NO960408L - DNA sekvenseringsenzymer - Google Patents

DNA sekvenseringsenzymer

Info

Publication number
NO960408L
NO960408L NO960408A NO960408A NO960408L NO 960408 L NO960408 L NO 960408L NO 960408 A NO960408 A NO 960408A NO 960408 A NO960408 A NO 960408A NO 960408 L NO960408 L NO 960408L
Authority
NO
Norway
Prior art keywords
dna
polymerases
dna sequencing
sequencing
family
Prior art date
Application number
NO960408A
Other languages
English (en)
Other versions
NO960408D0 (no
Inventor
Myron F Goodman
Linda J Reha-Krantz
Original Assignee
Univ Southern California
Univ Alberta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Southern California, Univ Alberta filed Critical Univ Southern California
Publication of NO960408D0 publication Critical patent/NO960408D0/no
Publication of NO960408L publication Critical patent/NO960408L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Det er tilveiebragt avvikende familie B DNA polymeraser uten 3'-> 5' eksonukleaseaktivitet. Disse avvikende polymeraser har anvendbarhet som DNA sekvenseringspolymeraser. Fremgangsmåter for DNA sekvensering med familie B DNA polymeraser og kjedetermineringsnukleotider som ikke tidligere er anvendt for sekvensering er blitt utviklet. Disse fremgangsmåter omfatter bruk av familie B DNA polymeraser som inntil nå ikke har vært kjent å kunne anvendes i DNA sekvensering, slik som avvikende eller villtype former av fag T4 DNA polymerase eller Escherichia coli DNA polymerase II, med nye kombinasjoner av deoksynukleotider og kjedetermineringsnukleotider.
NO960408A 1993-08-02 1996-01-31 DNA sekvenseringsenzymer NO960408L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/101,593 US5547859A (en) 1993-08-02 1993-08-02 Chain-terminating nucleotides for DNA sequencing methods
PCT/US1994/008610 WO1995004162A2 (en) 1993-08-02 1994-08-01 Dna sequencing enzymes

Publications (2)

Publication Number Publication Date
NO960408D0 NO960408D0 (no) 1996-01-31
NO960408L true NO960408L (no) 1996-03-27

Family

ID=22285458

Family Applications (1)

Application Number Title Priority Date Filing Date
NO960408A NO960408L (no) 1993-08-02 1996-01-31 DNA sekvenseringsenzymer

Country Status (18)

Country Link
US (3) US5547859A (no)
EP (1) EP0713535A1 (no)
JP (1) JPH09509301A (no)
KR (1) KR960704067A (no)
CN (1) CN1132529A (no)
AU (1) AU699498B2 (no)
BG (1) BG100188A (no)
BR (1) BR9407403A (no)
CA (1) CA2168471A1 (no)
CZ (1) CZ30696A3 (no)
FI (1) FI960469A (no)
HU (1) HUT75841A (no)
LV (1) LV11486B (no)
NO (1) NO960408L (no)
NZ (1) NZ269727A (no)
PL (1) PL312836A1 (no)
SK (1) SK14196A3 (no)
WO (1) WO1995004162A2 (no)

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US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
WO1997039150A1 (en) * 1996-04-15 1997-10-23 University Of Southern California Synthesis of fluorophore-labeled dna
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
WO1999057321A1 (en) * 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
WO2001032887A1 (en) * 1999-10-29 2001-05-10 Stratagene Compositions and methods utilizing dna polymerases
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) * 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB2395954A (en) 2002-08-23 2004-06-09 Solexa Ltd Modified nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
CA2566806A1 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
EP2940029B1 (en) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP2725107B1 (en) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified ddNTPs and nucleic acid comprising inosine with reversible terminators
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7767441B2 (en) * 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
EP2245199B1 (en) 2008-02-01 2013-11-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
EP3865590B1 (en) 2009-09-09 2024-04-10 The General Hospital Corporation Use of microvesicles in analyzing kras mutations
EP2475988B1 (en) 2009-09-09 2018-11-14 The General Hospital Corporation Use of microvesicles in analyzing nucleic acid profiles
WO2012031008A2 (en) 2010-08-31 2012-03-08 The General Hospital Corporation Cancer-related biological materials in microvesicles
AU2011326366B2 (en) 2010-11-10 2017-02-23 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
US20150252428A1 (en) 2012-10-03 2015-09-10 Exosome Diagnostics, Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
ES2685549T3 (es) 2013-03-15 2018-10-09 Illumina Cambridge Limited Nucleósidos o nucleótidos modificados
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US10451544B2 (en) 2016-10-11 2019-10-22 Genotox Laboratories Methods of characterizing a urine sample

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US4666892A (en) * 1984-03-06 1987-05-19 Sloan-Kettering Memorial Cancer Center Method and composition for hepatitis treatment with pyrimidine nucleoside compounds
DE3546374A1 (de) * 1985-12-31 1987-07-02 Max Planck Gesellschaft Verfahren zur sequenzanalyse von dna
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US4935361A (en) * 1986-09-18 1990-06-19 Yale University Cloning and expression of T4 DNA polymerase
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
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DE4214112A1 (de) * 1991-08-02 1993-02-04 Europ Lab Molekularbiolog Neues verfahren zur sequenzierung von nukleinsaeuren
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Also Published As

Publication number Publication date
BR9407403A (pt) 1996-11-05
US5660980A (en) 1997-08-26
SK14196A3 (en) 1996-06-05
LV11486A (lv) 1996-08-20
WO1995004162A3 (en) 1995-06-01
WO1995004162A2 (en) 1995-02-09
US5928919A (en) 1999-07-27
JPH09509301A (ja) 1997-09-22
US5547859A (en) 1996-08-20
EP0713535A1 (en) 1996-05-29
AU699498B2 (en) 1998-12-03
LV11486B (en) 1997-02-20
HUT75841A (en) 1997-05-28
HU9600235D0 (en) 1996-03-28
BG100188A (en) 1996-12-31
CZ30696A3 (en) 1996-06-12
CN1132529A (zh) 1996-10-02
NZ269727A (en) 1997-11-24
CA2168471A1 (en) 1995-02-09
AU7408894A (en) 1995-02-28
FI960469A0 (fi) 1996-02-01
NO960408D0 (no) 1996-01-31
KR960704067A (ko) 1996-08-31
FI960469A (fi) 1996-03-27
PL312836A1 (en) 1996-05-13

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