RU98105786A - Модифицированные праймеры - Google Patents
Модифицированные праймерыInfo
- Publication number
- RU98105786A RU98105786A RU98105786/04A RU98105786A RU98105786A RU 98105786 A RU98105786 A RU 98105786A RU 98105786/04 A RU98105786/04 A RU 98105786/04A RU 98105786 A RU98105786 A RU 98105786A RU 98105786 A RU98105786 A RU 98105786A
- Authority
- RU
- Russia
- Prior art keywords
- group
- denotes
- oligonucleotide
- oligonucleotide according
- paragraphs
- Prior art date
Links
- 229920000272 Oligonucleotide Polymers 0.000 claims 10
- -1 2-naphthylmethyl group Chemical group 0.000 claims 5
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 2
- 150000001412 amines Chemical class 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 claims 2
- 125000001624 naphthyl group Chemical group 0.000 claims 2
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 150000007523 nucleic acids Chemical group 0.000 claims 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 2
- 125000000008 (C1-C10) alkyl group Chemical group 0.000 claims 1
- 125000004178 (C1-C4) alkyl group Chemical group 0.000 claims 1
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 claims 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N 289-95-2 Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims 1
- GAFBGRBPYCNUCH-UHFFFAOYSA-N 4-Methylbenzyl radical Chemical group [CH2]C1=CC=C(C)C=C1 GAFBGRBPYCNUCH-UHFFFAOYSA-N 0.000 claims 1
- OIRDTQYFTABQOQ-GAWUUDPSSA-N 9-β-D-XYLOFURANOSYL-ADENINE Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@H](O)[C@H]1O OIRDTQYFTABQOQ-GAWUUDPSSA-N 0.000 claims 1
- OIRDTQYFTABQOQ-SXVXDFOESA-N Adenosine Natural products Nc1ncnc2c1ncn2[C@@H]3O[C@@H](CO)[C@H](O)[C@@H]3O OIRDTQYFTABQOQ-SXVXDFOESA-N 0.000 claims 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 1
- KDCGOANMDULRCW-UHFFFAOYSA-N Purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims 1
- 229960005305 adenosine Drugs 0.000 claims 1
- 125000003545 alkoxy group Chemical group 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 125000004435 hydrogen atoms Chemical class [H]* 0.000 claims 1
- 239000003607 modifier Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 125000000951 phenoxy group Chemical group [H]C1=C([H])C([H])=C(O*)C([H])=C1[H] 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
Claims (11)
1. Олигонуклеотид, имеющий общее строение
где S1 обозначает первую нуклеотидную последовательность, имеющую длину от приблизительно 5 до приблизительно 50 нуклеотидов;
S2 обозначает вторую последовательность, имеющую длину от одного до трех нуклеотидов;
N обозначает нуклеотид, который содержит пуриновое или пиримидиновое основание, в состав которого входит экзоциклический амин;
R обозначает группу-модификатор, причем R ковалентно связан с N через экзоциклический амин и имеет следующую структуру:
где R1 и R2 независимо друг от друга обозначают водород, С1-С10алкильную группу, алкоксигруппу, фенильную группу, феноксигруппу, замещенную фенильную группу, нафтильную группу или замещенную нафтильную группу.
где S1 обозначает первую нуклеотидную последовательность, имеющую длину от приблизительно 5 до приблизительно 50 нуклеотидов;
S2 обозначает вторую последовательность, имеющую длину от одного до трех нуклеотидов;
N обозначает нуклеотид, который содержит пуриновое или пиримидиновое основание, в состав которого входит экзоциклический амин;
R обозначает группу-модификатор, причем R ковалентно связан с N через экзоциклический амин и имеет следующую структуру:
где R1 и R2 независимо друг от друга обозначают водород, С1-С10алкильную группу, алкоксигруппу, фенильную группу, феноксигруппу, замещенную фенильную группу, нафтильную группу или замещенную нафтильную группу.
2. Олигонуклеотид по п. 1, где R обозначает 2-нафтилметильную группу, бензильную группу или замещенную бензильную группу.
3. Олигонуклеотид по п. 1 или 2, где R обозначает замещенную бензильную группу, имеющую следующую структуру:
где R3 обозначает разветвленную или линейную C1-С6алкильную группу, метоксигруппу или нитрогруппу.
где R3 обозначает разветвленную или линейную C1-С6алкильную группу, метоксигруппу или нитрогруппу.
4. Олигонуклеотид по любому из пп. 1-3, где R3 обозначает разветвленную или линейную C1-С4алкильную группу, метоксигруппу или нитрогруппу.
5. Олигонуклеотид по п. 3 или 4, где R3 присоединен в пара-положении.
6. Олигонуклеотид по любому из пп. 1-5, где N обозначает аденозин.
7. Олигонуклеотид по любому из пп. 1-6, где R выбирают из группы, включающей бензил, пара-метилбензил, пара-трет-бутилбензил, пара-метоксибензил, орто-нитробензил и 2-нафтилметил.
8. Способ амплификации последовательности нуклеиновой кислоты-мишени, включающий проведение реакции амплификации с использованием по крайней мере одного олигонуклеотида по любому из пп. 1-7.
9. Способ по п. 8, где способ представляет собой полимеразную цепную реакцию.
10. Набор для проведения реакции амплификации нуклеиновой кислоты, включающий олигонуклеотид по любому из пп. 1-7.
11. Новые олигонуклеотиды, способы и наборы, в основном представленные в настоящем описании.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4112797P | 1997-03-20 | 1997-03-20 | |
| US60/041127 | 1997-03-20 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU98105786A true RU98105786A (ru) | 1999-12-20 |
| RU2159248C2 RU2159248C2 (ru) | 2000-11-20 |
Family
ID=21914898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU98105786/04A RU2159248C2 (ru) | 1997-03-20 | 1998-03-19 | Олигонуклеотид, способ амплификации нуклеиновой кислоты-мишени и набор для проведения реакции амплификации |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US6001611A (ru) |
| EP (1) | EP0866071B1 (ru) |
| JP (1) | JP2966389B2 (ru) |
| KR (1) | KR100292997B1 (ru) |
| CN (1) | CN1065873C (ru) |
| AT (1) | ATE280177T1 (ru) |
| AU (1) | AU712448B2 (ru) |
| BR (1) | BR9801878B1 (ru) |
| CA (1) | CA2229766C (ru) |
| CZ (1) | CZ291525B6 (ru) |
| DE (1) | DE69827060T2 (ru) |
| DK (1) | DK0866071T3 (ru) |
| ES (1) | ES2230631T3 (ru) |
| HK (1) | HK1012192A1 (ru) |
| HU (1) | HU223669B1 (ru) |
| IL (1) | IL123694A (ru) |
| NO (1) | NO322136B1 (ru) |
| PL (1) | PL190142B1 (ru) |
| RU (1) | RU2159248C2 (ru) |
| TW (1) | TW567188B (ru) |
| UA (1) | UA49843C2 (ru) |
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