ZA200109333B - Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms. - Google Patents
Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms.Info
- Publication number
- ZA200109333B ZA200109333B ZA200109333A ZA200109333A ZA200109333B ZA 200109333 B ZA200109333 B ZA 200109333B ZA 200109333 A ZA200109333 A ZA 200109333A ZA 200109333 A ZA200109333 A ZA 200109333A ZA 200109333 B ZA200109333 B ZA 200109333B
- Authority
- ZA
- South Africa
- Prior art keywords
- homology
- organisms
- prokaryotic
- nucleic acid
- tmrna
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Use of the ssrA gene or tmRNA, an RNA transcript of the ssrA gene, or fragments thereof as target regions in a nucleic acid probe assay for the detection and identification of prokaryotic and/or eukaryotic organisms is described. Nucleotide sequence alignment of tmRNA sequences from various organisms can be used to identify regions of homology and non-homology within the sequences which in turn can be used to design both genus specific and species specific oligonucleotide probes. These newly identified regions of homology and non-homology provide the basis of identifying and detecting organisms at the molecular level. Oligonucleotide probes identified in this way can be used to detect tmRNA in samples thereby giving an indication of the viability of non- viral organisms present in various sample types.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IE9900043 | 1999-05-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
ZA200109333B true ZA200109333B (en) | 2003-01-29 |
Family
ID=11042522
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ZA200109333A ZA200109333B (en) | 1999-05-14 | 2001-11-13 | Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms. |
Country Status (12)
Country | Link |
---|---|
US (4) | US7972777B1 (en) |
EP (5) | EP1179090B1 (en) |
JP (3) | JP2003526334A (en) |
CN (2) | CN1350592B (en) |
AT (3) | ATE541948T1 (en) |
AU (1) | AU4427100A (en) |
CA (1) | CA2373290C (en) |
DE (1) | DE60044294D1 (en) |
ES (3) | ES2380577T3 (en) |
MX (1) | MXPA01011635A (en) |
WO (1) | WO2000070086A1 (en) |
ZA (1) | ZA200109333B (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2613159A1 (en) * | 2004-07-06 | 2006-01-12 | Aes Chemunex S.A. | Polynucleotides for the detection of listeria species |
US7662562B2 (en) * | 2004-08-10 | 2010-02-16 | Becton, Dickinson And Company | Method for rapid identification of microorganisms |
WO2009122388A1 (en) * | 2008-03-31 | 2009-10-08 | National University Of Ireland, Galway | A method for the detection of group b streptococcus (gbs) (streptococcus agalactiae) in mammals |
WO2010115912A2 (en) * | 2009-04-09 | 2010-10-14 | National Unversity Of Ireland, Galway | Nucleic acid sequences based on the bacterial ssra gene and its tmrna transcript for the molecular detection of s.aureus |
WO2010142702A1 (en) * | 2009-06-08 | 2010-12-16 | National University Of Ireland, Galway | A rapid real-time nucleic acid based method for the detection of salmonella species |
EP2558596B1 (en) * | 2010-04-16 | 2018-03-14 | The Government Of The United States Of America As Reresented By The Secretary Of The Department Of Health & Human Services | Real time pcr assay for detection of bacterial respiratory pathogens |
US9394574B2 (en) | 2012-06-12 | 2016-07-19 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Methods for detecting Legionella nucleic acids in a sample |
CA2916352C (en) * | 2013-07-03 | 2020-09-08 | Qvella Corporation | Methods of targeted antibiotic susceptibility testing |
GB201508860D0 (en) | 2015-05-22 | 2015-07-01 | Nat Univ Ireland | Diagnostic method |
CN107012214A (en) * | 2017-03-28 | 2017-08-04 | 西藏自治区农牧科学院农业资源与环境研究所 | A kind of method of mycoplasma in quick detection milk frozen cattle semens |
CN114302968A (en) * | 2019-08-29 | 2022-04-08 | 美国西门子医学诊断股份有限公司 | Reagents and methods for detecting AAV shedding |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4038804A1 (en) * | 1990-10-09 | 1992-04-16 | Boehringer Mannheim Gmbh | METHOD FOR GENUS AND / AND SPECIES-SPECIFIC DETECTION OF BACTERIA IN A SAMPLING LIQUID |
IE81145B1 (en) * | 1989-04-20 | 2000-05-03 | Thomas Gerard Barry | Generation of specific probes for target nucleotide sequences |
GB9020621D0 (en) * | 1990-09-21 | 1990-10-31 | Imp Cancer Res Tech | Identification of organisms |
US5550040A (en) * | 1993-06-23 | 1996-08-27 | Hoffman-La Roche Inc. | Method, reagents and kits for the detection of Neisseria gonorrhoeae |
CA2265895A1 (en) * | 1996-09-05 | 1998-03-12 | The Government Of The United States Of America, Represented By The Secre Tary, Department Of Health And Human Services | Nucleic acid assay for the detection and differentiation of three chlamydia species |
US5849497A (en) * | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
WO1998048008A1 (en) * | 1997-04-23 | 1998-10-29 | Plueckthun Andreas | Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules |
US6096501A (en) * | 1997-11-04 | 2000-08-01 | Becton Dickinson And Company | Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis crytpic plasmid |
US6004754A (en) * | 1998-01-21 | 1999-12-21 | Becton Dickinson And Company | DNA sequence, related probes and primers for the detection of Streptococcus agalactiae |
US7115366B1 (en) * | 1999-04-07 | 2006-10-03 | University Of Utah Research Foundation | Eubacterial tmRNA sequences and uses thereof |
WO2000059918A1 (en) * | 1999-04-07 | 2000-10-12 | University Of Utah Research Foundation | EUBACTERIAL tmRNA SEQUENCES AND USES THEREOF |
JP4527621B2 (en) * | 2005-07-19 | 2010-08-18 | 株式会社小糸製作所 | Vehicle lighting |
US20090246764A1 (en) * | 2008-03-31 | 2009-10-01 | National University Of Ireland Galway | Method for the detection of group B Streptococcus (GBS) (Streptococcus agalactiae) in mammals |
-
2000
- 2000-05-15 ES ES10160953T patent/ES2380577T3/en not_active Expired - Lifetime
- 2000-05-15 WO PCT/IE2000/000066 patent/WO2000070086A1/en active Application Filing
- 2000-05-15 ES ES00925552T patent/ES2345243T3/en not_active Expired - Lifetime
- 2000-05-15 MX MXPA01011635A patent/MXPA01011635A/en active IP Right Grant
- 2000-05-15 CN CN00807503.4A patent/CN1350592B/en not_active Expired - Lifetime
- 2000-05-15 JP JP2000618491A patent/JP2003526334A/en active Pending
- 2000-05-15 CN CN2012105808792A patent/CN103255206A/en active Pending
- 2000-05-15 CA CA2373290A patent/CA2373290C/en not_active Expired - Lifetime
- 2000-05-15 AT AT10160953T patent/ATE541948T1/en active
- 2000-05-15 AT AT00925552T patent/ATE466101T1/en active
- 2000-05-15 EP EP00925552A patent/EP1179090B1/en not_active Expired - Lifetime
- 2000-05-15 US US09/959,964 patent/US7972777B1/en not_active Expired - Fee Related
- 2000-05-15 AT AT10160954T patent/ATE541947T1/en active
- 2000-05-15 DE DE60044294T patent/DE60044294D1/en not_active Expired - Lifetime
- 2000-05-15 EP EP10160954A patent/EP2230317B1/en not_active Expired - Lifetime
- 2000-05-15 ES ES10160954T patent/ES2380567T3/en not_active Expired - Lifetime
- 2000-05-15 EP EP10184687A patent/EP2365097A1/en not_active Withdrawn
- 2000-05-15 EP EP10184640A patent/EP2360276A1/en not_active Withdrawn
- 2000-05-15 AU AU44271/00A patent/AU4427100A/en not_active Abandoned
- 2000-05-15 EP EP10160953A patent/EP2236629B1/en not_active Expired - Lifetime
-
2001
- 2001-11-13 ZA ZA200109333A patent/ZA200109333B/en unknown
-
2010
- 2010-05-03 US US12/772,741 patent/US20120258453A1/en not_active Abandoned
- 2010-05-03 US US12/772,485 patent/US20110111398A1/en not_active Abandoned
- 2010-09-27 JP JP2010216091A patent/JP2011062202A/en not_active Withdrawn
-
2013
- 2013-03-04 US US13/784,121 patent/US8946127B2/en not_active Expired - Fee Related
-
2014
- 2014-05-07 JP JP2014096195A patent/JP5902749B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US7972777B1 (en) | 2011-07-05 |
ES2380567T3 (en) | 2012-05-16 |
MXPA01011635A (en) | 2003-09-10 |
AU4427100A (en) | 2000-12-05 |
CN1350592B (en) | 2014-02-19 |
EP2230317A1 (en) | 2010-09-22 |
JP5902749B2 (en) | 2016-04-13 |
EP2236629A1 (en) | 2010-10-06 |
DE60044294D1 (en) | 2010-06-10 |
ES2380577T3 (en) | 2012-05-16 |
ATE541947T1 (en) | 2012-02-15 |
EP2360276A1 (en) | 2011-08-24 |
US20120258453A1 (en) | 2012-10-11 |
EP2236629B1 (en) | 2012-01-18 |
US20110111398A1 (en) | 2011-05-12 |
ATE541948T1 (en) | 2012-02-15 |
US8946127B2 (en) | 2015-02-03 |
CN1350592A (en) | 2002-05-22 |
CN103255206A (en) | 2013-08-21 |
EP1179090B1 (en) | 2010-04-28 |
ES2345243T3 (en) | 2010-09-20 |
CA2373290C (en) | 2012-04-17 |
ATE466101T1 (en) | 2010-05-15 |
WO2000070086A9 (en) | 2001-04-19 |
EP2365097A1 (en) | 2011-09-14 |
EP2230317B1 (en) | 2012-01-18 |
CA2373290A1 (en) | 2000-11-23 |
EP1179090A1 (en) | 2002-02-13 |
US20130296179A1 (en) | 2013-11-07 |
JP2014195456A (en) | 2014-10-16 |
WO2000070086A1 (en) | 2000-11-23 |
JP2003526334A (en) | 2003-09-09 |
JP2011062202A (en) | 2011-03-31 |
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