DE69428167T2 - Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion - Google Patents

Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion

Info

Publication number
DE69428167T2
DE69428167T2 DE69428167T DE69428167T DE69428167T2 DE 69428167 T2 DE69428167 T2 DE 69428167T2 DE 69428167 T DE69428167 T DE 69428167T DE 69428167 T DE69428167 T DE 69428167T DE 69428167 T2 DE69428167 T2 DE 69428167T2
Authority
DE
Germany
Prior art keywords
reverse transcription
high temperature
methods
divalent cation
chain reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69428167T
Other languages
English (en)
Other versions
DE69428167D1 (de
Inventor
David H Gelfand
Thomas W Myers
Christopher L Sigua
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of DE69428167D1 publication Critical patent/DE69428167D1/de
Publication of DE69428167T2 publication Critical patent/DE69428167T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE69428167T 1993-07-01 1994-06-20 Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion Expired - Lifetime DE69428167T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8648393A 1993-07-01 1993-07-01

Publications (2)

Publication Number Publication Date
DE69428167D1 DE69428167D1 (de) 2001-10-11
DE69428167T2 true DE69428167T2 (de) 2002-05-29

Family

ID=22198856

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69428167T Expired - Lifetime DE69428167T2 (de) 1993-07-01 1994-06-20 Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion

Country Status (8)

Country Link
EP (1) EP0632134B1 (de)
JP (1) JP3097893B2 (de)
AT (1) ATE205259T1 (de)
AU (1) AU675318B2 (de)
CA (1) CA2127188C (de)
DE (1) DE69428167T2 (de)
DK (1) DK0632134T3 (de)
ES (1) ES2161731T3 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10240868A1 (de) * 2002-09-04 2004-03-18 Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH Verbesserte Verfahren zur Synthese von Nukleinsäuren

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015399A1 (fr) * 1993-12-01 1995-06-08 Toyo Boseki Kabushiki Kaisha Procede d'amplification et de detection d'une sequence nucleotidique au moyen d'enzymes thermostables
EP0736609A3 (de) * 1995-04-08 1998-07-29 Roche Diagnostics GmbH Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA
DE19513728A1 (de) * 1995-04-11 1996-10-31 Boehringer Mannheim Gmbh Verfahren zur Reduzierung der Bildung von Artefakten
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5837442A (en) * 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
DE19611759A1 (de) * 1996-03-25 1997-10-02 Boehringer Mannheim Gmbh Thermostabile DNA-Polymerase aus Thermococcus spec.
DE19647055A1 (de) * 1996-11-14 1998-05-20 Boehringer Mannheim Gmbh Stabilisierte wässrige Nukleosidtriphosphat-Lösungen
ES2121698B1 (es) * 1996-12-02 1999-07-01 Inia Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna.
AU2002217427A1 (en) * 2001-12-04 2003-06-17 Bio3 Research S.R.L. Salts mixture for the preparation of pcr buffer solutions
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20040259115A1 (en) * 2003-01-29 2004-12-23 Schuster David M. Methods and compositions for RNA detection and quantitation
JP2010161935A (ja) * 2009-01-13 2010-07-29 Fujifilm Corp 核酸増幅反応におけるばらつきを低減する方法
EP4249603A3 (de) 2011-06-08 2024-01-03 Life Technologies Corporation Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106458885B (zh) 2013-10-25 2019-12-10 生命技术公司 用于聚合酶链式反应系统的新颖化合物及其应用
US11085042B2 (en) * 2018-04-19 2021-08-10 City University Of Hong Kong Method of producing DNA and DNA library
EP4074839A1 (de) 2021-04-16 2022-10-19 Biotype GmbH Optimierte oligonukleotid-tx-sonde für eine multiplexing-analyse von nukleinsäuren und ein multiplexing-verfahren
EP4350002A1 (de) 2022-10-07 2024-04-10 Biotype GmbH Détection d'analytes moléculaires basée sur la concurrence de sondes sur mesure

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) * 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
AU656315B2 (en) * 1989-12-22 1995-02-02 F. Hoffmann-La Roche Ag pigh temperature reverse transcriptases
ATE176002T1 (de) * 1990-07-24 1999-02-15 Hoffmann La Roche Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen
ATE241704T1 (de) * 1991-08-27 2003-06-15 Hoffmann La Roche Primers und sondern zum nachweis von hepatitis c

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10240868A1 (de) * 2002-09-04 2004-03-18 Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH Verbesserte Verfahren zur Synthese von Nukleinsäuren

Also Published As

Publication number Publication date
EP0632134A2 (de) 1995-01-04
CA2127188C (en) 1999-12-07
EP0632134B1 (de) 2001-09-05
JPH0759599A (ja) 1995-03-07
CA2127188A1 (en) 1995-01-02
DE69428167D1 (de) 2001-10-11
AU675318B2 (en) 1997-01-30
ATE205259T1 (de) 2001-09-15
AU6594694A (en) 1995-01-12
ES2161731T3 (es) 2001-12-16
JP3097893B2 (ja) 2000-10-10
DK0632134T3 (da) 2001-12-03
EP0632134A3 (de) 1995-12-13

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