JPS6439553A - Hybridization assay method for nucleic acid - Google Patents

Hybridization assay method for nucleic acid

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Publication number
JPS6439553A
JPS6439553A JP19531287A JP19531287A JPS6439553A JP S6439553 A JPS6439553 A JP S6439553A JP 19531287 A JP19531287 A JP 19531287A JP 19531287 A JP19531287 A JP 19531287A JP S6439553 A JPS6439553 A JP S6439553A
Authority
JP
Japan
Prior art keywords
nucleic acid
probe
executed
determination
base sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP19531287A
Other languages
Japanese (ja)
Inventor
Akio Takahashi
Shigetami Mitoma
Kazumi Terashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tosoh Corp
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Priority to JP19531287A priority Critical patent/JPS6439553A/en
Publication of JPS6439553A publication Critical patent/JPS6439553A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To make simple and rapid detection and/or determination of nucleic acid having specific base sequence by using liquid chromatography. CONSTITUTION:The detection and/or determination of the nucleic acid having the specific base sequence is executed by using the liquid chromatography. Materials which are used in general clinical inspections, for example, blood cells, excretas, tissue homogenate, etc., or cultivated microorganism, mammal cells, etc., are used as the material for assay. The probe nucleic acid has the length of preferably about >=8 bases and is the fragment derived from natural nucleic acids or synthetic nucleic acid in terms of specificness and stability. A fluorescent material, chemical or bioluminescent material, enzyme, radioactive isotope,etc., are used for labeling the probe. The reaction soln. of the hybridization assay is sent through an injection device 1 and a liquid feed pump 3 to a column 4 where the sepn. of the complex hybridized with the nucleic probe to the target nucleic acid and the unhybridized probe in a free state is executed by rinsing. Both the probes are thereafter detected by detectors 5, 6 and are recorded 7.
JP19531287A 1987-08-06 1987-08-06 Hybridization assay method for nucleic acid Pending JPS6439553A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP19531287A JPS6439553A (en) 1987-08-06 1987-08-06 Hybridization assay method for nucleic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP19531287A JPS6439553A (en) 1987-08-06 1987-08-06 Hybridization assay method for nucleic acid

Publications (1)

Publication Number Publication Date
JPS6439553A true JPS6439553A (en) 1989-02-09

Family

ID=16339061

Family Applications (1)

Application Number Title Priority Date Filing Date
JP19531287A Pending JPS6439553A (en) 1987-08-06 1987-08-06 Hybridization assay method for nucleic acid

Country Status (1)

Country Link
JP (1) JPS6439553A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2065708A1 (en) 2007-11-28 2009-06-03 Fujifilm Corporation Method for measuring high-density lipoprotein cholesterol

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2065708A1 (en) 2007-11-28 2009-06-03 Fujifilm Corporation Method for measuring high-density lipoprotein cholesterol

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