DK1572959T3 - Hybridpolymerase-fremgangsmåder og -sammensætninger - Google Patents
Hybridpolymerase-fremgangsmåder og -sammensætningerInfo
- Publication number
- DK1572959T3 DK1572959T3 DK03771830.1T DK03771830T DK1572959T3 DK 1572959 T3 DK1572959 T3 DK 1572959T3 DK 03771830 T DK03771830 T DK 03771830T DK 1572959 T3 DK1572959 T3 DK 1572959T3
- Authority
- DK
- Denmark
- Prior art keywords
- compositions
- methods
- hybrid polymerase
- polymerase methods
- proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39868702P | 2002-07-25 | 2002-07-25 | |
| US46378103P | 2003-04-17 | 2003-04-17 | |
| US48328703P | 2003-06-27 | 2003-06-27 | |
| PCT/US2003/023278 WO2004011605A2 (en) | 2002-07-25 | 2003-07-25 | Hybrid protein methods and compositions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1572959T3 true DK1572959T3 (da) | 2011-02-07 |
Family
ID=31192091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK03771830.1T DK1572959T3 (da) | 2002-07-25 | 2003-07-25 | Hybridpolymerase-fremgangsmåder og -sammensætninger |
Country Status (8)
| Country | Link |
|---|---|
| EP (2) | EP2264155A1 (da) |
| JP (1) | JP4575160B2 (da) |
| AT (1) | ATE486931T1 (da) |
| AU (1) | AU2003256794B2 (da) |
| CA (1) | CA2518588A1 (da) |
| DE (1) | DE60334839D1 (da) |
| DK (1) | DK1572959T3 (da) |
| WO (1) | WO2004011605A2 (da) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9040276B2 (en) | 2005-07-15 | 2015-05-26 | Agilent Technologies, Inc. | DNA binding protein-polymerase chimeras |
| JP5818688B2 (ja) | 2009-01-08 | 2015-11-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 核酸増幅反応の効率を改善するための方法および組成物 |
| US9778188B2 (en) | 2009-03-11 | 2017-10-03 | Industrial Technology Research Institute | Apparatus and method for detection and discrimination molecular object |
| JP2013505016A (ja) * | 2009-09-16 | 2013-02-14 | マッシー ユニヴァーシティー | 融合ポリペプチドおよびその使用 |
| US9482615B2 (en) | 2010-03-15 | 2016-11-01 | Industrial Technology Research Institute | Single-molecule detection system and methods |
| US9670243B2 (en) | 2010-06-02 | 2017-06-06 | Industrial Technology Research Institute | Compositions and methods for sequencing nucleic acids |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| WO2012138417A1 (en) | 2011-04-08 | 2012-10-11 | Bio-Rad Laboratories, Inc. | Sso7-polymerase conjugates with decreased non-specific activity |
| US8916352B2 (en) | 2011-04-08 | 2014-12-23 | Bio-Rad Laboratories, Inc. | PCR reaction mixtures with decreased non-specific activity |
| US8470573B2 (en) * | 2011-06-21 | 2013-06-25 | Bio-Rad Laboratories, Inc. | Hybrid polymerases having the ability to produce long amplicons |
| EP3620533B1 (en) | 2011-09-06 | 2023-01-18 | Gen-Probe Incorporated | Closed nucleic acid structures |
| EP2753714B1 (en) | 2011-09-06 | 2017-04-12 | Gen-Probe Incorporated | Circularized templates for sequencing |
| CN107250382A (zh) | 2015-02-17 | 2017-10-13 | 生物辐射实验室股份有限公司 | 使用分裂循环扩增的小核酸定量 |
| US10626383B2 (en) | 2016-01-15 | 2020-04-21 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| EP3645710A1 (en) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US852444A (en) | 1906-12-05 | 1907-05-07 | Carl J Mellin | Locomotive. |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US5352778A (en) * | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
| US5489523A (en) | 1990-12-03 | 1996-02-06 | Stratagene | Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I |
| US5948663A (en) | 1990-12-03 | 1999-09-07 | Stratagene | Purified thermostable pyrococcus furiosus DNA polymerase I |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US6150111A (en) | 1999-03-15 | 2000-11-21 | The Trustees Of The University Of Pennsylvania | Methods and kits for recombining nucleic acids |
| ATE395420T1 (de) * | 2000-02-17 | 2008-05-15 | Qiagen Gmbh | Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen |
| US6627424B1 (en) * | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
-
2003
- 2003-07-25 EP EP10010578A patent/EP2264155A1/en not_active Withdrawn
- 2003-07-25 WO PCT/US2003/023278 patent/WO2004011605A2/en active Search and Examination
- 2003-07-25 CA CA002518588A patent/CA2518588A1/en not_active Abandoned
- 2003-07-25 EP EP03771830A patent/EP1572959B1/en not_active Expired - Lifetime
- 2003-07-25 AT AT03771830T patent/ATE486931T1/de active
- 2003-07-25 AU AU2003256794A patent/AU2003256794B2/en not_active Ceased
- 2003-07-25 JP JP2004524808A patent/JP4575160B2/ja not_active Expired - Fee Related
- 2003-07-25 DK DK03771830.1T patent/DK1572959T3/da active
- 2003-07-25 DE DE60334839T patent/DE60334839D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004011605A2 (en) | 2004-02-05 |
| WO2004011605A3 (en) | 2006-04-20 |
| DE60334839D1 (de) | 2010-12-16 |
| EP2264155A1 (en) | 2010-12-22 |
| AU2003256794B2 (en) | 2008-07-31 |
| EP1572959A4 (en) | 2007-05-02 |
| JP4575160B2 (ja) | 2010-11-04 |
| CA2518588A1 (en) | 2004-02-05 |
| EP1572959A2 (en) | 2005-09-14 |
| ATE486931T1 (de) | 2010-11-15 |
| EP1572959B1 (en) | 2010-11-03 |
| JP2006502706A (ja) | 2006-01-26 |
| AU2003256794A1 (en) | 2004-02-16 |
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