DE69533660D1 - Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits - Google Patents

Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits

Info

Publication number
DE69533660D1
DE69533660D1 DE69533660T DE69533660T DE69533660D1 DE 69533660 D1 DE69533660 D1 DE 69533660D1 DE 69533660 T DE69533660 T DE 69533660T DE 69533660 T DE69533660 T DE 69533660T DE 69533660 D1 DE69533660 D1 DE 69533660D1
Authority
DE
Germany
Prior art keywords
detection
nucleic acids
double
stranded nucleic
fluorescent dyes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69533660T
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English (en)
Other versions
DE69533660T2 (de
Inventor
John W Sutherland
David R Patterson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Johnson and Johnson Clinical Diagnostics Inc
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Application filed by Johnson and Johnson Clinical Diagnostics Inc filed Critical Johnson and Johnson Clinical Diagnostics Inc
Publication of DE69533660D1 publication Critical patent/DE69533660D1/de
Application granted granted Critical
Publication of DE69533660T2 publication Critical patent/DE69533660T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Luminescent Compositions (AREA)
DE69533660T 1994-04-29 1995-04-28 Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits Expired - Lifetime DE69533660T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23539694A 1994-04-29 1994-04-29
US235396 1994-04-29

Publications (2)

Publication Number Publication Date
DE69533660D1 true DE69533660D1 (de) 2004-11-25
DE69533660T2 DE69533660T2 (de) 2005-11-10

Family

ID=22885321

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69533660T Expired - Lifetime DE69533660T2 (de) 1994-04-29 1995-04-28 Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits

Country Status (8)

Country Link
US (1) US5563037A (de)
EP (1) EP0684316B1 (de)
JP (1) JP4260903B2 (de)
KR (1) KR100388732B1 (de)
CN (1) CN1100884C (de)
AT (1) ATE280243T1 (de)
CA (1) CA2148140C (de)
DE (1) DE69533660T2 (de)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
US7081226B1 (en) 1996-06-04 2006-07-25 University Of Utah Research Foundation System and method for fluorescence monitoring
US7273749B1 (en) * 1990-06-04 2007-09-25 University Of Utah Research Foundation Container for carrying out and monitoring biological processes
US6180403B1 (en) * 1999-10-28 2001-01-30 Isis Pharmaceuticals Inc. Antisense inhibition of tumor necrosis factor alpha converting enzyme (TACE) expression
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO1997044486A1 (en) * 1996-05-17 1997-11-27 E.I. Du Pont De Nemours And Company Identification of target bacteria by fluorescence detection of primer directed amplification products
SE506700C2 (sv) * 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
CA2257109C (en) * 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US6312930B1 (en) 1996-09-16 2001-11-06 E. I. Du Pont De Nemours And Company Method for detecting bacteria using PCR
AU6534898A (en) * 1997-02-14 1998-09-08 E.I. Du Pont De Nemours And Company Detection of double-stranded dna in a homogeneous solution
ATE426456T1 (de) 1998-05-01 2009-04-15 Gen Probe Inc Automatische diagnostische analysevorrichtung
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
AU2586001A (en) 1999-12-21 2001-07-03 Ortho-Clinical Diagnostics, Inc. Detection of nucleic acids
US7354707B2 (en) 2000-06-27 2008-04-08 National Institute Of Advanced Industrial Science And Technology Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
WO2002014548A1 (en) * 2000-08-10 2002-02-21 Applied Gene Technologies, Inc. Compositions and methods for nucleic acids sample processing and amplification
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
ATE384139T1 (de) * 2002-02-12 2008-02-15 Univ Utah Res Found Multi-test-analyse von echzeit- nukleinsäureamplifikationen
WO2004027035A2 (en) * 2002-09-20 2004-04-01 Yale University Riboswitches, methods for their use, and compositions for use with riboswitches.
US9850381B2 (en) * 2002-10-23 2017-12-26 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
AU2003286573B2 (en) * 2002-10-23 2009-09-10 Idaho Technology, Inc. Amplicon melting analysis with saturation dyes
US20050037369A1 (en) * 2002-10-31 2005-02-17 Neote Kuldeep Singh Panels of molecular targets differentially expressed during CD8+ T cell priming, and methods for therapy and diagnosis utilizing the same
KR20050083971A (ko) * 2002-11-22 2005-08-26 더 존스 홉킨스 유니버시티 인지능 손상의 치료를 위한 표적
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
WO2004058987A2 (en) 2002-12-20 2004-07-15 Qiagen Gmbh Nucleic acid amplification
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
EP1710587B1 (de) * 2003-08-20 2016-08-03 Sysmex Corporation Nukleinsäuredetekionsverfahren
KR100906749B1 (ko) * 2004-03-25 2009-07-09 (주)바이오니아 인터컬레이팅 형광염료가 표지된 프로브를 이용한 핵산 증폭 측정 방법
US9657347B2 (en) 2004-04-20 2017-05-23 University of Utah Research Foundation and BioFire Defense, LLC Nucleic acid melting analysis with saturation dyes
US7387887B2 (en) * 2004-04-20 2008-06-17 University Of Utah Research Foundation Nucleic acid melting analysis with saturation dyes
US7776567B2 (en) 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
US20060275798A1 (en) * 2005-04-04 2006-12-07 Steichen John C Detection of organisms using a media sachet and primer directed nucleic acid amplification
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
US20060246513A1 (en) * 2005-05-02 2006-11-02 Bohannon Robert C Method and device to detect the presence of analytes in a sample
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7781165B2 (en) * 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
WO2007100412A2 (en) * 2005-12-21 2007-09-07 Yale University Methods and compositions related to the modulation of riboswitches
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
US20100137440A1 (en) * 2006-09-11 2010-06-03 Yale University Lysine riboswitches, structure-based compound design with lysine riboswitches, and methods and compositions for use of and with lysine riboswitches
KR20100015757A (ko) 2007-03-22 2010-02-12 예일 유니버시티 택일적 스플라이싱을 조절하는 리보스위치와 관련된 방법 및 조성물
KR20100017905A (ko) 2007-05-29 2010-02-16 예일 유니버시티 리보스위치, 리보스위치의 사용 방법 및 리보스위치를 함유하는 조성물
CA2687684A1 (en) * 2007-05-29 2008-12-11 Yale University Methods and compositions related to riboswitches that control alternative splicing and rna processing
AU2010208386B2 (en) 2009-01-27 2016-08-11 Qiagen Gaithersburg Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection
WO2010132665A1 (en) 2009-05-15 2010-11-18 Yale University Gemm riboswitches, structure-based compound design with gemm riboswitches, and methods and compositions for use of and with gemm riboswitches
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
WO2011088076A2 (en) 2010-01-12 2011-07-21 Yale University Structured rna motifs and compounds and methods for their use
WO2011150227A1 (en) 2010-05-26 2011-12-01 Qiagen Gaithersburg, Inc. Quantitative helicase assay
EP2752668A3 (de) 2010-07-23 2014-10-15 Beckman Coulter, Inc. System oder Verfahren zur Aufnahme analytischer Einheiten
US20130143955A1 (en) 2010-08-09 2013-06-06 Yale University Cyclic di-GMP-II Riboswitches, Motifs, and Compounds, and Methods for Their Use
CN102095705A (zh) * 2010-12-09 2011-06-15 山东理工大学 一种基于激光扫描共聚焦显微镜的头发丝成像方法
US9580713B2 (en) 2011-09-17 2017-02-28 Yale University Fluoride-responsive riboswitches, fluoride transporters, and methods of use
JP6062449B2 (ja) 2011-11-07 2017-01-18 ベックマン コールター， インコーポレイテッド 標本コンテナ検出
KR102040996B1 (ko) 2011-11-07 2019-11-05 베크만 컬터, 인코포레이티드 로봇식 아암
BR112014011046A2 (pt) 2011-11-07 2017-06-13 Beckman Coulter, Inc. fluxo de trabalho e sistema de centrífuga
US9910054B2 (en) 2011-11-07 2018-03-06 Beckman Coulter, Inc. System and method for processing samples
BR112014011044A2 (pt) 2011-11-07 2017-04-25 Beckman Coulter Inc amortecimento magnético para sistema de transporte de espécime
JP6190380B2 (ja) 2011-11-07 2017-08-30 ベックマン コールター， インコーポレイテッド 等分機システムおよびワークフロー
US9428792B2 (en) 2013-03-14 2016-08-30 Certirx Corporation Nucleic acid-based authentication and identification codes
CN106146398B (zh) * 2016-06-13 2019-05-17 苏州宇恒生物科技有限公司 一种化合物及其作为低迁移率核酸染料的应用
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
CA1155041A (en) * 1980-04-21 1983-10-11 Michael E. Jolley Fluorescent nucleic acid stains
US4544546A (en) * 1980-04-21 1985-10-01 Abbott Laboratories Fluorescent nucleic acid stains
US4777129A (en) * 1983-12-12 1988-10-11 Molecular Diagnostics, Inc. Nucleic acid probe detectable by specific nucleic acid binding protein
US5026840A (en) * 1985-01-10 1991-06-25 Molecular Diagnostics, Inc. Photochemical nucleic acid-labeling reagent having a polyalklamine spacer
US4957870A (en) * 1985-11-01 1990-09-18 Becton, Dickinson And Company Detection of Reticulocytes, RNA and DNA
US4883867A (en) * 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US5242797A (en) * 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
US5047321A (en) * 1988-06-15 1991-09-10 Becton Dickinson & Co. Method for analysis of cellular components of a fluid
US4937198A (en) * 1989-07-28 1990-06-26 Becton, Dickinson And Company Novel fluorescent dye
US5049490A (en) * 1990-02-20 1991-09-17 Eastman Kodak Co. Quantitative determination of a DNA polymerase and a test kit useful in same
US5312921A (en) * 1990-03-14 1994-05-17 Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
AU7579891A (en) * 1990-03-14 1991-10-10 Regents Of The University Of California, The Multichromophore fluorescent probes
DE69128520T2 (de) * 1990-10-31 1998-07-09 Tosoh Corp Verfahren zum Nachweis oder Quantifizierung von Zielnukleinsäuren
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
JPH0540097A (ja) * 1991-08-06 1993-02-19 Hitachi Chem Co Ltd 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬
US5316906A (en) * 1991-08-23 1994-05-31 Molecular Probes, Inc. Enzymatic analysis using substrates that yield fluorescent precipitates
CA2119126C (en) * 1991-09-16 1996-09-03 Stephen T. Yue Dimers of unsymmetrical cyanine dyes
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain

Also Published As

Publication number Publication date
EP0684316A1 (de) 1995-11-29
CN1114359A (zh) 1996-01-03
EP0684316B1 (de) 2004-10-20
CA2148140A1 (en) 1995-10-30
ATE280243T1 (de) 2004-11-15
JPH0889299A (ja) 1996-04-09
KR950032641A (ko) 1995-12-22
US5563037A (en) 1996-10-08
DE69533660T2 (de) 2005-11-10
CA2148140C (en) 2010-08-10
CN1100884C (zh) 2003-02-05
JP4260903B2 (ja) 2009-04-30
KR100388732B1 (ko) 2004-09-04

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