ATE280243T1 - Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits - Google Patents
Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kitsInfo
- Publication number
- ATE280243T1 ATE280243T1 AT95302921T AT95302921T ATE280243T1 AT E280243 T1 ATE280243 T1 AT E280243T1 AT 95302921 T AT95302921 T AT 95302921T AT 95302921 T AT95302921 T AT 95302921T AT E280243 T1 ATE280243 T1 AT E280243T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- fluorescent dyes
- double strand
- strand nucleic
- detecting double
- Prior art date
Links
- 239000007850 fluorescent dye Substances 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Plural Heterocyclic Compounds (AREA)
- Luminescent Compositions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23539694A | 1994-04-29 | 1994-04-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE280243T1 true ATE280243T1 (de) | 2004-11-15 |
Family
ID=22885321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95302921T ATE280243T1 (de) | 1994-04-29 | 1995-04-28 | Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5563037A (de) |
| EP (1) | EP0684316B1 (de) |
| JP (1) | JP4260903B2 (de) |
| KR (1) | KR100388732B1 (de) |
| CN (1) | CN1100884C (de) |
| AT (1) | ATE280243T1 (de) |
| CA (1) | CA2148140C (de) |
| DE (1) | DE69533660T2 (de) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5935522A (en) * | 1990-06-04 | 1999-08-10 | University Of Utah Research Foundation | On-line DNA analysis system with rapid thermal cycling |
| US7273749B1 (en) | 1990-06-04 | 2007-09-25 | University Of Utah Research Foundation | Container for carrying out and monitoring biological processes |
| US7081226B1 (en) | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
| US6180403B1 (en) * | 1999-10-28 | 2001-01-30 | Isis Pharmaceuticals Inc. | Antisense inhibition of tumor necrosis factor alpha converting enzyme (TACE) expression |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| JP2001501454A (ja) * | 1996-05-17 | 2001-02-06 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | プライマーに制御された増幅産物の蛍光検出による標的細菌の同定 |
| SE506700C2 (sv) * | 1996-05-31 | 1998-02-02 | Mikael Kubista | Sond och förfaranden för analys av nukleinsyra |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| US6312930B1 (en) | 1996-09-16 | 2001-11-06 | E. I. Du Pont De Nemours And Company | Method for detecting bacteria using PCR |
| WO1998036096A1 (en) * | 1997-02-14 | 1998-08-20 | E.I. Du Pont De Nemours And Company | Detection of double-stranded dna in a homogeneous solution |
| EP2316571A3 (de) | 1998-05-01 | 2011-07-27 | Gen-Probe Incorporated | Automatisches Diagnoseanalysegerät und Verfahren |
| US6242188B1 (en) | 1999-07-30 | 2001-06-05 | Applied Gene Technologies, Inc. | Sample processing to release nucleic acids for direct detection |
| DE60042370D1 (de) | 1999-12-21 | 2009-07-23 | Ortho Clinical Diagnostics Inc | Detektion von nukleinsäuren |
| US7354707B2 (en) | 2000-06-27 | 2008-04-08 | National Institute Of Advanced Industrial Science And Technology | Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method |
| WO2002014548A1 (en) * | 2000-08-10 | 2002-02-21 | Applied Gene Technologies, Inc. | Compositions and methods for nucleic acids sample processing and amplification |
| US6573051B2 (en) * | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| US7267945B2 (en) * | 2001-03-26 | 2007-09-11 | Applera Corporation | Methods of determining the presence of polynucleotides employing amplification |
| US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US6977148B2 (en) * | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| US7297485B2 (en) * | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| ES2301754T3 (es) * | 2002-02-12 | 2008-07-01 | University Of Utah Research Foundation | Analisis de pruebas multiples de amplificaciones de acidos nucleicos en tiempo real. |
| AU2003288906C1 (en) * | 2002-09-20 | 2010-12-09 | Yale University | Riboswitches, methods for their use, and compositions for use with riboswitches. |
| ES2438967T3 (es) * | 2002-10-23 | 2014-01-21 | University Of Utah Research Foundation | Análisis de fusión de amplicones con colorantes de saturación |
| US9850381B2 (en) * | 2002-10-23 | 2017-12-26 | University Of Utah Research Foundation | Amplicon melting analysis with saturation dyes |
| AU2003301825A1 (en) * | 2002-10-31 | 2004-06-07 | Pfizer Products, Inc. | Panels of molecular targets differentially expressed during cd8+ t-cell priming |
| US20040191803A1 (en) * | 2002-11-22 | 2004-09-30 | Michela Gallagher | Target for therapy of cognitive impairment |
| US20040121338A1 (en) * | 2002-12-19 | 2004-06-24 | Alsmadi Osama A. | Real-time detection of rolling circle amplification products |
| EP2261371B1 (de) | 2002-12-20 | 2015-07-22 | QIAGEN GmbH | Nukleinsäureamplifikation |
| US7955795B2 (en) * | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US6977153B2 (en) * | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| ATE355529T1 (de) * | 2003-08-20 | 2006-03-15 | Sysmex Corp | Probenanalysegerät und eine vorrichtung zur detektion von nukleinsäuren |
| KR100906749B1 (ko) * | 2004-03-25 | 2009-07-09 | (주)바이오니아 | 인터컬레이팅 형광염료가 표지된 프로브를 이용한 핵산 증폭 측정 방법 |
| US9657347B2 (en) | 2004-04-20 | 2017-05-23 | University of Utah Research Foundation and BioFire Defense, LLC | Nucleic acid melting analysis with saturation dyes |
| US7387887B2 (en) * | 2004-04-20 | 2008-06-17 | University Of Utah Research Foundation | Nucleic acid melting analysis with saturation dyes |
| US7776567B2 (en) | 2005-03-17 | 2010-08-17 | Biotium, Inc. | Dimeric and trimeric nucleic acid dyes, and associated systems and methods |
| EP1863908B1 (de) | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
| US20060275798A1 (en) * | 2005-04-04 | 2006-12-07 | Steichen John C | Detection of organisms using a media sachet and primer directed nucleic acid amplification |
| US7456281B2 (en) * | 2005-04-20 | 2008-11-25 | Idaho Technology, Inc. | Nucleic acid melting analysis with saturation dyes |
| US20060246513A1 (en) * | 2005-05-02 | 2006-11-02 | Bohannon Robert C | Method and device to detect the presence of analytes in a sample |
| EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
| US7781165B2 (en) * | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| US8313901B2 (en) * | 2005-12-21 | 2012-11-20 | Yale University | Methods and compositions related to the modulation of riboswitches |
| US20080057499A1 (en) * | 2006-02-06 | 2008-03-06 | Affymetrix, Inc. | Methods for high specificity whole genome amplification and hybridization |
| JP2010502752A (ja) * | 2006-09-11 | 2010-01-28 | イェール ユニバーシティー | リシンリボスイッチ、リシンリボスイッチを用いた構造に基づく化合物設計、ならびにリシンリボスイッチを用いた使用のための方法および組成物 |
| CN101801185A (zh) | 2007-03-22 | 2010-08-11 | 耶鲁大学 | 与控制可变剪接的核糖开关有关的方法和组合物 |
| JP2010528616A (ja) * | 2007-05-29 | 2010-08-26 | イェール ユニバーシティー | オルタナティブスプライシングとrnaのスプライシングを調節するリボスイッチに関連する方法と組成物 |
| WO2008156987A2 (en) | 2007-05-29 | 2008-12-24 | Yale University | Riboswitches and methods and compositions for use of and with riboswitches |
| US20100285473A1 (en) | 2009-01-27 | 2010-11-11 | John Wolff | Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection |
| EP2430161A1 (de) | 2009-05-15 | 2012-03-21 | Yale University | Gemm-riboswitches, strukturbasierte verbindungskonstruktion mit gemm-riboswitches sowie verfahren und zusammensetzungen zur verwendung von und mit gemm-riboswitches |
| US8877437B1 (en) | 2009-12-23 | 2014-11-04 | Biotium, Inc. | Methods of using dyes in association with nucleic acid staining or detection |
| US20120321647A1 (en) | 2010-01-12 | 2012-12-20 | Yale University | Structured rna motifs and compounds and methods for their use |
| US8628914B2 (en) | 2010-05-26 | 2014-01-14 | Qiagen Gaithersburg, Inc. | Quantitative helicase assay |
| EP2754496A3 (de) | 2010-07-23 | 2017-08-02 | Beckman Coulter, Inc. | System oder Verfahren zur Aufnahme analytischer Einheiten |
| WO2012021554A1 (en) | 2010-08-09 | 2012-02-16 | Yale University | Cyclic di-gmp-ii riboswitches, motifs, and compounds, and methods for their use |
| CN102095705A (zh) * | 2010-12-09 | 2011-06-15 | 山东理工大学 | 一种基于激光扫描共聚焦显微镜的头发丝成像方法 |
| US9580713B2 (en) | 2011-09-17 | 2017-02-28 | Yale University | Fluoride-responsive riboswitches, fluoride transporters, and methods of use |
| BR112014011048A2 (pt) | 2011-11-07 | 2017-05-02 | Beckman Coulter Inc | braço robótico |
| KR20140091033A (ko) | 2011-11-07 | 2014-07-18 | 베크만 컬터, 인코포레이티드 | 검체 컨테이너 검출 |
| ES2729283T3 (es) | 2011-11-07 | 2019-10-31 | Beckman Coulter Inc | Sistema de centrífuga y flujo de trabajo |
| EP3373015A1 (de) | 2011-11-07 | 2018-09-12 | Beckman Coulter Inc. | Aliquotierungssystem und arbeitsablauf |
| BR112014010955A2 (pt) | 2011-11-07 | 2017-06-06 | Beckman Coulter Inc | sistema e método para processar amostras |
| EP2776847A1 (de) | 2011-11-07 | 2014-09-17 | Beckman Coulter, Inc. | Magnetische dämpfung für probentransportsystem |
| WO2014152775A2 (en) | 2013-03-14 | 2014-09-25 | Certirx Corporation | Nucleic acid-based authentication and identification codes |
| CN110426442B (zh) * | 2016-06-13 | 2022-02-11 | 苏州优逸兰迪生物科技有限公司 | 一种核酸凝胶电泳测试的方法 |
| US10427162B2 (en) | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
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| US4257774A (en) * | 1979-07-16 | 1981-03-24 | Meloy Laboratories, Inc. | Intercalation inhibition assay for compounds that interact with DNA or RNA |
| US4544546A (en) * | 1980-04-21 | 1985-10-01 | Abbott Laboratories | Fluorescent nucleic acid stains |
| CA1155041A (en) * | 1980-04-21 | 1983-10-11 | Michael E. Jolley | Fluorescent nucleic acid stains |
| US4777129A (en) * | 1983-12-12 | 1988-10-11 | Molecular Diagnostics, Inc. | Nucleic acid probe detectable by specific nucleic acid binding protein |
| US5026840A (en) * | 1985-01-10 | 1991-06-25 | Molecular Diagnostics, Inc. | Photochemical nucleic acid-labeling reagent having a polyalklamine spacer |
| US4957870A (en) * | 1985-11-01 | 1990-09-18 | Becton, Dickinson And Company | Detection of Reticulocytes, RNA and DNA |
| US4883867A (en) * | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| US5242797A (en) * | 1986-03-21 | 1993-09-07 | Myron J. Block | Nucleic acid assay method |
| US5047321A (en) * | 1988-06-15 | 1991-09-10 | Becton Dickinson & Co. | Method for analysis of cellular components of a fluid |
| US4937198A (en) * | 1989-07-28 | 1990-06-26 | Becton, Dickinson And Company | Novel fluorescent dye |
| US5049490A (en) * | 1990-02-20 | 1991-09-17 | Eastman Kodak Co. | Quantitative determination of a DNA polymerase and a test kit useful in same |
| US5312921A (en) * | 1990-03-14 | 1994-05-17 | Regents Of The University Of California | Dyes designed for high sensitivity detection of double-stranded DNA |
| WO1991013897A1 (en) * | 1990-03-14 | 1991-09-19 | The Regents Of The University Of California | Multichromophore fluorescent probes |
| JP2985446B2 (ja) * | 1990-10-31 | 1999-11-29 | 東ソー株式会社 | 核酸の検出及び測定方法 |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| JPH0540097A (ja) * | 1991-08-06 | 1993-02-19 | Hitachi Chem Co Ltd | 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬 |
| US5316906A (en) * | 1991-08-23 | 1994-05-31 | Molecular Probes, Inc. | Enzymatic analysis using substrates that yield fluorescent precipitates |
| ATE152831T1 (de) * | 1991-09-16 | 1997-05-15 | Molecular Probes Inc | Dimere unsymmetrische cyaninfarbstoffe |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
-
1995
- 1995-04-28 AT AT95302921T patent/ATE280243T1/de not_active IP Right Cessation
- 1995-04-28 DE DE69533660T patent/DE69533660T2/de not_active Expired - Lifetime
- 1995-04-28 CA CA2148140A patent/CA2148140C/en not_active Expired - Lifetime
- 1995-04-28 EP EP95302921A patent/EP0684316B1/de not_active Expired - Lifetime
- 1995-04-29 CN CN95106515A patent/CN1100884C/zh not_active Expired - Lifetime
- 1995-04-29 KR KR1019950010417A patent/KR100388732B1/ko not_active Expired - Fee Related
- 1995-05-01 JP JP10746895A patent/JP4260903B2/ja not_active Expired - Fee Related
- 1995-12-21 US US08/576,861 patent/US5563037A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| KR950032641A (ko) | 1995-12-22 |
| DE69533660T2 (de) | 2005-11-10 |
| CA2148140C (en) | 2010-08-10 |
| EP0684316B1 (de) | 2004-10-20 |
| KR100388732B1 (ko) | 2004-09-04 |
| US5563037A (en) | 1996-10-08 |
| EP0684316A1 (de) | 1995-11-29 |
| CN1100884C (zh) | 2003-02-05 |
| JPH0889299A (ja) | 1996-04-09 |
| CN1114359A (zh) | 1996-01-03 |
| JP4260903B2 (ja) | 2009-04-30 |
| CA2148140A1 (en) | 1995-10-30 |
| DE69533660D1 (de) | 2004-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |