RU93004859A - Полинуклеотиды, способы получения полинуклеотидов, способы гибридизации нуклеиновых кислот - Google Patents
Полинуклеотиды, способы получения полинуклеотидов, способы гибридизации нуклеиновых кислотInfo
- Publication number
- RU93004859A RU93004859A RU93004859/04A RU93004859A RU93004859A RU 93004859 A RU93004859 A RU 93004859A RU 93004859/04 A RU93004859/04 A RU 93004859/04A RU 93004859 A RU93004859 A RU 93004859A RU 93004859 A RU93004859 A RU 93004859A
- Authority
- RU
- Russia
- Prior art keywords
- polinucleotides
- methods
- nucleotides
- segment
- nucleic acid
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title claims 3
- 150000007523 nucleic acids Chemical class 0.000 title claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 5
- 229920000272 Oligonucleotide Polymers 0.000 claims 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 238000004166 bioassay Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000007899 nucleic acid hybridization Methods 0.000 claims 1
- 239000002751 oligonucleotide probe Substances 0.000 claims 1
- 229920000023 polynucleotide Polymers 0.000 claims 1
- 239000002157 polynucleotide Substances 0.000 claims 1
Claims (1)
- В гибридизационных анализах нуклеиновой кислоты в качестве амплификационных мультимеров применяются гребневидно-разветвленные полинуклеотиды следующей формулы:
где: S - первый спейсерный сегмент из, по крайней мере, 15 нуклеотидов; X - модифицированный нуклеотид, предоставляющий место для ветвления; S' - спейсерный сегмент места ветвления, состоящий, примерно, из 0-15 нуклеотидов; m - целое число, равное или больше 15; R - молекула расщепляемого линкера; n - 0 или 1; S" - второй спейсерный сегмент, состоящий, примерно, из 0-10 нуклеотидов; А - сегмент, способный специфически образовывать гибрид с анализируемой нуклеиновой кислотой или с нуклеиновой кислотой, связанной с анализируемым материалом; S''' - третий спейсерный сегмент, состоящий из 0-10 нуклеотидов; Е - область олигонуклеотидного удлинения, состоящая из 5-10 нуклеотидов, и L - сегмент, содержащий 2 - 10 повторений последовательности, способной специфически образовывать гибрид с меченым олигонуклеотидным зондом.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55889790A | 1990-07-27 | 1990-07-27 | |
| US07/558,897 | 1990-07-27 | ||
| PCT/US1991/005363 WO1992002526A1 (en) | 1990-07-27 | 1991-07-29 | Large comb-type branched polynucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU93004859A true RU93004859A (ru) | 1997-01-20 |
| RU2142467C1 RU2142467C1 (ru) | 1999-12-10 |
Family
ID=24231439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU93004859A RU2142467C1 (ru) | 1990-07-27 | 1991-07-29 | Полинуклеотиды, способ их получения, гибридизационный анализ нуклеиновой кислоты |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0541693B1 (ru) |
| JP (1) | JP2641322B2 (ru) |
| AT (1) | ATE174339T1 (ru) |
| AU (1) | AU8412991A (ru) |
| CA (1) | CA2087961A1 (ru) |
| DE (1) | DE69130612T2 (ru) |
| IE (1) | IE912662A1 (ru) |
| PL (1) | PL169801B1 (ru) |
| PT (1) | PT98489B (ru) |
| RU (1) | RU2142467C1 (ru) |
| WO (1) | WO1992002526A1 (ru) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07502658A (ja) * | 1991-12-23 | 1995-03-23 | バイエル コーポレイション | 溶液相サンドイッチハイブリダイゼーションアッセイに用いるためのｈａｖプローブ |
| ATE199158T1 (de) * | 1991-12-23 | 2001-02-15 | Bayer Ag | Hbv amplifizierungssonden zur verwendung in hybridisierungssandwichassay in der lösungsphase |
| US5561043A (en) * | 1994-01-31 | 1996-10-01 | Trustees Of Boston University | Self-assembling multimeric nucleic acid constructs |
| US5580731A (en) * | 1994-08-25 | 1996-12-03 | Chiron Corporation | N-4 modified pyrimidine deoxynucleotides and oligonucleotide probes synthesized therewith |
| EP0862651A2 (en) * | 1995-10-16 | 1998-09-09 | Chiron Corporation | Method of screening for factors that modulate gene expression |
| NZ508366A (en) | 1998-05-01 | 2004-03-26 | Chiron Corp | Neisseria meningitidis antigens and compositions |
| US6203989B1 (en) | 1998-09-30 | 2001-03-20 | Affymetrix, Inc. | Methods and compositions for amplifying detectable signals in specific binding assays |
| WO2000068434A2 (en) * | 1999-05-07 | 2000-11-16 | Yale University | Multiple tag analysis |
| US7700359B2 (en) | 2000-06-02 | 2010-04-20 | Novartis Vaccines And Diagnostics, Inc. | Gene products differentially expressed in cancerous cells |
| EP1370684B1 (en) | 2000-06-15 | 2008-05-28 | Novartis Vaccines and Diagnostics, Inc. | Polynucleotides related to colon cancer |
| WO2002046477A2 (en) | 2000-12-07 | 2002-06-13 | Chiron Corporation | Endogenous retroviruses up-regulated in prostate cancer |
| DE60326931D1 (de) | 2002-01-08 | 2009-05-14 | Novartis Vaccines & Diagnostic | In kanzerösen mammazellen differentiell exprimierte genprodukte und ihre verwendungsverfahren |
| AU2003218350A1 (en) | 2002-03-21 | 2003-10-08 | Sagres Discovery, Inc. | Novel compositions and methods in cancer |
| EP1532161B1 (en) | 2002-06-13 | 2012-02-15 | Novartis Vaccines and Diagnostics, Inc. | Vectors for expression of hml-2 polypeptides |
| US7767387B2 (en) | 2003-06-13 | 2010-08-03 | Sagres Discovery, Inc. | Therapeutic targets in cancer |
| CA2516138A1 (en) | 2003-02-14 | 2004-09-02 | Sagres Discovery, Inc. | Therapeutic gpcr targets in cancer |
| US20040170982A1 (en) | 2003-02-14 | 2004-09-02 | Morris David W. | Novel therapeutic targets in cancer |
| US20060024677A1 (en) | 2004-07-20 | 2006-02-02 | Morris David W | Novel therapeutic targets in cancer |
| AU2006235276A1 (en) | 2005-04-07 | 2006-10-19 | Novartis Vaccines And Diagnostics Inc. | CACNA1E in cancer diagnosis, detection and treatment |
| WO2006110585A2 (en) | 2005-04-07 | 2006-10-19 | Novartis Vaccines And Diagnostics Inc. | Cancer-related genes (prlr) |
| JP2008043332A (ja) * | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
| RU2667524C2 (ru) * | 2011-04-21 | 2018-09-21 | Ионис Фармасьютикалз, Инк. | Модулирование экспрессии вируса гепатита b (hbv) |
| EP2984074A1 (en) | 2012-12-14 | 2016-02-17 | Basf Se | Malononitrile compounds for controlling animal pests |
| WO2017182981A1 (en) | 2016-04-20 | 2017-10-26 | Washington University | Ppar agonist or lxr agonist for use in the treatment of systemic lupus erythematosus by modulation of lap activity |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4626501A (en) * | 1983-09-02 | 1986-12-02 | Integrated Genetics, Inc. | Labeled DNA |
| US4843122A (en) * | 1984-01-30 | 1989-06-27 | Enzo Biochem, Inc. | Detectable molecules, method of preparation and use |
| US4755458A (en) * | 1984-08-30 | 1988-07-05 | Enzo Biochem, Inc. | Composition and method for the detection of the presence of a polynucleotide sequence of interest |
| US4910300A (en) * | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| CA1339351C (en) * | 1987-10-15 | 1997-08-26 | Michael S. Urdea | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
-
1991
- 1991-07-29 EP EP91915000A patent/EP0541693B1/en not_active Expired - Lifetime
- 1991-07-29 RU RU93004859A patent/RU2142467C1/ru active
- 1991-07-29 WO PCT/US1991/005363 patent/WO1992002526A1/en active IP Right Grant
- 1991-07-29 IE IE266291A patent/IE912662A1/en unknown
- 1991-07-29 AT AT91915000T patent/ATE174339T1/de not_active IP Right Cessation
- 1991-07-29 PT PT98489A patent/PT98489B/pt not_active IP Right Cessation
- 1991-07-29 JP JP3514126A patent/JP2641322B2/ja not_active Expired - Fee Related
- 1991-07-29 DE DE69130612T patent/DE69130612T2/de not_active Expired - Lifetime
- 1991-07-29 CA CA002087961A patent/CA2087961A1/en not_active Abandoned
- 1991-07-29 AU AU84129/91A patent/AU8412991A/en not_active Abandoned
- 1991-07-29 PL PL91297659A patent/PL169801B1/pl not_active IP Right Cessation
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