NO931709L - Nukleinsyreamplifisering ved hjelp av to-enzymers, selvunderholdende sekvensreplikasjon - Google Patents
Nukleinsyreamplifisering ved hjelp av to-enzymers, selvunderholdende sekvensreplikasjonInfo
- Publication number
- NO931709L NO931709L NO93931709A NO931709A NO931709L NO 931709 L NO931709 L NO 931709L NO 93931709 A NO93931709 A NO 93931709A NO 931709 A NO931709 A NO 931709A NO 931709 L NO931709 L NO 931709L
- Authority
- NO
- Norway
- Prior art keywords
- dna
- methods
- activity
- rnase
- dependent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Abstract
Nye metoder for nukleinsyreamplifisering ved hjelp av kontinuerlig, hovedsakelig isotermal selvunderholdende sekvensreplikasjon ("3SR") under anvendelse av RNA-avhengig DNA polymeraseaktivitet, DNA-avhengig DNA polymeraseaktivitet, RNAse H aktivitet og DNA-avhengig RNA polymeraseaktivitet. Ved en av metodene, før enzymatiske aktiviteter kan tilveiebringes ved hjelp av bare to enzymer, anvendes en revers transkriptase og en DNA-avhengig RNA polymerase. Metoden kan anvende to eller tre enzymer til å gi de nødvendige enzyma- tiske aktiviteter. I enkelte av metodene anvendes således en eksogen kilde for RNAse H, som E. coli RNAse H, i kombinasjon med en revers transkriptase og en DNA-avhengig RNA polymerase. I andre metodene i samsvar med den foreliggende oppfinnelse anvendes reaksjonsmedia hvori den inherente RNAse H aktivitet av retrovirale reverse transkriptaser er effektiv til å tilveiebringe høye amplifiseringsnivåer, slik at bare to enzymer, revers transkriptase og DNA-avhengig RNA polymerase er nødvendig. Nye blandinger for gjennomføring av metodene i samsvar med oppfinnelsen er også anført.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61268890A | 1990-11-13 | 1990-11-13 | |
| PCT/US1991/008488 WO1992008800A1 (en) | 1990-11-13 | 1991-11-13 | Nucleic acid amplification by two-enzyme, self-sustained sequence replication |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO931709D0 NO931709D0 (no) | 1993-05-11 |
| NO931709L true NO931709L (no) | 1993-07-12 |
Family
ID=24454235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO93931709A NO931709L (no) | 1990-11-13 | 1993-05-11 | Nukleinsyreamplifisering ved hjelp av to-enzymers, selvunderholdende sekvensreplikasjon |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0572417A4 (no) |
| JP (1) | JPH06502767A (no) |
| AU (1) | AU9131591A (no) |
| CA (1) | CA2096013A1 (no) |
| FI (1) | FI932144A (no) |
| HU (1) | HUT69772A (no) |
| IE (1) | IE913930A1 (no) |
| IL (1) | IL100040A (no) |
| NO (1) | NO931709L (no) |
| NZ (1) | NZ240574A (no) |
| PT (1) | PT99500A (no) |
| WO (1) | WO1992008800A1 (no) |
| ZA (1) | ZA918965B (no) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0628076A1 (en) * | 1992-11-19 | 1994-12-14 | GINGERAS, Thomas R. | PRODUCTION OF MONOCLONAL RECOMBINANT ANTIBODIES WITHOUT THE USE OF HYBRIDOMAS BY $i(IN VITRO) SPLEEN FRAGMENT CULTURE COMBINED WITH ISOTHERMAL SELF-SUSTAINED SEQUENCE REPLICATION OF RNA |
| EP0682121B1 (en) | 1993-12-01 | 2002-08-28 | Toyo Boseki Kabushiki Kaisha | Method of amplifying and detecting target nucleic acid sequence by using thermostable enzymes |
| US5705366A (en) * | 1994-09-15 | 1998-01-06 | Johnson & Johnson Clinical Diagnostics, Inc. | Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor |
| JPH08131171A (ja) * | 1994-11-11 | 1996-05-28 | Asahi Chem Ind Co Ltd | 逆転写酵素の安定化組成物 |
| US5763184A (en) | 1996-01-30 | 1998-06-09 | Roche Molecular Systems, Inc. | Nucleotide sequence variation in the ABO glycosyltransferase gene |
| WO2000000638A2 (en) * | 1998-06-26 | 2000-01-06 | Akzo Nobel N.V. | Tagging of rna amplicons generated by transcription-based amplification |
| JP2001029078A (ja) * | 1999-07-16 | 2001-02-06 | Shimadzu Corp | Ｒｎａ増幅法 |
| EP1307469B1 (en) | 2000-08-03 | 2008-01-23 | Boehringer Mannheim Gmbh | Nucleic acid binding compounds containing pyrazolo¬3,4-d pyrimidine analogues of purin-2,6-diamine and their uses |
| US6780588B2 (en) | 2001-05-07 | 2004-08-24 | Applera Corporation | Methods for the reduction of stutter in microsatellite amplification |
| US6841349B2 (en) | 2001-05-07 | 2005-01-11 | Applera Corporation Applied Biosystems Group | Methods for the reduction of stutter in microsatellite amplification |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US9777312B2 (en) | 2001-06-30 | 2017-10-03 | Enzo Life Sciences, Inc. | Dual polarity analysis of nucleic acids |
| US20040161741A1 (en) | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
| EP1466018B2 (en) | 2002-01-08 | 2015-08-12 | Roche Diagnostics GmbH | Use of silica material in an amplification reaction |
| AU2003208959A1 (en) | 2002-01-30 | 2003-09-02 | Id Biomedical Corporation | Methods for detecting vancomycin-resistant microorganisms and compositions therefor |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| DE10240868A1 (de) * | 2002-09-04 | 2004-03-18 | Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH | Verbesserte Verfahren zur Synthese von Nukleinsäuren |
| JP2006516391A (ja) | 2003-01-29 | 2006-07-06 | エフ．ホフマン−ラ ロシュ アーゲー | 重亜硫酸塩処理の改良された方法 |
| JP2004357502A (ja) * | 2003-05-30 | 2004-12-24 | Olympus Corp | 核酸分子を使用した情報処理方法 |
| US20090232771A1 (en) | 2004-07-13 | 2009-09-17 | Takeda Pharmaceutical Company Limited | Method of controlling cell functions |
| EP1632578A1 (en) | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | DNA decontamination method |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| EP2700723B1 (en) | 2007-08-06 | 2015-07-15 | Orion Genomics, LLC | Novel single nucleotide polymorphisms and combinations of novel and known polymorphisms for determining the allele-specific expression of the IGF2 gene |
| CA2639416C (en) | 2007-09-11 | 2019-12-31 | F. Hoffmann-La Roche Ag | Diagnostic test for susceptibility to b-raf kinase inhibitors |
| US20100324154A1 (en) | 2007-11-01 | 2010-12-23 | Hageman Gregory S | Assessing susceptibility to vascular disorders |
| CA2751758A1 (en) | 2009-02-11 | 2010-08-19 | Orion Genomics Llc | Combinations of polymorphisms for determining allele-specific expression of igf2 |
| WO2011128096A1 (en) | 2010-04-16 | 2011-10-20 | Roche Diagnostics Gmbh | Polymorphism markers for predicting response to interleukin-6 receptor-inhibiting monoclonal antibody drug treatment |
| PL2576837T3 (pl) | 2010-06-04 | 2018-04-30 | Chronix Biomedical | Krążeniowe biomarkery typu kwasu nukleinowego związane z rakiem prostaty |
| WO2012040619A2 (en) | 2010-09-24 | 2012-03-29 | Massachusetts Eye And Ear Infirmary | Methods and compositions for prognosing and/or detecting age-related macular degeneration |
| US9458484B2 (en) | 2010-10-22 | 2016-10-04 | Bio-Rad Laboratories, Inc. | Reverse transcriptase mixtures with improved storage stability |
| WO2013030168A1 (en) | 2011-08-31 | 2013-03-07 | F. Hoffmann-La Roche Ag | Method for predicting risk of hypertension associated with anti-angiogenesis therapy |
| MX2014002311A (es) | 2011-08-31 | 2014-08-26 | Hoffmann La Roche | Capacidad de respuesta a inhibidores de angiogenesis. |
| AU2012312515A1 (en) | 2011-09-19 | 2014-03-13 | Genentech, Inc. | Combination treatments comprising c-met antagonists and B-raf antagonists |
| US20140303008A1 (en) | 2011-10-21 | 2014-10-09 | Chronix Biomedical | Colorectal cancer associated circulating nucleic acid biomarkers |
| EP2783015A1 (en) | 2011-11-23 | 2014-10-01 | F.Hoffmann-La Roche Ag | Responsiveness to angiogenesis inhibitors |
| US9115394B2 (en) | 2011-12-22 | 2015-08-25 | Roche Molecular Systems, Inc. | Methods and reagents for reducing non-specific amplification |
| ES2704682T5 (es) | 2013-05-29 | 2023-11-10 | Chronix Biomedical | Detección y cuantificación de ADN extracelular del donante en la circulación de receptores de trasplantes de órganos |
| SG11201607772WA (en) | 2014-03-31 | 2016-10-28 | Debiopharm Int Sa | Fgfr fusions |
| JP7231326B2 (ja) | 2014-11-10 | 2023-03-01 | ジェネンテック， インコーポレイテッド | Ｉｌ－３３媒介性障害のための治療及び診断方法 |
| CA2983819A1 (en) | 2015-04-24 | 2016-10-27 | Atila Biosystems Incorporated | Amplification with primers of limited nucleotide composition |
| CA2985123C (en) | 2015-08-17 | 2021-04-13 | Antonio Gualberto | Methods of treating cancer patients with farnesyltransferase inhibitors |
| WO2017184968A1 (en) | 2016-04-22 | 2017-10-26 | Kura Oncology, Inc. | Methods of selecting cancer patients for treatment with farnesyltransferase inhibitors |
| MY190861A (en) | 2016-11-03 | 2022-05-12 | Kura Oncology Inc | Farnesyltransferase inhibitors for use in methods of treating cancer |
| MX2019009821A (es) | 2017-02-21 | 2019-12-02 | Kura Oncology Inc | Metodos para tratar cancer con inhibidores de farnesiltransferasa. |
| US9956215B1 (en) | 2017-02-21 | 2018-05-01 | Kura Oncology, Inc. | Methods of treating cancer with farnesyltransferase inhibitors |
| BR112020002591A2 (pt) | 2017-08-07 | 2020-07-28 | Kura Oncology, Inc. | método para o tratamento de câncer |
| US10806730B2 (en) | 2017-08-07 | 2020-10-20 | Kura Oncology, Inc. | Methods of treating cancer with farnesyltransferase inhibitors |
| JP7418337B2 (ja) | 2018-02-09 | 2024-01-19 | ジェネンテック， インコーポレイテッド | マスト細胞媒介性炎症性疾患の治療法及び診断法 |
| CN113286591A (zh) | 2018-11-01 | 2021-08-20 | 库拉肿瘤学公司 | 用法尼基转移酶抑制剂治疗癌症的方法 |
| US20220142983A1 (en) | 2019-03-01 | 2022-05-12 | Kura Oncology, Inc. | Methods of treating cancer with farnesyltransferase inhibitors |
| TW202043487A (zh) | 2019-03-29 | 2020-12-01 | 美商庫拉腫瘤技術股份有限公司 | 以法尼基轉移酶(farnesyltransferase)抑制劑治療鱗狀細胞癌之方法 |
| US20220168296A1 (en) | 2019-04-01 | 2022-06-02 | Kura Oncology, Inc. | Methods of treating cancer with farnesyltransferase inhibitors |
| WO2020223583A1 (en) | 2019-05-02 | 2020-11-05 | Kura Oncology, Inc. | Methods of treating acute myeloid leukemia with farnesyltransferase inhibitors |
| US20230110203A1 (en) | 2020-03-13 | 2023-04-13 | Medimmune Limited | Therapeutic methods for the treatment of subjects with risk alelles in il33 |
| WO2024015331A1 (en) | 2022-07-12 | 2024-01-18 | Genentech, Inc. | Therapeutic and diagnostic methods for multiple sclerosis |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| HU216105B (hu) * | 1988-12-16 | 1999-04-28 | Akzo Nobel N.V. | Amplifikációs eljárás önfenntartó szekvenciareplikációs rendszerrel |
| ATE282716T1 (de) * | 1989-07-11 | 2004-12-15 | Gen Probe Inc | Verfahren zur amplifikation von nukleinsäuresequenzen |
-
1991
- 1991-11-12 IE IE393091A patent/IE913930A1/en not_active Application Discontinuation
- 1991-11-12 IL IL10004091A patent/IL100040A/en not_active IP Right Cessation
- 1991-11-12 ZA ZA918965A patent/ZA918965B/xx unknown
- 1991-11-13 NZ NZ240574A patent/NZ240574A/en unknown
- 1991-11-13 EP EP19920901557 patent/EP0572417A4/en not_active Ceased
- 1991-11-13 HU HU9301369A patent/HUT69772A/hu unknown
- 1991-11-13 CA CA002096013A patent/CA2096013A1/en not_active Abandoned
- 1991-11-13 WO PCT/US1991/008488 patent/WO1992008800A1/en not_active Application Discontinuation
- 1991-11-13 JP JP4502286A patent/JPH06502767A/ja active Pending
- 1991-11-13 PT PT99500A patent/PT99500A/pt not_active Application Discontinuation
- 1991-11-13 AU AU91315/91A patent/AU9131591A/en not_active Abandoned
-
1993
- 1993-05-11 NO NO93931709A patent/NO931709L/no unknown
- 1993-05-12 FI FI932144A patent/FI932144A/fi not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| FI932144A0 (fi) | 1993-05-12 |
| JPH06502767A (ja) | 1994-03-31 |
| WO1992008800A1 (en) | 1992-05-29 |
| ZA918965B (en) | 1992-08-26 |
| IL100040A (en) | 1995-12-31 |
| FI932144A (fi) | 1993-05-12 |
| IE913930A1 (en) | 1992-06-17 |
| HUT69772A (en) | 1995-09-28 |
| PT99500A (pt) | 1992-10-30 |
| EP0572417A1 (en) | 1993-12-08 |
| IL100040A0 (en) | 1992-08-18 |
| NO931709D0 (no) | 1993-05-11 |
| HU9301369D0 (en) | 1993-10-28 |
| EP0572417A4 (en) | 1994-11-23 |
| NZ240574A (en) | 1994-10-26 |
| AU9131591A (en) | 1992-06-11 |
| CA2096013A1 (en) | 1992-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO931709L (no) | Nukleinsyreamplifisering ved hjelp av to-enzymers, selvunderholdende sekvensreplikasjon | |
| ATE151112T1 (de) | Bei hoher temperatur aktive reverse transkriptasen | |
| Moser et al. | Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme | |
| US5498523A (en) | DNA sequencing with pyrophosphatase | |
| Towle et al. | Purification and characterization of bacteriophage gh-I-induced deoxyribonucleic acid-dependent ribonucleic acid polymerase from Pseudomonas putida. | |
| EP2272952B8 (en) | Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity | |
| US20100015683A1 (en) | Thermostable reverse transcriptases and uses thereof | |
| DE69128077D1 (de) | Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen | |
| DE69628959D1 (de) | Stabilisierte Zusammensetzungen von Reverser Transkriptase und RNA Polymerase für Nukleinsäureamplifikation | |
| EP2295551A1 (en) | Thermostable reverse transcriptases and uses thereof | |
| ATE360093T1 (de) | Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure | |
| NO960408L (no) | DNA sekvenseringsenzymer | |
| JP6029636B2 (ja) | Ｒｎａの検出方法 | |
| DK0632134T3 (da) | Reagenser og fremgangsmåder til koblet revers transkription ved høj temperatur og polymerase-kædereaktioner | |
| Baalsrud | Some aspects of the physiology of thiobacilli | |
| DK731988D0 (da) | Amperometrisk fremgangsmaade til kvantitativ bestemmelse af 1,4-dihydronikotinamid-adenin-dinukleotid (nadh) i oploesning | |
| EP0467953A1 (en) | Improved primer extension reactions | |
| DE69737024D1 (en) | Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius | |
| Kaboev et al. | Purification and properties of deoxyribonucleic acid polymerase from Bacillus stearothermophilus | |
| Tuominen et al. | Inhibition of RNA-directed DNA polymerase from Rauscher leukemia virus by the 5′-triphosphate of cytosine arabinoside | |
| Hatahet et al. | In vitro selection of sequence contexts which enhance bypass of abasic sites and tetrahydrofuran by T4 DNA polymerase holoenzyme | |
| WO2022115822A3 (en) | Methods for full-length reverse transcription pcr of long rnas containing modified ribonucleosides using a thermostable reverse transcriptase | |
| ATE311477T1 (de) | Hohe dichte markierung von dns mit modifizierte oder chromophore tragende nukleotide und benutzte dns polymerasen | |
| Vardanis et al. | On the nucleotide specificity of the polynucleotide phosphorylase of the crown-gall tumor-inducing organism Agrobacterium tumefaciens | |
| Grossmann et al. | Cooperative effects of RNA polymerase from higher plant cells and Escherichia coli: a comparison |