DE69739263D1 - Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten - Google Patents

Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten

Info

Publication number
DE69739263D1
DE69739263D1 DE69739263T DE69739263T DE69739263D1 DE 69739263 D1 DE69739263 D1 DE 69739263D1 DE 69739263 T DE69739263 T DE 69739263T DE 69739263 T DE69739263 T DE 69739263T DE 69739263 D1 DE69739263 D1 DE 69739263D1
Authority
DE
Germany
Prior art keywords
plasmids
pct
coke
proteins
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739263T
Other languages
English (en)
Inventor
Matthew Guy Duchars
Andrew William Topping
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
MSD Biologics UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MSD Biologics UK Ltd filed Critical MSD Biologics UK Ltd
Application granted granted Critical
Publication of DE69739263D1 publication Critical patent/DE69739263D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
DE69739263T 1996-02-22 1997-02-20 Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten Expired - Lifetime DE69739263D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9603803.9A GB9603803D0 (en) 1996-02-22 1996-02-22 Production of proteins, plasmids coding therefor and organisms containing such plasmids
PCT/GB1997/000465 WO1997031118A1 (en) 1996-02-22 1997-02-20 Production of proteins, plasmids coding therefor and organisms containing such plasmids

Publications (1)

Publication Number Publication Date
DE69739263D1 true DE69739263D1 (de) 2009-04-02

Family

ID=10789244

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69739263T Expired - Lifetime DE69739263D1 (de) 1996-02-22 1997-02-20 Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten

Country Status (11)

Country Link
US (1) US6136567A (de)
EP (1) EP0889965B1 (de)
JP (1) JP2000504590A (de)
AT (1) ATE423211T1 (de)
AU (1) AU1884997A (de)
CA (1) CA2247162A1 (de)
DE (1) DE69739263D1 (de)
DK (1) DK0889965T3 (de)
GB (1) GB9603803D0 (de)
WO (1) WO1997031118A1 (de)
ZA (1) ZA971477B (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059297A2 (en) * 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1308517A1 (de) * 2001-10-31 2003-05-07 Aventis Pharmacueticals Products Inc. Vektoren für die Expression von mehrere Transgenen
AU2003237662B2 (en) * 2002-01-25 2008-04-24 Evolva Ltd Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
DK1529110T3 (da) * 2002-08-01 2012-01-30 Evolva Ltd Fremgangsmåde til blanding af store antal af heterologe gener

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD233852B1 (de) * 1984-10-03 1987-11-18 Adw Ddr Verfahren zur herstellung von alpha-amylase
US5017488A (en) * 1986-04-01 1991-05-21 University Of Medicine And Dentistry Of New Jersey Highly efficient dual T7/T3 promoter vector PJKF16 and dual SP6/T3 promoter vector PJFK15
EP0368926A4 (en) * 1987-07-16 1991-11-27 Codon Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same
US5460954A (en) * 1992-04-01 1995-10-24 Cheil Foods & Chemicals, Inc. Production of human proinsulin using a novel vector system

Also Published As

Publication number Publication date
EP0889965B1 (de) 2009-02-18
EP0889965A1 (de) 1999-01-13
US6136567A (en) 2000-10-24
ZA971477B (en) 1997-08-22
GB9603803D0 (en) 1996-04-24
DK0889965T3 (da) 2009-05-18
CA2247162A1 (en) 1997-08-28
WO1997031118A1 (en) 1997-08-28
AU1884997A (en) 1997-09-10
JP2000504590A (ja) 2000-04-18
ATE423211T1 (de) 2009-03-15

Similar Documents

Publication Publication Date Title
NO950534L (no) Modufiserte soppceller og fremgangsmåter for fremstilling av rekombinante produkter
EP0759170A4 (de) Verwendung von grünem fluoreszenzprotein
GR3029913T3 (en) Treatment of cell populations.
DE69636866D1 (en) Reverse "two-hybrid"-systeme
ATE310015T1 (de) Hohe expressionsrate des grün-fluoreszierenden proteins
DK0414870T3 (da) Nitratreduktase som markør for Acremonium Chrysogenum
EP0088195A3 (de) Messenger-RNS dessen Herstellung und Verwendung
DE69413389T2 (de) Trennung von proteinen
ATE216426T1 (de) Verfahren zur isolierung und fusion von zellen
DK0770139T3 (da) En svamp, hvor areA-genet er blevet modificeret, samt et areA-gen fra aspergillus oryzae
AR056723A2 (es) Polipeptido purificado metodo para producirlo , metodo para identificarlo y/ o aislarlo composicion farmaceutica que lo comprende, secuencia de acidos nucleicos aislada que codifica dicho polipeptido, vector que contiene a dicha secuencia celula huesped transfectada por dicho vector, sonda nucleotid
CA2175797A1 (en) Human kunitz-type protease inhibitors
MX9602977A (es) Proteinas que reparan la mala union de adn humano.
DE69739263D1 (de) Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten
AU6724794A (en) GRB3-3 gene, its variants and their uses
ZA97835B (en) Purified sr-p70 protein
DE59712554D1 (de) Rekombinante expression von s-layer-proteinen
DE69425149T2 (de) Verfahren und mittel für die herstellung von bindungsproteinen für inhibitoren von plasmaproteinasen
EP0888374A4 (de) Nukleinsäureliganden mit hoher affinität für die proteine des komplementsystems
TW342413B (en) Novel toxins isolated from Bracon Hebetor
ATE254662T1 (de) Vektoren zum einschaeuse von dns in mykobakterien und verwendung dieser bakterien als impfstoffe
DK0788549T3 (da) Regulerende nukleinsyresekvenser og anvendelser
Lyttleton Nucleic acids: What they are and what they do
SE9302856D0 (sv) Method and means for producing a plasmapeotein-binding protein

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
R082 Change of representative

Ref document number: 889965

Country of ref document: EP

Representative=s name: FDST PATENTANWAELTE FREIER DOERR STAMMLER TSCHIRWI

R081 Change of applicant/patentee

Ref document number: 889965

Country of ref document: EP

Owner name: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LTD., GB

Free format text: FORMER OWNER: AVECIA BIOLOGICS LTD., BLACKLEY, GB

Effective date: 20111121

R082 Change of representative

Ref document number: 889965

Country of ref document: EP

Representative=s name: FDST PATENTANWAELTE FREIER DOERR STAMMLER TSCHIRWI

R082 Change of representative

Ref document number: 889965

Country of ref document: EP

Representative=s name: FDST PATENTANWAELTE FREIER DOERR STAMMLER TSCHIRWI

R081 Change of applicant/patentee

Ref document number: 889965

Country of ref document: EP

Owner name: FUJIFILM DIOSYNTH BIOTECHNOLOGIES UK LTD., GB

Free format text: FORMER OWNER: MSD BIOLOGICS ( UK ) LTD., HODDESDON, GB

Effective date: 20120502

R082 Change of representative

Ref document number: 889965

Country of ref document: EP

Representative=s name: ,

Effective date: 20120502

Ref document number: 889965

Country of ref document: EP

Representative=s name: ,

Effective date: 20120529