ATE307903T1 - Immunologischer nachweis von rna:dna hybriden auf mikroarrays - Google Patents
Immunologischer nachweis von rna:dna hybriden auf mikroarraysInfo
- Publication number
- ATE307903T1 ATE307903T1 AT00980379T AT00980379T ATE307903T1 AT E307903 T1 ATE307903 T1 AT E307903T1 AT 00980379 T AT00980379 T AT 00980379T AT 00980379 T AT00980379 T AT 00980379T AT E307903 T1 ATE307903 T1 AT E307903T1
- Authority
- AT
- Austria
- Prior art keywords
- biological molecules
- rna
- microarrays
- hybrid
- kit
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/440,419 US6277579B1 (en) | 1998-02-06 | 1999-11-15 | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
US09/707,178 US6686151B1 (en) | 1998-02-06 | 2000-11-06 | Immunological detection of RNA:DNA hybrids on microarrays |
PCT/US2000/031277 WO2001036681A2 (en) | 1999-11-15 | 2000-11-14 | Immunological detection of rna:dna hybrids on microarrays |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE307903T1 true ATE307903T1 (de) | 2005-11-15 |
Family
ID=27032416
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT00980379T ATE307903T1 (de) | 1999-11-15 | 2000-11-14 | Immunologischer nachweis von rna:dna hybriden auf mikroarrays |
Country Status (8)
Country | Link |
---|---|
US (1) | US6686151B1 (de) |
EP (1) | EP1230396B1 (de) |
AT (1) | ATE307903T1 (de) |
AU (1) | AU784821B2 (de) |
BR (1) | BRPI0015582B1 (de) |
CA (1) | CA2391558A1 (de) |
DE (1) | DE60023539T2 (de) |
WO (1) | WO2001036681A2 (de) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2246546T3 (es) * | 1997-12-12 | 2006-02-16 | Digene Corporation | Valoracion de enfermedades relacionadas con el virus de papiloma humano. |
US7399589B2 (en) | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
GB0105790D0 (en) * | 2001-03-08 | 2001-04-25 | Expresson Biosystems Ltd | Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes |
US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
US20040161741A1 (en) | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
US9777312B2 (en) | 2001-06-30 | 2017-10-03 | Enzo Life Sciences, Inc. | Dual polarity analysis of nucleic acids |
US20050095646A1 (en) * | 2001-11-19 | 2005-05-05 | Sherman Michael I. | Method of using a non-antibody protein to detect and measure an analyte |
US20040067492A1 (en) * | 2002-10-04 | 2004-04-08 | Allan Peng | Reverse transcription on microarrays |
US7560232B2 (en) * | 2002-12-19 | 2009-07-14 | Promega Corporation | Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein |
JP3920223B2 (ja) * | 2003-01-07 | 2007-05-30 | 日本碍子株式会社 | 反応性チップと、このチップを用いた標的物質の結合検出方法 |
WO2005003318A2 (en) * | 2003-07-02 | 2005-01-13 | Perkinelmer Las, Inc. | Assay and process for labeling and detection of micro rna and small interfering rna sequences |
ATE289685T1 (de) * | 2003-08-25 | 2005-03-15 | Mtm Lab Ag | Verfahren zum nachweis von karzinomen in solubilisierten zervikalen körperproben |
US7964344B2 (en) * | 2003-09-17 | 2011-06-21 | Canon Kabushiki Kaisha | Stable hybrid |
US7901880B2 (en) | 2003-10-21 | 2011-03-08 | Orion Genomics Llc | Differential enzymatic fragmentation |
WO2006023312A2 (en) * | 2004-08-20 | 2006-03-02 | Carl Deirmengian | Diagnostic assay for source of inflammation |
US20060189893A1 (en) * | 2005-01-06 | 2006-08-24 | Diamics, Inc. | Systems and methods for detecting abnormal cells |
US20060161076A1 (en) * | 2005-01-06 | 2006-07-20 | Diamics, Inc. | Systems and methods for collection of cell clusters |
TWI305803B (en) * | 2005-12-05 | 2009-02-01 | Yang Chuen Yeh | A method for assaying nucleic acid fragment and kit thereof |
US20070172841A1 (en) * | 2006-01-25 | 2007-07-26 | Hui Wang | Probe/target stabilization with add-in oligo |
US20090298187A1 (en) * | 2008-04-17 | 2009-12-03 | Qiagen Gaithersburg, Inc. | Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid |
TWI419974B (zh) | 2008-10-27 | 2013-12-21 | Qiagen Gaithersburg Inc | 於自動平台上之快速結果雜交捕捉法 |
US20100216147A1 (en) * | 2009-01-28 | 2010-08-26 | Qiagen Gaithersburg, Inc. | Sequence-specific large volume sample preparation method and assay |
EP2425019B1 (de) | 2009-05-01 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | Ungezieltes verstärkungsverfahren zur erkennung von rna-spleissformen in einer probe |
JP5826752B2 (ja) | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
EP2521795B8 (de) | 2010-01-08 | 2014-01-08 | QIAGEN Gaithersburg, Inc. | Materialien und methoden zur isothermen amplifikation von nucleinsäuren |
US9605303B2 (en) | 2010-01-29 | 2017-03-28 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an HPV in a sample |
CN102822189B (zh) * | 2010-01-29 | 2016-06-29 | 奇亚根盖瑟斯堡股份有限公司 | 用于核酸的序列特异性纯化和多重复合体分析(multiplex analysis)的方法和组合物 |
JP2013528049A (ja) | 2010-05-19 | 2013-07-08 | キアゲン ガイサーズバーグ アイエヌシー. | 核酸の配列特異的な精製及び多重分析のための方法及び組成物 |
US9376727B2 (en) | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
US20120196274A1 (en) | 2011-01-07 | 2012-08-02 | Qiagen Gaithersburg, Inc. | Materials and methods for genotyping and quantifying a high-risk human papillomavirus |
CN103597095B (zh) | 2011-02-24 | 2016-08-17 | 奇亚根盖瑟斯堡股份有限公司 | 用于检测hpv核酸的材料和方法 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4732847A (en) | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
US4865980A (en) | 1982-12-29 | 1989-09-12 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
ZA853756B (en) | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
IL85551A0 (en) | 1987-04-01 | 1988-08-31 | Miles Inc | Rapid hybridization assay and reagent system used therein |
AU673813B2 (en) | 1991-11-14 | 1996-11-28 | Qiagen Gaithersburg, Inc. | Non-radioactive hybridization assay and kit |
US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
CA2139070C (en) | 1994-12-23 | 2010-03-30 | Burton W. Blais | Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction |
CA2244891C (en) | 1996-02-09 | 2008-12-30 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
US6043038A (en) | 1998-03-31 | 2000-03-28 | Tularik, Inc. | High-throughput screening assays for modulators of primase activity |
US6225053B1 (en) | 1997-12-12 | 2001-05-01 | Digene Corporation | Detection of hepatitis B virus |
DE69839572D1 (de) | 1997-12-22 | 2008-07-10 | Hitachi Chemical Co Ltd | Reinigung von mRNA auf Mikroplatten |
JP2002517981A (ja) | 1998-01-13 | 2002-06-25 | ビオヒプ テヒノロギース ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸配列を検出するための方法 |
JP2002501760A (ja) | 1998-02-02 | 2002-01-22 | アマーシャム・ファルマシア・バイオテック・アクチボラグ | 核酸解析方法 |
US5994079A (en) | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
US6232068B1 (en) | 1999-01-22 | 2001-05-15 | Rosetta Inpharmatics, Inc. | Monitoring of gene expression by detecting hybridization to nucleic acid arrays using anti-heteronucleic acid antibodies |
AU4051300A (en) | 1999-04-02 | 2000-10-23 | Tropix, Inc. | High throughput and high sensitivity detection assays |
-
2000
- 2000-11-06 US US09/707,178 patent/US6686151B1/en not_active Expired - Lifetime
- 2000-11-14 EP EP00980379A patent/EP1230396B1/de not_active Expired - Lifetime
- 2000-11-14 BR BRPI0015582A patent/BRPI0015582B1/pt not_active IP Right Cessation
- 2000-11-14 CA CA002391558A patent/CA2391558A1/en not_active Abandoned
- 2000-11-14 AT AT00980379T patent/ATE307903T1/de not_active IP Right Cessation
- 2000-11-14 AU AU17646/01A patent/AU784821B2/en not_active Ceased
- 2000-11-14 WO PCT/US2000/031277 patent/WO2001036681A2/en active IP Right Grant
- 2000-11-14 DE DE60023539T patent/DE60023539T2/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
BRPI0015582B1 (pt) | 2017-02-14 |
AU1764601A (en) | 2001-05-30 |
US6686151B1 (en) | 2004-02-03 |
CA2391558A1 (en) | 2001-05-25 |
EP1230396A2 (de) | 2002-08-14 |
BR0015582A (pt) | 2003-06-10 |
DE60023539D1 (de) | 2005-12-01 |
EP1230396B1 (de) | 2005-10-26 |
WO2001036681A2 (en) | 2001-05-25 |
AU784821B2 (en) | 2006-06-29 |
DE60023539T2 (de) | 2006-07-27 |
WO2001036681A3 (en) | 2001-12-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |