WO1995033854B1 - Use of a specific marker for detection of salmonella with pcr - Google Patents
Use of a specific marker for detection of salmonella with pcrInfo
- Publication number
- WO1995033854B1 WO1995033854B1 PCT/US1995/006704 US9506704W WO9533854B1 WO 1995033854 B1 WO1995033854 B1 WO 1995033854B1 US 9506704 W US9506704 W US 9506704W WO 9533854 B1 WO9533854 B1 WO 9533854B1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sequence
- nucleic acid
- fragment
- isolated nucleic
- primer
- Prior art date
Links
- 241000607142 Salmonella Species 0.000 title claims abstract 6
- 206010039447 Salmonellosis Diseases 0.000 title claims abstract 6
- 239000003550 marker Substances 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title 1
- 241000894006 Bacteria Species 0.000 claims abstract 7
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims abstract 6
- 150000007523 nucleic acids Chemical group 0.000 claims 16
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 5
- 239000000523 sample Substances 0.000 claims 3
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 230000000295 complement Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 239000002853 nucleic acid probe Substances 0.000 claims 1
- 230000000813 microbial Effects 0.000 abstract 2
- 230000002068 genetic Effects 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Abstract
A method is provided for the selection of diagnostic genetic markers fragments and useful in the identification of bacteria at the genus, species or serotype level. The method first involves the identification of a RAPD polymorphic DNA fragment common to a particular microbial group, the identification of the most conserved regions of that fragment, and the preparation of specific primers useful for detecting the presence of a marker within the fragment whereby that set of primers is then useful in the identification of all members of the chosen microbial group. Also provided is a specific diagnostic marker for Salmonella and primers directed thereto.
Claims
AMENDED CLAIMS
[received by the International Bureau on 7 December 1995 (07.12.95); original claims 1, 3, 4 and 5 amended; remaining claims unchanged (2 pages)]
1. A method of determining whether an unknown bacterium is a member of the genus Salmonella, comprising analyzing the genomic DNA of said unknown bacterium to detect the presence of nucleic acid
Sequence ID No. 1 or a fragment thereof, or Sequence ID No. 20 or a fragment thereof whereby the presence of said nucleic acid sequence indicates said unknown bacterium is a member of said genus Salmonella . 2. The method of Claim 1 wherein said analysis comprises the steps of:
(i) performing a PCR amplification reaction on the genomic DNA of said unknown bacterium using a pair of primers comprising a first primer and a second primer wherein said first primer has a nucleic acid sequence derived from Sequence ID No. 1 and said second primer has a nucleic acid sequence derived from Sequence ID No. 20; and
(ii) detecting the presence of DNA which has been amplified by said primer pair of step (i) ; whereby the presence of amplified DNA at step (ii) indicates that said unknown bacterium is a member of the genus Salmonella .
3. The method of Claim 2 wherein at step (i) said first primer is selected from the group of fragments derived from Sequence ID No.:l consisting of Sequence ID Nos. 14 and 15, and said second primer is selected from the group of fragments derived from Sequence ID No.:20 consisting of Sequence ID Nos. 16, 17, 18, and 19.
4. The method of Claim 3 wherein at step (i) said first primer is Sequence ID No. 15 and said second primer is Sequence ID No. 19.
5. The method of Claim 1 wherein said analyzing step comprises contacting the genomic DNA of said unknown organism with a nucleic acid probe wherein said probe consists of a nucleic acid sequence which is complementary to Sequence ID Nos. 1 or 20, or a fragment thereof, and then detecting the presence of said hybridized probe, the presence of said hybridized probe indicating the presence of said sequence or fragment thereof in turn indicating that said unknown bacterium is a member of the genus Salmonella . .
6. An isolated nucleic acid fragment having Sequence ID No. 1 or a fragment thereof.
7. An isolated nucleic acid fragment having Sequence ID No. 20 or a fragment thereof. 8. An isolated nucleic acid fragment having Sequence ID No. 14.
9. An isolated nucleic acid fragment having Sequence ID No. 15.
10. An isolated nucleic acid fragment having Sequence ID No. 16.
11. An isolated nucleic acid fragment having Sequence ID No. 17.
12. An isolated nucleic acid fragment having Sequence ID No. 18. 13. An isolated nucleic acid fragment having Sequence ID No. 19.
1 . An isolated nucleic acid fragment having Sequence ID No. 21.
15. An isolated nucleic acid fragment having Sequence ID No. 22.
16. An isolated nucleic acid fragment having Sequence ID No. 4.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95922900A EP0764217B1 (en) | 1994-06-06 | 1995-06-02 | Use of a specific marker for detecting Salmonella with PCR |
| DE69528263T DE69528263T2 (en) | 1994-06-06 | 1995-06-02 | Use of a specific marker for the detection of Salmonella with PCR |
| AT95922900T ATE224453T1 (en) | 1994-06-06 | 1995-06-02 | USE OF A SPECIFIC MARKER FOR DETECTING SALMONELLA USING PCR |
| JP8501071A JP3051451B2 (en) | 1994-06-06 | 1995-06-02 | Use of specific markers for the detection of Salmonella using PCR |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/254,355 | 1994-06-06 | ||
| US08/254,355 US5660981A (en) | 1994-06-06 | 1994-06-06 | Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO1995033854A1 WO1995033854A1 (en) | 1995-12-14 |
| WO1995033854B1 true WO1995033854B1 (en) | 1996-01-04 |
Family
ID=22963980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US1995/006704 WO1995033854A1 (en) | 1994-06-06 | 1995-06-02 | Use of a specific marker for detection of salmonella with pcr |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US5660981A (en) |
| EP (1) | EP0764217B1 (en) |
| JP (1) | JP3051451B2 (en) |
| AT (1) | ATE224453T1 (en) |
| CA (1) | CA2189546A1 (en) |
| DE (1) | DE69528263T2 (en) |
| WO (1) | WO1995033854A1 (en) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5747257A (en) * | 1996-02-29 | 1998-05-05 | E. I. Du Pont De Nemours And Company | Genetic markers and methods for the detection of escherichia coli serotype-0157:H7 |
| US5922538A (en) * | 1996-11-08 | 1999-07-13 | E.I. Du Pont De Nemours And Company | Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp |
| US6821770B1 (en) * | 1999-05-03 | 2004-11-23 | Gen-Probe Incorporated | Polynucleotide matrix-based method of identifying microorganisms |
| US20020120408A1 (en) * | 2000-09-06 | 2002-08-29 | Kreiswirth Barry N. | System and method for tracking and controlling infections |
| US7349808B1 (en) * | 2000-09-06 | 2008-03-25 | Egenomics, Inc. | System and method for tracking and controlling infections |
| NZ530343A (en) * | 2001-06-22 | 2007-01-26 | Marshfield Clinic | Methods and oligonucleotides for the detection of E. coli 0157:H7 |
| US6696254B2 (en) * | 2001-11-21 | 2004-02-24 | Kimberly-Clark Worldwide, Inc. | Detection and identification of enteric bacteria |
| US7309589B2 (en) | 2004-08-20 | 2007-12-18 | Vironix Llc | Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing |
| KR100671501B1 (en) * | 2005-02-28 | 2007-01-19 | 삼성에버랜드 주식회사 | Primer for detecting food poisoning and method for rapid detection of food born pathogene |
| US20060246463A1 (en) * | 2005-04-20 | 2006-11-02 | Vevea Dirk N | Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes |
| US20060240442A1 (en) * | 2005-04-20 | 2006-10-26 | Vevea Dirk N | Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes |
| WO2007076501A2 (en) * | 2005-12-23 | 2007-07-05 | The Burnham Institute For Medical Research | Endometriosis cell targeting peptide and uses thereof |
| AU2012335983B2 (en) * | 2011-11-09 | 2018-05-31 | Qualicon Diagnostics, Llc | Sequences and their use for detection of Salmonella |
| TWI464269B (en) * | 2012-10-16 | 2014-12-11 | Univ Hungkuang | Nucleic acid molecule, diagnostic kit, biochip and method for the detection of salmonella strains |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4717653A (en) * | 1981-09-25 | 1988-01-05 | Webster John A Jr | Method for identifying and characterizing organisms |
| US5087558A (en) * | 1981-09-25 | 1992-02-11 | Webster John A Jr | Method for identifying and characterizing organisms |
| US4689295A (en) * | 1983-01-20 | 1987-08-25 | Integrated Genetics, Inc. | Test for Salmonella |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| EP0389564B1 (en) * | 1987-12-01 | 1996-01-24 | Amoco Corporation | Detection of salmonella |
| NL8900670A (en) * | 1989-03-17 | 1990-10-16 | Stichting Tech Wetenschapp | POLYNUCLEOTIDE PROBE, METHOD AND KIT FOR IDENTIFYING AND DETECTING GRAM-NEGATIVE BACTERIA. |
| IE81145B1 (en) * | 1989-04-20 | 2000-05-03 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
| WO1990015157A1 (en) * | 1989-05-31 | 1990-12-13 | Gene-Trak Systems | Universal eubacteria nucleic acid probes and methods |
| US5126239A (en) * | 1990-03-14 | 1992-06-30 | E. I. Du Pont De Nemours And Company | Process for detecting polymorphisms on the basis of nucleotide differences |
| JPH06500471A (en) * | 1990-08-24 | 1994-01-20 | ザ・ユニバーシティ・オブ・テネシー・リサーチ・コーポレーション | DNA amplification fingerprinting |
| WO1992007095A1 (en) * | 1990-10-15 | 1992-04-30 | Stratagene | Arbitrarily primed polymerase chain reaction method for fingerprinting genomes |
| US5071862A (en) * | 1990-10-26 | 1991-12-10 | Uniroyal Chemical Company, Inc. | 2-(3-oxypyridinyl)-1,3-dioxolane and dioxane compounds, compositions containing same and process for controlling fungi using same |
| JPH05505311A (en) * | 1990-11-06 | 1993-08-12 | ザ ルブリゾル コーポレーション | Compositions and methods for analyzing genomic variation |
| CA2055302A1 (en) * | 1990-11-15 | 1992-05-16 | Roger G. L. Wheatcroft | Specific microbial strain detection method |
| US5437975A (en) * | 1991-02-25 | 1995-08-01 | California Institute Of Biological Research | Consensus sequence primed polymerase chain reaction method for fingerprinting genomes |
| EP0517361A1 (en) * | 1991-05-30 | 1992-12-09 | Amoco Corporation | A method for detecting and identifying pathogenic organisms using target sequences as detectors |
| EP0517154A1 (en) * | 1991-06-06 | 1992-12-09 | F. Hoffmann-La Roche Ag | Method for detecting giardia |
| WO1993004202A1 (en) * | 1991-08-22 | 1993-03-04 | Washington University | Polynucleotide probes for salmonella |
| CA2077135A1 (en) * | 1991-08-30 | 1993-03-01 | Joh-E Ikeda | A method of dna amplification |
| US5340728A (en) * | 1992-12-09 | 1994-08-23 | E. I. Du Pont De Nemours And Company | Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction |
-
1994
- 1994-06-06 US US08/254,355 patent/US5660981A/en not_active Expired - Lifetime
-
1995
- 1995-06-02 DE DE69528263T patent/DE69528263T2/en not_active Expired - Lifetime
- 1995-06-02 CA CA002189546A patent/CA2189546A1/en not_active Abandoned
- 1995-06-02 JP JP8501071A patent/JP3051451B2/en not_active Expired - Lifetime
- 1995-06-02 AT AT95922900T patent/ATE224453T1/en not_active IP Right Cessation
- 1995-06-02 WO PCT/US1995/006704 patent/WO1995033854A1/en active IP Right Grant
- 1995-06-02 EP EP95922900A patent/EP0764217B1/en not_active Expired - Lifetime
-
1996
- 1996-01-16 US US08/586,120 patent/US5656740A/en not_active Expired - Lifetime
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