ATE176002T1 - Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen - Google Patents

Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen

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Publication number
ATE176002T1
ATE176002T1 AT91916353T AT91916353T ATE176002T1 AT E176002 T1 ATE176002 T1 AT E176002T1 AT 91916353 T AT91916353 T AT 91916353T AT 91916353 T AT91916353 T AT 91916353T AT E176002 T1 ATE176002 T1 AT E176002T1
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AT
Austria
Prior art keywords
nucleic acid
pct
amplification
vitro
reducing non
Prior art date
Application number
AT91916353T
Other languages
English (en)
Inventor
John J Sninsky
David H Gelfand
Shirley Y Kwok
Original Assignee
Hoffmann La Roche
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Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
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Publication of ATE176002T1 publication Critical patent/ATE176002T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
AT91916353T 1990-07-24 1991-07-23 Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen ATE176002T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55751790A 1990-07-24 1990-07-24
US60915790A 1990-11-02 1990-11-02

Publications (1)

Publication Number Publication Date
ATE176002T1 true ATE176002T1 (de) 1999-02-15

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Family Applications (1)

Application Number Title Priority Date Filing Date
AT91916353T ATE176002T1 (de) 1990-07-24 1991-07-23 Verringerung von nicht spezifischer amplifikation während einer (in vitro) nukleinsäure amplifikation unter verwendung von modifizierten nukleinsäure basen

Country Status (11)

Country Link
US (1) US5418149A (de)
EP (1) EP0540693B1 (de)
JP (1) JP3392863B2 (de)
AT (1) ATE176002T1 (de)
AU (1) AU665338B2 (de)
CA (1) CA2087724C (de)
DE (1) DE69130800T2 (de)
DK (1) DK0540693T3 (de)
ES (1) ES2128323T3 (de)
GR (1) GR3029987T3 (de)
WO (1) WO1992001814A2 (de)

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DE69130800T2 (de) 1999-09-16
AU665338B2 (en) 1996-01-04
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EP0540693B1 (de) 1999-01-20
JPH06501612A (ja) 1994-02-24
AU8532791A (en) 1992-02-18
DE69130800D1 (de) 1999-03-04
CA2087724C (en) 2003-09-16
WO1992001814A3 (en) 1992-05-14
US5418149A (en) 1995-05-23
CA2087724A1 (en) 1992-01-25
ES2128323T3 (es) 1999-05-16

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