WO2000065093A3 - In situ hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms - Google Patents

In situ hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms Download PDF

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Publication number
WO2000065093A3
WO2000065093A3 PCT/US2000/010874 US0010874W WO0065093A3 WO 2000065093 A3 WO2000065093 A3 WO 2000065093A3 US 0010874 W US0010874 W US 0010874W WO 0065093 A3 WO0065093 A3 WO 0065093A3
Authority
WO
WIPO (PCT)
Prior art keywords
situ hybridization
occurrence
reducing
false positives
probes
Prior art date
Application number
PCT/US2000/010874
Other languages
French (fr)
Other versions
WO2000065093A9 (en
WO2000065093A2 (en
Inventor
Curtis T Thompson
Joseph A Spidle
Original Assignee
Science & Technology Corp
Univ New Mexico
Curtis T Thompson
Joseph A Spidle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Science & Technology Corp, Univ New Mexico, Curtis T Thompson, Joseph A Spidle filed Critical Science & Technology Corp
Priority to AU46563/00A priority Critical patent/AU4656300A/en
Publication of WO2000065093A2 publication Critical patent/WO2000065093A2/en
Publication of WO2000065093A3 publication Critical patent/WO2000065093A3/en
Publication of WO2000065093A9 publication Critical patent/WO2000065093A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates

Abstract

This invention provides novel methods for detecting microorganisms in biological samples. The methods utilize in situ hybridization techniques. In one preferred embodiment, the methods performing an in situ hybridization using two or more probes specific to the same target microorganism where the probes are each labeled with different and distinguishable label or are themselves different and distinguishable. When the probes co-localize to the same feature they are scored as positive for the target microorganism corresponding to those eprobes. Where only a single probe localizes to the target the result is scored as negative and/or, optionally subject to re-testing or other further analysis.
PCT/US2000/010874 1999-04-22 2000-04-21 In situ hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms WO2000065093A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU46563/00A AU4656300A (en) 1999-04-22 2000-04-21 (in situ) hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US13086599P 1999-04-22 1999-04-22
US60/130,865 1999-04-22
US55900300A 2000-04-21 2000-04-21
US09/559,003 2000-04-21

Publications (3)

Publication Number Publication Date
WO2000065093A2 WO2000065093A2 (en) 2000-11-02
WO2000065093A3 true WO2000065093A3 (en) 2001-05-03
WO2000065093A9 WO2000065093A9 (en) 2001-08-16

Family

ID=26828900

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/010874 WO2000065093A2 (en) 1999-04-22 2000-04-21 In situ hybridization methods for reducing the occurrence of false positives and for targeting multiple microorganisms

Country Status (1)

Country Link
WO (1) WO2000065093A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10061655A1 (en) * 2000-12-11 2002-06-20 Vermicon Ag In situ hybridization arrangement for the specific detection of microorganisms
JP2005515756A (en) * 2001-06-19 2005-06-02 バーミコン　アクチェンゲゼルシャフト Method for specific and rapid detection of related bacteria in drinking water
DE10129410A1 (en) * 2001-06-19 2003-01-02 Vermicon Ag Process for the specific rapid detection of beer-damaging bacteria
NO321928B1 (en) * 2004-12-23 2006-07-24 Oystein Ljungmann Method and system for using treatment liquids in an apparatus for staining tissue samples on slides
DE102007021387A1 (en) 2007-05-04 2008-11-06 Eads Deutschland Gmbh Detection device for the detection of biological microparticles such as bacteria, viruses, spores, pollen or biological toxins, and detection methods
US9404160B2 (en) * 2009-12-22 2016-08-02 Becton, Dickinson And Company Methods for the detection of microorganisms
EP3554257B1 (en) 2016-12-15 2023-07-12 Société des Produits Nestlé S.A. Compositions and methods that modulate white blood cells or neutrophils in a companion animal
EP3865592A3 (en) * 2020-02-14 2021-08-25 Testo bioAnalytics GmbH Oligonucleotide probe for the specific detection of microorganisms, corresponding method and use
EP3865589A3 (en) * 2020-02-14 2021-12-22 Testo bioAnalytics GmbH Method for detecting microorganisms, corresponding use and sample carrier

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014442A1 (en) * 1989-05-24 1990-11-29 Gene-Trak Systems Nucleic acid probes for the detection of neisseria gonorrhoeae
WO1992005277A1 (en) * 1990-09-26 1992-04-02 William Scott Davidson Test to determine an organism's species and/or population identity by direct nucleotide sequence analysis of defined segments of its genome
EP0497464A1 (en) * 1991-01-31 1992-08-05 Amoco Corporation Rapid microbial diagnostics by in situ hybridization in aqueous suspension
WO1994002645A1 (en) * 1992-07-17 1994-02-03 Aprogenex, Inc. Rapid detection of biopolymers in stained specimens
WO1994028174A1 (en) * 1993-05-24 1994-12-08 Amoco Corporation Nucleic acid probes for bacteria of the genus legionella
WO1996036734A1 (en) * 1995-05-18 1996-11-21 Abbott Laboratories Polymeric peptide probes and uses thereof
EP0745690A2 (en) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide Probes, Assays and Kits
US5639612A (en) * 1992-07-28 1997-06-17 Hitachi Chemical Company, Ltd. Method for detecting polynucleotides with immobilized polynucleotide probes identified based on Tm
WO1998015648A1 (en) * 1996-10-04 1998-04-16 Dako A/S Novel probes for the detection of mycobacteria
EP0878552A1 (en) * 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molecular detection of chromosome aberrations
WO1999010533A1 (en) * 1997-08-26 1999-03-04 Microscreen B.V. Rapid detection and identification of micro-organisms

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014442A1 (en) * 1989-05-24 1990-11-29 Gene-Trak Systems Nucleic acid probes for the detection of neisseria gonorrhoeae
WO1992005277A1 (en) * 1990-09-26 1992-04-02 William Scott Davidson Test to determine an organism's species and/or population identity by direct nucleotide sequence analysis of defined segments of its genome
EP0497464A1 (en) * 1991-01-31 1992-08-05 Amoco Corporation Rapid microbial diagnostics by in situ hybridization in aqueous suspension
WO1994002645A1 (en) * 1992-07-17 1994-02-03 Aprogenex, Inc. Rapid detection of biopolymers in stained specimens
US5639612A (en) * 1992-07-28 1997-06-17 Hitachi Chemical Company, Ltd. Method for detecting polynucleotides with immobilized polynucleotide probes identified based on Tm
WO1994028174A1 (en) * 1993-05-24 1994-12-08 Amoco Corporation Nucleic acid probes for bacteria of the genus legionella
EP0745690A2 (en) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide Probes, Assays and Kits
WO1996036734A1 (en) * 1995-05-18 1996-11-21 Abbott Laboratories Polymeric peptide probes and uses thereof
WO1998015648A1 (en) * 1996-10-04 1998-04-16 Dako A/S Novel probes for the detection of mycobacteria
EP0878552A1 (en) * 1997-05-13 1998-11-18 Erasmus Universiteit Rotterdam Molecular detection of chromosome aberrations
WO1999010533A1 (en) * 1997-08-26 1999-03-04 Microscreen B.V. Rapid detection and identification of micro-organisms

Also Published As

Publication number Publication date
WO2000065093A9 (en) 2001-08-16
WO2000065093A2 (en) 2000-11-02

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