ES2130177T3 - Tratamiento de poblaciones de celulas. - Google Patents

Tratamiento de poblaciones de celulas.

Info

Publication number
ES2130177T3
ES2130177T3 ES92916742T ES92916742T ES2130177T3 ES 2130177 T3 ES2130177 T3 ES 2130177T3 ES 92916742 T ES92916742 T ES 92916742T ES 92916742 T ES92916742 T ES 92916742T ES 2130177 T3 ES2130177 T3 ES 2130177T3
Authority
ES
Spain
Prior art keywords
pct
copies
sec
treatment
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92916742T
Other languages
English (en)
Inventor
Michael Jim Embleton
Guy Gorochov
Peter Thomas Jones
Gregory Paul Winter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB919117352A external-priority patent/GB9117352D0/en
Priority claimed from GB929212419A external-priority patent/GB9212419D0/en
Application filed by Medical Research Council filed Critical Medical Research Council
Application granted granted Critical
Publication of ES2130177T3 publication Critical patent/ES2130177T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Abstract

SE DESCRIBE UN METODO DE TRATAMIENTO DE UNA POBLACION HETEROGENEA DE CELULAS PARA ENLAZAR COPIAS JUNTAMENTE DE DOS O MAS SECUENCIAS DE ACIDO NUCLEICO, PARTIENDO DE, AL MENOS, ALGUNAS DE LAS CELULAS, SIENDO LA DISPOSICION TAL QUE LAS COPIAS DE LAS SECUENCIAS DEL DNA DESDE UNA CELULA INDIVIDUAL SE UNEN PREFERENCIALMENTE EN LA PROXIMIDAD DEL ACIDO NUCLEICO DESDE DONDE PROVIENEN LAS COPIAS. TAMBIEN SE DESCRIBEN PROTEINAS RECOMBINANTES MEDIANTE EL METODO DE LA INVENCION Y EQUIPOS PARA REALIZAR ESTE METODO.
ES92916742T 1991-08-10 1992-08-10 Tratamiento de poblaciones de celulas. Expired - Lifetime ES2130177T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB919117352A GB9117352D0 (en) 1991-08-10 1991-08-10 Recombinant dna method
GB929212419A GB9212419D0 (en) 1992-06-11 1992-06-11 Recombinant dna method

Publications (1)

Publication Number Publication Date
ES2130177T3 true ES2130177T3 (es) 1999-07-01

Family

ID=26299394

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92916742T Expired - Lifetime ES2130177T3 (es) 1991-08-10 1992-08-10 Tratamiento de poblaciones de celulas.

Country Status (11)

Country Link
US (1) US5830663A (es)
EP (1) EP0597960B1 (es)
JP (1) JPH06509473A (es)
AT (1) ATE175997T1 (es)
AU (1) AU665365B2 (es)
CA (1) CA2114950A1 (es)
DE (1) DE69228247T2 (es)
DK (1) DK0597960T3 (es)
ES (1) ES2130177T3 (es)
GR (1) GR3029913T3 (es)
WO (1) WO1993003151A1 (es)

Families Citing this family (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830713A (en) * 1986-11-04 1998-11-03 Protein Polymer Technologies, Inc. Methods for preparing synthetic repetitive DNA
ATE463573T1 (de) 1991-12-02 2010-04-15 Medimmune Ltd Herstellung von autoantikörpern auf phagenoberflächen ausgehend von antikörpersegmentbibliotheken
GB9216983D0 (en) * 1992-08-11 1992-09-23 Unilever Plc Polypeptide production
GB9324807D0 (en) * 1993-12-03 1994-01-19 Cancer Res Campaign Tech Tumour antibody
ATE300610T1 (de) * 1994-01-31 2005-08-15 Univ Boston Bibliotheken aus polyklonalen antikörpern
GB9401815D0 (en) * 1994-01-31 1994-03-23 Ray Ronnie A A new method for combined intracellular reverse transciption and amplification
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
ES2563643T3 (es) * 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
EP1019496B1 (en) 1997-07-07 2004-09-29 Medical Research Council In vitro sorting method
AU760678B2 (en) * 1998-05-08 2003-05-22 Stichting Sanquin Bloedvoorziening Method for diagnosis and treatment of haemophilia a patients with an inhibitor
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
ATE519840T1 (de) 1998-12-16 2011-08-15 Novartis Vaccines & Diagnostic Menschliche cyclin-abhängige kinase (hpnqalre)
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
JP2002539800A (ja) * 1999-03-24 2002-11-26 ジーン セラピー システムズ インコーポレーテッド 転写活性のあるｄｎａ断片を製造する方法
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
WO2001062908A2 (en) * 2000-02-22 2001-08-30 Ahuva Nissim Chimeric and tcr phage display libraries, chimeric and tcr reagents and methods of use thereof
US6211164B1 (en) * 2000-03-10 2001-04-03 Abbott Laboratories Antisense oligonucleotides of the human chk1 gene and uses thereof
DK2199393T3 (da) * 2000-04-17 2012-11-26 Dyax Corp Hidtil ukendte fremgangsmåder til opbygning af biblioteker over genetiske pakker, der kollektivt viser medlemmerne af en forskelligartet familie af peptider, polypeptider eller proteiner
US8288322B2 (en) 2000-04-17 2012-10-16 Dyax Corp. Methods of constructing libraries comprising displayed and/or expressed members of a diverse family of peptides, polypeptides or proteins and the novel libraries
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
WO2002023163A1 (en) * 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
CA2429544C (en) * 2000-11-17 2010-10-19 University Of Rochester In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
PT2316940E (pt) 2000-12-18 2013-10-16 Dyax Corp Bibliotecas orientadas de pacotes genéticos
US20030104402A1 (en) * 2001-01-23 2003-06-05 University Of Rochester Methods of producing or identifying intrabodies in eukaryotic cells
WO2002070678A2 (en) 2001-02-05 2002-09-12 Bayer Aktiengesellschaft Regulation of human serine/threonine protein kinase
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
EP1365034A3 (en) 2002-05-21 2004-02-18 Bayer HealthCare AG Methods and compositions for the prediction, diagnosis, prognosis, prevention and treatment of malignant neoplasia
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
EP1587940A4 (en) * 2002-12-20 2006-06-07 Caliper Life Sciences Inc SINGLE MOLECULAR AMPLIFICATION AND DNA PROOF
ES2396245T3 (es) * 2003-01-29 2013-02-20 454 Life Sciences Corporation Método de amplificación y secuenciamiento de ácidos nucleicos
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
DK1644493T3 (da) 2003-06-27 2010-09-13 Axikin Pharmaceuticals Inc Regulering af en kinase, 'reguleret i COPD kinase' (RC-kinase)
WO2005010145A2 (en) 2003-07-05 2005-02-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
CA2536565A1 (en) * 2003-09-10 2005-05-12 Althea Technologies, Inc. Expression profiling using microarrays
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
KR101135134B1 (ko) * 2003-09-18 2012-04-13 심포젠 에이/에스 관심있는 서열을 결합시키는 방법
EP1892306A3 (en) 2003-10-06 2008-06-11 Bayer HealthCare AG Methods and kits for investigating cancer
WO2005049787A2 (en) * 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
WO2005106012A2 (en) 2004-04-28 2005-11-10 Bayer Healthcare Ag Diagnostics and therapeutics for diseases associated with dipeptidyl-peptidase 1 (dpp1)
GB0418651D0 (en) * 2004-08-20 2004-09-22 Medical Res Council Method
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP2239342A3 (en) 2005-02-01 2010-11-03 AB Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
EP2241637A1 (en) 2005-02-01 2010-10-20 AB Advanced Genetic Analysis Corporation Nucleic acid sequencing by performing successive cycles of duplex extension
WO2006119439A2 (en) * 2005-05-03 2006-11-09 Althea Technologies, Inc. Compositions and methods for the analysis of degraded nucleic acids
CA2636855C (en) 2006-01-11 2016-09-27 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US20080287857A1 (en) * 2006-02-11 2008-11-20 Rune Kjeken Device and method for single-needle in vivo electroporation
CN101370553B (zh) * 2006-02-11 2013-04-10 基因特伦尼克斯公司 用于单针体内电穿孔的装置和方法
US20080045880A1 (en) * 2006-02-11 2008-02-21 Rune Kjeken Device and method for single-needle in vivo electroporation
WO2007111937A1 (en) 2006-03-23 2007-10-04 Applera Corporation Directed enrichment of genomic dna for high-throughput sequencing
US7455844B2 (en) 2006-03-29 2008-11-25 Merial Limited Vaccine against streptococci
AU2007237909A1 (en) * 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
EP2047910B1 (en) 2006-05-11 2012-01-11 Raindance Technologies, Inc. Microfluidic device and method
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2029781A4 (en) * 2006-05-26 2010-06-30 Althea Technologies Inc BIOCHEMICAL ANALYSIS OF PARTITIONED CELLS
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
ATE521704T1 (de) 2007-03-01 2011-09-15 Symphogen As Verfahren zur klonierung verwandter antikörper
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
WO2009017679A2 (en) * 2007-07-31 2009-02-05 Merck & Co., Inc. Igf-1r specific antibodies useful in the detection and diagnosis of cellular proliferative disorders
MX2010002773A (es) 2007-09-12 2010-03-31 Novartis Ag Antigenos mutantes de gas57 y anticuerpos de gas57.
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
BRPI0816785A2 (pt) * 2007-09-14 2017-05-02 Adimab Inc bibliotecas de anticorpos sintéticos racionalmente desenhadas, e, usos para as mesmas
EP2212434A1 (en) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase ligation sequencing
CN104292312A (zh) 2007-12-21 2015-01-21 诺华股份有限公司 链球菌溶血素o的突变形式
WO2009100080A1 (en) 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
EP2100970B1 (en) * 2008-03-13 2017-05-10 National Institute of Immunology Ig genes specific oligonucleotides and uses thereof
US9873957B2 (en) 2008-03-13 2018-01-23 Dyax Corp. Libraries of genetic packages comprising novel HC CDR3 designs
CA2722409C (en) 2008-04-24 2017-12-12 Dyax Corp. Libraries of genetic packages comprising novel hc cdr1, cdr2, and cdr3 and novel lc cdr1, cdr2, and cdr3 designs
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
EP2376089B1 (en) 2008-11-17 2018-03-14 The Regents of the University of Michigan Cancer vaccine compositions and methods of using the same
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
CA2762837C (en) * 2009-05-20 2021-08-03 Novimmune S.A. Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants
RU2569187C2 (ru) 2009-05-29 2015-11-20 МорфоСис АГ Коллекция и способы ее применения
EP2442827B1 (en) 2009-06-16 2016-01-06 The Regents of the University of Michigan Nanoemulsion vaccines
AU2010291902A1 (en) * 2009-09-14 2012-04-05 Dyax Corp. Libraries of genetic packages comprising novel HC CDR3 designs
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2517025B1 (en) 2009-12-23 2019-11-27 Bio-Rad Laboratories, Inc. Methods for reducing the exchange of molecules between droplets
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (en) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digital analyte analysis
NZ623714A (en) 2010-05-14 2015-10-30 Alltech Inc Yeast cell wall components and detection thereof
EP3741883B1 (en) 2010-07-16 2022-12-14 Adimab, LLC Antibody libraries
EP2595754B1 (en) 2010-07-22 2018-04-04 Gencell Biosystems Limited Composite liquid cells
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
US8728981B2 (en) 2010-11-19 2014-05-20 Morphosys Ag Collection and methods for its use
EP2652155B1 (en) * 2010-12-16 2016-11-16 Gigagen, Inc. Methods for massively parallel analysis of nucleic acids in single cells
HUE035281T2 (en) 2011-01-14 2018-05-02 Univ California Therapeutic antibodies to ROR-1 protein and methods of their use
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US20140090094A1 (en) 2011-03-09 2014-03-27 Bayer Intellectual Property Gmbh Modulators of slc22a7
SI2702146T1 (sl) 2011-04-28 2019-06-28 The Board Of Trustees Of The Leland Stanford Junior University Identifikacija polinukleotidov, povezanih z vzorcem
IL276182B (en) 2011-05-17 2022-09-01 Univ Rockefeller Neutralizing antibodies against the human immunodeficiency virus and methods of using them
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
BR112014007353B1 (pt) 2011-09-30 2022-09-13 Chugai Seiyaku Kabushiki Kaisha Biblioteca de anticorpos dependentes de concentração de íon
EP2612916A1 (en) * 2012-01-09 2013-07-10 Universität Zürich Cellular high throughput encapsulation for screening or selection
WO2013111016A2 (en) 2012-01-25 2013-08-01 Gencell Biosystems Limited Biomolecule isolation
EP2626433B1 (en) 2012-02-09 2017-04-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for linking nucleic acids in a microsome from endoplasmatic reticuli
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
MX364957B (es) 2012-08-14 2019-05-15 10X Genomics Inc Composiciones y metodos para microcapsulas.
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20150376609A1 (en) 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013350823B2 (en) 2012-11-27 2017-11-16 Gencell Biosystems Ltd. Handling liquid samples
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2900481A1 (en) 2013-02-08 2014-08-14 10X Genomics, Inc. Polynucleotide barcode generation
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
CN106029885A (zh) 2014-02-10 2016-10-12 基因细胞生物系统有限公司 复合液体池(clc)介导的核酸文库制备装置及其使用方法
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN105085673B (zh) * 2014-05-19 2018-09-11 厦门大学 用于检测柯萨奇病毒a16型病毒实心颗粒的单克隆抗体及其用途
CN107278234A (zh) 2014-10-03 2017-10-20 生命科技公司 基因序列校验组合物、方法和试剂盒
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
US9422547B1 (en) 2015-06-09 2016-08-23 Gigagen, Inc. Recombinant fusion proteins and libraries from immune cell repertoires
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US11173207B2 (en) 2016-05-19 2021-11-16 The Regents Of The University Of Michigan Adjuvant compositions
US11448648B2 (en) 2016-11-11 2022-09-20 Ascendant Diagnostics, LLC Compositions and methods for diagnosing and differentiating systemic juvenile idiopathic arthritis and Kawasaki disease
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US20200339687A1 (en) * 2018-01-19 2020-10-29 The Trustees Of The University Of Pennsylvania Compositions and methods for targeting gamma delta t cells with chimeric antigen receptors
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
US11929171B2 (en) 2018-10-18 2024-03-12 The Board Of Trustees Of The Leland Stanford Junior University Methods for evaluation and treatment of glycemic dysregulation and atherosclerotic cardiovascular disease and applications thereof
EP3941491A4 (en) 2019-03-21 2023-03-29 Gigamune, Inc. ENGINEERED CELLS EXPRESSING ANTIVIRAL T-CELL RECEPTORS AND METHODS OF USE
CN114107449B (zh) * 2020-08-31 2024-04-09 中山大学孙逸仙纪念医院 一种单细胞内原位qPCR方法
WO2024050450A1 (en) 2022-08-31 2024-03-07 Gigamune, Inc. Engineered enveloped vectors and methods of use thereof
CN116590283B (zh) * 2023-07-17 2023-11-07 北京微岩医疗器械有限公司 一种细胞结合增强剂和去除人源游离核酸的方法

Also Published As

Publication number Publication date
DE69228247D1 (de) 1999-03-04
DK0597960T3 (da) 1999-09-13
US5830663A (en) 1998-11-03
CA2114950A1 (en) 1993-02-11
ATE175997T1 (de) 1999-02-15
AU2400192A (en) 1993-03-02
EP0597960B1 (en) 1999-01-20
GR3029913T3 (en) 1999-07-30
JPH06509473A (ja) 1994-10-27
EP0597960A1 (en) 1994-05-25
WO1993003151A1 (en) 1993-02-18
AU665365B2 (en) 1996-01-04
DE69228247T2 (de) 1999-07-08

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