TW265366B - Amplification and detection of specific gene fragment of vibrio parahaemolyticus - Google Patents

Amplification and detection of specific gene fragment of vibrio parahaemolyticus

Info

Publication number
TW265366B
TW265366B TW082110632A TW82110632A TW265366B TW 265366 B TW265366 B TW 265366B TW 082110632 A TW082110632 A TW 082110632A TW 82110632 A TW82110632 A TW 82110632A TW 265366 B TW265366 B TW 265366B
Authority
TW
Taiwan
Prior art keywords
dna
primers
vibrio parahaemolyticus
gene
tdh
Prior art date
Application number
TW082110632A
Other languages
Chinese (zh)
Inventor
Jia-In Lii
Shwu-Fen Pan
Original Assignee
Jia-In Lii
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jia-In Lii filed Critical Jia-In Lii
Priority to TW082110632A priority Critical patent/TW265366B/en
Application granted granted Critical
Publication of TW265366B publication Critical patent/TW265366B/en

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Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A rapid, simple and sensitive method for amplification and detection of thermostable direct haemolysin (tdh) gene in Vibrio parahaemolyticus is provided. This method using two oligonucleotides as a primer set that hybridize specifically to the complementary DNA strands of Vibrio parahaemolyticus, flanking a unique sequence region of tdh gene, and amplifying the specific DNA fragment. The assay including the following steps: (A) hybridization of the primers to the target gene sequence in a bacterial specimen and polymerization of the primers with dNTPs (dATP, dCTP, dGTP and dTTP), DNA polymerase and enzyme buffer to produce a double standed DNA; (B) denaturation of the products thermally to make single strand of DNA and then hybridization of the primers to the target DNA and polymerization of the primers, the PCR is performed in 25 cycles to amplify an specific nucleotide fragment; (C) determination of the presence or absence of the specific nucleotide fragment in the specimen, confirmation of the tdh gene carrying Vibrio parahaemolyticus.
TW082110632A 1993-12-15 1993-12-15 Amplification and detection of specific gene fragment of vibrio parahaemolyticus TW265366B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
TW082110632A TW265366B (en) 1993-12-15 1993-12-15 Amplification and detection of specific gene fragment of vibrio parahaemolyticus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
TW082110632A TW265366B (en) 1993-12-15 1993-12-15 Amplification and detection of specific gene fragment of vibrio parahaemolyticus

Publications (1)

Publication Number Publication Date
TW265366B true TW265366B (en) 1995-12-11

Family

ID=51402117

Family Applications (1)

Application Number Title Priority Date Filing Date
TW082110632A TW265366B (en) 1993-12-15 1993-12-15 Amplification and detection of specific gene fragment of vibrio parahaemolyticus

Country Status (1)

Country Link
TW (1) TW265366B (en)

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