ATE280243T1 - Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits - Google Patents

Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits

Info

Publication number
ATE280243T1
ATE280243T1 AT95302921T AT95302921T ATE280243T1 AT E280243 T1 ATE280243 T1 AT E280243T1 AT 95302921 T AT95302921 T AT 95302921T AT 95302921 T AT95302921 T AT 95302921T AT E280243 T1 ATE280243 T1 AT E280243T1
Authority
AT
Austria
Prior art keywords
nucleic acids
fluorescent dyes
double strand
strand nucleic
detecting double
Prior art date
Application number
AT95302921T
Other languages
English (en)
Inventor
John W Sutherland
David R Patterson
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Application granted granted Critical
Publication of ATE280243T1 publication Critical patent/ATE280243T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Luminescent Compositions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT95302921T 1994-04-29 1995-04-28 Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits ATE280243T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23539694A 1994-04-29 1994-04-29

Publications (1)

Publication Number Publication Date
ATE280243T1 true ATE280243T1 (de) 2004-11-15

Family

ID=22885321

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95302921T ATE280243T1 (de) 1994-04-29 1995-04-28 Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits

Country Status (8)

Country Link
US (1) US5563037A (de)
EP (1) EP0684316B1 (de)
JP (1) JP4260903B2 (de)
KR (1) KR100388732B1 (de)
CN (1) CN1100884C (de)
AT (1) ATE280243T1 (de)
CA (1) CA2148140C (de)
DE (1) DE69533660T2 (de)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US7273749B1 (en) 1990-06-04 2007-09-25 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US6180403B1 (en) * 1999-10-28 2001-01-30 Isis Pharmaceuticals Inc. Antisense inhibition of tumor necrosis factor alpha converting enzyme (TACE) expression
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
JP2001501454A (ja) * 1996-05-17 2001-02-06 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー プライマーに制御された増幅産物の蛍光検出による標的細菌の同定
SE506700C2 (sv) * 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
AU726501B2 (en) 1996-06-04 2000-11-09 University Of Utah Research Foundation Monitoring hybridization during PCR
US6312930B1 (en) 1996-09-16 2001-11-06 E. I. Du Pont De Nemours And Company Method for detecting bacteria using PCR
WO1998036096A1 (en) * 1997-02-14 1998-08-20 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
EP2316571A3 (de) 1998-05-01 2011-07-27 Gen-Probe Incorporated Automatisches Diagnoseanalysegerät und Verfahren
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
DE60042370D1 (de) 1999-12-21 2009-07-23 Ortho Clinical Diagnostics Inc Detektion von nukleinsäuren
US7354707B2 (en) 2000-06-27 2008-04-08 National Institute Of Advanced Industrial Science And Technology Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
WO2002014548A1 (en) * 2000-08-10 2002-02-21 Applied Gene Technologies, Inc. Compositions and methods for nucleic acids sample processing and amplification
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
ES2301754T3 (es) * 2002-02-12 2008-07-01 University Of Utah Research Foundation Analisis de pruebas multiples de amplificaciones de acidos nucleicos en tiempo real.
AU2003288906C1 (en) * 2002-09-20 2010-12-09 Yale University Riboswitches, methods for their use, and compositions for use with riboswitches.
ES2438967T3 (es) * 2002-10-23 2014-01-21 University Of Utah Research Foundation Análisis de fusión de amplicones con colorantes de saturación
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
AU2003301825A1 (en) * 2002-10-31 2004-06-07 Pfizer Products, Inc. Panels of molecular targets differentially expressed during cd8+ t-cell priming
US20040191803A1 (en) * 2002-11-22 2004-09-30 Michela Gallagher Target for therapy of cognitive impairment
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
EP2261371B1 (de) 2002-12-20 2015-07-22 QIAGEN GmbH Nukleinsäureamplifikation
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
ATE355529T1 (de) * 2003-08-20 2006-03-15 Sysmex Corp Probenanalysegerät und eine vorrichtung zur detektion von nukleinsäuren
KR100906749B1 (ko) * 2004-03-25 2009-07-09 (주)바이오니아 인터컬레이팅 형광염료가 표지된 프로브를 이용한 핵산 증폭 측정 방법
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
US20060275798A1 (en) * 2005-04-04 2006-12-07 Steichen John C Detection of organisms using a media sachet and primer directed nucleic acid amplification
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
US20060246513A1 (en) * 2005-05-02 2006-11-02 Bohannon Robert C Method and device to detect the presence of analytes in a sample
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7781165B2 (en) * 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
US8313901B2 (en) * 2005-12-21 2012-11-20 Yale University Methods and compositions related to the modulation of riboswitches
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
JP2010502752A (ja) * 2006-09-11 2010-01-28 イェール ユニバーシティー リシンリボスイッチ、リシンリボスイッチを用いた構造に基づく化合物設計、ならびにリシンリボスイッチを用いた使用のための方法および組成物
CN101801185A (zh) 2007-03-22 2010-08-11 耶鲁大学 与控制可变剪接的核糖开关有关的方法和组合物
JP2010528616A (ja) * 2007-05-29 2010-08-26 イェール ユニバーシティー オルタナティブスプライシングとrnaのスプライシングを調節するリボスイッチに関連する方法と組成物
WO2008156987A2 (en) 2007-05-29 2008-12-24 Yale University Riboswitches and methods and compositions for use of and with riboswitches
US20100285473A1 (en) 2009-01-27 2010-11-11 John Wolff Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
EP2430161A1 (de) 2009-05-15 2012-03-21 Yale University Gemm-riboswitches, strukturbasierte verbindungskonstruktion mit gemm-riboswitches sowie verfahren und zusammensetzungen zur verwendung von und mit gemm-riboswitches
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US20120321647A1 (en) 2010-01-12 2012-12-20 Yale University Structured rna motifs and compounds and methods for their use
US8628914B2 (en) 2010-05-26 2014-01-14 Qiagen Gaithersburg, Inc. Quantitative helicase assay
EP2754496A3 (de) 2010-07-23 2017-08-02 Beckman Coulter, Inc. System oder Verfahren zur Aufnahme analytischer Einheiten
WO2012021554A1 (en) 2010-08-09 2012-02-16 Yale University Cyclic di-gmp-ii riboswitches, motifs, and compounds, and methods for their use
CN102095705A (zh) * 2010-12-09 2011-06-15 山东理工大学 一种基于激光扫描共聚焦显微镜的头发丝成像方法
US9580713B2 (en) 2011-09-17 2017-02-28 Yale University Fluoride-responsive riboswitches, fluoride transporters, and methods of use
BR112014011048A2 (pt) 2011-11-07 2017-05-02 Beckman Coulter Inc braço robótico
KR20140091033A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 컨테이너 검출
ES2729283T3 (es) 2011-11-07 2019-10-31 Beckman Coulter Inc Sistema de centrífuga y flujo de trabajo
EP3373015A1 (de) 2011-11-07 2018-09-12 Beckman Coulter Inc. Aliquotierungssystem und arbeitsablauf
BR112014010955A2 (pt) 2011-11-07 2017-06-06 Beckman Coulter Inc sistema e método para processar amostras
EP2776847A1 (de) 2011-11-07 2014-09-17 Beckman Coulter, Inc. Magnetische dämpfung für probentransportsystem
WO2014152775A2 (en) 2013-03-14 2014-09-25 Certirx Corporation Nucleic acid-based authentication and identification codes
CN110426442B (zh) * 2016-06-13 2022-02-11 苏州优逸兰迪生物科技有限公司 一种核酸凝胶电泳测试的方法
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4544546A (en) * 1980-04-21 1985-10-01 Abbott Laboratories Fluorescent nucleic acid stains
CA1155041A (en) * 1980-04-21 1983-10-11 Michael E. Jolley Fluorescent nucleic acid stains
US4777129A (en) * 1983-12-12 1988-10-11 Molecular Diagnostics, Inc. Nucleic acid probe detectable by specific nucleic acid binding protein
US5026840A (en) * 1985-01-10 1991-06-25 Molecular Diagnostics, Inc. Photochemical nucleic acid-labeling reagent having a polyalklamine spacer
US4957870A (en) * 1985-11-01 1990-09-18 Becton, Dickinson And Company Detection of Reticulocytes, RNA and DNA
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US5242797A (en) * 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
US5047321A (en) * 1988-06-15 1991-09-10 Becton Dickinson & Co. Method for analysis of cellular components of a fluid
US4937198A (en) * 1989-07-28 1990-06-26 Becton, Dickinson And Company Novel fluorescent dye
US5049490A (en) * 1990-02-20 1991-09-17 Eastman Kodak Co. Quantitative determination of a DNA polymerase and a test kit useful in same
US5312921A (en) * 1990-03-14 1994-05-17 Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
WO1991013897A1 (en) * 1990-03-14 1991-09-19 The Regents Of The University Of California Multichromophore fluorescent probes
JP2985446B2 (ja) * 1990-10-31 1999-11-29 東ソー株式会社 核酸の検出及び測定方法
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
JPH0540097A (ja) * 1991-08-06 1993-02-19 Hitachi Chem Co Ltd 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬
US5316906A (en) * 1991-08-23 1994-05-31 Molecular Probes, Inc. Enzymatic analysis using substrates that yield fluorescent precipitates
ATE152831T1 (de) * 1991-09-16 1997-05-15 Molecular Probes Inc Dimere unsymmetrische cyaninfarbstoffe
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain

Also Published As

Publication number Publication date
KR950032641A (ko) 1995-12-22
DE69533660T2 (de) 2005-11-10
CA2148140C (en) 2010-08-10
EP0684316B1 (de) 2004-10-20
KR100388732B1 (ko) 2004-09-04
US5563037A (en) 1996-10-08
EP0684316A1 (de) 1995-11-29
CN1100884C (zh) 2003-02-05
JPH0889299A (ja) 1996-04-09
CN1114359A (zh) 1996-01-03
JP4260903B2 (ja) 2009-04-30
CA2148140A1 (en) 1995-10-30
DE69533660D1 (de) 2004-11-25

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