ATE326550T1 - Quantitative methode zum nachweis der nukleotidkonzentration - Google Patents
Quantitative methode zum nachweis der nukleotidkonzentrationInfo
- Publication number
- ATE326550T1 ATE326550T1 AT99918228T AT99918228T ATE326550T1 AT E326550 T1 ATE326550 T1 AT E326550T1 AT 99918228 T AT99918228 T AT 99918228T AT 99918228 T AT99918228 T AT 99918228T AT E326550 T1 ATE326550 T1 AT E326550T1
- Authority
- AT
- Austria
- Prior art keywords
- concentration
- quantitative method
- nucleotide concentration
- detecting nucleotide
- detection
- Prior art date
Links
- 239000002773 nucleotide Substances 0.000 title 1
- 238000004445 quantitative analysis Methods 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 3
- 238000001514 detection method Methods 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000002787 antisense oligonuctleotide Substances 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 238000001499 laser induced fluorescence spectroscopy Methods 0.000 abstract 1
- 238000003908 quality control method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/083,837 US6251591B1 (en) | 1997-02-27 | 1998-05-22 | Quantitative method for detecting nucleotide concentration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE326550T1 true ATE326550T1 (de) | 2006-06-15 |
Family
ID=22181015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99918228T ATE326550T1 (de) | 1998-05-22 | 1999-05-20 | Quantitative methode zum nachweis der nukleotidkonzentration |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6251591B1 (de) |
| EP (1) | EP1078100B1 (de) |
| AT (1) | ATE326550T1 (de) |
| AU (1) | AU3624199A (de) |
| DE (1) | DE69931365D1 (de) |
| WO (1) | WO1999061664A1 (de) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6656692B2 (en) | 1999-12-21 | 2003-12-02 | Ingeneus Corporation | Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes |
| US6403313B1 (en) | 1999-12-21 | 2002-06-11 | Ingeneus Corporation | Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators |
| US6420115B1 (en) | 1999-12-21 | 2002-07-16 | Ingeneus Corporation | Cation mediated triplex hybridization assay |
| US6858390B2 (en) | 1998-12-31 | 2005-02-22 | Ingeneus Corporation | Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes |
| US7309569B2 (en) | 1999-12-21 | 2007-12-18 | Ingeneus, Inc. | Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes |
| US6927027B2 (en) | 1999-12-21 | 2005-08-09 | Ingeneus Corporation | Nucleic acid multiplex formation |
| US6924108B2 (en) | 1999-12-21 | 2005-08-02 | Ingeneus Corporation | Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues |
| US6911536B1 (en) | 1999-12-21 | 2005-06-28 | Ingeneus Corporation | Triplex and quadruplex catalytic hybridization |
| US20030181412A1 (en) * | 1999-12-21 | 2003-09-25 | Ingeneus Corporation | Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses |
| US7052844B2 (en) * | 1999-12-21 | 2006-05-30 | Ingeneus, Inc. | Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism |
| US20030170659A1 (en) * | 2000-01-24 | 2003-09-11 | Ingeneus Corporation | Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding |
| US7220541B2 (en) | 2000-01-24 | 2007-05-22 | Ingeneus, Inc. | Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions |
| US6982147B2 (en) * | 2000-01-24 | 2006-01-03 | Ingeneus Corporation | Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions |
| US6613524B1 (en) | 2000-01-24 | 2003-09-02 | Ingeneus Corporation | Amperometric affinity assay and electrically stimulated complexes of nucleic acids |
| US7593819B2 (en) * | 2001-07-11 | 2009-09-22 | Applied Biosystems, Llc | Internal calibration standards for electrophoretic analyses |
| DE10221004A1 (de) * | 2002-05-11 | 2003-12-11 | Friz Biochem Gmbh | Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen unter Verwendung im wesentlichen ungeladener Ligat-Oligonukleotide |
| DE10221091B4 (de) * | 2002-05-11 | 2005-12-15 | Friz Biochem Gesellschaft Für Bioanalytik Mbh | SECM zur Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen |
| US20040180345A1 (en) * | 2003-03-14 | 2004-09-16 | Ingeneus Corporation | Pre-incubation method to improve signal/noise ratio of nucleic acid assays |
| RU2464320C2 (ru) * | 2004-09-24 | 2012-10-20 | Инджиниес Инк. | Геномный анализ |
| US10253353B2 (en) * | 2013-12-06 | 2019-04-09 | The Broad Institute, Inc. | Enhanced methods of ribonucleic acid hybridization |
| CN107365854A (zh) * | 2017-08-16 | 2017-11-21 | 厦门智因互联科技有限公司 | 一种tp53基因snp位点多重实时荧光pcr检测引物、探针及其试剂盒 |
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| US4220450A (en) | 1978-04-05 | 1980-09-02 | Syva Company | Chemically induced fluorescence immunoassay |
| US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5166330A (en) | 1983-09-06 | 1992-11-24 | Hoechst Aktiengesellschaft | Process for the preparation of nucleoside alkyl-aralkyl- and aryl-phosphonites and -phosphonates |
| FR2558172B1 (fr) | 1984-01-16 | 1986-06-13 | Inst Nat Sante Rech Med | Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue |
| US5470974A (en) | 1985-03-15 | 1995-11-28 | Neu-Gene Development Group | Uncharged polynucleotide-binding polymers |
| US5405938A (en) | 1989-12-20 | 1995-04-11 | Anti-Gene Development Group | Sequence-specific binding polymers for duplex nucleic acids |
| US5166315A (en) | 1989-12-20 | 1992-11-24 | Anti-Gene Development Group | Sequence-specific binding polymers for duplex nucleic acids |
| US5217866A (en) | 1985-03-15 | 1993-06-08 | Anti-Gene Development Group | Polynucleotide assay reagent and method |
| US5521063A (en) | 1985-03-15 | 1996-05-28 | Antivirals Inc. | Polynucleotide reagent containing chiral subunits and methods of use |
| JP2528107B2 (ja) | 1985-03-15 | 1996-08-28 | サマ−トン,ジエ−ムス | ポリヌクレオチド測定試薬と方法 |
| US5217592A (en) | 1985-09-26 | 1993-06-08 | Jones Kenneth W | Electrophoresis and vacuum molecular transfer apparatus |
| US5501949A (en) | 1985-12-10 | 1996-03-26 | Murex Diagnostics Corporation | Particle bound binding component immunoassay |
| DE3785591T2 (de) | 1986-01-10 | 1993-09-02 | Amoco Corp | Kompetitiver homogener test. |
| US5310650A (en) | 1986-09-29 | 1994-05-10 | Abbott Laboratoires | Method and device for improved reaction kinetics in nucleic acid hybridizations |
| US4787963A (en) | 1987-05-04 | 1988-11-29 | Syntro Corporation | Method and means for annealing complementary nucleic acid molecules at an accelerated rate |
| US5100775A (en) | 1988-03-16 | 1992-03-31 | Smyczek Peter J | Method for conducting nucleic acid hybridization in chamber with precise fluid delivery |
| CA1337639C (en) | 1989-08-01 | 1995-11-28 | Joseph Eugene Celebuski | Dna probe assay using neutrally charged probe strands |
| US5623065A (en) | 1990-08-13 | 1997-04-22 | Isis Pharmaceuticals, Inc. | Gapped 2' modified oligonucleotides |
| US5459255A (en) | 1990-01-11 | 1995-10-17 | Isis Pharmaceuticals, Inc. | N-2 substituted purines |
| US5618704A (en) | 1990-07-27 | 1997-04-08 | Isis Pharmacueticals, Inc. | Backbone-modified oligonucleotide analogs and preparation thereof through radical coupling |
| US5610289A (en) | 1990-07-27 | 1997-03-11 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogues |
| US5541307A (en) | 1990-07-27 | 1996-07-30 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs and solid phase synthesis thereof |
| US5677437A (en) | 1990-07-27 | 1997-10-14 | Isis Pharmaceuticals, Inc. | Heteroatomic oligonucleoside linkages |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5223618A (en) | 1990-08-13 | 1993-06-29 | Isis Pharmaceuticals, Inc. | 4'-desmethyl nucleoside analog compounds |
| EP0533906A4 (en) | 1991-04-11 | 1994-09-28 | Baxter Diagnostics Inc | Detection of dna/rna by fluorescence polarization |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5632957A (en) | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
| US5332659A (en) | 1992-04-09 | 1994-07-26 | The United States Of America As Represented By The Secretary Of The Navy | Light emission-or absorbance-based binding assays for polynucleic acids |
| DE4216949C2 (de) | 1992-05-22 | 1997-07-24 | Christoph Prof Dr Dr Cremer | Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben |
| US5674698A (en) | 1992-09-14 | 1997-10-07 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| US5503980A (en) | 1992-11-06 | 1996-04-02 | Trustees Of Boston University | Positional sequencing by hybridization |
| US5445935A (en) | 1992-11-23 | 1995-08-29 | Royer; Catherine A. | Quantitative detection of macromolecules with fluorescent oligonucleotides |
| DE69333991T2 (de) | 1992-11-27 | 2006-12-14 | Canon K.K. | Verfahren und Sonde zum Nachweis von Nukleinsäuren |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5723288A (en) | 1994-05-06 | 1998-03-03 | The University Of North Carolina At Chapel Hill | Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans, and kits useful therefor |
| US5747247A (en) | 1994-07-25 | 1998-05-05 | The Regents Of The University Of California | Spectroscopic helicase assay |
| DE69610304T2 (de) | 1995-05-05 | 2001-04-05 | Perkin-Elmer Corp., Foster City | Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay |
| DE69634696T2 (de) | 1995-10-06 | 2006-01-19 | PerSeptive Biosystems, Inc., Framingham | Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden |
| CA2190430A1 (en) | 1995-12-12 | 1997-06-13 | Margret Barbara Basinski | Method for measuring genetic messages |
| US5824557A (en) | 1996-04-02 | 1998-10-20 | Panvera Corporation | Method for detecting and quantitating nucleic acid impurities in biochemical preparations |
| ES2202821T3 (es) | 1997-02-27 | 2004-04-01 | Ingeneus Corp. | Analisis de nucleotidos en disolucion usando sondas de acidos nucleicos peptidos. |
| US6060242A (en) * | 1997-02-27 | 2000-05-09 | Lorne Park Research, Inc. | PNA diagnostic methods |
| US5846729A (en) * | 1997-02-27 | 1998-12-08 | Lorne Park Research, Inc. | Assaying nucleotides in solution using a fluorescent intensity quenching effect |
-
1998
- 1998-05-22 US US09/083,837 patent/US6251591B1/en not_active Expired - Fee Related
-
1999
- 1999-05-20 EP EP99918228A patent/EP1078100B1/de not_active Expired - Lifetime
- 1999-05-20 WO PCT/IB1999/000904 patent/WO1999061664A1/en not_active Ceased
- 1999-05-20 AU AU36241/99A patent/AU3624199A/en not_active Abandoned
- 1999-05-20 DE DE69931365T patent/DE69931365D1/de not_active Expired - Fee Related
- 1999-05-20 AT AT99918228T patent/ATE326550T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US6251591B1 (en) | 2001-06-26 |
| DE69931365D1 (de) | 2006-06-22 |
| AU3624199A (en) | 1999-12-13 |
| EP1078100B1 (de) | 2006-05-17 |
| WO1999061664A1 (en) | 1999-12-02 |
| EP1078100A1 (de) | 2001-02-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |