ATE188745T1 - Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt - Google Patents
Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubtInfo
- Publication number
- ATE188745T1 ATE188745T1 AT96927108T AT96927108T ATE188745T1 AT E188745 T1 ATE188745 T1 AT E188745T1 AT 96927108 T AT96927108 T AT 96927108T AT 96927108 T AT96927108 T AT 96927108T AT E188745 T1 ATE188745 T1 AT E188745T1
- Authority
- AT
- Austria
- Prior art keywords
- probe
- detection
- nucleic acid
- nuclic acid
- once
- Prior art date
Links
- 239000000523 sample Substances 0.000 title abstract 6
- 238000001514 detection method Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 2
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9509290A FR2737502B1 (fr) | 1995-07-31 | 1995-07-31 | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
| PCT/FR1996/001201 WO1997005277A1 (fr) | 1995-07-31 | 1996-07-30 | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE188745T1 true ATE188745T1 (de) | 2000-01-15 |
Family
ID=9481556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96927108T ATE188745T1 (de) | 1995-07-31 | 1996-07-30 | Verfahren zur detektion einer nukleinsaeure mittels einer nukleinsaeuresonde, das ein spezifisches einfangen und eine detektion auf einmal erlaubt |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6228580B1 (de) |
| EP (1) | EP0843738B1 (de) |
| JP (1) | JP4050789B2 (de) |
| AT (1) | ATE188745T1 (de) |
| AU (1) | AU717454B2 (de) |
| CA (1) | CA2228712A1 (de) |
| DE (1) | DE69606180T2 (de) |
| FR (1) | FR2737502B1 (de) |
| WO (1) | WO1997005277A1 (de) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000033244A2 (en) * | 1998-11-27 | 2000-06-08 | Synaptics (Uk) Limited | Position sensor |
| DE19548680A1 (de) * | 1995-12-23 | 1997-06-26 | Boehringer Mannheim Gmbh | Verfahren zum quantitativen Nachweis von spezifischen Nukleinsäuresequenzen |
| US20050069923A1 (en) * | 1996-07-08 | 2005-03-31 | Mullis Kary Banks | Dual bead assays using cleavable spacers and/or ligation to improve specificity and sensitivity including related methods and apparatus |
| CA2389358C (en) | 1996-12-31 | 2008-07-15 | Genometrix Incorporated | Multiplexed molecular analysis apparatus and method |
| US20040241759A1 (en) * | 1997-06-16 | 2004-12-02 | Eileen Tozer | High throughput screening of libraries |
| US20050070005A1 (en) * | 1997-06-16 | 2005-03-31 | Martin Keller | High throughput or capillary-based screening for a bioactivity or biomolecule |
| US20030049841A1 (en) * | 1997-06-16 | 2003-03-13 | Short Jay M. | High throughput or capillary-based screening for a bioactivity or biomolecule |
| US6238869B1 (en) | 1997-12-19 | 2001-05-29 | High Throughput Genomics, Inc. | High throughput assay system |
| AU2007231625B2 (en) * | 1997-12-19 | 2011-04-07 | Stephen Felder | High Throughput Assay System |
| US20100105572A1 (en) * | 1997-12-19 | 2010-04-29 | Kris Richard M | High throughput assay system |
| US6458533B1 (en) | 1997-12-19 | 2002-10-01 | High Throughput Genomics, Inc. | High throughput assay system for monitoring ESTs |
| US6232066B1 (en) | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
| US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| AU2375600A (en) * | 1998-12-22 | 2000-07-12 | Stephen Felder | High throughput assay system using mass spectrometry |
| US6849397B2 (en) * | 1999-05-04 | 2005-02-01 | Wisconsin Alumni Research Foundation | Label-free detection of nucleic acids via surface plasmon resonance |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US6900013B1 (en) | 2000-08-25 | 2005-05-31 | Aviva Biosciences Corporation | Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization |
| AU2001285219A1 (en) * | 2000-08-24 | 2002-03-04 | Aviva Biosciences Corporation | Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization |
| ATE380883T1 (de) * | 2000-10-24 | 2007-12-15 | Univ Leland Stanford Junior | Direkte multiplex charakterisierung von genomischer dna |
| CA2446748C (en) * | 2001-05-11 | 2011-03-08 | University Of Ottawa | Anxiolytic marcgraviaceae compositions containing betulinic acid, betulinic acid derivatives, and methods |
| US7083920B2 (en) * | 2001-05-18 | 2006-08-01 | Nagaoka & Co. Ltd. | Surface assembly for immobilizing DNA capture probes in genetic assays using enzymatic reactions to generate signal in optical bio-discs and methods relating thereto |
| WO2003016574A1 (en) * | 2001-08-16 | 2003-02-27 | Zhifeng Shao | Analysis of gene expression profiles using sequential hybridization |
| KR20030049802A (ko) * | 2001-12-17 | 2003-06-25 | 권윤정 | 바이오 칩에서 프로브와 시료 핵산의 혼성화 여부를 효소반응으로 구별하는 방법 |
| US20030175709A1 (en) * | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
| US20050019901A1 (en) * | 2002-01-31 | 2005-01-27 | Evgenia Matveeva | Methods for synthesis of bio-active nanoparticles and nanocapsules for use in optical bio-disc assays and disc assembly including same |
| KR20020041344A (ko) * | 2002-02-26 | 2002-06-01 | 김희태 | 멤브레인 크로마토그래프 기법을 활용하여핵산중합반응으로 증폭된 핵산을 검출하는 방법 |
| KR100492393B1 (ko) * | 2002-12-02 | 2005-05-30 | 굿젠 주식회사 | 멤브레인 크로마토그래프 기법에 의한 핵산 검출 방법 및이를 위한 킷트 |
| US20040265883A1 (en) * | 2003-06-27 | 2004-12-30 | Biocept, Inc. | mRNA expression analysis |
| US7294462B2 (en) * | 2004-03-17 | 2007-11-13 | Japan Software Management Co., Ltd. | Method for detection of base sequence of interest |
| EP1580280A1 (de) * | 2004-03-23 | 2005-09-28 | Japan Software Management Co., Ltd. | Methode zur Detektion von Basensequenzen. |
| ATE452208T1 (de) * | 2004-10-08 | 2010-01-15 | Sysmex Corp | Verfahren zum nachweis einer zielsubstanz unter verwendung einer nukleinsäuresonde |
| US20090264635A1 (en) * | 2005-03-25 | 2009-10-22 | Applera Corporation | Methods and compositions for depleting abundant rna transcripts |
| WO2007050990A2 (en) * | 2005-10-27 | 2007-05-03 | Rosetta Inpharmatics Llc | Nucleic acid amplification using non-random primers |
| US8137936B2 (en) * | 2005-11-29 | 2012-03-20 | Macevicz Stephen C | Selected amplification of polynucleotides |
| DE602006018352D1 (de) | 2005-12-06 | 2010-12-30 | Ambion Inc | Rückübertragungs-primer und verfahren zu deren entwurf |
| JP2009268665A (ja) * | 2008-05-07 | 2009-11-19 | Canon Inc | 吸入装置 |
| JP2012506705A (ja) | 2008-10-27 | 2012-03-22 | キアジェン ゲイサーズバーグ インコーポレイテッド | 迅速に結果を得るハイブリッドキャプチャー法による分析およびシステム |
| CA2750338C (en) * | 2009-01-28 | 2019-06-25 | Qiagen Gaithersburg, Inc. | Sequence-specific large volume sample preparation method and assay |
| CN102414327B (zh) * | 2009-05-01 | 2015-04-08 | 奇亚根盖瑟斯堡股份有限公司 | 用于在样品中检测rna剪接形式的非靶向扩增方法 |
| WO2011032124A1 (en) | 2009-09-14 | 2011-03-17 | Qiagen Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| JP6103941B2 (ja) | 2010-01-29 | 2017-03-29 | キアジェン ゲイサーズバーグ インコーポレイテッド | 核酸の配列特異的精製および多重分析のための方法および組成物 |
| JP2013517803A (ja) | 2010-01-29 | 2013-05-20 | キアジェン ゲイサーズバーグ インコーポレイテッド | サンプル中のhpvの存在を決定および確認する方法 |
| CA2799200A1 (en) | 2010-05-19 | 2011-11-24 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| US9376727B2 (en) | 2010-05-25 | 2016-06-28 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically truncated probes |
| WO2012116220A2 (en) | 2011-02-24 | 2012-08-30 | Qiagen Gaithersburg, Inc. | Materials and methods for detection of hpv nucleic acid |
| AU2012316129B2 (en) * | 2011-09-28 | 2017-10-05 | Htg Molecular Diagnostics, Inc. | Methods of co-detecting mRNA and small non-coding RNA |
| CN214973877U (zh) | 2019-08-09 | 2021-12-03 | 胡桃钳医疗公司 | 用于形成治疗性多核苷酸的微流体设备及微流体路径装置 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4775619A (en) * | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US5629158A (en) * | 1989-03-22 | 1997-05-13 | Cemu Bitecknik Ab | Solid phase diagnosis of medical conditions |
| JP2745816B2 (ja) * | 1990-05-07 | 1998-04-28 | ダイキン工業株式会社 | ダブルdループ形成の診断応用 |
| CA2119701A1 (en) | 1993-04-16 | 1994-10-17 | Colleen M. Nycz | Nucleic acid assay procedure |
| CA2139623A1 (en) * | 1993-05-06 | 1994-11-24 | Baxter Diagnostics Inc. | Human papillomavirus detection assay |
| CA2125344A1 (en) * | 1993-07-01 | 1995-01-02 | Casey D. Morrow | Encapsidated recombinant poliovirus nucleic acid and methods of making and using same |
| NZ275286A (en) * | 1993-10-22 | 1998-08-26 | Amgen Inc | Detecting target nucleic acid sequences using labelled rna probe hybridization and rnase digestion |
| US5589329A (en) * | 1993-11-15 | 1996-12-31 | Ambion, Inc. | Methods and compositions for detecting base pair mismatches |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5786203A (en) * | 1994-06-14 | 1998-07-28 | Neurocrine Biosciences, Inc. | Isolated nucleic acid encoding corticotropin-releasing factor2 receptors |
-
1995
- 1995-07-31 FR FR9509290A patent/FR2737502B1/fr not_active Expired - Fee Related
-
1996
- 1996-07-30 WO PCT/FR1996/001201 patent/WO1997005277A1/fr not_active Ceased
- 1996-07-30 DE DE69606180T patent/DE69606180T2/de not_active Expired - Fee Related
- 1996-07-30 AT AT96927108T patent/ATE188745T1/de not_active IP Right Cessation
- 1996-07-30 US US09/000,384 patent/US6228580B1/en not_active Expired - Fee Related
- 1996-07-30 EP EP96927108A patent/EP0843738B1/de not_active Expired - Lifetime
- 1996-07-30 AU AU67045/96A patent/AU717454B2/en not_active Ceased
- 1996-07-30 CA CA002228712A patent/CA2228712A1/fr not_active Abandoned
- 1996-07-30 JP JP50729297A patent/JP4050789B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| FR2737502B1 (fr) | 1997-10-24 |
| DE69606180D1 (de) | 2000-02-17 |
| JP4050789B2 (ja) | 2008-02-20 |
| EP0843738A1 (de) | 1998-05-27 |
| CA2228712A1 (fr) | 1997-02-13 |
| JPH11514850A (ja) | 1999-12-21 |
| WO1997005277A1 (fr) | 1997-02-13 |
| AU6704596A (en) | 1997-02-26 |
| AU717454B2 (en) | 2000-03-23 |
| US6228580B1 (en) | 2001-05-08 |
| EP0843738B1 (de) | 2000-01-12 |
| DE69606180T2 (de) | 2000-09-28 |
| FR2737502A1 (fr) | 1997-02-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |