ATE326550T1 - Quantitative methode zum nachweis der nukleotidkonzentration - Google Patents

Quantitative methode zum nachweis der nukleotidkonzentration

Info

Publication number
ATE326550T1
ATE326550T1 AT99918228T AT99918228T ATE326550T1 AT E326550 T1 ATE326550 T1 AT E326550T1 AT 99918228 T AT99918228 T AT 99918228T AT 99918228 T AT99918228 T AT 99918228T AT E326550 T1 ATE326550 T1 AT E326550T1
Authority
AT
Austria
Prior art keywords
concentration
quantitative method
nucleotide concentration
detecting nucleotide
detection
Prior art date
Application number
AT99918228T
Other languages
English (en)
Inventor
Eileen Xiao-Feng Nie
Yuan Min Wu
Original Assignee
Lorne Park Res Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lorne Park Res Inc filed Critical Lorne Park Res Inc
Application granted granted Critical
Publication of ATE326550T1 publication Critical patent/ATE326550T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99918228T 1998-05-22 1999-05-20 Quantitative methode zum nachweis der nukleotidkonzentration ATE326550T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/083,837 US6251591B1 (en) 1997-02-27 1998-05-22 Quantitative method for detecting nucleotide concentration

Publications (1)

Publication Number Publication Date
ATE326550T1 true ATE326550T1 (de) 2006-06-15

Family

ID=22181015

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99918228T ATE326550T1 (de) 1998-05-22 1999-05-20 Quantitative methode zum nachweis der nukleotidkonzentration

Country Status (6)

Country Link
US (1) US6251591B1 (de)
EP (1) EP1078100B1 (de)
AT (1) ATE326550T1 (de)
AU (1) AU3624199A (de)
DE (1) DE69931365D1 (de)
WO (1) WO1999061664A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6403313B1 (en) 1999-12-21 2002-06-11 Ingeneus Corporation Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators
US6656692B2 (en) 1999-12-21 2003-12-02 Ingeneus Corporation Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6420115B1 (en) 1999-12-21 2002-07-16 Ingeneus Corporation Cation mediated triplex hybridization assay
US6858390B2 (en) 1998-12-31 2005-02-22 Ingeneus Corporation Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes
US20030181412A1 (en) * 1999-12-21 2003-09-25 Ingeneus Corporation Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses
US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US6924108B2 (en) 1999-12-21 2005-08-02 Ingeneus Corporation Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues
US7309569B2 (en) 1999-12-21 2007-12-18 Ingeneus, Inc. Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes
US6911536B1 (en) 1999-12-21 2005-06-28 Ingeneus Corporation Triplex and quadruplex catalytic hybridization
US6927027B2 (en) 1999-12-21 2005-08-09 Ingeneus Corporation Nucleic acid multiplex formation
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6613524B1 (en) 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US7220541B2 (en) 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
EP1404878A4 (de) * 2001-07-11 2006-12-06 Applera Corp Interne kalibrierungsstandards für elektrophoretische analysen
DE10221004A1 (de) * 2002-05-11 2003-12-11 Friz Biochem Gmbh Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen unter Verwendung im wesentlichen ungeladener Ligat-Oligonukleotide
DE10221091B4 (de) * 2002-05-11 2005-12-15 Friz Biochem Gesellschaft Für Bioanalytik Mbh SECM zur Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
KR20070062575A (ko) * 2004-09-24 2007-06-15 인제네우스 인크. 게놈 분석
WO2015085194A1 (en) * 2013-12-06 2015-06-11 The Broad Institute, Inc. Enhanced methods of ribonucleic acid hybridization
CN107365854A (zh) * 2017-08-16 2017-11-21 厦门智因互联科技有限公司 一种tp53基因snp位点多重实时荧光pcr检测引物、探针及其试剂盒

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4220450A (en) 1978-04-05 1980-09-02 Syva Company Chemically induced fluorescence immunoassay
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5166330A (en) 1983-09-06 1992-11-24 Hoechst Aktiengesellschaft Process for the preparation of nucleoside alkyl-aralkyl- and aryl-phosphonites and -phosphonates
FR2558172B1 (fr) 1984-01-16 1986-06-13 Inst Nat Sante Rech Med Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue
US5405938A (en) 1989-12-20 1995-04-11 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5166315A (en) 1989-12-20 1992-11-24 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5470974A (en) 1985-03-15 1995-11-28 Neu-Gene Development Group Uncharged polynucleotide-binding polymers
US5521063A (en) 1985-03-15 1996-05-28 Antivirals Inc. Polynucleotide reagent containing chiral subunits and methods of use
US5217866A (en) 1985-03-15 1993-06-08 Anti-Gene Development Group Polynucleotide assay reagent and method
JP2528107B2 (ja) 1985-03-15 1996-08-28 サマ−トン,ジエ−ムス ポリヌクレオチド測定試薬と方法
US5217592A (en) 1985-09-26 1993-06-08 Jones Kenneth W Electrophoresis and vacuum molecular transfer apparatus
US5501949A (en) 1985-12-10 1996-03-26 Murex Diagnostics Corporation Particle bound binding component immunoassay
EP0232967B1 (de) 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
US5310650A (en) 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
US4787963A (en) 1987-05-04 1988-11-29 Syntro Corporation Method and means for annealing complementary nucleic acid molecules at an accelerated rate
US5100775A (en) 1988-03-16 1992-03-31 Smyczek Peter J Method for conducting nucleic acid hybridization in chamber with precise fluid delivery
CA1337639C (en) 1989-08-01 1995-11-28 Joseph Eugene Celebuski Dna probe assay using neutrally charged probe strands
US5623065A (en) 1990-08-13 1997-04-22 Isis Pharmaceuticals, Inc. Gapped 2' modified oligonucleotides
US5459255A (en) 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
US5618704A (en) 1990-07-27 1997-04-08 Isis Pharmacueticals, Inc. Backbone-modified oligonucleotide analogs and preparation thereof through radical coupling
US5223618A (en) 1990-08-13 1993-06-29 Isis Pharmaceuticals, Inc. 4'-desmethyl nucleoside analog compounds
US5541307A (en) 1990-07-27 1996-07-30 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs and solid phase synthesis thereof
US5610289A (en) 1990-07-27 1997-03-11 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogues
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5677437A (en) 1990-07-27 1997-10-14 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
JPH05508323A (ja) 1991-04-11 1993-11-25 デイド、ベーリング、インコーポレイテッド 蛍光分極によるdna/rnaの検出
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
US5332659A (en) 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
DE4216949C2 (de) 1992-05-22 1997-07-24 Christoph Prof Dr Dr Cremer Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben
US5674698A (en) 1992-09-14 1997-10-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5445935A (en) 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
DE69333991T2 (de) 1992-11-27 2006-12-14 Canon K.K. Verfahren und Sonde zum Nachweis von Nukleinsäuren
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5723288A (en) 1994-05-06 1998-03-03 The University Of North Carolina At Chapel Hill Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans, and kits useful therefor
US5747247A (en) 1994-07-25 1998-05-05 The Regents Of The University Of California Spectroscopic helicase assay
ATE196322T1 (de) 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
WO1997012995A1 (en) 1995-10-06 1997-04-10 Perseptive Biosystems, Inc. Methods and kit for hybridization analysis using peptide nucleic acid probes
CA2190430A1 (en) 1995-12-12 1997-06-13 Margret Barbara Basinski Method for measuring genetic messages
US5824557A (en) 1996-04-02 1998-10-20 Panvera Corporation Method for detecting and quantitating nucleic acid impurities in biochemical preparations
US5846729A (en) * 1997-02-27 1998-12-08 Lorne Park Research, Inc. Assaying nucleotides in solution using a fluorescent intensity quenching effect
US6060242A (en) * 1997-02-27 2000-05-09 Lorne Park Research, Inc. PNA diagnostic methods
JP4110223B2 (ja) 1997-02-27 2008-07-02 インジェネウス・インコーポレーテッド Pnaプローブを用いる、溶液中でのヌクレオチドアッセイ法

Also Published As

Publication number Publication date
WO1999061664A1 (en) 1999-12-02
US6251591B1 (en) 2001-06-26
AU3624199A (en) 1999-12-13
EP1078100A1 (de) 2001-02-28
DE69931365D1 (de) 2006-06-22
EP1078100B1 (de) 2006-05-17

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