ZA200109333B - Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms. - Google Patents

Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms.

Info

Publication number
ZA200109333B
ZA200109333B ZA200109333A ZA200109333A ZA200109333B ZA 200109333 B ZA200109333 B ZA 200109333B ZA 200109333 A ZA200109333 A ZA 200109333A ZA 200109333 A ZA200109333 A ZA 200109333A ZA 200109333 B ZA200109333 B ZA 200109333B
Authority
ZA
South Africa
Prior art keywords
homology
organisms
prokaryotic
nucleic acid
tmrna
Prior art date
Application number
ZA200109333A
Inventor
Thomas Gerard Barry
Terence James Smith
Original Assignee
Entpr Ie Trd As Bioresearch Ie
Nat Univ Ireland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Entpr Ie Trd As Bioresearch Ie, Nat Univ Ireland filed Critical Entpr Ie Trd As Bioresearch Ie
Publication of ZA200109333B publication Critical patent/ZA200109333B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P33/00—Antiparasitic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Use of the ssrA gene or tmRNA, an RNA transcript of the ssrA gene, or fragments thereof as target regions in a nucleic acid probe assay for the detection and identification of prokaryotic and/or eukaryotic organisms is described. Nucleotide sequence alignment of tmRNA sequences from various organisms can be used to identify regions of homology and non-homology within the sequences which in turn can be used to design both genus specific and species specific oligonucleotide probes. These newly identified regions of homology and non-homology provide the basis of identifying and detecting organisms at the molecular level. Oligonucleotide probes identified in this way can be used to detect tmRNA in samples thereby giving an indication of the viability of non- viral organisms present in various sample types.
ZA200109333A 1999-05-14 2001-11-13 Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms. ZA200109333B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IE9900043 1999-05-14

Publications (1)

Publication Number Publication Date
ZA200109333B true ZA200109333B (en) 2003-01-29

Family

ID=11042522

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200109333A ZA200109333B (en) 1999-05-14 2001-11-13 Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms.

Country Status (12)

Country Link
US (4) US7972777B1 (en)
EP (5) EP1179090B1 (en)
JP (3) JP2003526334A (en)
CN (2) CN1350592B (en)
AT (3) ATE541948T1 (en)
AU (1) AU4427100A (en)
CA (1) CA2373290C (en)
DE (1) DE60044294D1 (en)
ES (3) ES2380577T3 (en)
MX (1) MXPA01011635A (en)
WO (1) WO2000070086A1 (en)
ZA (1) ZA200109333B (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2613159A1 (en) * 2004-07-06 2006-01-12 Aes Chemunex S.A. Polynucleotides for the detection of listeria species
US7662562B2 (en) * 2004-08-10 2010-02-16 Becton, Dickinson And Company Method for rapid identification of microorganisms
WO2009122388A1 (en) * 2008-03-31 2009-10-08 National University Of Ireland, Galway A method for the detection of group b streptococcus (gbs) (streptococcus agalactiae) in mammals
WO2010115912A2 (en) * 2009-04-09 2010-10-14 National Unversity Of Ireland, Galway Nucleic acid sequences based on the bacterial ssra gene and its tmrna transcript for the molecular detection of s.aureus
WO2010142702A1 (en) * 2009-06-08 2010-12-16 National University Of Ireland, Galway A rapid real-time nucleic acid based method for the detection of salmonella species
EP2558596B1 (en) * 2010-04-16 2018-03-14 The Government Of The United States Of America As Reresented By The Secretary Of The Department Of Health & Human Services Real time pcr assay for detection of bacterial respiratory pathogens
US9394574B2 (en) 2012-06-12 2016-07-19 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Methods for detecting Legionella nucleic acids in a sample
CA2916352C (en) * 2013-07-03 2020-09-08 Qvella Corporation Methods of targeted antibiotic susceptibility testing
GB201508860D0 (en) 2015-05-22 2015-07-01 Nat Univ Ireland Diagnostic method
CN107012214A (en) * 2017-03-28 2017-08-04 西藏自治区农牧科学院农业资源与环境研究所 A kind of method of mycoplasma in quick detection milk frozen cattle semens
CN114302968A (en) * 2019-08-29 2022-04-08 美国西门子医学诊断股份有限公司 Reagents and methods for detecting AAV shedding

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4038804A1 (en) * 1990-10-09 1992-04-16 Boehringer Mannheim Gmbh METHOD FOR GENUS AND / AND SPECIES-SPECIFIC DETECTION OF BACTERIA IN A SAMPLING LIQUID
IE81145B1 (en) * 1989-04-20 2000-05-03 Thomas Gerard Barry Generation of specific probes for target nucleotide sequences
GB9020621D0 (en) * 1990-09-21 1990-10-31 Imp Cancer Res Tech Identification of organisms
US5550040A (en) * 1993-06-23 1996-08-27 Hoffman-La Roche Inc. Method, reagents and kits for the detection of Neisseria gonorrhoeae
CA2265895A1 (en) * 1996-09-05 1998-03-12 The Government Of The United States Of America, Represented By The Secre Tary, Department Of Health And Human Services Nucleic acid assay for the detection and differentiation of three chlamydia species
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
WO1998048008A1 (en) * 1997-04-23 1998-10-29 Plueckthun Andreas Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
US6096501A (en) * 1997-11-04 2000-08-01 Becton Dickinson And Company Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis crytpic plasmid
US6004754A (en) * 1998-01-21 1999-12-21 Becton Dickinson And Company DNA sequence, related probes and primers for the detection of Streptococcus agalactiae
US7115366B1 (en) * 1999-04-07 2006-10-03 University Of Utah Research Foundation Eubacterial tmRNA sequences and uses thereof
WO2000059918A1 (en) * 1999-04-07 2000-10-12 University Of Utah Research Foundation EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF
JP4527621B2 (en) * 2005-07-19 2010-08-18 株式会社小糸製作所 Vehicle lighting
US20090246764A1 (en) * 2008-03-31 2009-10-01 National University Of Ireland Galway Method for the detection of group B Streptococcus (GBS) (Streptococcus agalactiae) in mammals

Also Published As

Publication number Publication date
US7972777B1 (en) 2011-07-05
ES2380567T3 (en) 2012-05-16
MXPA01011635A (en) 2003-09-10
AU4427100A (en) 2000-12-05
CN1350592B (en) 2014-02-19
EP2230317A1 (en) 2010-09-22
JP5902749B2 (en) 2016-04-13
EP2236629A1 (en) 2010-10-06
DE60044294D1 (en) 2010-06-10
ES2380577T3 (en) 2012-05-16
ATE541947T1 (en) 2012-02-15
EP2360276A1 (en) 2011-08-24
US20120258453A1 (en) 2012-10-11
EP2236629B1 (en) 2012-01-18
US20110111398A1 (en) 2011-05-12
ATE541948T1 (en) 2012-02-15
US8946127B2 (en) 2015-02-03
CN1350592A (en) 2002-05-22
CN103255206A (en) 2013-08-21
EP1179090B1 (en) 2010-04-28
ES2345243T3 (en) 2010-09-20
CA2373290C (en) 2012-04-17
ATE466101T1 (en) 2010-05-15
WO2000070086A9 (en) 2001-04-19
EP2365097A1 (en) 2011-09-14
EP2230317B1 (en) 2012-01-18
CA2373290A1 (en) 2000-11-23
EP1179090A1 (en) 2002-02-13
US20130296179A1 (en) 2013-11-07
JP2014195456A (en) 2014-10-16
WO2000070086A1 (en) 2000-11-23
JP2003526334A (en) 2003-09-09
JP2011062202A (en) 2011-03-31

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