ES2052607T3 - Metodos y kits para detectar secuencias de acidos nucleicos. - Google Patents
Metodos y kits para detectar secuencias de acidos nucleicos.Info
- Publication number
- ES2052607T3 ES2052607T3 ES88100162T ES88100162T ES2052607T3 ES 2052607 T3 ES2052607 T3 ES 2052607T3 ES 88100162 T ES88100162 T ES 88100162T ES 88100162 T ES88100162 T ES 88100162T ES 2052607 T3 ES2052607 T3 ES 2052607T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- methods
- kits
- acid sequences
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
LA INVENCON PROPORCIONA METODOS Y COMPOSICIONES PARA UN ENSAYO CUANTITATIVO RAPIDO DE HIBRIDACION DE ACIDO NUCLEICO PARA DETECTAR UNA SECUENCIA DE ADN O ARN ENUNA MUESTRA BIOLOGICA. EL METODO IMPLICA LA HIBRIDACION EN SOLUCION DE UNA SECUENCIA DE ACIDO NUCLEICO DIANA CON UNA SONDA MARCADA DE MANERA DETECTABLE, SEPARACION DE LA SONDA DE ACIDO NUCLEICO DIANA DE LA SONDA NO HIBRIDIZADA POR CROMATOGRAFIA DE EXCLUSION SOBRE GEL O DE AFINIDAD, Y DETECCION DE LA CANTIDAD DE SONDA MARCADA COMO INDICACION DEL ACIDO NUCLEICO DIANA PRESENTE EN LA MUESTRA BIOLOGICA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US283887A | 1987-01-09 | 1987-01-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2052607T3 true ES2052607T3 (es) | 1994-07-16 |
Family
ID=21702766
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES88100162T Expired - Lifetime ES2052607T3 (es) | 1987-01-09 | 1988-01-08 | Metodos y kits para detectar secuencias de acidos nucleicos. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5981171A (es) |
EP (1) | EP0278220B1 (es) |
JP (1) | JP2690738B2 (es) |
AT (1) | ATE82773T1 (es) |
AU (2) | AU619170B2 (es) |
CA (1) | CA1340840C (es) |
DE (1) | DE3876108T2 (es) |
ES (1) | ES2052607T3 (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5310650A (en) * | 1986-09-29 | 1994-05-10 | Abbott Laboratoires | Method and device for improved reaction kinetics in nucleic acid hybridizations |
JP2549530B2 (ja) * | 1987-10-01 | 1996-10-30 | 新技術事業団 | 非放射性の核酸標識法及びこれを使用した検出法 |
CA1325761C (en) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Method of detecting an intended nucleic acid in a sample |
GB8827160D0 (en) * | 1988-11-21 | 1988-12-29 | Apothekernes Lab | Detection & quantitative determination of rna & dna |
DE3916871A1 (de) * | 1989-05-24 | 1990-11-29 | Boehringer Mannheim Gmbh | Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren |
JPH0560761A (ja) * | 1991-08-30 | 1993-03-12 | Imuno Baion:Kk | 重合体の分析方法 |
US6100099A (en) | 1994-09-06 | 2000-08-08 | Abbott Laboratories | Test strip having a diagonal array of capture spots |
US6203978B1 (en) * | 1996-05-31 | 2001-03-20 | Du Vergier Ltd. | Capture of single stranded nucleic acids |
NL1010012C2 (nl) * | 1998-09-04 | 2000-03-07 | Alexander Adrianus Moen | Werkwijze voor het detecteren van nucleotidensequenties en/of nucleotide-bindende eiwitten. |
US6448422B1 (en) | 2000-07-03 | 2002-09-10 | Eli Lilly And Company | Chromogenic compound |
AU758744B2 (en) * | 2000-10-31 | 2003-03-27 | F. Hoffmann-La Roche Ag | Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain |
FI115139B (fi) * | 2001-01-10 | 2005-03-15 | Valtion Teknillinen | Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen |
ES2349949T3 (es) * | 2001-04-04 | 2011-01-13 | Wako Pure Chemical Industries, Ltd. | Electrofóresis con marcador de adn bicatenario. |
US6696254B2 (en) * | 2001-11-21 | 2004-02-24 | Kimberly-Clark Worldwide, Inc. | Detection and identification of enteric bacteria |
FI20021325A0 (fi) * | 2002-07-05 | 2002-07-05 | Valtion Teknillinen | Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi |
US7901883B2 (en) * | 2004-12-10 | 2011-03-08 | Genera Biosystems Limited | Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS) |
US20070042427A1 (en) * | 2005-05-03 | 2007-02-22 | Micronics, Inc. | Microfluidic laminar flow detection strip |
ES2403729T3 (es) * | 2007-04-05 | 2013-05-21 | Genera Biosystems Limited | Composiciones y métodos de detección |
CA2696433A1 (en) * | 2007-08-13 | 2009-03-12 | 3M Innovative Properties Company | Methods for detecting drug-resistant microbes |
WO2010048386A1 (en) * | 2008-10-24 | 2010-04-29 | Helicos Biosciences Corporation | Methods of sample preparation for nucleic acid analysis for nucleic acids available in limited amounts |
US8277651B2 (en) | 2009-03-13 | 2012-10-02 | Terrasep, Llc | Methods and apparatus for centrifugal liquid chromatography |
JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー. | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2422956A1 (fr) * | 1978-04-13 | 1979-11-09 | Pasteur Institut | Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede |
US4407942A (en) * | 1981-01-29 | 1983-10-04 | Atomic Energy Of Canada Limited | Fluorescent detection of DNA damage |
US4399303A (en) * | 1981-08-17 | 1983-08-16 | E. I. Du Pont De Nemours And Company | Preparation and use of nitroterephthalamic acids |
US4894228A (en) * | 1982-04-07 | 1990-01-16 | The United States Of America As Represented By The Department Of Health And Human Services | Vaccine against hepatitis A virus |
CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
WO1984003285A1 (en) * | 1983-02-22 | 1984-08-30 | Molecular Biosystems Inc | Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis |
IL71699A0 (en) * | 1983-05-02 | 1984-07-31 | Enzo Biochem Inc | Methods and kits for the analysis and detection of biological or genetic material |
CA1231303A (en) * | 1983-08-05 | 1988-01-12 | Robert J. Carrico | Detection of bacteria by nucleic acid hybridization |
FR2552879B1 (fr) * | 1983-10-03 | 1987-08-14 | Pasteur Institut | Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes |
US4599303A (en) * | 1983-12-12 | 1986-07-08 | Hri Associates, Inc. | Nucleic acid hybridization assay employing probes crosslinkable to target sequences |
GB8405437D0 (en) * | 1984-03-01 | 1984-04-04 | Amersham Int Plc | Detecting polynucleotide sequences |
US4766064A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay employing polyether and diagnostic kit |
US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
US4734363A (en) * | 1984-11-27 | 1988-03-29 | Molecular Diagnostics, Inc. | Large scale production of DNA probes |
US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
AU7015287A (en) * | 1986-03-19 | 1987-09-24 | Cetus Corporation | Detection of nucleic acid sequence using liquid hybridization method |
FR2596774B1 (fr) * | 1986-04-04 | 1988-07-22 | Pasteur Institut | Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants |
-
1988
- 1988-01-06 AU AU10106/88A patent/AU619170B2/en not_active Ceased
- 1988-01-08 AT AT88100162T patent/ATE82773T1/de not_active IP Right Cessation
- 1988-01-08 ES ES88100162T patent/ES2052607T3/es not_active Expired - Lifetime
- 1988-01-08 CA CA000556139A patent/CA1340840C/en not_active Expired - Fee Related
- 1988-01-08 DE DE8888100162T patent/DE3876108T2/de not_active Expired - Fee Related
- 1988-01-08 EP EP88100162A patent/EP0278220B1/en not_active Expired - Lifetime
- 1988-01-08 JP JP63003031A patent/JP2690738B2/ja not_active Expired - Fee Related
-
1990
- 1990-07-06 US US07/550,250 patent/US5981171A/en not_active Expired - Fee Related
-
1992
- 1992-02-07 AU AU10824/92A patent/AU630836B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
CA1340840C (en) | 1999-12-07 |
DE3876108D1 (de) | 1993-01-07 |
US5981171A (en) | 1999-11-09 |
ATE82773T1 (de) | 1992-12-15 |
JP2690738B2 (ja) | 1997-12-17 |
AU630836B2 (en) | 1992-11-05 |
EP0278220B1 (en) | 1992-11-25 |
AU619170B2 (en) | 1992-01-23 |
AU1082492A (en) | 1992-05-14 |
DE3876108T2 (de) | 1993-04-22 |
JPS63245698A (ja) | 1988-10-12 |
AU1010688A (en) | 1988-07-14 |
EP0278220A1 (en) | 1988-08-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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