ATE151112T1 - Bei hoher temperatur aktive reverse transkriptasen - Google Patents

Bei hoher temperatur aktive reverse transkriptasen

Info

Publication number
ATE151112T1
ATE151112T1 AT91904087T AT91904087T ATE151112T1 AT E151112 T1 ATE151112 T1 AT E151112T1 AT 91904087 T AT91904087 T AT 91904087T AT 91904087 T AT91904087 T AT 91904087T AT E151112 T1 ATE151112 T1 AT E151112T1
Authority
AT
Austria
Prior art keywords
active
high temperature
reverse transcriptases
reverse transcription
rna
Prior art date
Application number
AT91904087T
Other languages
English (en)
Inventor
David H Gelfand
Thomas W Myers
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27412639&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE151112(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/455,611 external-priority patent/US5322770A/en
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE151112T1 publication Critical patent/ATE151112T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
AT91904087T 1989-12-22 1990-12-21 Bei hoher temperatur aktive reverse transkriptasen ATE151112T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US45596789A 1989-12-22 1989-12-22
US07/455,611 US5322770A (en) 1989-12-22 1989-12-22 Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US58547190A 1990-09-20 1990-09-20
PCT/US1990/007641 WO1991009944A2 (en) 1989-12-22 1990-12-21 High temperature reverse transcriptases

Publications (1)

Publication Number Publication Date
ATE151112T1 true ATE151112T1 (de) 1997-04-15

Family

ID=27412639

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91904087T ATE151112T1 (de) 1989-12-22 1990-12-21 Bei hoher temperatur aktive reverse transkriptasen

Country Status (10)

Country Link
EP (1) EP0506889B1 (de)
JP (1) JP2968585B2 (de)
AT (1) ATE151112T1 (de)
AU (1) AU656315B2 (de)
CA (1) CA2071213C (de)
DE (1) DE69030386T2 (de)
DK (1) DK0506889T3 (de)
ES (1) ES2100945T3 (de)
GR (1) GR3023862T3 (de)
WO (1) WO1991009944A2 (de)

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US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
WO1992006200A1 (en) * 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5561058A (en) * 1986-08-22 1996-10-01 Hoffmann-La Roche Inc. Methods for coupled high temperatures reverse transcription and polymerase chain reactions
DE553264T1 (de) * 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
CA2038839A1 (en) * 1991-03-22 1992-09-23 Jiang J. Liu Method for detection of viral rna
AU662906B2 (en) * 1991-06-26 1995-09-21 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
DE69223562T2 (de) * 1991-08-27 1998-06-04 Hoffmann La Roche Verfahren und Reagenzien zum Nachweis von Hepatitis C
FR2690691B1 (fr) * 1992-04-29 1999-02-12 Bio Merieux Procede d'amplification d'arn necessitant une seule etape de manipulation.
USRE39031E1 (en) * 1992-04-29 2006-03-21 Biomerieux RNA amplification method requiring only one manipulation step
ATE205259T1 (de) * 1993-07-01 2001-09-15 Hoffmann La Roche Reagenz und verfahren für reverse transkription bei hoher temperatur, verbunden mit einer polymerase-kettenreaktion
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
WO1995015399A1 (fr) * 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
EP0736609A3 (de) * 1995-04-08 1998-07-29 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
CA2222744C (en) * 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
JP4073038B2 (ja) * 1995-12-15 2008-04-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション テルモアナエロバクター・テルモヒドロスルフリカスからの熱安定性ｄｎａポリメラーゼおよびエキソヌクレアーゼ活性が除去されているその変異体酵素
CA2199213C (en) * 1996-03-11 2007-04-17 John Wesley Backus Amplifying and detecting target nucleic acids using a post amplification incubation step
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
ES2121698B1 (es) * 1996-12-02 1999-07-01 Inia Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
US6030814A (en) * 1997-04-21 2000-02-29 Epicentre Technologies Corporation Reverse transcription method
EP0981609B1 (de) * 1997-05-13 2001-09-19 Azign Bioscience A/S Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)
EP0922765B1 (de) * 1997-12-02 2005-11-23 Roche Diagnostics GmbH Modifizierte DNA Polymerase aus Corboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-Kettenreaktion
EP0921196A1 (de) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modifizierte DNA Polymerase aus Carboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-kettenreaktion
WO1999047539A1 (en) * 1998-03-18 1999-09-23 Amersham Pharmacia Biotech, Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
KR20000072141A (ko) * 2000-08-04 2000-12-05 김유삼 신규한 호열성 단백질 분해효소의 ｄｎａ 서열 및그로부터 유래된 단백질
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
US7504240B2 (en) * 2005-03-10 2009-03-17 Masanori Hirano Methods for synthesizing polynucleotides using a single primer
JP5191041B2 (ja) 2007-04-05 2013-04-24 エフ．ホフマン−ラ ロシュ アーゲー 急速ワンステップｒｔ−ｐｃｒ
KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
BR112012013664B1 (pt) 2009-12-10 2020-11-10 Turnstone Limited Partnership rabdovírus oncolítico
WO2012167382A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
CN104662159B (zh) * 2012-06-08 2021-10-26 爱奥尼安技术公司 核苷酸扩增反应
CN105658790A (zh) 2013-02-21 2016-06-08 东安大略研究所儿童医院有限公司 疫苗组合物
US10633697B2 (en) 2014-01-31 2020-04-28 Qiagen Gmbh Caution chelator hot start
WO2016160965A1 (en) 2015-03-30 2016-10-06 Rubicon Genomics, Inc. Methods and compositions for repair of dna ends by multiple enzymatic activities
US11236384B2 (en) 2017-01-19 2022-02-01 Asuragen, Inc. Methods of RNA amplification
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same
CN110846724B (zh) * 2019-12-03 2023-04-28 江西海普洛斯医学检验实验室有限公司 构建mRNA链特异文库的方法及试剂盒
CN113045410A (zh) * 2021-03-29 2021-06-29 华东理工大学 一种双环降二萜类化合物及其合成基因及制备方法
EP4074839A1 (de) 2021-04-16 2022-10-19 Biotype GmbH Optimierte oligonukleotid-tx-sonde für eine multiplexing-analyse von nukleinsäuren und ein multiplexing-verfahren
EP4350002A1 (de) 2022-10-07 2024-04-10 Biotype GmbH Détection d'analytes moléculaires basée sur la concurrence de sondes sur mesure

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CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme

Also Published As

Publication number Publication date
JPH05505105A (ja) 1993-08-05
GR3023862T3 (en) 1997-09-30
DE69030386D1 (de) 1997-05-07
AU656315B2 (en) 1995-02-02
JP2968585B2 (ja) 1999-10-25
ES2100945T3 (es) 1997-07-01
CA2071213C (en) 2000-05-02
EP0506889B1 (de) 1997-04-02
CA2071213A1 (en) 1991-06-23
WO1991009944A3 (en) 1991-08-22
WO1991009944A2 (en) 1991-07-11
DK0506889T3 (da) 1997-09-22
EP0506889A1 (de) 1992-10-07
AU7244491A (en) 1991-07-24
DE69030386T2 (de) 1997-10-09

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Legal Events

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UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time