DE69307317D1 - Verwendung von Nuklein-säure Analoge in der Inhibition von Nuklein-säure Amplifikation - Google Patents

Verwendung von Nuklein-säure Analoge in der Inhibition von Nuklein-säure Amplifikation

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Publication number
DE69307317D1
DE69307317D1 DE69307317T DE69307317T DE69307317D1 DE 69307317 D1 DE69307317 D1 DE 69307317D1 DE 69307317 T DE69307317 T DE 69307317T DE 69307317 T DE69307317 T DE 69307317T DE 69307317 D1 DE69307317 D1 DE 69307317D1
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DE
Germany
Prior art keywords
pcr
pct
nucleic acid
amplification
pna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69307317T
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English (en)
Other versions
DE69307317T2 (de
Inventor
Ole Buchardt
Michael Egholm
Peter Nielsen
Rolf Berg
Christopher John Stanley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BERG ROLF HENDRIK FREDERICKSBERG DK
BUCHARDT DORTE VAERLOSE DK
EGHOLM MICHAEL FREDERIKSBERG DK
Original Assignee
BERG ROLF HENDRIK FREDERICKSBERG DK
BUCHARDT DORTE VAERLOSE DK
EGHOLM MICHAEL FREDERIKSBERG DK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by BERG ROLF HENDRIK FREDERICKSBERG DK, BUCHARDT DORTE VAERLOSE DK, EGHOLM MICHAEL FREDERIKSBERG DK filed Critical BERG ROLF HENDRIK FREDERICKSBERG DK
Publication of DE69307317D1 publication Critical patent/DE69307317D1/de
Application granted granted Critical
Publication of DE69307317T2 publication Critical patent/DE69307317T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
DE69307317T 1992-06-05 1993-06-07 Verwendung von Nuklein-säure Analoge in der Inhibition von Nuklein-säure Amplifikation Expired - Fee Related DE69307317T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929211979A GB9211979D0 (en) 1992-06-05 1992-06-05 Uses of nucleic acid analogues
PCT/EP1993/001435 WO1993025706A1 (en) 1992-06-05 1993-06-07 Use of nucleic acid analogues in the inhibition of nucleic acid amplification

Publications (2)

Publication Number Publication Date
DE69307317D1 true DE69307317D1 (de) 1997-02-20
DE69307317T2 DE69307317T2 (de) 1997-05-22

Family

ID=10716635

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69307317T Expired - Fee Related DE69307317T2 (de) 1992-06-05 1993-06-07 Verwendung von Nuklein-säure Analoge in der Inhibition von Nuklein-säure Amplifikation

Country Status (17)

Country Link
US (2) US5891625A (de)
EP (1) EP0646181B1 (de)
JP (1) JP3061064B2 (de)
KR (2) KR0140836B1 (de)
AT (1) ATE147441T1 (de)
AU (1) AU674520B2 (de)
CA (1) CA2136831C (de)
CZ (1) CZ282786B6 (de)
DE (1) DE69307317T2 (de)
DK (1) DK0646181T3 (de)
ES (1) ES2098752T3 (de)
FI (1) FI110694B (de)
GB (1) GB9211979D0 (de)
HU (1) HU219371B (de)
NO (1) NO317111B1 (de)
SK (1) SK149394A3 (de)
WO (1) WO1993025706A1 (de)

Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6020124A (en) * 1992-04-27 2000-02-01 Trustees Of Dartmouth College Detection of soluble gene sequences in biological fluids
US7825215B1 (en) 1993-04-26 2010-11-02 Peter E. Nielsen Substituted nucleic acid mimics
GB9311386D0 (en) * 1993-06-02 1993-07-21 Pna Diagnostics As Nucleic acid analogue assay procedures
EP0726965A1 (de) * 1993-11-23 1996-08-21 Ciba Corning Diagnostics Corp. Verwendung von antisense oligomere in ein verfahren für die kontrolle der kontamination in nukleinsäuren vermehrungsreaktionen
GB2284209A (en) * 1993-11-25 1995-05-31 Ole Buchardt Nucleic acid analogue-induced transcription of RNA from a double-stranded DNA template
US6133444A (en) * 1993-12-22 2000-10-17 Perseptive Biosystems, Inc. Synthons for the synthesis and deprotection of peptide nucleic acids under mild conditions
WO1995029921A1 (en) * 1994-04-29 1995-11-09 Perseptive Biosystems, Inc. Activated esters of 1-phenylpyrazolin-5-one for labelling amine-functionalized molecules
US5629152A (en) * 1994-08-01 1997-05-13 Isis Pharmaceuticals, Inc. Trisubstituted β-lactams and oligo β-lactamamides
ZA956776B (en) * 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
US6033851A (en) * 1994-12-09 2000-03-07 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
EP0813610A1 (de) * 1995-03-04 1997-12-29 Roche Diagnostics GmbH Sequenzspezifischer nachweis von nukleinsäuren
CA2219891C (en) 1995-05-05 2002-01-29 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
EP0832280A2 (de) * 1995-06-07 1998-04-01 Abbott Laboratories Maskierungsmethode von proben zur reduktion des hintergrundes in der amplifikationsreaktion
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
DE69632324T2 (de) * 1995-06-07 2005-06-16 PerSeptive Biosystems, Inc., Framingham Pna-dna-chimäre und pna-synthone zu deren herstellung
EP0870055B1 (de) 1995-10-12 2007-05-16 LANSDORP, Peter, M. Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül
WO1997014793A1 (en) * 1995-10-20 1997-04-24 Trustees Of Boston University Nucleic acid clamps
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6617422B1 (en) 1997-05-23 2003-09-09 Peter Nielsen Peptide nucleic acid monomers and oligomers
US6900012B1 (en) * 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
US7193128B2 (en) * 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7119250B2 (en) 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US6156953A (en) * 1997-06-03 2000-12-05 University Of Chicago Plant artificial chromosome compositions and methods
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
AU759915B2 (en) 1998-07-31 2003-05-01 Gen-Probe Incorporated Reversible inhibiting probes
US7981599B1 (en) 1998-07-31 2011-07-19 Boston Probes, Inc. Non-nucleic acid probes, probe sets, methods and kits pertaining to the detection of individual human chromosomes X, Y, 1, 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 16, 17, 18 and 20 as 13/21 as a pair
US6165337A (en) * 1998-12-23 2000-12-26 Shelton Scientific Manufacturing, Inc. Semi-dry electrophoresis apparatus and method
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001048242A2 (en) 1999-12-29 2001-07-05 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support
US20020015951A1 (en) * 2000-01-06 2002-02-07 Bader Joel S. Method of analyzing a nucleic acid
US6962906B2 (en) * 2000-03-14 2005-11-08 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US20040014644A1 (en) * 2000-03-14 2004-01-22 Vladimir Efimov Oligonucleotide analogues and methods of use for modulating gene expression
CA2402319A1 (en) 2000-03-14 2001-09-20 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
US6376191B1 (en) 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
US6936443B2 (en) * 2000-04-03 2005-08-30 Cytyc Corporation Detection and typing of human papillomavirus using PNA probes
DE60141205D1 (de) 2000-04-03 2010-03-18 Cytyc Corp Nachweis und typisierung des papillomavirus mittels pna-sonden
GB0021286D0 (en) * 2000-08-30 2000-10-18 Gemini Genomics Ab Identification of drug metabolic capacity
EP1349958B1 (de) 2000-09-26 2009-06-03 Boston Probes, Inc. Sonden, sondensätzen,verfahren und kits zur nachweis, identifizierung und/oder die zählung von bakterien
US6905824B2 (en) * 2000-12-15 2005-06-14 Boston Probes, Inc. Methods for determining organisms not requiring the separation of fixative or excess probe
ATE346950T1 (de) 2001-03-09 2006-12-15 Boston Probes Inc Kombinationsoligomere betreffende verfahren, kits und zusammensetzungen
JP2005524384A (ja) * 2001-09-24 2005-08-18 ボストン プローブス,インコーポレイテッド ゲノム核酸におけるランダム分布反復配列への検出可能なプローブ結合の抑制に関する方法、キットおよび組成物
EP1487858A4 (de) * 2002-03-21 2006-01-04 Boston Probes Inc Pna-oligomere, oligomersätze, verfahren und kits zum nachweis von bacillus anthracis
US20030211509A1 (en) * 2002-03-26 2003-11-13 Wiley Steven R. TNF-delta ligand and uses thereof
US7052840B2 (en) * 2002-04-03 2006-05-30 Capitol Genomix, Inc. Reversible association of nucleic acid with a carboxylated substrate
US20030211483A1 (en) * 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
WO2003100076A2 (en) * 2002-05-17 2003-12-04 Applera Corporation Pna probes, probe sets, method and kits pertaining to the determination of listeria
US20030220844A1 (en) * 2002-05-24 2003-11-27 Marnellos Georgios E. Method and system for purchasing genetic data
MXPA05000190A (es) * 2002-07-01 2005-09-30 Univ State Cleveland Metodo para detectar polinucleotidos mutados dentro de una gran poblacion de polinucleotidos de tipo silvestre.
JP4588451B2 (ja) * 2002-09-08 2010-12-01 アプライド バイオシステムズ, エルエルシー Pnaダイマーおよびpnaオリゴマー合成に関する方法、組成物およびライブラリー
DE10253966B4 (de) * 2002-11-19 2005-03-24 Clondiag Chip Technologies Gmbh Microarray-basiertes Verfahren zur Amplifikation und Detektion von Nukleinsäuren in einem kontinuierlichen Prozess
US20040259132A1 (en) * 2002-11-22 2004-12-23 Henrik Stender Peptide nucleic acid probes for analysis of pseudomonas (sensu stricto)
EP2112229A3 (de) 2002-11-25 2009-12-02 Sequenom, Inc. Verfahren zum Identifizieren des Brustkrebsrisikos und zugehörige Behandlungen
US7354715B2 (en) 2003-05-22 2008-04-08 Dow Agrosciences Llc High-throughput methods of screening DNA for deletions and other mutations
US20050221310A1 (en) * 2003-06-06 2005-10-06 Gene Ogic, Inc. Methods for enhancing gene expression analysis
WO2005003370A2 (en) * 2003-06-06 2005-01-13 Gene Logic, Inc. Methods for enhancing gene expression analysis
WO2005010209A2 (de) * 2003-07-24 2005-02-03 Qiagen Gmbh Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren
WO2005052193A2 (en) * 2003-11-19 2005-06-09 Applera Corporation Methods for determining the degradation state or concentration of nucleic acids
US8729341B2 (en) * 2004-02-23 2014-05-20 University Of Chicago Plants modified with mini-chromosomes
CA2502549C (en) 2004-04-23 2016-02-16 Becton, Dickinson And Company Use of an extraction control in a method of extracting nucleic acids
WO2005111235A2 (en) * 2004-05-04 2005-11-24 Dak Denmark A/S Methods for detecting chromosome aberrations
CA2574610A1 (en) * 2004-07-22 2006-03-02 Sequenom, Inc. Methods for identifying risk of type ii diabetes and treatments thereof
EP1836317A2 (de) * 2004-12-06 2007-09-26 BioVeris Corporation Verfahren und zusammensetzungen zum nachweis von bacillus anthracis
US7700287B2 (en) * 2005-01-28 2010-04-20 Life Technologies Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template
US20090264635A1 (en) 2005-03-25 2009-10-22 Applera Corporation Methods and compositions for depleting abundant rna transcripts
US20090246758A1 (en) 2005-06-02 2009-10-01 Advandx, Inc. Peptide nucleic acid probes for analysis of microorganisms
US8481697B2 (en) 2005-07-01 2013-07-09 Dako Denmark A/S Nucleic acid base pairs
WO2007030510A2 (en) * 2005-09-08 2007-03-15 Chromatin Inc. Plants modified with mini-chromosomes
DE102005048503B4 (de) * 2005-10-07 2012-10-18 InViTek Gesellschaft für Biotechnik & Biodesign mbH Verfahren zur Steuerung der abschnittsweisen enzymatischen Nukleinsäurevervielfältigung über inkomplette Komplementärstränge
EP2479291A1 (de) * 2005-10-27 2012-07-25 Life Technologies Corporation Nucleinsäureverstärkung unter Verwendung nichtzufälliger Primer
ATE488587T1 (de) 2005-12-06 2010-12-15 Ambion Inc Rückübertragungs-primer und verfahren zu deren entwurf
ES2345954T3 (es) * 2006-05-12 2010-10-06 Cepheid Deteccion de la union de recombinacion de adn.
EP2602321B1 (de) 2006-05-31 2017-08-23 Sequenom, Inc. Verfahren und Zusammensetzungen zur Nukleinsäureextraktion und -verstärkung aus einer Probe
US8153369B2 (en) 2006-06-05 2012-04-10 Cancer Care Ontario Assessment of risk for colorectal cancer
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
WO2008070525A1 (en) 2006-12-04 2008-06-12 Luminex Corporation Oxocarbonamide peptide nucleic acids and methods of using same
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
EP2099930B1 (de) 2006-12-13 2015-02-18 Luminex Corporation Systeme und verfahren für pcr-multiplexanalyse in echtzeit
WO2008083261A1 (en) * 2006-12-29 2008-07-10 Applera Corporation Systems and methods for detecting nucleic acids
CN101978070A (zh) * 2006-12-29 2011-02-16 应用生物系统公司 检测核酸的系统和方法
WO2008098142A2 (en) * 2007-02-08 2008-08-14 Sequenom, Inc. Nucleic acid-based tests for rhd typing, gender determination and nucleic acid quantification
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
EP2079839A4 (de) 2007-03-05 2009-11-18 Cancer Care Ontario Abschätzung des kolorektalkarzinomrisikos
US8614089B2 (en) * 2007-03-15 2013-12-24 Chromatin, Inc. Centromere sequences and minichromosomes
CN101641452B (zh) 2007-03-26 2013-10-23 塞昆纳姆股份有限公司 限制性核酸内切酶增强的多态序列检测
EP2167537A2 (de) 2007-07-03 2010-03-31 Dako Denmark A/S Zusammengestellte verfahren für die analyse und sortierung von proben
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US9605035B2 (en) 2008-01-10 2017-03-28 Research Development Foundation Vaccines and diagnostics for the ehrlichioses
AU2009223671B2 (en) 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8206926B2 (en) 2008-03-26 2012-06-26 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
CA2724638C (en) 2008-05-27 2020-02-18 Dako Denmark A/S Hybridization compositions and methods comprising a polar aprotic solvent
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) * 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
CA2743057C (en) 2008-11-07 2019-11-26 Research Development Foundation Compositions and methods for the inhibition of cripto/grp78 complex formation and signaling
CN102301005A (zh) 2008-12-17 2011-12-28 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
EP3301446B1 (de) 2009-02-11 2020-04-15 Caris MPI, Inc. Molekulare profilierung von tumoren
WO2010097655A1 (en) 2009-02-26 2010-09-02 Dako Denmark A/S Compositions and methods for rna hybridization applications
EP2408936A4 (de) * 2009-03-18 2013-01-30 Sequenom Inc Verwendung wärmestabiler endonukleasen zur herstellung von reportermolekülen
US20110045080A1 (en) * 2009-03-24 2011-02-24 William Marsh Rice University Single-Walled Carbon Nanotube/Bioactive Substance Complexes and Methods Related Thereto
CN103911428B (zh) 2009-03-27 2016-02-24 生命技术公司 用于检测等位基因变体的方法、组合物和试剂盒
EP3211095B1 (de) 2009-04-03 2019-01-02 Sequenom, Inc. Zusammensetzungen und verfahren für die herstellung von nukleinsäuren
EP2789689B1 (de) 2009-06-29 2016-04-27 Luminex Corporation Chimäre Primer mit Haarnadel-Konformationen und Verfahren zur Verwendung davon
US9096909B2 (en) 2009-07-23 2015-08-04 Chromatin, Inc. Sorghum centromere sequences and minichromosomes
US20160186266A1 (en) 2009-10-27 2016-06-30 Carislife Sciences, Inc. Molecular profiling for personalized medicine
EP2507391B1 (de) 2009-12-02 2016-08-17 Dako Denmark A/S Zusammensetzungen und verfahren zur hybridisierung ohne denaturierung
EP3660165B1 (de) 2009-12-22 2023-01-04 Sequenom, Inc. Verfahren und kits zur identifizierung von aneuploidie
WO2011087789A2 (en) 2009-12-22 2011-07-21 Becton, Dickinson And Company Methods for the detection of microorganisms
JP4960536B2 (ja) * 2010-02-25 2012-06-27 パナソニック株式会社 二本鎖dna中の標的配列を増幅する方法
WO2012095639A2 (en) 2011-01-14 2012-07-19 Genefirst Limited Methods, compositions, and kits for determing the presence/absence of a varian nucleic acid sequence
US8460872B2 (en) 2011-04-29 2013-06-11 Sequenom, Inc. Quantification of a minority nucleic acid species
EP2761028A1 (de) 2011-09-30 2014-08-06 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren mit formamid
US11118226B2 (en) 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
US20140377762A1 (en) 2011-12-19 2014-12-25 360 Genomics Ltd. Method for enriching and detection of variant target nucleic acids
US9605313B2 (en) 2012-03-02 2017-03-28 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2014033314A1 (en) 2012-09-03 2014-03-06 Uab Bioseka Antisense oligonucleotide targeting bacterial glucosyltransferases
EP2929050A1 (de) 2012-12-10 2015-10-14 AdvanDx, Inc. Verwendung von sonden zur massenspektrometrischen identifizierung von mikroorganismen oder zellen und relevanten erkrankungen von interesse
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
EP3597774A1 (de) 2013-03-13 2020-01-22 Sequenom, Inc. Primer zur analyse der dna-methylierung
KR102206573B1 (ko) 2013-03-15 2021-01-25 테출론 인코포레이티드 스타필로코커스 아우레우스 감염의 치료를 위한 안티센스 분자
WO2014144423A2 (en) 2013-03-15 2014-09-18 Techulon Inc. Antisense molecules for treatment of staphylococcus aureus infection
LT6214B (lt) 2013-10-07 2015-08-25 Uab "Bioseka" Priešprasminiai oligonukleotidai aterosklerozės ir kardiovaskulinių infekcijų prevencijai
WO2015057998A1 (en) 2013-10-16 2015-04-23 The University Of British Columbia Device for formulating particles at small volumes
EP3065706A4 (de) 2013-11-08 2017-11-29 Baylor Research Institute Nukleare lokalisierung von glp-1 zur stimulation der herzmuskelregenerierung und reversion von herzversagen
EP3117011B1 (de) 2014-03-13 2020-05-06 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
WO2016134293A1 (en) 2015-02-20 2016-08-25 Baylor College Of Medicine p63 INACTIVATION FOR THE TREATMENT OF HEART FAILURE
SG10202104058XA (en) 2015-10-14 2021-05-28 Bio Path Holdings Inc P-ethoxy nucleic acids for liposomal formulation
CN108778477B (zh) 2016-01-06 2022-02-25 不列颠哥伦比亚大学 分叉混合器及其使用和制造方法
US20190177391A1 (en) 2016-03-31 2019-06-13 Baylor Research Institute Angiopoietin-like protein 8 (angptl8)
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
KR20190053905A (ko) 2016-09-16 2019-05-20 바이오-패쓰 홀딩스 인크. 리포솜 안티센스 올리고뉴클레오타이드와의 병용 요법
EP3519578B1 (de) 2016-10-03 2021-12-22 Precision Nanosystems Inc Zusammensetzungen zur transfektion von resistenten zelltypen
CA3121170A1 (en) 2018-11-30 2020-06-04 Caris Mpi, Inc. Next-generation molecular profiling
WO2021112918A1 (en) 2019-12-02 2021-06-10 Caris Mpi, Inc. Pan-cancer platinum response predictor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
DE69131891T2 (de) * 1990-02-16 2000-06-15 Hoffmann La Roche Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
WO1992007957A1 (en) * 1990-10-31 1992-05-14 Cimino George D Methods and compositions for minimizing inhibition of nucleic acid amplification
DK51092D0 (da) * 1991-05-24 1992-04-15 Ole Buchardt Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf

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KR0140836B1 (ko) 1998-06-15
NO317111B1 (no) 2004-08-16
CZ282786B6 (cs) 1997-10-15
AU4323593A (en) 1994-01-04
HUT71931A (en) 1996-02-28
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US5891625A (en) 1999-04-06
EP0646181A1 (de) 1995-04-05
CA2136831C (en) 2002-11-05
EP0646181B1 (de) 1997-01-08
AU674520B2 (en) 1997-01-02
HU9403463D0 (en) 1995-02-28
HU219371B (en) 2001-03-28
NO944655D0 (no) 1994-12-02
WO1993025706A1 (en) 1993-12-23
DE69307317T2 (de) 1997-05-22
FI110694B (fi) 2003-03-14
KR950702252A (ko) 1995-06-19
US5972610A (en) 1999-10-26
ES2098752T3 (es) 1997-05-01
FI945725A0 (fi) 1994-12-05
SK149394A3 (en) 1996-01-10
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DK0646181T3 (da) 1997-10-13
GB9211979D0 (en) 1992-07-15

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