JPS6439553A - Hybridization assay method for nucleic acid - Google Patents
Hybridization assay method for nucleic acidInfo
- Publication number
- JPS6439553A JPS6439553A JP19531287A JP19531287A JPS6439553A JP S6439553 A JPS6439553 A JP S6439553A JP 19531287 A JP19531287 A JP 19531287A JP 19531287 A JP19531287 A JP 19531287A JP S6439553 A JPS6439553 A JP S6439553A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- probe
- executed
- determination
- base sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To make simple and rapid detection and/or determination of nucleic acid having specific base sequence by using liquid chromatography. CONSTITUTION:The detection and/or determination of the nucleic acid having the specific base sequence is executed by using the liquid chromatography. Materials which are used in general clinical inspections, for example, blood cells, excretas, tissue homogenate, etc., or cultivated microorganism, mammal cells, etc., are used as the material for assay. The probe nucleic acid has the length of preferably about >=8 bases and is the fragment derived from natural nucleic acids or synthetic nucleic acid in terms of specificness and stability. A fluorescent material, chemical or bioluminescent material, enzyme, radioactive isotope,etc., are used for labeling the probe. The reaction soln. of the hybridization assay is sent through an injection device 1 and a liquid feed pump 3 to a column 4 where the sepn. of the complex hybridized with the nucleic probe to the target nucleic acid and the unhybridized probe in a free state is executed by rinsing. Both the probes are thereafter detected by detectors 5, 6 and are recorded 7.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19531287A JPS6439553A (en) | 1987-08-06 | 1987-08-06 | Hybridization assay method for nucleic acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19531287A JPS6439553A (en) | 1987-08-06 | 1987-08-06 | Hybridization assay method for nucleic acid |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6439553A true JPS6439553A (en) | 1989-02-09 |
Family
ID=16339061
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP19531287A Pending JPS6439553A (en) | 1987-08-06 | 1987-08-06 | Hybridization assay method for nucleic acid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6439553A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2065708A1 (en) | 2007-11-28 | 2009-06-03 | Fujifilm Corporation | Method for measuring high-density lipoprotein cholesterol |
-
1987
- 1987-08-06 JP JP19531287A patent/JPS6439553A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2065708A1 (en) | 2007-11-28 | 2009-06-03 | Fujifilm Corporation | Method for measuring high-density lipoprotein cholesterol |
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