ES2052607T3 - Metodos y kits para detectar secuencias de acidos nucleicos. - Google Patents

Metodos y kits para detectar secuencias de acidos nucleicos.

Info

Publication number
ES2052607T3
ES2052607T3 ES88100162T ES88100162T ES2052607T3 ES 2052607 T3 ES2052607 T3 ES 2052607T3 ES 88100162 T ES88100162 T ES 88100162T ES 88100162 T ES88100162 T ES 88100162T ES 2052607 T3 ES2052607 T3 ES 2052607T3
Authority
ES
Spain
Prior art keywords
nucleic acid
methods
kits
acid sequences
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88100162T
Other languages
English (en)
Inventor
Mary C Kuhns
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2052607T3 publication Critical patent/ES2052607T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LA INVENCON PROPORCIONA METODOS Y COMPOSICIONES PARA UN ENSAYO CUANTITATIVO RAPIDO DE HIBRIDACION DE ACIDO NUCLEICO PARA DETECTAR UNA SECUENCIA DE ADN O ARN ENUNA MUESTRA BIOLOGICA. EL METODO IMPLICA LA HIBRIDACION EN SOLUCION DE UNA SECUENCIA DE ACIDO NUCLEICO DIANA CON UNA SONDA MARCADA DE MANERA DETECTABLE, SEPARACION DE LA SONDA DE ACIDO NUCLEICO DIANA DE LA SONDA NO HIBRIDIZADA POR CROMATOGRAFIA DE EXCLUSION SOBRE GEL O DE AFINIDAD, Y DETECCION DE LA CANTIDAD DE SONDA MARCADA COMO INDICACION DEL ACIDO NUCLEICO DIANA PRESENTE EN LA MUESTRA BIOLOGICA.
ES88100162T 1987-01-09 1988-01-08 Metodos y kits para detectar secuencias de acidos nucleicos. Expired - Lifetime ES2052607T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US283887A 1987-01-09 1987-01-09

Publications (1)

Publication Number Publication Date
ES2052607T3 true ES2052607T3 (es) 1994-07-16

Family

ID=21702766

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88100162T Expired - Lifetime ES2052607T3 (es) 1987-01-09 1988-01-08 Metodos y kits para detectar secuencias de acidos nucleicos.

Country Status (8)

Country Link
US (1) US5981171A (es)
EP (1) EP0278220B1 (es)
JP (1) JP2690738B2 (es)
AT (1) ATE82773T1 (es)
AU (2) AU619170B2 (es)
CA (1) CA1340840C (es)
DE (1) DE3876108T2 (es)
ES (1) ES2052607T3 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
JP2549530B2 (ja) * 1987-10-01 1996-10-30 新技術事業団 非放射性の核酸標識法及びこれを使用した検出法
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
DE3916871A1 (de) * 1989-05-24 1990-11-29 Boehringer Mannheim Gmbh Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren
JPH0560761A (ja) * 1991-08-30 1993-03-12 Imuno Baion:Kk 重合体の分析方法
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US6203978B1 (en) * 1996-05-31 2001-03-20 Du Vergier Ltd. Capture of single stranded nucleic acids
NL1010012C2 (nl) * 1998-09-04 2000-03-07 Alexander Adrianus Moen Werkwijze voor het detecteren van nucleotidensequenties en/of nucleotide-bindende eiwitten.
US6448422B1 (en) 2000-07-03 2002-09-10 Eli Lilly And Company Chromogenic compound
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
FI115139B (fi) * 2001-01-10 2005-03-15 Valtion Teknillinen Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen
ES2349949T3 (es) * 2001-04-04 2011-01-13 Wako Pure Chemical Industries, Ltd. Electrofóresis con marcador de adn bicatenario.
US6696254B2 (en) * 2001-11-21 2004-02-24 Kimberly-Clark Worldwide, Inc. Detection and identification of enteric bacteria
FI20021325A0 (fi) * 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
US7901883B2 (en) * 2004-12-10 2011-03-08 Genera Biosystems Limited Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS)
US20070042427A1 (en) * 2005-05-03 2007-02-22 Micronics, Inc. Microfluidic laminar flow detection strip
ES2403729T3 (es) * 2007-04-05 2013-05-21 Genera Biosystems Limited Composiciones y métodos de detección
CA2696433A1 (en) * 2007-08-13 2009-03-12 3M Innovative Properties Company Methods for detecting drug-resistant microbes
WO2010048386A1 (en) * 2008-10-24 2010-04-29 Helicos Biosciences Corporation Methods of sample preparation for nucleic acid analysis for nucleic acids available in limited amounts
US8277651B2 (en) 2009-03-13 2012-10-02 Terrasep, Llc Methods and apparatus for centrifugal liquid chromatography
JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
US4407942A (en) * 1981-01-29 1983-10-04 Atomic Energy Of Canada Limited Fluorescent detection of DNA damage
US4399303A (en) * 1981-08-17 1983-08-16 E. I. Du Pont De Nemours And Company Preparation and use of nitroterephthalamic acids
US4894228A (en) * 1982-04-07 1990-01-16 The United States Of America As Represented By The Department Of Health And Human Services Vaccine against hepatitis A virus
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
WO1984003285A1 (en) * 1983-02-22 1984-08-30 Molecular Biosystems Inc Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
IL71699A0 (en) * 1983-05-02 1984-07-31 Enzo Biochem Inc Methods and kits for the analysis and detection of biological or genetic material
CA1231303A (en) * 1983-08-05 1988-01-12 Robert J. Carrico Detection of bacteria by nucleic acid hybridization
FR2552879B1 (fr) * 1983-10-03 1987-08-14 Pasteur Institut Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes
US4599303A (en) * 1983-12-12 1986-07-08 Hri Associates, Inc. Nucleic acid hybridization assay employing probes crosslinkable to target sequences
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU7015287A (en) * 1986-03-19 1987-09-24 Cetus Corporation Detection of nucleic acid sequence using liquid hybridization method
FR2596774B1 (fr) * 1986-04-04 1988-07-22 Pasteur Institut Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants

Also Published As

Publication number Publication date
CA1340840C (en) 1999-12-07
DE3876108D1 (de) 1993-01-07
US5981171A (en) 1999-11-09
ATE82773T1 (de) 1992-12-15
JP2690738B2 (ja) 1997-12-17
AU630836B2 (en) 1992-11-05
EP0278220B1 (en) 1992-11-25
AU619170B2 (en) 1992-01-23
AU1082492A (en) 1992-05-14
DE3876108T2 (de) 1993-04-22
JPS63245698A (ja) 1988-10-12
AU1010688A (en) 1988-07-14
EP0278220A1 (en) 1988-08-17

Similar Documents

Publication Publication Date Title
ES2052607T3 (es) Metodos y kits para detectar secuencias de acidos nucleicos.
Tian et al. Single-strand conformation polymorphism analysis by capillary and microchip electrophoresis: a fast, simple method for detection of common mutations in BRCA1 and BRCA2
ES2083955T3 (es) Determinacion de polinucleotidos con sitios de escision seleccionables.
BR9004881A (pt) Processo para detectar uma sequencia de acido nucleico,processo para determinar quantitativamente a extensao de hibridizacao,ensaio de hibridizacao,estojo,bases,sondas,processo para detectar chlamydia trachomatis e lamina de microtitulacao
ATE66695T1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
BR9509977A (pt) Processo de detecção de ácidos nucleicos com uma composição de sequencia especifica
FI900602A0 (fi) Bestaemning.
DE3474305D1 (en) Probe containing a modified nucleic acid recognizable by specific antibodies and the use to detect and characterize a homologous dna sequence
DK463089A (da) Fremgangsmaade og reagenssaet til detektion af en specifik nucleinsyresekvens i en proeve indeholdende en nucleinsyre
DE68908900D1 (de) Nachweis und bestimmung der menge an rns und dns.
DK163383C (da) Fremgangsmaade og reagenssystem til bestemmelse henholdsvis detektering af en bestemt polynucleotidsekvens i et testmedium indeholdende enkeltstrengede nucleinsyrer
PT887425E (pt) Ensaio de pcr multiplex para detectar infeccao pelo complexo de mycobacterium avium
ATE398186T1 (de) Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren
DE68909445D1 (de) Methode zur bestimmung genetischer sequenzen und testsatz hierfuer.
EP0838527B1 (en) Method for measuring the concentration of polynucleotides
SE8904100D0 (sv) New analysis method
DE69117885D1 (de) Dns-sonden und primer zur erkennung von b. burgdorferi unter verwendung der polymerase-kettenreaktion
DE3567908D1 (en) Photoimmune detection of dna and rna
CA2408734A1 (en) Compositions and methods for genetic analyses

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 278220

Country of ref document: ES