ATE364722T1 - Verfahren zum nachweis und zur lokalisierung von genen in situ durch hybridisierung einer verzweigten dna - Google Patents

Verfahren zum nachweis und zur lokalisierung von genen in situ durch hybridisierung einer verzweigten dna

Info

Publication number
ATE364722T1
ATE364722T1 AT01941737T AT01941737T ATE364722T1 AT E364722 T1 ATE364722 T1 AT E364722T1 AT 01941737 T AT01941737 T AT 01941737T AT 01941737 T AT01941737 T AT 01941737T AT E364722 T1 ATE364722 T1 AT E364722T1
Authority
AT
Austria
Prior art keywords
detecting
nucleic acid
situ
hybridizing
acid analyte
Prior art date
Application number
AT01941737T
Other languages
English (en)
Inventor
Daryn Kenny
Lu Shen
Vincent Antao
Audrey Player
Wei Cao
Original Assignee
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Corp filed Critical Bayer Corp
Application granted granted Critical
Publication of ATE364722T1 publication Critical patent/ATE364722T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT01941737T 2000-06-02 2001-06-01 Verfahren zum nachweis und zur lokalisierung von genen in situ durch hybridisierung einer verzweigten dna ATE364722T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US20913900P 2000-06-02 2000-06-02

Publications (1)

Publication Number Publication Date
ATE364722T1 true ATE364722T1 (de) 2007-07-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AT01941737T ATE364722T1 (de) 2000-06-02 2001-06-01 Verfahren zum nachweis und zur lokalisierung von genen in situ durch hybridisierung einer verzweigten dna

Country Status (7)

Country Link
US (1) US7033758B2 (de)
EP (1) EP1287165B1 (de)
JP (1) JP2003535600A (de)
AT (1) ATE364722T1 (de)
DE (1) DE60128908T2 (de)
ES (1) ES2287134T3 (de)
WO (1) WO2001094632A2 (de)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005090608A2 (en) * 2004-03-05 2005-09-29 Advandx, Inc. High affinity probes for analysis of human papillomavirus expression
WO2005098049A2 (en) * 2004-03-25 2005-10-20 California Institute Of Technology Hybridization chain reaction
US20060234261A1 (en) * 2005-03-08 2006-10-19 Pierce Niles A Colorimetric readout of hybridization chain reaction
US7727721B2 (en) * 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
CA2606723A1 (en) 2005-05-09 2006-11-16 Panomics, Inc. Multiplex capture of nucleic acids
US7803541B2 (en) 2005-05-12 2010-09-28 Panomics, Inc. Multiplex branched-chain DNA assays
EP3042963A1 (de) * 2005-06-20 2016-07-13 Advanced Cell Diagnostics, Inc. Verfahren zum nachweis von nukleinsäuren in einzelnen zellen und zur identifikation seltener zellen aus grossen heterogenen zellpopulationen
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
WO2007044427A2 (en) * 2005-10-05 2007-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
US7960357B2 (en) * 2005-10-07 2011-06-14 California Institute Of Technology PKR activation via hybridization chain reaction
ATE553211T1 (de) 2005-11-10 2012-04-15 Affymetrix Inc Nachweis von nukleinsäuren über amplifikation von stellvertreternukleinsäuren
JP2008043332A (ja) * 2006-08-17 2008-02-28 Panomics Inc 組織スライドからの核酸定量
WO2008069884A2 (en) * 2006-12-01 2008-06-12 Panomics, Inc. Two-stage nucleic acid amplification using an amplification oligomer
US8318921B2 (en) * 2007-03-01 2012-11-27 California Institute Of Technology Triggered RNAi
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
WO2009048530A2 (en) 2007-10-05 2009-04-16 Panomics, Inc. Highly multiplexed particle-based assays
US8497364B2 (en) * 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
US20100021904A1 (en) * 2008-05-21 2010-01-28 Pierce Niles A Shielded cross-linking probes
US8241854B2 (en) * 2008-05-22 2012-08-14 California Institute Of Technology Triggered RNAi
US20100021901A1 (en) * 2008-05-22 2010-01-28 Peng Yin Compositions and methods for detecting analytes
US20090298709A1 (en) * 2008-05-28 2009-12-03 Affymetrix, Inc. Assays for determining telomere length and repeated sequence copy number
EP2358897B1 (de) * 2008-11-17 2015-02-25 Headway Technologies, Inc. Verfahren und zusammensetzungen bei dem nachweis von zielmolekülen auf partikelbasis unter verwendung von verknüpfungsmolekülen
JP5744743B2 (ja) * 2008-11-17 2015-07-08 ヘッドウェイ テクノロジーズ, インク.Headway Technologies, Inc. 共有結合形成反応ペアを用いた標的分子の粒子ベースの検出における方法および組成物
US20130023433A1 (en) 2009-09-28 2013-01-24 Yuling Luo Methods of detecting nucleic acid sequences with high specificity
WO2011082293A1 (en) 2009-12-31 2011-07-07 Ventana Medical Systems, Inc. Methods for producing uniquely specific nucleic acid probes
US20130171621A1 (en) * 2010-01-29 2013-07-04 Advanced Cell Diagnostics Inc. Methods of in situ detection of nucleic acids
CA2787487C (en) * 2010-02-26 2018-07-24 Ventana Medical Systems, Inc. Polytag probes
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
US9512471B2 (en) * 2010-06-30 2016-12-06 Diacarta Inc Methods and kits for detecting human papillomavirus
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US9938590B2 (en) * 2010-09-16 2018-04-10 Gen-Probe Incorporated Capture probes immobilizable via L-nucleotide tail
US20140031250A1 (en) 2010-10-07 2014-01-30 David Tsai Ting Biomarkers of Cancer
CN107365847A (zh) 2010-10-21 2017-11-21 领先细胞医疗诊断有限公司 用于原位检测核酸的超灵敏方法
CN103328654B (zh) 2010-10-27 2017-07-11 哈佛学院院长等 立足点引物双链体的组合物及其使用方法
US20120214152A1 (en) * 2011-01-28 2012-08-23 Xiao-Jun Ma Rnascope® hpv assay for determining hpv status in head and neck cancers and cervical lesions
US9447455B2 (en) 2011-02-16 2016-09-20 Headway Technologies, Inc. Methods and compositions for the target-localized anchoring of detectable label
EP3604555A1 (de) 2011-10-14 2020-02-05 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
EP4108782B1 (de) 2011-12-22 2023-06-07 President and Fellows of Harvard College Zusammensetzungen und verfahren zur analytdetektion
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2013167387A1 (en) 2012-05-10 2013-11-14 Ventana Medical Systems, Inc. Uniquely specific probes for pten, pik3ca, met, top2a, and mdm2
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
WO2014048942A1 (en) 2012-09-25 2014-04-03 Ventana Medical Systems, Inc. Probes for pten, pik3ca, met, and top2a, and method for using the probes
US9783841B2 (en) * 2012-10-04 2017-10-10 The Board Of Trustees Of The Leland Stanford Junior University Detection of target nucleic acids in a cellular sample
EP2971184B1 (de) 2013-03-12 2019-04-17 President and Fellows of Harvard College Verfahren zur erzeugung einer dreidimensionalen nukleinsäure mit matrix
US9273349B2 (en) 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
KR102368591B1 (ko) 2013-04-30 2022-02-25 캘리포니아 인스티튜트 오브 테크놀로지 순차적 하이브리드화 바코딩에 의한 분자의 멀티플렉스 표지화
EP3003392B1 (de) 2013-06-04 2019-10-23 President and Fellows of Harvard College Rna-geführte transkriptionsregulation
US9856472B2 (en) 2013-07-01 2018-01-02 California Institute Of Technology Small conditional RNAs
US20150105298A1 (en) * 2013-10-10 2015-04-16 The Research Foundation For The State University Of New York Multi-oligomer in situ hybridization probes
US20150247204A1 (en) * 2014-02-07 2015-09-03 The General Hopital Corporation Differential diagnosis of hepatic neoplasms
WO2015123565A1 (en) 2014-02-14 2015-08-20 The General Hospital Corporation Methods for diagnosing igg4-related disease
WO2015131099A1 (en) 2014-02-28 2015-09-03 The General Hospital Corporation Diagnosis of multiple myeloma and lymphoma
US10301624B2 (en) 2014-06-25 2019-05-28 The General Hospital Corporation Targeting human satellite II (HSATII)
WO2016025867A1 (en) * 2014-08-15 2016-02-18 Affymetrix, Inc. Robust detection of nucleic acids in situ
CN107208160B (zh) 2015-01-30 2023-02-17 哈佛学院院长及董事 无显微镜成像
CN106555010A (zh) * 2015-09-30 2017-04-05 江苏博铼生技医疗科技有限公司 人类乳头瘤病毒检测试剂盒及检测方法
US11078528B2 (en) 2015-10-12 2021-08-03 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
AU2016349288A1 (en) 2015-11-03 2018-05-31 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
US10732175B2 (en) 2015-12-14 2020-08-04 The Penn State Research Foundation Polymer based signal amplification for protein and cell detection
CN116200465A (zh) * 2016-04-25 2023-06-02 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
AU2017291727B2 (en) 2016-07-05 2021-07-08 California Institute Of Technology Fractional initiator hybridization chain reaction
IL264831B (en) 2016-08-30 2022-09-01 California Inst Of Techn Immunohistochemistry by hybridization chain reaction
WO2018045186A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
CN118389650A (zh) 2016-08-31 2024-07-26 哈佛学院董事及会员团体 生成用于通过荧光原位测序检测的核酸序列文库的方法
AU2017348369B2 (en) 2016-10-27 2023-09-28 Geneoscopy, Inc. Detection method
CN110225980B (zh) 2016-11-21 2023-01-06 纳米线科技公司 化学组合物及其使用方法
WO2018112422A1 (en) * 2016-12-16 2018-06-21 Aratome, LLC Molecular detection using ligation amplification
WO2018119295A1 (en) * 2016-12-22 2018-06-28 Ventana Medical Systems, Inc. Fully automated nucleic acid extraction method for tissue samples
EP3568492A4 (de) * 2017-01-10 2021-03-17 President and Fellows of Harvard College Multiplexierte signalverstärkung
US11981956B2 (en) 2018-01-26 2024-05-14 President And Fellows Of Harvard College Proximity detection methods and compositions
CA3090652A1 (en) 2018-02-06 2019-08-15 The General Hospital Corporation Repeat rna as biomarkers of tumor immune response
CN112703255A (zh) 2018-05-14 2021-04-23 纳米线科技公司 化学组合物及其使用方法
SG11202101934SA (en) 2018-07-30 2021-03-30 Readcoor Llc Methods and systems for sample processing or analysis
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN109943665B (zh) * 2019-04-15 2023-05-16 郑州科蒂亚生物技术有限公司 一种用于检测头颈鳞癌致癌基因检测的探针序列及其试剂盒、应用
US20210340621A1 (en) * 2020-04-22 2021-11-04 Readcoor, Llc Methods and systems for sequencing
CN111378723B (zh) * 2020-04-24 2023-05-26 郑州科蒂亚生物技术有限公司 一种在组织上检测PD-L1 mRNA的试剂盒及方法
CN112831599B (zh) * 2020-12-07 2023-05-23 郑州科蒂亚生物技术有限公司 一种基于信号放大技术的CoVID-19病毒检测试剂盒
EP4284925A1 (de) 2021-01-26 2023-12-06 California Institute of Technology Allosterische bedingte guide-rnas zur zellselektiven regulierung von crispr/cas

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5849481A (en) * 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
JP3454847B2 (ja) * 1992-08-26 2003-10-06 株式会社東芝 ハイブリダイゼーション法
US5501954A (en) * 1994-06-13 1996-03-26 Genzyme Corporation Method of detecting cellular material
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US6022689A (en) * 1995-04-07 2000-02-08 University Of New Mexico Situ hybridization slide processes
US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
WO1999011813A2 (en) * 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof

Also Published As

Publication number Publication date
ES2287134T3 (es) 2007-12-16
EP1287165B1 (de) 2007-06-13
DE60128908T2 (de) 2008-02-28
EP1287165A2 (de) 2003-03-05
US20020172950A1 (en) 2002-11-21
WO2001094632A3 (en) 2002-05-30
US7033758B2 (en) 2006-04-25
DE60128908D1 (de) 2007-07-26
JP2003535600A (ja) 2003-12-02
WO2001094632A2 (en) 2001-12-13

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