DE60128908D1 - Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA - Google Patents

Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA

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Publication number
DE60128908D1
DE60128908D1 DE60128908T DE60128908T DE60128908D1 DE 60128908 D1 DE60128908 D1 DE 60128908D1 DE 60128908 T DE60128908 T DE 60128908T DE 60128908 T DE60128908 T DE 60128908T DE 60128908 D1 DE60128908 D1 DE 60128908D1
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DE
Germany
Prior art keywords
nucleic acid
situ
detection
hybridization
localization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60128908T
Other languages
English (en)
Other versions
DE60128908T2 (de
Inventor
Daryn Kenny
Lu Ping Shen
Vincent P Antao
Audrey N Player
Wei Cao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics Inc
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Bayer Corp
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Publication date
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Publication of DE60128908D1 publication Critical patent/DE60128908D1/de
Application granted granted Critical
Publication of DE60128908T2 publication Critical patent/DE60128908T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60128908T 2000-06-02 2001-06-01 Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA Expired - Lifetime DE60128908T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20913900P 2000-06-02 2000-06-02
US209139P 2000-06-02
PCT/US2001/017595 WO2001094632A2 (en) 2000-06-02 2001-06-01 Highly sensitive gene detection and localization using in situ branched-dna hybridization

Publications (2)

Publication Number Publication Date
DE60128908D1 true DE60128908D1 (de) 2007-07-26
DE60128908T2 DE60128908T2 (de) 2008-02-28

Family

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Family Applications (1)

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DE60128908T Expired - Lifetime DE60128908T2 (de) 2000-06-02 2001-06-01 Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA

Country Status (7)

Country Link
US (1) US7033758B2 (de)
EP (1) EP1287165B1 (de)
JP (1) JP2003535600A (de)
AT (1) ATE364722T1 (de)
DE (1) DE60128908T2 (de)
ES (1) ES2287134T3 (de)
WO (1) WO2001094632A2 (de)

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Also Published As

Publication number Publication date
US7033758B2 (en) 2006-04-25
DE60128908T2 (de) 2008-02-28
ES2287134T3 (es) 2007-12-16
WO2001094632A3 (en) 2002-05-30
ATE364722T1 (de) 2007-07-15
US20020172950A1 (en) 2002-11-21
WO2001094632A2 (en) 2001-12-13
EP1287165B1 (de) 2007-06-13
JP2003535600A (ja) 2003-12-02
EP1287165A2 (de) 2003-03-05

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: SIEMENS HEALTHCARE DIAGNOSTICS INC. (N.D.GES.D, US

8328 Change in the person/name/address of the agent

Representative=s name: MAIER, D., DIPL.-ING. UNIV., PAT.-ASS., 81739 MUEN