BR0014017A - Método para detectar alterações em um gene através de pcr de ampla abrangência usando elementos humanos móveis - Google Patents

Método para detectar alterações em um gene através de pcr de ampla abrangência usando elementos humanos móveis

Info

Publication number
BR0014017A
BR0014017A BR0014017-1A BR0014017A BR0014017A BR 0014017 A BR0014017 A BR 0014017A BR 0014017 A BR0014017 A BR 0014017A BR 0014017 A BR0014017 A BR 0014017A
Authority
BR
Brazil
Prior art keywords
gene
change
interest
genomic dna
pcr
Prior art date
Application number
BR0014017-1A
Other languages
English (en)
Inventor
Straun Grant
Thorarinn Blondal
Original Assignee
Decode Genetics Ehf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/470,673 external-priority patent/US6225093B1/en
Application filed by Decode Genetics Ehf filed Critical Decode Genetics Ehf
Publication of BR0014017A publication Critical patent/BR0014017A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MéTODO PARA DETECTAR ALTERAçõES EM UM GENE ATRAVéS DE PCR DE AMPLA ABRANGêNCIA USANDO ELEMENTOS HUMANOS MóVEIS". Os métodos descritos são para detectar uma alteração em um gene de interesse, de modo que a deleção no gene C4A (por exemplo, para detecção de C4AQ0), através da execução da amplificação através da reação em cadeia por polimerase de ampla abrangência em uma amostra teste compreendendo o DNA genómico. Os métodos amplificam o DNA alvo compreendendo todo ou uma parte de um elemento humano móvel (por exemplo, uma inserção retroviral no intron 9 do gene C4A), usando primers construídos de modo que os produtos de PCR sejam formados apenas se a amostra teste compreender o DNA genómico apresentando a alteração no gene de interesse; alternativamente, os métodos amplificam o DNA alvo usando primers construídos de modo que os produtos de PCR sejam formados apenas se a amostra teste compreender o DNA genómico que não apresente a alteração no gene de interesse. Alternativamente, os primers são construídos de modo que os produtos de PCR tenham tamanhos dectavelmente diferentes, dependendo se a amostra teste compreende o DNA genómico que apresente a alteração no gene de interesse. Os métodos podem ser usados para identificar se um indivíduo apresenta riscos para uma doença ou para uma condição associada com a alteração no gene de interesse, como a presença de uma alteração correlacionada com os riscos para a doença.
BR0014017-1A 1999-09-07 2000-09-06 Método para detectar alterações em um gene através de pcr de ampla abrangência usando elementos humanos móveis BR0014017A (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US39124499A 1999-09-07 1999-09-07
US44353999A 1999-11-19 1999-11-19
US47159899A 1999-12-23 1999-12-23
US09/470,673 US6225093B1 (en) 1999-09-07 1999-12-23 Detection of C4A deletion by long range PCR
PCT/IB2000/001283 WO2001018245A2 (en) 1999-09-07 2000-09-06 Detection of alterations in a gene by long range pcr using human mobile elements

Publications (1)

Publication Number Publication Date
BR0014017A true BR0014017A (pt) 2002-12-31

Family

ID=27503328

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014017-1A BR0014017A (pt) 1999-09-07 2000-09-06 Método para detectar alterações em um gene através de pcr de ampla abrangência usando elementos humanos móveis

Country Status (9)

Country Link
EP (1) EP1214449A2 (pt)
JP (1) JP2003508082A (pt)
CN (1) CN1382221A (pt)
AU (1) AU6720700A (pt)
BR (1) BR0014017A (pt)
CA (1) CA2383857A1 (pt)
MX (1) MXPA02002507A (pt)
NZ (1) NZ517641A (pt)
WO (1) WO2001018245A2 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030003468A1 (en) * 2000-12-19 2003-01-02 Hospital For Special Surgery Markers for disease susceptibility and targets for therapy
TWI340168B (en) 2003-05-07 2011-04-11 Tosoh Corp Method of detecting micrometastasis
EP2397561B1 (en) 2010-06-18 2017-08-02 Progenika Biopharma, S.A. Methods for determining the presence or absence of genetic segments
DK3102722T3 (da) 2014-02-04 2020-11-16 Jumpcode Genomics Inc Genom fraktionering
US20160376663A1 (en) * 2014-02-27 2016-12-29 Igenomx International Genomics Corporation Methods for analysis of somatic mobile elements, and uses thereof
US10968536B2 (en) 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
CN113820499A (zh) * 2021-10-26 2021-12-21 深圳临研医学有限公司 用于诊断系统性红斑狼疮的蛋白标志物

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096557A (en) * 1990-07-11 1992-03-17 Genetype A.G. Internal standard for electrophoretic separations

Also Published As

Publication number Publication date
AU6720700A (en) 2001-04-10
JP2003508082A (ja) 2003-03-04
CN1382221A (zh) 2002-11-27
WO2001018245A3 (en) 2002-04-18
NZ517641A (en) 2004-02-27
WO2001018245A2 (en) 2001-03-15
EP1214449A2 (en) 2002-06-19
MXPA02002507A (es) 2004-09-10
CA2383857A1 (en) 2001-03-15

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]