ES8507178A1 - Un metodo para detectar bacterias en una muestra a ensayar - Google Patents

Un metodo para detectar bacterias en una muestra a ensayar

Info

Publication number
ES8507178A1
ES8507178A1 ES534865A ES534865A ES8507178A1 ES 8507178 A1 ES8507178 A1 ES 8507178A1 ES 534865 A ES534865 A ES 534865A ES 534865 A ES534865 A ES 534865A ES 8507178 A1 ES8507178 A1 ES 8507178A1
Authority
ES
Spain
Prior art keywords
detecting
bacteria
nucleic acid
labeled probes
acid hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534865A
Other languages
English (en)
Other versions
ES534865A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES8507178A1 publication Critical patent/ES8507178A1/es
Publication of ES534865A0 publication Critical patent/ES534865A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

METODO PARA DETECTAR BACTERIAS EN UNA MUESTRA A ENSAYAR.COMPRENDE: A) SOMETER LA MUESTRA A ENSAYAR A CONDICIONES QUE LIBERAN Y DESNATURALIZAN LOS ACIDOS NUCLEICOS DE LAS BACTERIAS PRESENTES EN LA MUESTRA, B) PONER EN CONTACTO LOS ACIDOS NUCLEICOS MONOCATENARIOS RESULTANTES CON UNA SONDA POLINUCLEOTIDICA, EN CONDICIONES FAVORABLES A LA HIBRIDACION ENTRE LAS SECUENCIAS DE BASES DE LA SONDA Y BACTERIANAS Y C) DETERMINAR LA PRESENCIA DE LA SONDA HIBRIDADA. LA SONDA CONSISTE EN UNA SECUENCIA DE BASES HOMOLOGA A UNA SECUENCIA DE BASES DE LOS ACIDOS NUCLEICOS DE LA TOTALIDAD DE LAS BACTERIAS CUYA PRESENCIA EN LA MUESTRA A ENSAYAR SE SOSPECHA. LA SECUENCIA DE BASES HOMOLOGA TIENE UN FRAGMENTO DE UNA DE LAS CADENAS DE UN GEN QUE CODIFICA EN LA SINTESIS DE UN ACIDO NUCLEICO O PROTEINA. EL GEN CODIFICA PARA LA SINTESIS DE UN ACIDO NUCLEICO O PROTEINA SELECCIONADOS ENTRE RNAS DE TRANSFERENCIA RNAS RIBOSOMICOS PROTEINAS RIBOSOMIAS Y OTROS.
ES534865A 1983-08-05 1984-08-03 Un metodo para detectar bacterias en una muestra a ensayar Granted ES534865A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US52052583A 1983-08-05 1983-08-05

Publications (2)

Publication Number Publication Date
ES8507178A1 true ES8507178A1 (es) 1985-08-16
ES534865A0 ES534865A0 (es) 1985-08-16

Family

ID=24072980

Family Applications (1)

Application Number Title Priority Date Filing Date
ES534865A Granted ES534865A0 (es) 1983-08-05 1984-08-03 Un metodo para detectar bacterias en una muestra a ensayar

Country Status (5)

Country Link
EP (1) EP0133288A2 (es)
JP (1) JPS60110299A (es)
AU (1) AU3138884A (es)
ES (1) ES534865A0 (es)
IL (2) IL71715A0 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593832A (en) * 1983-02-28 1997-01-14 Lifecodes Corporation Method for forensic analysis
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4766064A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay employing polyether and diagnostic kit
IL75648A0 (en) * 1984-07-05 1985-10-31 Gen Probe Inc Accelerated nucleic acid reassociation method
FR2568588B1 (fr) * 1984-08-02 1987-02-13 Inst Nat Sante Rech Med Sonde et procede pour la detection de micro-organismes determines, notamment des legionella dans les milieux les contenant
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
EP0239613B1 (en) * 1985-09-26 1990-08-16 The University of Southern Mississippi Piezoelectric device for detection of polynucleotide hybridization
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
ATE85088T1 (de) * 1986-01-07 1993-02-15 Gen Probe Inc Verfahren der beschleunigten wiederassoziation der nukleinsaeure.
NO870614L (no) * 1986-03-06 1987-09-07 Molecular Diagnostics Inc Rask dna-pŸvisning av mikrober.
US20020055101A1 (en) 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US5994066A (en) * 1995-09-11 1999-11-30 Infectio Diagnostic, Inc. Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US20030049636A1 (en) 1999-05-03 2003-03-13 Bergeron Michel G. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
WO1999037804A2 (en) * 1998-01-23 1999-07-29 Akzo Nobel N.V. EF-Tu mRNA AS A MARKER FOR VIABILITY OF BACTERIA
US6664045B1 (en) 1998-06-18 2003-12-16 Boston Probes, Inc. PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms
EP1246935B1 (en) 1999-09-28 2013-08-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
WO2006012727A1 (en) * 2004-08-02 2006-02-09 Infectio Recherche Inc. Capture probe design for efficient hybridisation
WO2007012131A1 (en) * 2005-07-27 2007-02-01 Diagnostic Array Systems Pty Ltd Method for the detection and/or identification of a microorganism
AU2006274507B2 (en) * 2005-07-27 2013-08-29 Diagnostic Array Systems Pty Ltd Method for the detection and/or identification of a microorganism
JP5593037B2 (ja) * 2009-03-12 2014-09-17 東洋水産株式会社 細菌測定用プライマーおよび細菌測定方法
AU2016262545B2 (en) * 2015-05-12 2022-06-16 Gen-Probe Incorporated Method of pooling blood samples
CN114164100A (zh) * 2021-12-31 2022-03-11 上海山恒生态科技股份有限公司 一种环保好氧菌培养用杂交仪

Also Published As

Publication number Publication date
IL71715A0 (en) 1984-09-30
AU3138884A (en) 1985-02-07
IL72501A0 (en) 1984-11-30
JPS60110299A (ja) 1985-06-15
EP0133288A2 (en) 1985-02-20
ES534865A0 (es) 1985-08-16

Similar Documents

Publication Publication Date Title
ES8507178A1 (es) Un metodo para detectar bacterias en una muestra a ensayar
ES8507177A1 (es) Un metodo para detectar bacterias en una muestra a ensayar
McDaniels et al. Confirmational identification of Escherichia coli, a comparison of genotypic and phenotypic assays for glutamate decarboxylase and beta-D-glucuronidase
EP0309230A3 (en) Homogeneous protection assay
EP0188450A4 (en) METHOD FOR DETECTING PATHOGENS OR GENETIC UNITS UNKNOWN IN VITRO AND DETERMINING.
Rasmussen et al. Combined polymerase chain reaction-hybridization microplate assay used to detect bovine leukemia virus and Salmonella
DE69807293T2 (de) Multiplex-PCR zum Nachweis von Infektionen verursacht durch Mycobacterium Avium Komplex
EP0122028A1 (en) Colorimetric assay for enzymes, diagnostic article therefor and a method for forming such article
Zaouri et al. DNAzyme-based biosensor as a rapid and accurate verification tool to complement simultaneous enzyme-based media for E. coli detection
US6268143B1 (en) Automated high throughput E. coli o157:H7 PCR detection system and uses thereof
Bossuyt et al. Impedance measurements to detect post-pasteurization contamination of pasteurized milk
EP0851941B1 (en) Control for pcr
KR870009030A (ko) 미생물의 신속한 dna시험
Doğan et al. Evaluation of two commercial kits and arbitrarily primed PCR for identification and differentiation of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus
JPH01252299A (ja) 飲料水、浴用水及び排水、食品及び飲物中の腸内バクテリアの確認方法
Ross et al. DNA polymerase III-dependent repair synthesis in response to bleomycin in toluene-treated Escherichia coli
Kasprowicz et al. Diagnostics: Routine Identification on Standard and Chromogenic Media, and Advanced Automated Methods
WO1991019003A1 (en) Detection process
Cundell Rapid counting methods for coliform bacteria
Sharpe Developments in rapid methods for detection of agents of foodborne disease
EP0929695A1 (en) Detection of psychrotrophic bacillus
Varghese et al. Nucleic Acid-Based Molecular Techniques in Microbial Food Safety
Ozkizilcik et al. Comparison of real-time PCR and conventional cultural methods to detect Escherichia coli O157: H7 in spices in Turkey
Geldreich et al. Searching for rapid methods in environmental bacteriology
Russell et al. Miniaturized biochemical procedures for identification of bacteria