HUP0101385A2 - Eljárás nukleinsavak detektálására PCR-módszerrel - Google Patents

Eljárás nukleinsavak detektálására PCR-módszerrel

Info

Publication number
HUP0101385A2
HUP0101385A2 HU0101385A HUP0101385A HUP0101385A2 HU P0101385 A2 HUP0101385 A2 HU P0101385A2 HU 0101385 A HU0101385 A HU 0101385A HU P0101385 A HUP0101385 A HU P0101385A HU P0101385 A2 HUP0101385 A2 HU P0101385A2
Authority
HU
Hungary
Prior art keywords
sample
fluorescently labeled
fluorescence
probe
detection
Prior art date
Application number
HU0101385A
Other languages
English (en)
Inventor
Martin Alan Lee
Dario Lyall Leslie
Original Assignee
The Secretary Of State For Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Secretary Of State For Defence filed Critical The Secretary Of State For Defence
Publication of HUP0101385A2 publication Critical patent/HUP0101385A2/hu
Publication of HUP0101385A3 publication Critical patent/HUP0101385A3/hu
Publication of HU226518B1 publication Critical patent/HU226518B1/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/80—Elimination or reduction of contamination by undersired ferments, e.g. aseptic cultivation

Abstract

A találmány tárgya eljárás egy célzott nukleinsav-szekvencia jelenlétének detektálására egy mintában, amely abban áll,hogy (a) az említett mintát amplifikációs reakciónak vetik alánukleotidok egy sorozatának felhasználásával, amelyben legalább azegyik fluoreszcensen jelzett, (b) az amplifikációs terméket egypróbával érintkeztetik olyan körülmények között, amelyben a próbahibridizálni fog a cél szekvenciához, továbbá az említett próba egyreaktív molekulát tartalmaz, amely képes fluoreszcenciát abszorbeálniaz említett fluoreszcensen jelzett nukleotidból vagy fluoreszcensenergiát átadni az említett fluoreszcensen jelzett nukleotidnak, és(c) nyomon követik az említett minta fluoreszcenciáját. A találmányszerinti eljárás felhasználható egy célzott nukleinsav, mennyiségénekmeghatározására egy mintában, valamint szekvencia jellemzőkkimutatására. Az eljárás megvalósítására szolgáló kitek szintén atalálmány tárgyát képezik. Ó
HU0101385A 1998-02-19 1999-02-18 Method for detection of target nucleic acids using pcr HU226518B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9803382.2A GB9803382D0 (en) 1998-02-19 1998-02-19 Detection system
PCT/GB1999/000504 WO1999042611A1 (en) 1998-02-19 1999-02-18 Method for detection of target nucleic acids using pcr

Publications (3)

Publication Number Publication Date
HUP0101385A2 true HUP0101385A2 (hu) 2001-08-28
HUP0101385A3 HUP0101385A3 (en) 2003-08-28
HU226518B1 HU226518B1 (en) 2009-03-30

Family

ID=10827164

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0101385A HU226518B1 (en) 1998-02-19 1999-02-18 Method for detection of target nucleic acids using pcr

Country Status (16)

Country Link
US (1) US6287781B1 (hu)
EP (1) EP1055002B1 (hu)
JP (1) JP2003512808A (hu)
CN (1) CN1203186C (hu)
AT (1) ATE398187T1 (hu)
AU (1) AU746149B2 (hu)
CA (1) CA2321185C (hu)
CZ (1) CZ20002958A3 (hu)
DE (1) DE69938894D1 (hu)
GB (4) GB9803382D0 (hu)
HU (1) HU226518B1 (hu)
IL (1) IL137909A0 (hu)
NZ (1) NZ506333A (hu)
RU (1) RU2199588C2 (hu)
SK (1) SK12212000A3 (hu)
WO (1) WO1999042611A1 (hu)

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GB0111275D0 (en) 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
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US20060172293A1 (en) * 2002-05-31 2006-08-03 Kankyo Engineering Co, Ltd. Novel method of assaying nucleic acid using labeled nucleotide
US7183104B1 (en) 2002-08-23 2007-02-27 Duane Morris Llp Separator and particle detection system
US7074598B2 (en) * 2002-09-25 2006-07-11 Mayo Foundation For Medical Education And Research Detection of vancomycin-resistant enterococcus spp.
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GB0503172D0 (en) 2005-02-16 2005-03-23 Enigma Diagnostics Ltd Detection method
GB0517005D0 (en) 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
RU2005132940A (ru) * 2006-03-01 2007-11-27 Институт биохимии и генетики Уфимского научного центра РАН (RU) Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени
EP2759307B1 (en) 2006-03-29 2016-11-09 Merial Limited Vaccine against Streptococci
GB0806676D0 (en) 2008-04-12 2008-05-14 Environment Agency The Environmental monitoring
EP2116614A1 (en) * 2008-05-06 2009-11-11 Qiagen GmbH Simultaneous detection of multiple nucleic acid sequences in a reaction
EP2138588A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Melting curve measurement during amplification
US20110045458A1 (en) * 2009-08-20 2011-02-24 Mayo Foundation For Medical Education And Research Detection of Enterovirus
GB0915664D0 (en) 2009-09-08 2009-10-07 Enigma Diagnostics Ltd Reaction method
RU2585519C2 (ru) * 2012-11-28 2016-05-27 ЗАО "Геноаналитика" Способ селективного отбора целевых участков днк
AU2015319825A1 (en) * 2014-09-26 2017-04-27 Nooma Bio, Inc. Target sequence detection by nanopore sensing of synthetic probes
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
RU2665631C2 (ru) * 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
CN108642150B (zh) * 2018-05-18 2023-12-08 阅尔基因技术（苏州）有限公司 一种基于fret的荧光核酸检测方法和试剂盒
US11604186B2 (en) * 2018-10-17 2023-03-14 Molecular Devices (Austria) GmbH Real time western blot assays utilizing fluorescence resonance energy transfer (FRET)
JP2022510063A (ja) 2018-12-21 2022-01-26 イルミナ インコーポレイテッド 感知システム
CN109576350B (zh) * 2019-01-18 2021-01-29 深圳恒特基因有限公司 一种dna与rna同时定量的试剂盒、方法及质控方法

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Also Published As

Publication number Publication date
CZ20002958A3 (cs) 2002-02-13
IL137909A0 (en) 2001-10-31
CA2321185C (en) 2009-04-21
GB9902290D0 (en) 1999-03-24
RU2199588C2 (ru) 2003-02-27
CA2321185A1 (en) 1999-08-26
NZ506333A (en) 2002-10-25
GB9803382D0 (en) 1998-04-15
HUP0101385A3 (en) 2003-08-28
US6287781B1 (en) 2001-09-11
WO1999042611A1 (en) 1999-08-26
EP1055002A1 (en) 2000-11-29
CN1203186C (zh) 2005-05-25
SK12212000A3 (sk) 2002-09-10
AU746149B2 (en) 2002-04-18
EP1055002B1 (en) 2008-06-11
CN1297488A (zh) 2001-05-30
AU2538399A (en) 1999-09-06
GB9903796D0 (en) 1999-04-14
ATE398187T1 (de) 2008-07-15
GB9903578D0 (en) 1999-04-07
DE69938894D1 (de) 2008-07-24
JP2003512808A (ja) 2003-04-08
HU226518B1 (en) 2009-03-30

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