DE3876108D1 - Diagnostischer test unter verwendung von nucleinsaeure-sonden. - Google Patents

Diagnostischer test unter verwendung von nucleinsaeure-sonden.

Info

Publication number
DE3876108D1
DE3876108D1 DE8888100162T DE3876108T DE3876108D1 DE 3876108 D1 DE3876108 D1 DE 3876108D1 DE 8888100162 T DE8888100162 T DE 8888100162T DE 3876108 T DE3876108 T DE 3876108T DE 3876108 D1 DE3876108 D1 DE 3876108D1
Authority
DE
Germany
Prior art keywords
nucleic acid
probe
acid probe
diagnostic test
biological sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8888100162T
Other languages
English (en)
Other versions
DE3876108T2 (de
Inventor
Mary C Kuhns
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of DE3876108D1 publication Critical patent/DE3876108D1/de
Publication of DE3876108T2 publication Critical patent/DE3876108T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE8888100162T 1987-01-09 1988-01-08 Diagnostischer test unter verwendung von nucleinsaeure-sonden. Expired - Fee Related DE3876108T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US283887A 1987-01-09 1987-01-09

Publications (2)

Publication Number Publication Date
DE3876108D1 true DE3876108D1 (de) 1993-01-07
DE3876108T2 DE3876108T2 (de) 1993-04-22

Family

ID=21702766

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8888100162T Expired - Fee Related DE3876108T2 (de) 1987-01-09 1988-01-08 Diagnostischer test unter verwendung von nucleinsaeure-sonden.

Country Status (8)

Country Link
US (1) US5981171A (de)
EP (1) EP0278220B1 (de)
JP (1) JP2690738B2 (de)
AT (1) ATE82773T1 (de)
AU (2) AU619170B2 (de)
CA (1) CA1340840C (de)
DE (1) DE3876108T2 (de)
ES (1) ES2052607T3 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
JP2549530B2 (ja) * 1987-10-01 1996-10-30 新技術事業団 非放射性の核酸標識法及びこれを使用した検出法
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
GB8827160D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
DE3916871A1 (de) * 1989-05-24 1990-11-29 Boehringer Mannheim Gmbh Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren
JPH0560761A (ja) * 1991-08-30 1993-03-12 Imuno Baion:Kk 重合体の分析方法
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US6203978B1 (en) * 1996-05-31 2001-03-20 Du Vergier Ltd. Capture of single stranded nucleic acids
NL1010012C2 (nl) * 1998-09-04 2000-03-07 Alexander Adrianus Moen Werkwijze voor het detecteren van nucleotidensequenties en/of nucleotide-bindende eiwitten.
US6448422B1 (en) 2000-07-03 2002-09-10 Eli Lilly And Company Chromogenic compound
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
FI115139B (fi) * 2001-01-10 2005-03-15 Valtion Teknillinen Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen
JP4214779B2 (ja) * 2001-04-04 2009-01-28 和光純薬工業株式会社 電気泳動法
US6696254B2 (en) * 2001-11-21 2004-02-24 Kimberly-Clark Worldwide, Inc. Detection and identification of enteric bacteria
FI20021325A0 (fi) * 2002-07-05 2002-07-05 Valtion Teknillinen Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi
EP1833985B1 (de) * 2004-12-10 2014-08-27 Genera Biosystems Limited Nachweis von menschlichem papillomavirus unter verwendung von nukleinsäuresonden, mikrokügelchen und eines facs ( fluorescent-activated cell sorter)-geräts
US20070042427A1 (en) * 2005-05-03 2007-02-22 Micronics, Inc. Microfluidic laminar flow detection strip
RU2480732C2 (ru) * 2007-04-05 2013-04-27 Дженера Байосистемз Лимитед Композиции и способы выявления
WO2009032486A1 (en) * 2007-08-13 2009-03-12 3M Innovative Properties Company Methods for detecting drug-resistant microbes
WO2010048386A1 (en) * 2008-10-24 2010-04-29 Helicos Biosciences Corporation Methods of sample preparation for nucleic acid analysis for nucleic acids available in limited amounts
US8293101B2 (en) 2009-03-13 2012-10-23 Terrasep, Llc Methods and apparatus for centrifugal liquid chromatography
WO2011149897A1 (en) 2010-05-25 2011-12-01 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2422956A1 (fr) * 1978-04-13 1979-11-09 Pasteur Institut Procede de detection et de caracterisation d'un acide nucleique ou d'une sequence de celui-ci, et reactif enzymatique pour la mise en oeuvre de ce procede
US4407942A (en) * 1981-01-29 1983-10-04 Atomic Energy Of Canada Limited Fluorescent detection of DNA damage
US4399303A (en) * 1981-08-17 1983-08-16 E. I. Du Pont De Nemours And Company Preparation and use of nitroterephthalamic acids
US4894228A (en) * 1982-04-07 1990-01-16 The United States Of America As Represented By The Department Of Health And Human Services Vaccine against hepatitis A virus
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
AU596068B2 (en) * 1983-02-22 1990-04-26 Syngene, Inc. Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis
ES532041A0 (es) * 1983-05-02 1985-10-01 Enzo Biochem Inc Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico.
CA1231303A (en) * 1983-08-05 1988-01-12 Robert J. Carrico Detection of bacteria by nucleic acid hybridization
FR2552879B1 (fr) * 1983-10-03 1987-08-14 Pasteur Institut Procede et reactifs pour la detection d'une anomalie genetique dans une matiere biologique, notamment dans une preparation de leucocytes
US4599303A (en) * 1983-12-12 1986-07-08 Hri Associates, Inc. Nucleic acid hybridization assay employing probes crosslinkable to target sequences
GB8405437D0 (en) * 1984-03-01 1984-04-04 Amersham Int Plc Detecting polynucleotide sequences
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0238332A2 (de) * 1986-03-19 1987-09-23 Cetus Corporation Flüssiges Hybridisationsverfahren und Satz zum Nachweis der Anwesenheit von Nukleinsäuresequenzen in Proben
FR2596774B1 (fr) * 1986-04-04 1988-07-22 Pasteur Institut Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants

Also Published As

Publication number Publication date
ES2052607T3 (es) 1994-07-16
EP0278220A1 (de) 1988-08-17
JPS63245698A (ja) 1988-10-12
JP2690738B2 (ja) 1997-12-17
DE3876108T2 (de) 1993-04-22
AU630836B2 (en) 1992-11-05
AU1082492A (en) 1992-05-14
AU619170B2 (en) 1992-01-23
AU1010688A (en) 1988-07-14
EP0278220B1 (de) 1992-11-25
CA1340840C (en) 1999-12-07
ATE82773T1 (de) 1992-12-15
US5981171A (en) 1999-11-09

Similar Documents

Publication Publication Date Title
DE3876108D1 (de) Diagnostischer test unter verwendung von nucleinsaeure-sonden.
ES2083955T3 (es) Determinacion de polinucleotidos con sitios de escision seleccionables.
HUP9900459A2 (hu) Eljárások és készítmények ligandumpár kötődésének nem fluoreszcens jelölőanyag alkalmazásával történő kimutatására
ATE66695T1 (de) Verfahren zur erhoehung der empfindlichkeit von nukleinsaeure-hybridisierungstesten.
ES8507177A1 (es) Un metodo para detectar bacterias en una muestra a ensayar
DE3578349D1 (de) Hybridisierungstest fuer nukleinsaeure mittels anwendung von nachweisbaren anti-hybridantikoerpern.
ATE198912T1 (de) Homogenes testsystem
DE3474305D1 (en) Probe containing a modified nucleic acid recognizable by specific antibodies and the use to detect and characterize a homologous dna sequence
DE3785865D1 (de) Schnellverfahren zum nachweis von nukleinsaeure-sequenzen in einer probe mittels probemarkierung.
DE68908054D1 (de) Verstaerkung und nachweis von nukleinsaeuresequenzen.
DE69938623D1 (de) Microarrays und ihre verwendungen
DE59814239D1 (de) Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
ATE78301T1 (de) Molekulare genetische sonde und verfahren zu ihrer herstellung, testmethode und satz in denen diese molekulare genetische sonde gebraucht wird.
EP3036341B1 (de) Nachweis von nukleinsäureamplifikation in einem porösen substrat
US5618671A (en) Method and system for molecular-biological diagnostics
US6423490B1 (en) Method for measuring the concentration of polynucleotides
EP0366813A4 (en) Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms
Nagahata et al. Application of the fluorometric dna quantitative assay using 4’6-diamidine-2-phenylindole for the evaluation of lymphocyte blastogenic response
DE3567908D1 (en) Photoimmune detection of dna and rna
JPS6439553A (en) Hybridization assay method for nucleic acid

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee