DE60023539D1 - Immunologischer nachweis von rna:dna hybriden auf mikroarrays - Google Patents

Immunologischer nachweis von rna:dna hybriden auf mikroarrays

Info

Publication number
DE60023539D1
DE60023539D1 DE60023539T DE60023539T DE60023539D1 DE 60023539 D1 DE60023539 D1 DE 60023539D1 DE 60023539 T DE60023539 T DE 60023539T DE 60023539 T DE60023539 T DE 60023539T DE 60023539 D1 DE60023539 D1 DE 60023539D1
Authority
DE
Germany
Prior art keywords
biological molecules
rna
hybrid
dna hybrid
microarrays
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60023539T
Other languages
English (en)
Other versions
DE60023539T2 (de
Inventor
G Lazar
M Zakel
M Strange
R Williams
T Lorincz
La Rosa Abel De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen Gaithersburg LLC
Original Assignee
Qiagen Gaithersburg LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/440,419 external-priority patent/US6277579B1/en
Application filed by Qiagen Gaithersburg LLC filed Critical Qiagen Gaithersburg LLC
Application granted granted Critical
Publication of DE60023539D1 publication Critical patent/DE60023539D1/de
Publication of DE60023539T2 publication Critical patent/DE60023539T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60023539T 1999-11-15 2000-11-14 Immunologischer nachweis von rna:dna-hybriden auf mikroarrays Expired - Lifetime DE60023539T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US440419 1995-05-12
US09/440,419 US6277579B1 (en) 1998-02-06 1999-11-15 Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US707178 2000-11-06
US09/707,178 US6686151B1 (en) 1998-02-06 2000-11-06 Immunological detection of RNA:DNA hybrids on microarrays
PCT/US2000/031277 WO2001036681A2 (en) 1999-11-15 2000-11-14 Immunological detection of rna:dna hybrids on microarrays

Publications (2)

Publication Number Publication Date
DE60023539D1 true DE60023539D1 (de) 2005-12-01
DE60023539T2 DE60023539T2 (de) 2006-07-27

Family

ID=27032416

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60023539T Expired - Lifetime DE60023539T2 (de) 1999-11-15 2000-11-14 Immunologischer nachweis von rna:dna-hybriden auf mikroarrays

Country Status (8)

Country Link
US (1) US6686151B1 (de)
EP (1) EP1230396B1 (de)
AT (1) ATE307903T1 (de)
AU (1) AU784821B2 (de)
BR (1) BRPI0015582B1 (de)
CA (1) CA2391558A1 (de)
DE (1) DE60023539T2 (de)
WO (1) WO2001036681A2 (de)

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US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9777312B2 (en) 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
AU2002348289A1 (en) * 2001-11-19 2003-06-10 Protometrix, Inc. Method of using a non-antibody protein to detect and measure an analyte
EP1507148B1 (de) * 2003-08-25 2005-02-23 MTM Laboratories AG Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben
US20040067492A1 (en) * 2002-10-04 2004-04-08 Allan Peng Reverse transcription on microarrays
US7560232B2 (en) * 2002-12-19 2009-07-14 Promega Corporation Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein
JP3920223B2 (ja) * 2003-01-07 2007-05-30 日本碍子株式会社 反応性チップと、このチップを用いた標的物質の結合検出方法
CA2531123A1 (en) * 2003-07-02 2005-01-13 Perkinelmer Las, Inc. Assay and process for labeling and detection of micro rna and small interfering rna sequences
US7964344B2 (en) * 2003-09-17 2011-06-21 Canon Kabushiki Kaisha Stable hybrid
AU2004286235B2 (en) 2003-10-21 2011-05-12 Orion Genomics Llc Differential enzymatic fragmentation
US7598080B2 (en) * 2004-08-20 2009-10-06 Carl Deirmengian Diagnostic assay for source of inflammation
US20060189893A1 (en) * 2005-01-06 2006-08-24 Diamics, Inc. Systems and methods for detecting abnormal cells
US20060161076A1 (en) * 2005-01-06 2006-07-20 Diamics, Inc. Systems and methods for collection of cell clusters
TWI305803B (en) * 2005-12-05 2009-02-01 Yang Chuen Yeh A method for assaying nucleic acid fragment and kit thereof
US20070172841A1 (en) * 2006-01-25 2007-07-26 Hui Wang Probe/target stabilization with add-in oligo
EP2262911B1 (de) * 2008-04-17 2016-10-12 QIAGEN Gaithersburg, Inc. Zusammensetzungen, verfahren und kits mit synthetischen sonden zum nachweis der anwesenheit einer target-nukleinsäure
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
CA2750338C (en) * 2009-01-28 2019-06-25 Qiagen Gaithersburg, Inc. Sequence-specific large volume sample preparation method and assay
JP5738278B2 (ja) 2009-05-01 2015-06-24 キアジェン ゲイサーズバーグ インコーポレイテッド 試料中のｒｎａスプライシング形態を検出するための非標的増幅法
AU2010291990B2 (en) 2009-09-14 2016-05-05 Qiagen Gaithersburg, Inc. Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media
US20120021460A1 (en) 2010-01-08 2012-01-26 Qiagen Gaithersburg Inc. Materials and methods for isothermal nucleic acid amplification
AU2011210734B2 (en) * 2010-01-29 2017-02-09 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
BR112012018545A2 (pt) 2010-01-29 2016-05-03 Qiagen Gaithersburg Inc método de determinação e confirmação da presença de um hpv em uma amostra
WO2011146629A2 (en) 2010-05-19 2011-11-24 Qiagen Gaithersburg Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
AU2011258501B2 (en) 2010-05-25 2016-07-07 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically-truncated probes
CN103517994A (zh) 2011-01-07 2014-01-15 奇亚根盖瑟斯堡股份有限公司 用于对高风险人乳头瘤病毒进行基因型分型和定量的材料和方法
US9885092B2 (en) 2011-02-24 2018-02-06 Qiagen Gaithersburg Inc. Materials and methods for detection of HPV nucleic acids

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US4732847A (en) 1981-06-09 1988-03-22 University Of Hawaii Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
US4865980A (en) 1982-12-29 1989-09-12 University Of Hawaii Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
ZA853756B (en) 1984-06-01 1986-01-29 Miles Lab Nucleic acid hybridization assay employing immobilized rna probes
IL85551A0 (en) 1987-04-01 1988-08-31 Miles Inc Rapid hybridization assay and reagent system used therein
US5981179A (en) 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
ES2145750T3 (es) 1991-11-14 2000-07-16 Dgi Inc Kit y dosificado que utiliza la hibridacion no radioactiva.
CA2139070C (en) 1994-12-23 2010-03-30 Burton W. Blais Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction
CA2244891C (en) 1996-02-09 2008-12-30 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6043038A (en) 1998-03-31 2000-03-28 Tularik, Inc. High-throughput screening assays for modulators of primase activity
US6225053B1 (en) 1997-12-12 2001-05-01 Digene Corporation Detection of hepatitis B virus
EP1865074B1 (de) 1997-12-22 2011-04-20 Hitachi Chemical Co., Ltd. Direkte RT-PCR auf Oligonukleotid-immobilisierten PCR-Mikroplatten
WO1999036571A2 (en) 1998-01-13 1999-07-22 Biochip Technologies Gmbh Method for the detection or nucleic acid of nucleic acid sequences
EP1051523A2 (de) 1998-02-02 2000-11-15 Amersham Pharmacia Biotech AB Nuklein-säure analyse verfahren
US5994079A (en) * 1998-02-06 1999-11-30 Digene Corporation Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US6232068B1 (en) 1999-01-22 2001-05-15 Rosetta Inpharmatics, Inc. Monitoring of gene expression by detecting hybridization to nucleic acid arrays using anti-heteronucleic acid antibodies
WO2000060116A1 (en) 1999-04-02 2000-10-12 Tropix, Inc. High throughput and high sensitivity detection assays

Also Published As

Publication number Publication date
DE60023539T2 (de) 2006-07-27
CA2391558A1 (en) 2001-05-25
ATE307903T1 (de) 2005-11-15
EP1230396A2 (de) 2002-08-14
AU784821B2 (en) 2006-06-29
WO2001036681A3 (en) 2001-12-13
BR0015582A (pt) 2003-06-10
AU1764601A (en) 2001-05-30
BRPI0015582B1 (pt) 2017-02-14
WO2001036681A2 (en) 2001-05-25
EP1230396B1 (de) 2005-10-26
US6686151B1 (en) 2004-02-03

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Legal Events

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Owner name: QIAGEN GAIHTERSBURG INC. (N.D.GES.D. STAATES D, US