DK3303614T3 - Forbedret anvendelse af overfladeprimere i klynger - Google Patents
Forbedret anvendelse af overfladeprimere i klynger Download PDFInfo
- Publication number
- DK3303614T3 DK3303614T3 DK16726415.9T DK16726415T DK3303614T3 DK 3303614 T3 DK3303614 T3 DK 3303614T3 DK 16726415 T DK16726415 T DK 16726415T DK 3303614 T3 DK3303614 T3 DK 3303614T3
- Authority
- DK
- Denmark
- Prior art keywords
- clusters
- improved application
- surface primers
- primers
- improved
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/10—Characterised by chemical treatment
- C12Q2523/113—Denaturating agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2523/00—Reactions characterised by treatment of reaction samples
- C12Q2523/30—Characterised by physical treatment
- C12Q2523/305—Denaturation or renaturation by physical action
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/131—Modifications characterised by incorporating a restriction site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562168602P | 2015-05-29 | 2015-05-29 | |
| PCT/GB2016/051574 WO2016193695A1 (en) | 2015-05-29 | 2016-05-27 | Enhanced utilization of surface primers in clusters |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3303614T3 true DK3303614T3 (da) | 2020-05-18 |
Family
ID=56096664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16726415.9T DK3303614T3 (da) | 2015-05-29 | 2016-05-27 | Forbedret anvendelse af overfladeprimere i klynger |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10590464B2 (da) |
| EP (1) | EP3303614B1 (da) |
| JP (1) | JP6609641B2 (da) |
| KR (1) | KR102054571B1 (da) |
| CN (1) | CN107849598B (da) |
| AU (1) | AU2016269785B2 (da) |
| CA (1) | CA2985545C (da) |
| DK (1) | DK3303614T3 (da) |
| ES (1) | ES2789349T3 (da) |
| HK (1) | HK1246366A1 (da) |
| WO (1) | WO2016193695A1 (da) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201704754D0 (en) * | 2017-01-05 | 2017-05-10 | Illumina Inc | Kinetic exclusion amplification of nucleic acid libraries |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11236388B1 (en) | 2021-06-17 | 2022-02-01 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| CA2277159A1 (en) | 1997-01-08 | 1998-07-16 | Wolfgang Pieken | Bioconjugation of macromolecules |
| DE69837913T2 (de) | 1997-04-01 | 2008-02-07 | Solexa Ltd., Saffron Walden | Verfahren zur vervielfältigung von nukleinsäure |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US7427678B2 (en) | 1998-01-08 | 2008-09-23 | Sigma-Aldrich Co. | Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| CN1500150A (zh) | 1999-12-29 | 2004-05-26 | ��Ĭ���� | 在固相载体上扩增和检测多个多核苷酸的方法 |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US20030064366A1 (en) | 2000-07-07 | 2003-04-03 | Susan Hardin | Real-time sequence determination |
| EP1354064A2 (en) | 2000-12-01 | 2003-10-22 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| CA2442939A1 (en) * | 2001-04-02 | 2002-10-17 | Point-2-Point Genomics Ltd. | Polynucleotide analysis using combinatorial pcr |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| CA2487424C (en) | 2002-05-30 | 2011-01-04 | The Scripps Research Institute | Copper-catalysed ligation of azides and acetylenes |
| EP2607369B1 (en) | 2002-08-23 | 2015-09-23 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
| AU2003272438B2 (en) | 2002-09-20 | 2009-04-02 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7259258B2 (en) | 2003-12-17 | 2007-08-21 | Illumina, Inc. | Methods of attaching biological compounds to solid supports using triazine |
| EP2789383B1 (en) | 2004-01-07 | 2023-05-03 | Illumina Cambridge Limited | Molecular arrays |
| EP1790202A4 (en) | 2004-09-17 | 2013-02-20 | Pacific Biosciences California | APPARATUS AND METHOD FOR ANALYZING MOLECULES |
| GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
| WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
| WO2006120433A1 (en) | 2005-05-10 | 2006-11-16 | Solexa Limited | Improved polymerases |
| GB0514910D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Method for sequencing a polynucleotide template |
| GB0514936D0 (en) * | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| DK1987159T4 (da) | 2006-02-08 | 2020-11-16 | Illumina Cambridge Ltd | Fremgangsmåde til sekventering af en polynukleotidtemplate |
| US8241573B2 (en) | 2006-03-31 | 2012-08-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| CA2666517A1 (en) | 2006-10-23 | 2008-05-02 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP2639578B1 (en) | 2006-12-14 | 2016-09-14 | Life Technologies Corporation | Apparatus for measuring analytes using large scale fet arrays |
| JP2009000070A (ja) * | 2007-06-22 | 2009-01-08 | Hitachi Ltd | 大規模並列核酸分析方法 |
| US8198028B2 (en) * | 2008-07-02 | 2012-06-12 | Illumina Cambridge Limited | Using populations of beads for the fabrication of arrays on surfaces |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9309566B2 (en) * | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| EP3564392B1 (en) * | 2010-12-17 | 2021-11-24 | Life Technologies Corporation | Methods for nucleic acid amplification |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| US8778848B2 (en) | 2011-06-09 | 2014-07-15 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
| CA2839351C (en) * | 2011-07-13 | 2015-11-24 | Nordion (Canada) Inc. | Radiolabelled rotenone derivatives and their use in spect imaging |
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| CN114854832A (zh) * | 2012-04-19 | 2022-08-05 | 生命技术公司 | 核酸扩增 |
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| US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
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| CN103388024B (zh) * | 2013-07-04 | 2015-06-17 | 徐州医学院 | 一种基于桥式pcr检测dna羟甲基化的方法 |
-
2016
- 2016-05-27 ES ES16726415T patent/ES2789349T3/es active Active
- 2016-05-27 KR KR1020177037802A patent/KR102054571B1/ko active IP Right Grant
- 2016-05-27 WO PCT/GB2016/051574 patent/WO2016193695A1/en active Application Filing
- 2016-05-27 US US15/576,211 patent/US10590464B2/en active Active
- 2016-05-27 AU AU2016269785A patent/AU2016269785B2/en active Active
- 2016-05-27 CA CA2985545A patent/CA2985545C/en active Active
- 2016-05-27 EP EP16726415.9A patent/EP3303614B1/en active Active
- 2016-05-27 JP JP2017558661A patent/JP6609641B2/ja active Active
- 2016-05-27 CN CN201680030000.3A patent/CN107849598B/zh active Active
- 2016-05-27 DK DK16726415.9T patent/DK3303614T3/da active
-
2018
- 2018-05-08 HK HK18105926.8A patent/HK1246366A1/zh unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20180142281A1 (en) | 2018-05-24 |
| ES2789349T3 (es) | 2020-10-26 |
| AU2016269785A1 (en) | 2017-11-30 |
| EP3303614A1 (en) | 2018-04-11 |
| US10590464B2 (en) | 2020-03-17 |
| CN107849598B (zh) | 2021-12-14 |
| JP2018516074A (ja) | 2018-06-21 |
| KR102054571B1 (ko) | 2019-12-10 |
| KR20180039585A (ko) | 2018-04-18 |
| JP6609641B2 (ja) | 2019-11-20 |
| CA2985545A1 (en) | 2016-12-08 |
| CN107849598A (zh) | 2018-03-27 |
| EP3303614B1 (en) | 2020-03-11 |
| HK1246366A1 (zh) | 2018-09-07 |
| AU2016269785B2 (en) | 2019-02-28 |
| CA2985545C (en) | 2021-02-09 |
| WO2016193695A1 (en) | 2016-12-08 |
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