KR870009030A - 미생물의 신속한 dna시험 - Google Patents
미생물의 신속한 dna시험 Download PDFInfo
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- KR870009030A KR870009030A KR870001997A KR870001997A KR870009030A KR 870009030 A KR870009030 A KR 870009030A KR 870001997 A KR870001997 A KR 870001997A KR 870001997 A KR870001997 A KR 870001997A KR 870009030 A KR870009030 A KR 870009030A
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- microorganism
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- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (10)
- (1) 시료를 고정시키고,(2) 헥산을 변성시키고(c) 시료를 혼성화 조건하에서 분석될 미생물의 분할되지 않고, 표지되고, 변성시킨 완전한 게놈 DNA프로브와 접촉시키고,(d) 표지를 검출함으로써 혼성된 핵산을 분석하는 것으로 되는, 특별한 미생물의 존재에 대한 생물학적 핵산―함유시료를 분석하는 방법.
- 제 1항에 있어서, 미생물이 박테리아, 진군, 비루수 및 원생동물로 되는 군중에서 선택됨을 특징으로 하는 방법.
- 제 1항 또는 제 2항 중 어느 하나의 항에 있어서, 미생물이 요로감염의 원인이 되는 것을 특징으로 하는 방법.
- 제 1항 내지 3항 중 어느 하나의 항에 있어서, 미생물이 대장균, 변형균속, 협막간균속, 포도상구군, 연쇄상구균속, 슈도모나스속 및 유산간균속으로 되는 군중에서 선택됨을 특징으로 하는 방법.
- 제 1항 내지 4항 중 어느 하나의 항에 있어서, 라벨링을 완전한 생세포, 세포용해질 중에서 또는 추출시킨 핵산으로 행함을 특징으로 하는 방법.
- 제 1항 내지 5항 중 어느 하나의 항에 있어서, 표지가 단백질 결합 리간드, 합텐, 항원, 형광 화합물 염료, 방사성 부분 및 효소로 되는 군중에서 선택됨을 특징으로 하는 방법.
- 제 6항에 있어서, 단백질결합 리간드가 비오틴 함유부분인 것을 특징으로 하는 방법.
- 제 1항 내지 7항 중 어느 하나의 항에 있어서, 표지의 검출을 화학적 루미네센스 또는 효소반응에 의해 행함을 특징으로 하는 방법.
- 제 1항 내지 8항 중 어느 하나의 항에 있어서, 고정화가 필터, 고체시트 및 비드로 되는 군중에서 선택 지지대를 사용하여 화학적 반응 또는 물리적 흡착에 의해 행해짐을 특징으로 하는 방법.
- 제 1항에 있어서, 상기 라벨링을 DNA결합 리간드와 프로브중의핵산을 광화학적으로 반응시켜서 행함을 특징으로 하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US83678786A | 1986-03-06 | 1986-03-06 | |
US836787 | 1986-03-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
KR870009030A true KR870009030A (ko) | 1987-10-22 |
Family
ID=25272733
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR870001997A KR870009030A (ko) | 1986-03-06 | 1987-03-06 | 미생물의 신속한 dna시험 |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0235727A3 (ko) |
JP (1) | JPS62266000A (ko) |
KR (1) | KR870009030A (ko) |
AU (1) | AU6972287A (ko) |
DK (1) | DK114387A (ko) |
FI (1) | FI870946A (ko) |
IL (1) | IL81741A0 (ko) |
NO (1) | NO870614L (ko) |
ZA (1) | ZA871581B (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100691806B1 (ko) * | 2005-08-04 | 2007-03-12 | 삼성전자주식회사 | 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치 |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4876186A (en) * | 1985-11-05 | 1989-10-24 | Battelle Development Corporation | Detection and differentiation of coxiella burnetii in biological fluids |
NO870613L (no) * | 1986-03-05 | 1987-09-07 | Molecular Diagnostics Inc | Deteksjon av mikroorganismer i en prve inneholdende nukleinsyre. |
AU601021B2 (en) * | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
JP2589729B2 (ja) * | 1988-01-30 | 1997-03-12 | 極東製薬工業株式会社 | 染色体dnaを用いた細菌の同定方法および該同定用キット |
IL87668A0 (en) * | 1988-09-04 | 1989-02-28 | Yeda Res & Dev | Assay for amoebas |
IE64040B1 (en) * | 1989-06-15 | 1995-06-28 | Akzo Nv | Method for determining nucleic acid |
FR2674632B1 (fr) * | 1991-03-29 | 1994-07-22 | Bertin & Cie | Procede et dispositif de detection rapide de micro-organismes indesirables dans un produit du domaine agro-alimentaire ou medico-veterinaire. |
ES2097701B1 (es) * | 1995-02-09 | 1997-11-16 | Consejo Superior Investigacion | Procedimiento para obtener sondas especificas de estirpe o de especie. |
JP2009034052A (ja) * | 2007-08-02 | 2009-02-19 | Canon Inc | ハイブリダイゼーション方法および装置 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4446237A (en) * | 1981-03-27 | 1984-05-01 | Life Technologies, Inc. | Method for detection of a suspect viral deoxyribonucleic acid in an acellular biological fluid |
EP0062286A1 (en) * | 1981-03-31 | 1982-10-13 | Albert Einstein College Of Medicine Of Yeshiva University | Diagnostic test for hepatitis B virus |
EP0119209A1 (en) * | 1982-09-20 | 1984-09-26 | President And Fellows Of Harvard College | Identification of microorganisms |
US4737454A (en) * | 1983-07-14 | 1988-04-12 | Molecular Diagnostics, Inc. | Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays |
IL71715A0 (en) * | 1983-08-05 | 1984-09-30 | Miles Lab | Method,gene probes and test kit for detecting bacteria by nucleic acid hybridization |
-
1987
- 1987-02-17 NO NO870614A patent/NO870614L/no unknown
- 1987-02-24 EP EP87102580A patent/EP0235727A3/en not_active Withdrawn
- 1987-03-03 IL IL81741A patent/IL81741A0/xx unknown
- 1987-03-04 FI FI870946A patent/FI870946A/fi not_active Application Discontinuation
- 1987-03-05 DK DK114387A patent/DK114387A/da not_active Application Discontinuation
- 1987-03-05 AU AU69722/87A patent/AU6972287A/en not_active Abandoned
- 1987-03-05 ZA ZA871581A patent/ZA871581B/xx unknown
- 1987-03-06 KR KR870001997A patent/KR870009030A/ko not_active IP Right Cessation
- 1987-03-06 JP JP62051844A patent/JPS62266000A/ja active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100691806B1 (ko) * | 2005-08-04 | 2007-03-12 | 삼성전자주식회사 | 비드 및 나노포어를 이용한 핵산 검출방법 및 검출장치 |
Also Published As
Publication number | Publication date |
---|---|
IL81741A0 (en) | 1987-10-20 |
FI870946A (fi) | 1987-09-07 |
DK114387D0 (da) | 1987-03-05 |
JPS62266000A (ja) | 1987-11-18 |
EP0235727A2 (en) | 1987-09-09 |
ZA871581B (en) | 1987-12-30 |
DK114387A (da) | 1987-09-07 |
FI870946A0 (fi) | 1987-03-04 |
NO870614L (no) | 1987-09-07 |
EP0235727A3 (en) | 1990-02-14 |
AU6972287A (en) | 1987-09-10 |
NO870614D0 (no) | 1987-02-17 |
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SUBM | Surrender of laid-open application requested |