ES2169736T3 - Amplificacion de largas secuencias de acidos nucleicos mediante pcr. - Google Patents

Amplificacion de largas secuencias de acidos nucleicos mediante pcr.

Info

Publication number
ES2169736T3
ES2169736T3 ES95102141T ES95102141T ES2169736T3 ES 2169736 T3 ES2169736 T3 ES 2169736T3 ES 95102141 T ES95102141 T ES 95102141T ES 95102141 T ES95102141 T ES 95102141T ES 2169736 T3 ES2169736 T3 ES 2169736T3
Authority
ES
Spain
Prior art keywords
dna polymerase
polymerase
pcr
extension
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95102141T
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English (en)
Inventor
Suzanne Cheng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2169736T3 publication Critical patent/ES2169736T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PROPORCIONAN METODOS Y REACTIVOS PARA LA AMPLIFICACION DE SECUENCIAS ACIDO NUCLEICAS, P. EJ. SECUENCIAS DNA, MAS LARGAS DE 10 KILOBASES POR LA REACCION DE CADENA DE POLIMERASA (RCP). LOS METODOS USAN COMPOSICIONES QUE CONSISTEN DE UNA POLIMERASA PRIMARIA DE DNA TERMOESTABLE DE THERMUS THERMOPHILLUS COMBINADA CON UNA CANTIDAD MENOR DE UNA POLIMERASA SECUNDARIA DE DNA TERMOESTABLE, QUE POSEE UNA ACTIVIDAD DE EXONUCLEASA DE 3'' A 5'', DE THERMOCOCCUS LITORALIS, ESPECIES DE PYROCOCCUS GB-D O THERMOTOGA MARITIMA. LAS COMPOSICIONES DE POLIMERASA DE DNA, CUANDO SE USAN CON EL REGULADOR DE REACCION DESCRITO, PERMITEN AMPLIFICACIONES DE SECUENCIAS DNA HASTA AL MENOS 42,2 KILOBASES EN LONGITUD.
ES95102141T 1994-02-25 1995-02-16 Amplificacion de largas secuencias de acidos nucleicos mediante pcr. Expired - Lifetime ES2169736T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/203,198 US5512462A (en) 1994-02-25 1994-02-25 Methods and reagents for the polymerase chain reaction amplification of long DNA sequences

Publications (1)

Publication Number Publication Date
ES2169736T3 true ES2169736T3 (es) 2002-07-16

Family

ID=22752923

Family Applications (2)

Application Number Title Priority Date Filing Date
ES01113936T Expired - Lifetime ES2256118T3 (es) 1994-02-25 1995-02-16 Amplificacion de secuencias lartgas de acido nucleico con pcr.
ES95102141T Expired - Lifetime ES2169736T3 (es) 1994-02-25 1995-02-16 Amplificacion de largas secuencias de acidos nucleicos mediante pcr.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES01113936T Expired - Lifetime ES2256118T3 (es) 1994-02-25 1995-02-16 Amplificacion de secuencias lartgas de acido nucleico con pcr.

Country Status (9)

Country Link
US (1) US5512462A (es)
EP (2) EP0669401B1 (es)
JP (2) JPH0838198A (es)
AT (2) ATE211179T1 (es)
CA (1) CA2143229A1 (es)
DE (2) DE69534728T2 (es)
DK (1) DK0669401T3 (es)
ES (2) ES2256118T3 (es)
PT (1) PT669401E (es)

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