ES2145750T3 - Kit y dosificado que utiliza la hibridacion no radioactiva. - Google Patents
Kit y dosificado que utiliza la hibridacion no radioactiva.Info
- Publication number
- ES2145750T3 ES2145750T3 ES92924354T ES92924354T ES2145750T3 ES 2145750 T3 ES2145750 T3 ES 2145750T3 ES 92924354 T ES92924354 T ES 92924354T ES 92924354 T ES92924354 T ES 92924354T ES 2145750 T3 ES2145750 T3 ES 2145750T3
- Authority
- ES
- Spain
- Prior art keywords
- kit
- dosage
- radioactive hybridization
- radioactive
- hybrid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Communicable Diseases (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
UNA PRUEBA DE HIBRIDIZACION NO RADIOACTIVA Y EQUIPO PARA LA DETECCION DE DEFECTOS GENETICOS, INFECCIONES MICROBIANAS O INFECCIONES VIRALES, TAL COMO EL VIRUS PAPILOMA HUMANO. UNA MUESTRA DE ENSAYO ES TRATADA CON UNA BASE E INCUBADA CON MUESTRAS DE ACIDO NUCLEICO, DILUIDAS EN UN REGULADOR DE NEUTRALIZACION, ESPECIFICO PARA ACIDOS NUCLEICO OBJETIVO. LOS HIBRIDOS SON CAPTURADOS EN UNA FASE SOLIDA REVESTIDA CON UN ANTICUERPO ANTIHIBRIDO, ES ELIMINADA LA MUESTRA NO HIBRIDIZADA Y DETECTADO EL HIBRIDO DETERMINADO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US79258591A | 1991-11-14 | 1991-11-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2145750T3 true ES2145750T3 (es) | 2000-07-16 |
Family
ID=25157398
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92924354T Expired - Lifetime ES2145750T3 (es) | 1991-11-14 | 1992-11-12 | Kit y dosificado que utiliza la hibridacion no radioactiva. |
Country Status (11)
Country | Link |
---|---|
US (2) | US6228578B1 (es) |
EP (1) | EP0667918B1 (es) |
JP (1) | JP3091492B2 (es) |
AT (1) | ATE189831T1 (es) |
AU (1) | AU673813B2 (es) |
DE (1) | DE69230693T2 (es) |
DK (1) | DK0667918T3 (es) |
ES (1) | ES2145750T3 (es) |
FI (1) | FI112095B (es) |
GR (1) | GR3033345T3 (es) |
WO (1) | WO1993010263A1 (es) |
Families Citing this family (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5981179A (en) * | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
US6225053B1 (en) * | 1997-12-12 | 2001-05-01 | Digene Corporation | Detection of hepatitis B virus |
BR9814272A (pt) * | 1997-12-12 | 2000-10-03 | Digene Corp | Meio de coleta universal |
US7399589B2 (en) | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US6686151B1 (en) * | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US5994079A (en) | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
DE69929826D1 (de) * | 1998-11-23 | 2006-04-20 | Us Gov Sec Army | Reinigungsmethode und vorrichtung |
US6723505B1 (en) * | 1999-08-13 | 2004-04-20 | Nye Colifast As | Method for identification of the indicators of contamination in liquid samples |
US20040115692A1 (en) * | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
US7494817B2 (en) * | 2000-09-06 | 2009-02-24 | Transnet Yx, Inc. | Methods for genotype screening using magnetic particles |
DK1337547T3 (da) * | 2000-11-03 | 2014-06-10 | Isis Pharmaceuticals Inc | Nuklease-baseret fremgangsmåde til detektion og kvantificering af oligonukleotider |
US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
US20050123913A1 (en) * | 2001-08-30 | 2005-06-09 | Emory University | Human mitochondrial dna polymorphisms, haplogroups, associations with physiological conditions, and genotyping arrays |
CA2356540A1 (en) * | 2001-08-30 | 2003-02-28 | Emory University | Expressed dna sequences involved in mitochondrial functions |
KR20030049802A (ko) * | 2001-12-17 | 2003-06-25 | 권윤정 | 바이오 칩에서 프로브와 시료 핵산의 혼성화 여부를 효소반응으로 구별하는 방법 |
US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
US20030175828A1 (en) * | 2002-03-15 | 2003-09-18 | Lazar James G. | Signal amplification by Hybrid Capture |
US20030224370A1 (en) * | 2002-05-31 | 2003-12-04 | William Rassman | Method and apparatus for recognizing molecular compounds |
HUE033832T2 (en) | 2002-11-15 | 2018-01-29 | Idenix Pharmaceuticals Llc | 2'-methyl nucleosides in combination with interferon and Flaviviridae mutation |
JP2007528692A (ja) * | 2003-05-28 | 2007-10-18 | メイバン・テクノロジーズ・エルエルシー | 分子化合物を認識する方法及び装置 |
US20060189893A1 (en) * | 2005-01-06 | 2006-08-24 | Diamics, Inc. | Systems and methods for detecting abnormal cells |
US20060161076A1 (en) * | 2005-01-06 | 2006-07-20 | Diamics, Inc. | Systems and methods for collection of cell clusters |
WO2006096727A2 (en) * | 2005-03-07 | 2006-09-14 | Cellay Llc | Methods for detecting integrated dna |
US20070037174A1 (en) * | 2005-08-12 | 2007-02-15 | Rampal Jang B | Chemiluminescent generated fluorescent labeling |
US7972776B2 (en) * | 2005-11-15 | 2011-07-05 | Oncohealth Corporation | Protein chips for HPV detection |
US7732166B2 (en) * | 2005-11-15 | 2010-06-08 | Oncohealth Corporation | Detection method for human pappilomavirus (HPV) and its application in cervical cancer |
US20080125333A1 (en) * | 2006-05-31 | 2008-05-29 | Antara Biosciences Inc. | Devices and kits for detecting one or more target agents |
US8968995B2 (en) * | 2008-11-12 | 2015-03-03 | Oncohealth Corp. | Detection, screening, and diagnosis of HPV-associated cancers |
US20100003704A1 (en) * | 2008-06-13 | 2010-01-07 | Shuling Cheng | IN SITU detection of early stages and late stages HPV infection |
JP2010520747A (ja) * | 2007-02-23 | 2010-06-17 | パナジェン インコーポレイテッド | 支持体に固定されたpnaプローブと標的核酸の混成化効率又は特異度を増加させるための方法、組成物、及びキット |
JP2011518333A (ja) * | 2008-04-17 | 2011-06-23 | キアジェン ゲイサーズバーグ インコーポレイテッド | 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット |
DK2279265T3 (da) | 2008-04-24 | 2014-12-08 | Newsouth Innovations Pty Ltd | Cyanobakterie-saxitoxin-gencluster og detektering af cyanotoksiske organismer |
US8288520B2 (en) * | 2008-10-27 | 2012-10-16 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and system |
WO2010078574A1 (en) | 2009-01-05 | 2010-07-08 | Afl Telecommunications Llc | Universal mounting bracket |
JP6108661B2 (ja) * | 2009-01-28 | 2017-04-05 | キアジェン ゲイサーズバーグ インコーポレイテッド | 配列特異的な大量試料調製方法およびアッセイ法 |
CA2760542A1 (en) | 2009-05-01 | 2010-11-04 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
JP2012526286A (ja) | 2009-05-07 | 2012-10-25 | オンコヘルス コーポレーション | ヒトパピローマウイルス(hpv)およびhpv関連癌の初期段階および後期段階の検出、スクリーニング、および診断のための高度または≧cin2の同定 |
EP2478087B1 (en) | 2009-09-14 | 2017-01-18 | QIAGEN Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
WO2011084598A1 (en) | 2010-01-08 | 2011-07-14 | Oncohealth Corporation | High throughput cell-based hpv immunoassays for diagnosis and screening of hpv-associated cancers |
BR112012018545A2 (pt) * | 2010-01-29 | 2016-05-03 | Qiagen Gaithersburg Inc | método de determinação e confirmação da presença de um hpv em uma amostra |
WO2011094514A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
DK2539355T3 (da) * | 2010-02-26 | 2017-01-02 | Ventana Med Syst Inc | IN-SITU-hybridisering med polytag-prober |
JP2013528049A (ja) | 2010-05-19 | 2013-07-08 | キアゲン ガイサーズバーグ アイエヌシー. | 核酸の配列特異的な精製及び多重分析のための方法及び組成物 |
US9376727B2 (en) | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
CA2828224A1 (en) | 2011-02-24 | 2012-08-30 | Qiagen Gaithersburg, Inc. | Materials and methods for detection of hpv nucleic acids |
EP2773745B1 (en) | 2011-11-03 | 2019-04-10 | QIAGEN Gaithersburg, Inc. | Materials and method for immobilizing, isolating, and concentrating cells using carboxylated surfaces |
GB2559919B (en) | 2014-02-28 | 2018-11-14 | Gen Probe Inc | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
CN111484992B (zh) | 2014-02-28 | 2024-04-02 | 简·探针公司 | 从含有甲醛的液基细胞学防腐剂中的样本分离核酸的方法 |
US10356478B2 (en) * | 2015-01-08 | 2019-07-16 | The Directv Group, Inc. | Systems and methods for spotted advertising and control of corresponding user interfaces and transactions via user receiving devices and mobile devices |
JP7056143B2 (ja) * | 2016-12-27 | 2022-04-19 | 東洋紡株式会社 | 免疫学的測定用組成物および免疫学的測定方法 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4732847A (en) | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
US4623627A (en) | 1983-08-19 | 1986-11-18 | Cetus Corporation | Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same |
EP0146039A3 (en) * | 1983-12-12 | 1986-08-27 | Miles Inc. | Hybridization assay with immobilization of hybrids by antihybrid binding |
US4743535A (en) | 1984-11-07 | 1988-05-10 | Miles Inc. | Hybridization assay employing labeled probe and anti-hybrid |
ZA853756B (en) | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
CA1253777A (en) | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Nucleic acid hybridization assay employing immobilized rna probes |
US4563417A (en) | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
US4965188A (en) * | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
AU558846B2 (en) * | 1985-06-21 | 1987-02-12 | Miles Laboratories Inc. | Solid-phase hydridization assay using anti-hybrid antibodies |
US4968602A (en) * | 1986-03-05 | 1990-11-06 | Molecular Diagnostics, Inc. | Solution-phase single hybridization assay for detecting polynucleotide sequences |
GB8712646D0 (en) * | 1987-05-29 | 1987-07-01 | Clayton Found Res | Structural evidence |
EP0377573A1 (en) * | 1987-07-22 | 1990-07-18 | Imperial Cancer Research Technology Limited | Digestion method |
-
1992
- 1992-11-12 DE DE69230693T patent/DE69230693T2/de not_active Expired - Lifetime
- 1992-11-12 ES ES92924354T patent/ES2145750T3/es not_active Expired - Lifetime
- 1992-11-12 AT AT92924354T patent/ATE189831T1/de active
- 1992-11-12 DK DK92924354T patent/DK0667918T3/da active
- 1992-11-12 AU AU30689/92A patent/AU673813B2/en not_active Expired
- 1992-11-12 WO PCT/US1992/009604 patent/WO1993010263A1/en active IP Right Grant
- 1992-11-12 JP JP05509340A patent/JP3091492B2/ja not_active Expired - Lifetime
- 1992-11-12 EP EP92924354A patent/EP0667918B1/en not_active Expired - Lifetime
-
1994
- 1994-01-18 US US08/183,154 patent/US6228578B1/en not_active Expired - Lifetime
- 1994-05-13 FI FI942239A patent/FI112095B/fi not_active IP Right Cessation
-
2000
- 2000-04-26 GR GR20000401030T patent/GR3033345T3/el unknown
-
2001
- 2001-05-07 US US09/850,041 patent/US20020012936A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP0667918B1 (en) | 2000-02-16 |
US6228578B1 (en) | 2001-05-08 |
DK0667918T3 (da) | 2000-06-05 |
WO1993010263A1 (en) | 1993-05-27 |
US20020012936A1 (en) | 2002-01-31 |
EP0667918A1 (en) | 1995-08-23 |
ATE189831T1 (de) | 2000-03-15 |
JP3091492B2 (ja) | 2000-09-25 |
AU673813B2 (en) | 1996-11-28 |
FI112095B (fi) | 2003-10-31 |
FI942239A0 (fi) | 1994-05-13 |
DE69230693T2 (de) | 2000-06-15 |
DE69230693D1 (de) | 2000-03-23 |
JPH07505759A (ja) | 1995-06-29 |
FI942239A (fi) | 1994-07-06 |
GR3033345T3 (en) | 2000-09-29 |
AU3068992A (en) | 1993-06-15 |
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