de) | 2008-11-05 |
| US7727716B2 (en) | 2010-06-01 |
| JP2007507238A (ja) | 2007-03-29 |
| US20150247799A1 (en) | 2015-09-03 |
| EP1668162B1 (de) | 2009-04-08 |
| US20050239096A1 (en) | 2005-10-27 |
| JP4651621B2 (ja) | 2011-03-16 |
| US7977057B2 (en) | 2011-07-12 |
| US20120058566A1 (en) | 2012-03-08 |
| EP1668162A1 (de) | 2006-06-14 |
| JP2011099862A (ja) | 2011-05-19 |
| CA2540508A1 (en) | 2005-04-14 |
| US20100227331A1 (en) | 2010-09-09 |
| US20100216665A1 (en) | 2010-08-26 |
| US20130011927A1 (en) | 2013-01-10 |
| US8969004B2 (en) | 2015-03-03 |
| DE602004020484D1 (de) | 2009-05-20 |
| WO2005033342A1 (en) | 2005-04-14 |
| US20140076726A1 (en) | 2014-03-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |