DE69702649D1 - Fluoreszenz-nachweistest für homogene pcr-hybridisierungssysteme - Google Patents

Fluoreszenz-nachweistest für homogene pcr-hybridisierungssysteme

Info

Publication number
DE69702649D1
DE69702649D1 DE69702649T DE69702649T DE69702649D1 DE 69702649 D1 DE69702649 D1 DE 69702649D1 DE 69702649 T DE69702649 T DE 69702649T DE 69702649 T DE69702649 T DE 69702649T DE 69702649 D1 DE69702649 D1 DE 69702649D1
Authority
DE
Germany
Prior art keywords
oligonucleotide
nucleic acid
pcr
probe
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69702649T
Other languages
English (en)
Other versions
DE69702649T2 (de
Inventor
Cesare L Di
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DE69702649D1 publication Critical patent/DE69702649D1/de
Publication of DE69702649T2 publication Critical patent/DE69702649T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69702649T 1996-02-05 1997-01-31 Fluoreszenz-nachweistest für homogene pcr-hybridisierungssysteme Expired - Lifetime DE69702649T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/596,560 US5716784A (en) 1996-02-05 1996-02-05 Fluorescence detection assay for homogeneous PCR hybridization systems
PCT/US1997/001755 WO1997029210A2 (en) 1996-02-05 1997-01-31 Fluorescence detection assay for homogeneous pcr hybridization systems

Publications (2)

Publication Number Publication Date
DE69702649D1 true DE69702649D1 (de) 2000-08-31
DE69702649T2 DE69702649T2 (de) 2001-04-05

Family

ID=24387797

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69702649T Expired - Lifetime DE69702649T2 (de) 1996-02-05 1997-01-31 Fluoreszenz-nachweistest für homogene pcr-hybridisierungssysteme

Country Status (8)

Country Link
US (1) US5716784A (de)
EP (1) EP0870063B1 (de)
JP (1) JP3415627B2 (de)
AT (1) ATE195004T1 (de)
AU (1) AU700521B2 (de)
CA (1) CA2243759C (de)
DE (1) DE69702649T2 (de)
WO (1) WO1997029210A2 (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5641633A (en) * 1995-11-15 1997-06-24 Becton, Dickinson And Company Fluorescence polarization detection of nucleic acids
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US7803528B1 (en) * 1997-02-28 2010-09-28 Quest Diagnostics Incorporated Fluorescence energy transfer by competitive hybridization
EP1666609B1 (de) * 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluoreszens-Energie-Transfer durch kompetitive Hybridisierung
US6294326B1 (en) * 1997-11-07 2001-09-25 Abbott Laboratories Analyte detection process using dual labeled probes
DE69838210T2 (de) * 1997-12-15 2008-05-15 Csl Behring Gmbh Markierter Primer, geeignet für die Detektion von Nukleinsäuren
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
WO1999042615A1 (en) * 1998-02-18 1999-08-26 Dade Behring Inc. Methods for determining amounts of nucleic acids
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
GB9815799D0 (en) 1998-07-21 1998-09-16 Pharmagene Lab Limited Quantitative analysis of gene expression
GB2342651B (en) 1998-09-18 2001-10-17 Massachusetts Inst Technology Biological applications of semiconductor nanocrystals
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
US6184347B1 (en) 1998-11-19 2001-02-06 Agilent Technologies Inc. Minimization of blooming in high-density arrays by using reactive wash reagents
EP1013775A1 (de) * 1998-12-21 2000-06-28 LUTZ, Hans Quantitative Polymerase-Kettenreaktion mittels fluorogenen Echtzeiterfassungssystem
WO2000043942A2 (en) * 1999-01-25 2000-07-27 Combimatrix Corporation Method for selecting oligonucleotides having low cross-hybridization
US6355246B1 (en) 1999-06-10 2002-03-12 Board Of Trustees Of Michigan State University Feline calicivirus isolated from cat urine and vaccines thereof
WO2001013079A1 (en) * 1999-08-18 2001-02-22 Swinburne University Method and apparatus for the resolution of beams of electromagnetic radiation
US6355435B1 (en) 1999-09-10 2002-03-12 Board Of Trustees Of Michigan State University Methods for detecting and enumerating Campylobacter jejuni in environmental samples and for identifying antibiotic-resistant strains
US7125665B2 (en) * 2000-01-26 2006-10-24 Albert M. Bobst Detection of nucleic acid target sequences by electron paramagnetic resonance spectroscopy
FI20000333A0 (fi) * 2000-02-16 2000-02-16 Jussi Nurmi Homogeeninen menetelmä polynukleotidin havaitsemiseksi
ATE409238T1 (de) * 2000-05-12 2008-10-15 Caliper Life Sciences Inc Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
EP2351853A1 (de) 2000-06-06 2011-08-03 Life Technologies Corporation Methode und Vorrichtungen für multiplex Amplifizierungsreaktionen
ES2267803T3 (es) 2000-08-11 2007-03-16 University Of Utah Research Foundation Sondas de oligonucleotidos marcados individuales.
CN1348096A (zh) * 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
US6867021B2 (en) 2001-02-20 2005-03-15 Board Of Trustees Of Michigan State University Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
JP4601427B2 (ja) * 2002-09-18 2010-12-22 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア 細胞上および生物学的混合物中の抗原の検出のための組成物、方法およびキット
JP2004113108A (ja) * 2002-09-26 2004-04-15 Aisin Seiki Co Ltd Ｄｎａ回収法
WO2004051218A2 (en) 2002-12-04 2004-06-17 Applera Corporation Multiplex amplification of polynucleotides
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
CA2548205C (en) 2003-12-03 2017-06-27 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
US7122799B2 (en) * 2003-12-18 2006-10-17 Palo Alto Research Center Incorporated LED or laser enabled real-time PCR system and spectrophotometer
US7582430B2 (en) * 2004-01-20 2009-09-01 United States Of America As Represented By The Secretary Of The Army Immunoliposome-nucleic acid amplification (ILNAA) assay
KR100906749B1 (ko) * 2004-03-25 2009-07-09 (주)바이오니아 인터컬레이팅 형광염료가 표지된 프로브를 이용한 핵산 증폭 측정 방법
US7468249B2 (en) * 2004-05-05 2008-12-23 Biocept, Inc. Detection of chromosomal disorders
KR101424812B1 (ko) * 2004-10-18 2014-08-07 브랜데이스 유니버시티 핵산 증폭을 위한 프라이머, 프로브 및 방법
EP1788095B1 (de) 2005-11-18 2011-04-13 Eppendorf Array Technologies SA Reaktionsgefäss zur Real-time PCR umfassend Oligonukleotidproben wobei die Amplifikationsprodukte durch Hybridisierung und ohne Öffnen des Reaktionsgefässes gemessen werden
EP2302072B1 (de) 2004-11-18 2012-03-14 Eppendorf Array Technologies S.A. Echtzeit-PCR von Nukleinsäuren auf einem Mikroarray
US20070020644A1 (en) * 2005-01-26 2007-01-25 Kolykhalov Alexander A Method for detection and characterization of short nucleic acids
AU2006273753B2 (en) * 2005-07-28 2010-06-24 Zoetis Services Llc Methods of vaccine administration, new feline caliciviruses, and treatments for immunizing animals against feline parvovirus and feline herpes virus
WO2007045890A1 (en) * 2005-10-21 2007-04-26 Primerdesign Ltd Probe
EP2025762A3 (de) 2006-01-17 2009-09-30 Health Research Inc. Heteroduplex-Trackingassay
EP2019687B1 (de) 2006-03-29 2014-03-19 Merial Limited Impfstoff gegen streptokokken
WO2008074023A2 (en) 2006-12-13 2008-06-19 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
WO2008093002A2 (en) * 2007-02-01 2008-08-07 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
EP2107125A1 (de) 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Echtzeit-PCR von Targets auf einem Mikroarray
EP2385371B1 (de) 2008-09-22 2014-10-22 Oregon Health and Science University Verfahren zum Erkennen einer Infektion mit dem Mycobakterium tuberculosis
EP2352847B1 (de) 2008-11-10 2014-01-08 The United States of America, as represented by The Secretary, Department of Health and Human Services Gensignatur zur vorhersage der prognose von patienten mit soliden tumoren
WO2010093872A2 (en) 2009-02-13 2010-08-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Molecular-based method of cancer diagnosis and prognosis
US20120004133A1 (en) 2009-02-20 2012-01-05 The Johns Hopkins University Method for the diagnosis of age-associated vascular disorders
WO2010138908A1 (en) 2009-05-29 2010-12-02 Ventana Medical Systems, Inc. Igfir gene copy number as a prognostic marker in a non-small cell lung cancer
US20120141472A1 (en) 2009-05-29 2012-06-07 Shalini Singh Methods of scoring gene copy number in a biological sample using in situ hybridization
WO2011063283A2 (en) 2009-11-20 2011-05-26 Oregon Health & Science University Methods for detecting a mycobacterium tuberculosis infection
CA2793660C (en) 2010-03-19 2020-12-22 The Translational Genomics Research Institute Methods, kits and compositions for detection of mrsa
EA201391074A1 (ru) 2011-01-25 2013-12-30 Олмак Дайэгностикс Лимитед Профили экспрессии генов рака толстой кишки и способы применения
WO2013006684A1 (en) 2011-07-05 2013-01-10 The Gov. Of The U.S.A. As Represented By The Secretary Of The Dept. Of Health And Human Services. Hiv-1 genotyping assay for global surveillance of hiv-1 drug resistance
CN108192952A (zh) 2011-09-29 2018-06-22 露美内克丝公司 水解探针
CN108103145A (zh) * 2011-10-31 2018-06-01 荣研化学株式会社 靶核酸的检测方法
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
JP6366580B2 (ja) 2012-06-22 2018-08-01 エイチティージー モレキュラー ダイアグノスティクス， インコーポレイテッド メラニン細胞性病変における分子悪性度
US10246739B2 (en) 2013-03-15 2019-04-02 Georgetown University Exonuclease cycling assay
WO2014145347A1 (en) 2013-03-15 2014-09-18 The Translational Genomics Research Institute Methods for the diagnosis of amyotrophic lateral sclerosis
EP3521452B1 (de) 2013-06-19 2021-08-04 Luminex Corporation Multiplexierter echtzeithydrolysesondentest
DE102013213279B4 (de) 2013-07-06 2024-03-28 Ist Innuscreen Gmbh Universelles Verfahren zur Detektion von diversen Analyten in Form von Nukleinsäuresequenzen
WO2015021460A1 (en) 2013-08-09 2015-02-12 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
US10584387B2 (en) 2013-10-09 2020-03-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Detection of hepatitis delta virus (HDV) for the diagnosis and treatment of Sjögren's syndrome and lymphoma
WO2015073709A2 (en) 2013-11-14 2015-05-21 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Detection of atherosclerotic cardiovascular disease risk and heart failure risk
US10398772B2 (en) 2014-01-08 2019-09-03 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Ras pathways as markers of protection against HIV and methods to improve vaccine efficacy
US9982291B2 (en) 2014-08-11 2018-05-29 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2017070244A1 (en) 2015-10-20 2017-04-27 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biodosimetry analysis
CN108884495B (zh) 2016-02-08 2023-08-22 美国政府(由卫生和人类服务部的部长所代表) 基因标记预测肝细胞癌对经导管动脉化疗栓塞(tace)的应答
WO2017189976A1 (en) 2016-04-29 2017-11-02 Board Of Regents, The University Of Texas System Targeted measure of transcriptional activity related to hormone receptors
CN109652521A (zh) * 2018-12-28 2019-04-19 黄劭 一种含有人工标签的杂交探针
CA3217862A1 (en) 2021-05-05 2022-11-10 Radius Pharmaceuticals, Inc. Animal model having homologous recombination of mouse pth1 receptor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0232967B1 (de) * 1986-01-10 1993-04-28 Amoco Corporation Kompetitiver homogener Test
CA2033692A1 (en) * 1990-01-25 1991-07-26 Wilhelm Bannwarth Energy transfer systems
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase

Also Published As

Publication number Publication date
CA2243759C (en) 2002-05-21
DE69702649T2 (de) 2001-04-05
EP0870063A2 (de) 1998-10-14
WO1997029210A3 (en) 1997-10-09
EP0870063B1 (de) 2000-07-26
WO1997029210A2 (en) 1997-08-14
AU700521B2 (en) 1999-01-07
CA2243759A1 (en) 1997-08-14
ATE195004T1 (de) 2000-08-15
US5716784A (en) 1998-02-10
JP3415627B2 (ja) 2003-06-09
JPH11506020A (ja) 1999-06-02
AU1856697A (en) 1997-08-28

Similar Documents

Publication Publication Date Title
DE69702649D1 (de) Fluoreszenz-nachweistest für homogene pcr-hybridisierungssysteme
JP7063886B2 (ja) マルチプレックスリアルタイムｐｃｒを実施する方法
HUP0101385A2 (hu) Eljárás nukleinsavak detektálására PCR-módszerrel
US7081339B2 (en) Methods for variation detection
KR100641595B1 (ko) 핵산의 형광측정 검출 시스템
CN106244703B (zh) 基于粘性末端-介导的链置换反应结合聚合切刻等温扩增技术检测udg活性的方法
EP1977002A1 (de) Verfahren zur quantifizierung von nukleinsäuren, insbesondere mit bisulfit behandelter dna
EP0374665A3 (de) Prüfung von Sequenzen mittels amplifizierter Gene
CA2692633A1 (en) Method for the simultaneous detection of multiple nucleic acid sequences in a sample
CN102112632A (zh) 用于pcr分析的检测算法
CN107760764A (zh) 一种基于引物荧光和淬灭标记的靶核酸检测方法和试剂盒
IE910559A1 (en) Quantitative determination of a dna polymerase and a test¹kit useful in same
EP1108789A2 (de) Quantifizierung der Expression der hTERT-mRNS
RU2002123871A (ru) Способ детекции и количественного определения аденовирусов
CN100487432C (zh) 采用分子信标对核酸信号进行恒温放大与检测的方法
CN102212615A (zh) 基于耐高温RNase HII的单核苷酸多态性检测方法
US20220298555A1 (en) Detection of target nucleic acid by solid-phase molography
ATE541947T1 (de) Diagnosetests auf basis einer nukleinsäuresonde für prokaryotische und eukaryotische organismen
EP1390533B1 (de) Analytische methode und testkit
AU2001235856B2 (en) Method for analysing the length of a nucleic acid molecule
McChlery et al. The use of hydrolysis and hairpin probes in real-time PCR
CA2381037A1 (en) Amplification method for detection of target nucleic acids involving fluorescence energy transfer
BR0014017A (pt) Método para detectar alterações em um gene através de pcr de ampla abrangência usando elementos humanos móveis
Saunders Quantitative real-time PCR
CN104561248A (zh) 用于检测靶核酸的引物和其应用

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

Free format text: PFANDRECHT AUFGEHOBEN