ES8507177A1 - Un metodo para detectar bacterias en una muestra a ensayar - Google Patents

Un metodo para detectar bacterias en una muestra a ensayar

Info

Publication number
ES8507177A1
ES8507177A1 ES534864A ES534864A ES8507177A1 ES 8507177 A1 ES8507177 A1 ES 8507177A1 ES 534864 A ES534864 A ES 534864A ES 534864 A ES534864 A ES 534864A ES 8507177 A1 ES8507177 A1 ES 8507177A1
Authority
ES
Spain
Prior art keywords
bacteria
nucleic acid
base sequence
detecting
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534864A
Other languages
English (en)
Other versions
ES534864A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES534864A0 publication Critical patent/ES534864A0/es
Publication of ES8507177A1 publication Critical patent/ES8507177A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO PARA DETECTAR BACTERIAS EN UNA MUESTRA A ENSAYAR.COMPRENDE: A) SOMETER LA MUESTRA A ENSAYAR A CONDICIONES QUE LIBERAN Y DESNATURALIZAN LOS ACIDOS NUCLEICOS DE LAS BACTERIAS PRESENTES EN LA MUESTRA; B) INMOVILIZAR LOS ACIDOS NUCLEICOS MONOCATENARIOS RESULTANTES DEL TRATAMIENTO DE LA MUESTRA A ENSAYAR SOBRE UN SOPORTE SOLIDO; C) PONER EN CONTACTO EL SOPORTE SOLIDO CON UNA SONDA POLINUCLEOTIDICA MARCADA QUE CONTIENE UNA SECUENCIA DE BASES CON UN FRAGMENTO DE DIEZ BASES DE UNA DE LAS CADENAS DE UN GEN TUF Y FUS; D) SEPARAR LA SONDA MARCADA NO HIBRIDADA DEL SOPORTE SOLIDO Y E) DETERMINAR LA SONDA MARCADA HIBRIDADA ASOCIADA AL SOPORTE MEDIANTE MEDIDA DEL MARCADOR CONTENIDO EN LA SONDA MARCADA.
ES534864A 1983-08-05 1984-08-03 Un metodo para detectar bacterias en una muestra a ensayar Expired ES8507177A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US52052483A 1983-08-05 1983-08-05

Publications (2)

Publication Number Publication Date
ES534864A0 ES534864A0 (es) 1985-08-16
ES8507177A1 true ES8507177A1 (es) 1985-08-16

Family

ID=24072977

Family Applications (1)

Application Number Title Priority Date Filing Date
ES534864A Expired ES8507177A1 (es) 1983-08-05 1984-08-03 Un metodo para detectar bacterias en una muestra a ensayar

Country Status (7)

Country Link
EP (1) EP0133671B1 (es)
JP (1) JPS60100056A (es)
AU (1) AU3138784A (es)
CA (1) CA1231303A (es)
DE (1) DE3484832D1 (es)
ES (1) ES8507177A1 (es)
IL (1) IL72499A (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723597A (en) * 1983-01-10 1998-03-03 Gen-Probe Incorporated Ribosomal nucleic acid probes for detecting organisms or groups of organisms
US5641631A (en) * 1983-01-10 1997-06-24 Gen-Probe Incorporated Method for detecting, identifying, and quantitating organisms and viruses
US5593832A (en) * 1983-02-28 1997-01-14 Lifecodes Corporation Method for forensic analysis
EP0144914A3 (en) * 1983-12-12 1986-08-13 Miles Inc. Hybridization assay employing labeled pairs of hybrid binding reagents
EP0146039A3 (en) * 1983-12-12 1986-08-27 Miles Inc. Hybridization assay with immobilization of hybrids by antihybrid binding
EP0144913A3 (en) * 1983-12-12 1986-08-20 Miles Inc. Hybridization assay employing labeled probe and anti-hybrid
CA1253777A (en) * 1984-06-01 1989-05-09 Robert J. Carrico Nucleic acid hybridization assay employing immobilized rna probes
US5955261A (en) * 1984-09-04 1999-09-21 Gen-Probe Incorporated Method for detecting the presence of group-specific viral mRNA in a sample
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
JPS6283888A (ja) * 1985-09-30 1987-04-17 Sumitomo Chem Co Ltd ポリペプチド鎖伸長因子−２ｃＤＮＡとその利用法
EP0253894A1 (en) * 1985-12-27 1988-01-27 Toray Industries, Inc. Dna probe and method of preparing the same
GB8608269D0 (en) * 1986-04-04 1986-05-08 Brown J J Ice-maker
GB8608629D0 (en) * 1986-04-09 1986-05-14 Biotechnica Ltd Labelling
EP0277237A4 (en) * 1986-07-10 1990-02-22 Toray Industries METHOD AND PROBE FOR SCREENING FOR BACTERIA IN SAMPLES.
US7087742B1 (en) 1986-11-24 2006-08-08 Gen-Probe Incorporated Oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US5541308A (en) * 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US6150517A (en) 1986-11-24 2000-11-21 Gen-Probe Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US5994059A (en) * 1986-11-24 1999-11-30 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Streptomyces enterococci
US7138516B1 (en) 1986-11-24 2006-11-21 Gen-Probe Incorporated Oligonucleotide probes for the detection and/or quantitation of non-viral organisms
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
US5089386A (en) * 1987-09-11 1992-02-18 Gene-Trak Systems Test for listeria
ATE133454T1 (de) * 1987-12-01 1996-02-15 Amoco Corp Nachweis von salmonella
AU623353B2 (en) * 1987-12-10 1992-05-14 Board Of Regents, The University Of Texas System Methods and compositions for the production of haemophilus influenzae type b major outer membrane protein antigens
AU627712B2 (en) * 1988-01-11 1992-09-03 Becton Dickinson & Company Oligonucleotide probes for detection of periodontal pathogens
US5212059A (en) * 1988-01-11 1993-05-18 Microprobe Corporation Oligonucleotide probes for the detection of periodontal pathogens
US7172863B1 (en) 1988-12-09 2007-02-06 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Neisseria gonorrhoeae
US5472843A (en) * 1991-04-25 1995-12-05 Gen-Probe Incorporated Nucleic acid probes to Haemophilus influenzae
US5232831A (en) * 1991-06-28 1993-08-03 Gen-Probe Incorporated Nucleic acid probes to streptococcus pyogenes
US6028187A (en) * 1991-08-01 2000-02-22 Gen-Probe Incorporated Nucleic acid probes to Listeria monocytogenes
US5292874A (en) * 1991-09-04 1994-03-08 Gen-Probe Incorporated Nucleic acid probes to Staphylococcus aureus
US5738987A (en) * 1991-09-04 1998-04-14 Gen-Probe Incorporated 16S ribosomal nucleic acid probes to streptococcus pneumoniae
US5558990A (en) * 1991-12-18 1996-09-24 Gen-Probe Incorporated Nucleic acid probes to blastomyces dermatitidis and paracoccidioides brasiliensis
US5284747A (en) * 1991-12-18 1994-02-08 Gen-Probe Incorporated Nucleic acid probes to Coccidioides immitis
US20020055101A1 (en) 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
JP3093116B2 (ja) * 1994-09-30 2000-10-03 株式会社豊田中央研究所 核酸検出方法
US5622827A (en) * 1995-02-07 1997-04-22 Gen-Probe Incorporated Amplification primers and nucleic acid probes targeted to coccidioides immitis nucleic acid
FR2755145B1 (fr) 1996-10-28 1999-01-15 Gervais Danone Co Procede de mise en evidence de contaminants microbiologiques vivants dans un echantillon de produit a usage alimentaire
US20030049636A1 (en) 1999-05-03 2003-03-13 Bergeron Michel G. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US6468743B1 (en) * 1998-05-18 2002-10-22 Conagra Grocery Products Company PCR techniques for detecting microbial contaminants in foodstuffs
BR0014370A (pt) 1999-09-28 2002-11-05 Infectio Diagnostic Inc Genes altamente conservados e seu uso para gerar sondas de ácido nucléico e preparadores de amplificação especìficos em espécie, especìfico em gênero, especìficos em famìlia, especìficos em grupo e universais para rapidamente detectar e identificar microorganismos algais, amebianos, bacterianos, fúngicos e parasitas de espécimes clìnicos para diagnose
US20220396824A1 (en) * 2019-09-24 2022-12-15 Genepath Diagnostics Inc. Composition and method for improving detection of biomolecules in biofluid samples

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4717653A (en) * 1981-09-25 1988-01-05 Webster John A Jr Method for identifying and characterizing organisms
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser

Also Published As

Publication number Publication date
EP0133671A2 (en) 1985-03-06
IL72499A (en) 1989-02-28
EP0133671B1 (en) 1991-07-24
AU3138784A (en) 1985-02-07
ES534864A0 (es) 1985-08-16
JPS60100056A (ja) 1985-06-03
DE3484832D1 (de) 1991-08-29
IL72499A0 (en) 1984-11-30
EP0133671A3 (en) 1987-12-23
CA1231303A (en) 1988-01-12

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000503