ATE205259T1 - Reagenz und verfahren für reverse transkription bei hoher temperatur, verbunden mit einer polymerase-kettenreaktion - Google Patents
Reagenz und verfahren für reverse transkription bei hoher temperatur, verbunden mit einer polymerase-kettenreaktionInfo
- Publication number
- ATE205259T1 ATE205259T1 AT94109468T AT94109468T ATE205259T1 AT E205259 T1 ATE205259 T1 AT E205259T1 AT 94109468 T AT94109468 T AT 94109468T AT 94109468 T AT94109468 T AT 94109468T AT E205259 T1 ATE205259 T1 AT E205259T1
- Authority
- AT
- Austria
- Prior art keywords
- reverse transcription
- high temperature
- methods
- divalent cation
- reagent
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 238000010839 reverse transcription Methods 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 title 1
- 239000000872 buffer Substances 0.000 abstract 5
- 230000003321 amplification Effects 0.000 abstract 3
- 150000001768 cations Chemical class 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 239000002184 metal Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8648393A | 1993-07-01 | 1993-07-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE205259T1 true ATE205259T1 (de) | 2001-09-15 |
Family
ID=22198856
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT94109468T ATE205259T1 (de) | 1993-07-01 | 1994-06-20 | Reagenz und verfahren für reverse transkription bei hoher temperatur, verbunden mit einer polymerase-kettenreaktion |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0632134B1 (de) |
| JP (1) | JP3097893B2 (de) |
| AT (1) | ATE205259T1 (de) |
| AU (1) | AU675318B2 (de) |
| CA (1) | CA2127188C (de) |
| DE (1) | DE69428167T2 (de) |
| DK (1) | DK0632134T3 (de) |
| ES (1) | ES2161731T3 (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1251182A3 (de) * | 1993-12-01 | 2002-11-27 | Toyo Boseki Kabushiki Kaisha | Verfahren zur Amplifikation und Erkennung von Nukleinsäure-Zielsequenzen unter Verwendung von thermostabilen Enzymen |
| EP0736609A3 (de) * | 1995-04-08 | 1998-07-29 | Roche Diagnostics GmbH | Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA |
| DE19513728A1 (de) * | 1995-04-11 | 1996-10-31 | Boehringer Mannheim Gmbh | Verfahren zur Reduzierung der Bildung von Artefakten |
| US6077664A (en) * | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
| US6001645A (en) * | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
| US5916779A (en) * | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US5837442A (en) * | 1995-11-29 | 1998-11-17 | Roche Molecular Systems, Inc. | Oligonucleotide primers for amplifying HCV nucleic acid |
| DE19611759A1 (de) * | 1996-03-25 | 1997-10-02 | Boehringer Mannheim Gmbh | Thermostabile DNA-Polymerase aus Thermococcus spec. |
| DE19647055A1 (de) | 1996-11-14 | 1998-05-20 | Boehringer Mannheim Gmbh | Stabilisierte wässrige Nukleosidtriphosphat-Lösungen |
| ES2121698B1 (es) * | 1996-12-02 | 1999-07-01 | Inia | Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna. |
| WO2003048392A1 (en) * | 2001-12-04 | 2003-06-12 | Bio3 Research S.R.L. | Salts mixture for the preparation of pcr buffer solutions |
| GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
| DE10240868A1 (de) * | 2002-09-04 | 2004-03-18 | Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH | Verbesserte Verfahren zur Synthese von Nukleinsäuren |
| EP1594982A4 (de) * | 2003-01-29 | 2006-04-12 | Quanta Biosciences Inc | Verfahren und zusammensetzungen zum nachweis und zur quantifizierung von rna |
| JP2010161935A (ja) * | 2009-01-13 | 2010-07-29 | Fujifilm Corp | 核酸増幅反応におけるばらつきを低減する方法 |
| WO2012170907A2 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| FI3461807T3 (fi) | 2011-06-08 | 2023-09-07 | Life Technologies Corp | Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten |
| EP3539944A1 (de) | 2013-10-25 | 2019-09-18 | Life Technologies Corporation | Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon |
| US11085042B2 (en) * | 2018-04-19 | 2021-08-10 | City University Of Hong Kong | Method of producing DNA and DNA library |
| EP4074839A1 (de) | 2021-04-16 | 2022-10-19 | Biotype GmbH | Optimierte oligonukleotid-tx-sonde für eine multiplexing-analyse von nukleinsäuren und ein multiplexing-verfahren |
| EP4350002A1 (de) | 2022-10-07 | 2024-04-10 | Biotype GmbH | Détection d'analytes moléculaires basée sur la concurrence de sondes sur mesure |
| DE102023124346A1 (de) | 2023-09-09 | 2025-03-13 | Biotype Gmbh | Verfahren zur Detektion des Endometriumkarzinoms Subtyp CN hoch |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5310652A (en) * | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| JP2968585B2 (ja) * | 1989-12-22 | 1999-10-25 | エフ．ホフマン ― ラ ロシュ アーゲー | 高温度逆転写酵素 |
| WO1992001814A2 (en) * | 1990-07-24 | 1992-02-06 | F. Hoffmann-La-Roche Ag | The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases |
| ATE161291T1 (de) * | 1991-08-27 | 1998-01-15 | Hoffmann La Roche | Verfahren und reagenzien zum nachweis von hepatitis c |
-
1994
- 1994-06-20 AT AT94109468T patent/ATE205259T1/de active
- 1994-06-20 DK DK94109468T patent/DK0632134T3/da active
- 1994-06-20 DE DE69428167T patent/DE69428167T2/de not_active Expired - Lifetime
- 1994-06-20 ES ES94109468T patent/ES2161731T3/es not_active Expired - Lifetime
- 1994-06-20 EP EP94109468A patent/EP0632134B1/de not_active Expired - Lifetime
- 1994-06-24 AU AU65946/94A patent/AU675318B2/en not_active Expired
- 1994-06-30 CA CA002127188A patent/CA2127188C/en not_active Expired - Lifetime
- 1994-07-01 JP JP06184220A patent/JP3097893B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0632134B1 (de) | 2001-09-05 |
| JP3097893B2 (ja) | 2000-10-10 |
| DE69428167D1 (de) | 2001-10-11 |
| CA2127188C (en) | 1999-12-07 |
| EP0632134A3 (de) | 1995-12-13 |
| EP0632134A2 (de) | 1995-01-04 |
| JPH0759599A (ja) | 1995-03-07 |
| DE69428167T2 (de) | 2002-05-29 |
| AU675318B2 (en) | 1997-01-30 |
| CA2127188A1 (en) | 1995-01-02 |
| DK0632134T3 (da) | 2001-12-03 |
| AU6594694A (en) | 1995-01-12 |
| ES2161731T3 (es) | 2001-12-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |